80 results about "Piper truncatum" patented technology

The invention relates to a high-efficiency imitative wild cultivation method for Tetrastigma hemsleyanum Diels et Gilg (stellaria alsine Grimm.), which comprises the following steps of: 1) selecting a fertile land or northward terrace land in a mountain area, clearing weeding in fields, and performing fertilizer application; 2) dividing the land subjected to deep tillage into planting belts, forming irrigation ditches between the planting belts, digging drain ditches and operation ditches at the edges of the planting belts, and laying trickle irrigation equipment; 3) performing grass mulching and film mulching on the planting belts; 4) establishing Tetrastigma hemsleyanum Diels et Gilg germchits; 5) in the second year, when the vines of the Tetrastigma hemsleyanum Diels et Gilg grow to 30-50 centimeters long, guiding the vines of the Tetrastigma hemsleyanum Diels et Gilg onto a trellis, and starting three-dimensional planting; 6) in the second year, erecting a steel-structured greenhouse 1.5 meters beyond the planting belts, and covering a shading mesh on the top; and 7) in the fourth or fifth year, harvesting. According to the invention, the artificial field planting of the Tetrastigma hemsleyanum Diels et Gilg is realized; and the advantages of quick putting into production, high yield of underground tubers and overground leaves and vines for medical use, high profit, low planting cost, simple operation process and the like can be achieved.

The invention provides a rapid breeding method for bletilla striata. Bletilla striata seedlings are obtained by the steps of processing of a seedling culture bed, selection and preprocessing of explants, induction of cluster buds, culture of roots of the bletilla striata seedlings, division culture of the bletilla striata, final-period management and the like. The method has the advantages of high suitability for industrial production, excellent formula effect, high bud ratio, short culture time, vigorous growth of plants, high transplanting survival rate, strong operability, high application value and the like and has the characteristics of low investment and high output.

The invention discloses a Chinese yam and citrus mixed planting method. The method includes the steps that 1, planting holes are dug; 2, seedlings are planted; 3, fertilizer is applied; 4, branches and leaves are trimmed, and flowers and fruits are protected; 5, Chinese yams are planted; 6, Chinese yam hole applied fertilizer is applied. The Chinese yams are planted under citruses according to standard line spacing and a standard interval, citrus fallen leaves, sheared branches and leaves and fruits are utilized for providing nutrients for growth of the Chinese yams to dispel grubs, cutworms and other underground insects, and Chinese yam tubers do not need prevention and treatment by chemical pesticides. In addition, the Chinese yams are utilized so that the soil fertilizer loss caused by water and soil loss can be avoided, the citrus branches and leaves can prevent the Chinese yams from being impacted by storm winds and hard rain, and the Chinese yams can avoid that because too much water is accumulated at the roots of the citruses, the roots are rotten; advantages of the citruses and advantages of the Chinese yams can be complementary, growth contradicts of the citruses and Chinese yams are controlled, mutual promotion and mutual benefit are achieved, the survival rate of plants of the citruses and Chinese yams is increased, the yield of fritillary bulbs and snakegourd fruits is increased, and the quality of the fritillary bulbs and snakegourd fruits is improved.

The invention discloses a seed seedling raising method for Paris polyphylla var. yunnanensis(Fr.)hand.-mazz.. By the method, the rate of emergence is increased, and the seedling rate is increased. Paris polyphylla var. yunnanensis(Fr.)hand.-mazz. seeds subjected to stratification treatment and post ripening are uniformly broadcast on a prepared artificial substrate according to 600 / 800 per square meter, and by the loose, air-permeable and water-retention substrate, the good development of root systems in an early Paris polyphylla var. yunnanensis(Fr.)hand.-mazz. seed germination process is ensured, so that the rate of emergence is increased, and can reach more than 80 percent; by leaf dressing, the nutrition of seedlings is added, the growth of tubers of the Paris polyphylla var. yunnanensis(Fr.)hand.-mazz. seed seedlings is promoted, the stress resistance of the seedlings is improved, the seedling rate is greatly increased, and reaches more than 60 percent, the utilization rate of Paris polyphylla var. yunnanensis(Fr.)hand.-mazz. seed resources is increased, the good development of Paris polyphylla var. yunnanensis(Fr.)hand.-mazz. plantation is promoted, and special rare medicinal material resources in China are effectively protected.

The invention provides a method for segmenting bletilla striata tubers to perform seedling propagation and raising. The method comprises the steps of fresh bletilla striata tuber preprocessing, light treatment, low-temperature processing, shoot-inducing processing, root-inducing processing, bletilla striata seedling transplantation, later-period management and the like. The method directly uses the bletilla striata tubers as a culture material, enables explants to be thicker and solider than seed seedlings, better in disease resistance ability and higher in growing speed, also fully ensures the hereditary stability of the bletilla striata seedlings and avoids the phenomenon that the seedlings are variable. The inhabiting effect on other lateral buds of main buds is eliminated through light treatment and segmenting low-temperature processing, production of the bletilla striata buds can be quickly promoted, and the seedling raising cost is greatly reduced. By means of the method, the budding speed is high, and increase rate is high. The method is simple and does not need additional tissue culture devices, the production cost is low, and bletilla striata seedlings can be produced in a large-scale mode and are high in application value.

The invention relates to a polygonatum kingianum tissue culture method. The method comprises the following steps: subjecting high quality tubers to detoxification in February and March, cutting tubers after detoxification, and culturing tuber blocks in an MS culture medium without any hormone; (2) culturing the tuber blocks in the MS culture medium for 18 to 22 days, transferring the non-polluted polygonatum kingianum tubers to a proliferation medium, and culturing the tubers in the proliferation medium; (3) culturing the tubers in the proliferation medium for 35 to 45 days to generate a large amount of callus; (4) culturing the tubers in a bud promoting medium for 23 to 25 days, cutting polygonatum kingianum buds in the bud promoting medium, and grafting the buds to a rooting medium; (5) culturing the buds in the rooting medium for 50 to 60 days to grow roots, and transferring the seedlings to a seedling sunshine greenhouse; (5) after 30 to 35 days in the seedling sunshine greenhouse, taking out the polygonatum kingianum seedling from bottles. The culture time is shortened from two years to a half year, the living rate of polygonatum kingianum seedling can reach 98% or more, and the breeding speed of polygonatum kingianum is effectively improved.

The invention discloses an interplanting method for polygonatum sibiricum and bletilla striata. Steps include: (1) field preparation; (2) seed selection; (3) planting; (4) sunshade net laying; (5) field management. The management of the standardized interplanting method for polygonatum sibiricum and bletilla striata provided by the invention is scientific, no public hazards are produced, and the quality of obtained bletilla striata is high; compared with the conventional bletilla striata cultivation technique, the interplanting method for polygonatum sibiricum and bletilla striata can increase yield per mu by 200 percent, can obtain up to more than 95 percent of first-grade bletilla striata tubers, and has high economic benefit, social benefit and ecological benefit.

The invention provides a seedling growing method for polygonatum sibiricum seeds. The method comprises the following steps: selection, soaking, fine sand treatment, storage, germination acceleration,seeding and seedling-stage management. According to the method provided by the invention, the polygonatum sibiricum seeds are subjected to soaking and fermentation, cold storage on a culture medium and breeding in soil, then the hibernation period of the polygonatum sibiricum seeds can be greatly shortened, and the breeding process is thus shortened; the breeding process is simple, a large quantity of polygonatum sibiricum seedlings can be provided quickly, the tuber propagation of polygonatum sibiricum is reduced, the planting cost is lowered, artificial planting of polygonatum sibiricum is promoted, excavation of wild polygonatum sibiricum is reduced, and the income of the planters is increased; moreover, through the breeding method provided by the invention, the germination rate of theobtained polygonatum sibiricum seeds is relatively high, thus popularization is facilitated.

The invention discloses a standardized cultivation method of rhizoma bletillae, and belongs to the technical field of traditional Chinese medicinal material plantation. The standardized cultivation method of rhizoma bletillae comprises following steps: 1, seedling breeding; 2, field transplanting; 3, field cultivation growing period management; 4, harvesting and processing; and 5, packaging, storing, and transportation. Compared with the prior art, the standardized cultivation method possesses following advantages: 1, the standardized cultivation method is simple and practical, management is convenient, standardized technical specification is provided for steps from seedling breeding to packaging, storing, and transportation of rhizoma bletillae, and the standardized cultivation method is suitable for large-scale popularization and cultivation; 2, a set of rhizoma bletillae seedling breeding methods is provided, a defect of propagation by tuber that seed consumption amount is large is avoided, improvement of rhizoma bletillae seed germination induction, differentiation propagation, and rooting and strong seedling culture conditions is realized, rhizoma bletillae tissue culture plant survival rate is increased; 3, rhizoma bletillae seedling domestication cultivation measures are provided, and domesticated plant survival rate is increased; and 4, rhizoma bletillae culture medium formula ratio, bottle seedling, and domesticated plant seedling emergence standards are standardized, and lack of technology and standards in large-scale production is avoided.

The invention discloses a high-yielding cultivation method for rhizoma pinelliae. The method comprises the steps: selecting intermontane gentle-slope-region small flat land, which is high in altitude and not high in sunshine intensity and is loose, fertile and moist, as a planting base, carrying out sterilization on seed stems prior to sowing so as to lower the occurrence of root rot, adopting tuber spring sowing so as to increase the germination percentage and survival rate of the rhizoma pinelliae, carrying out scientific field management and timely topdressing, carrying out first-time topdressing after seedling emergence, then, carrying out topdressing repeatedly according to conditions of seedlings, carrying out irrigation and drainage earth-up so as to keep soil moist, crushing soil at edges of furrows during earth-up, uniformly spreading crushed soil to furrow faces, and leveling the soil by hands so as to prevent water accumulation; and carrying out timely bud picking, carrying out rational fertilizer application and carrying out disease and insect pest control. The high-yielding cultivation method has the beneficial effects that the seed stems are sterilized prior to sowing so as to lower the occurrence of root rot; the tuber spring sowing is adopted, and the seed stems are strictly selected, so that the germination percentage and survival rate of the rhizoma pinelliae are increased; and scientific field management, rational fertilizer application and disease and insect pest control are carried out, so that the occurrence of diseases and insect pests of the rhizoma pinelliae is lowered, and the yield is increased.

The invention discloses a polygonatum cyrtonema endophyte strain ZJU-C612-1, i.e. Bacillus velezensis ZJU-C612-1 with a preservation number of CCTCC NO: M 2018311. The invention also discloses the uses of the strain in inhibiting the growth of Colletotrichum gloeosporioides, Fusarium oxysporum, C. truncatum, F. graminearum, F. solami, Rhizoctonia. solani and F. oxyporum f. sp. Niveum and promotingthe growth of polygonatum kingianum tissue culture seedlings and polygonatum cyrtonema tubers.

The invention discloses a planting method for interplanting peonies and rhizoma polygonati. The planting method includes preparing lands; digging field planting pits; carrying out field planting; carrying out watering; managing water and fertilizers. The planting method has the advantages that scientific planting density is adopted, different fertilizers can be applied to the peonies and the rhizoma polygonati according to nutrient substance requirements of the peonies and the rhizoma polygonati in different periods, accordingly, the disease-resistant performance of the peonies and the rhizoma polygonati can be enhanced, chloroplast structures can be improved, the chlorophyll contents can be increased, and the photosynthesis can be promoted; flower retention effects can be realized for the peonies, lesion can be prevented, colors of flowers can be increased, and effects of prolonging flowering periods can be realized; nutrient substance absorption of tubers of the rhizoma polygonati can be promoted, the contents of total saponins in the tubers of the rhizoma polygonati can be increased, tuber expansion can be promoted, the quality of the rhizoma polygonati can be improved, and the yield of the rhizoma polygonati can be increased.

The invention discloses a selenium-rich Yangshan Chinese yam planting method. The planting method is characterized in that human excrement and sodium selenite are mixed with water for application, a layer of thin soil is spread, and a layer of rotten pig and cattle manure is further spread to obtain a matrix; and a tuber is planted in the matrix. By supplementing a leaf fertilizer through leaf spray application in the growing period of the Chinese yam to promote the growth of the plant, inorganic selenium is converted into organic selenium in the plant, thereby ensuring that the Chinese yam product coming from the plant is rich in the selenium element.

The invention relates to a cultivation method for interplanting featherleaf rodgersflower in a forest. The cultivation method comprises the following steps: selecting a forest land with the elevation of 2200-2600m and the woodland shading degree of 40-50%; applying 2500-3000kg of animal manures or composts to each mu of area after the soil is prepared and turned; selecting healthy featherleaf rodgersflower tubers with the fresh weight of 50-60g as cultivating seeds; disinfecting the cultivating seeds by use of 600-800 times of 50% carbendazim liquid for 10-30 minutes, and then washing with clean water; from the last ten-day period of October to the middle ten-day period of November, planting in the forest, wherein the line space is 35-40cm and the row space is 17-20cm; after the cultivating seeds grow into 5-7cm seedlings, applying 45kg-55kg of calcium superphosphates and 4-5kg of urea to each mu of area; and topping after no seed is leaved for planting and before the flowers are bloomed. According to the cultivation method, the number of the new radical buds grown on the old roots of the featherleaf rodgersflower is 75.9% higher than the reference average number and the dry weight of the root stocks of the new radical buds after growing for 1 year is 320% higher than the reference average dry weight.

The invention discloses a method for rapidly breeding paris polyphylla, mainly aiming at solving the problem of the existing paris polyphylla tuber breeding way in the prior art that, only one bud can be bred by using one tuber, a smaller root is formed, and growing is slow, so that the demand of the existing market on polyphylla breeding cannot be met. The perennial and robust paris polyphylla rhizomes which are not damaged and do not have plant diseases and insect pests are selected as a provenance for breeding; the method comprises the steps of digging out the fresh paris polyphylla rhizomes, washing and then drying in the air, and cutting off adventitious roots on the rhizomes; after that, washing stem internodes with sterile water, then cutting off buds on the stem internodes, and cutting the stem internodes of the rhizomes into blocky structures; inoculating the blocky structures into a breeding culture medium for culturing; when a first true leaf and five adventitious roots grow on one blocky structure, transplanting the blocky structure into soft soil for growing. After the scheme is adopted, the aims of rapidly breeding the paris polyphylla and enabling the paris polyphylla to take more roots are achieved.

The invention belongs to the field of plant cultivation and discloses an artificial cultivation method of dioscorea zingiberensis. The artificial cultivation method of the dioscorea zingiberensis comprises steps of seed selection, soil preparation, seed sowing, frame erecting, weeding, fertilizer applying, water drainage, pest control, ridging and harvest. Seed stem preprocessing is performed before seed sowing, and a seed stem preprocessing method is that 50% of carbendazol wettable powder is diluted into 1000 times to soak seed stems for 30 minutes or water is utilized to soak the seed stems for 30 minutes. The fertilizer applying comprises base fertilizer applying and additional fertilizer applying, wherein base fertilizers are compound fertilizers of potassium sulfate, and additional fertilizers are leaf fertilizers. The artificial cultivation method of the dioscorea zingiberensis overcomes the shortcoming that the existing artificial cultivation method of the dioscorea zingiberensis is non-standard in cultivation technology and low in harvest yield and saponin content and results in tuber rot due to blind fertilization, and is standard in cultivation technology, capable of avoiding tuber rot and high in harvest yield and saponin content.

The invention discloses a method for planting Polygonatum yunnanensis under a Simao pine forest, which comprises the following steps: step 1, preparing tubers; step 2, selecting and preparing the ground; step 3, planting; and step 4, field management. The method can ensure the good growth of Polygonatum yunnanensis and the qualified planting products, and at the same time, because of the wide distribution and large area of ​​the Simao pine forest, it is helpful for the large-scale planting of Polygonatum yunnanensis.

The invention discloses a Polygonatum interplanting cultivation method. The land is flattened into a plurality of planting units arranged in parallel, and three parallel ridges are formed on each planting unit. The length direction of each ridge is east-west direction; the three ridges are respectively For the first Polygonatum planting ridge, the second bean planting ridge, and the third medicinal material planting ridge; on the first Polygonatum planting ridge, select a small section of tuber with two nodes and one terminal bud, and carry out hole sowing at a plant spacing and row spacing of 20cm, and cover with soil 3 ‑5 cm, water after a little suppression, and then water once every 3‑5 days; plant bean plants on the second bean planting ridge according to the season; plant angelica or Gastrodia elata on the third medicinal material planting ridge Above, field management: during the flowering period of Polygonatum, spray selenium-enriched nutrients evenly on the leaves with a sprayer in the morning and evening in fine weather; in mid-March of the year before the harvest of Polygonatum, apply selenium fertilizer to the soil, and the aboveground part will wither in autumn or harvest in the next spring dig. The method not only reduces the risk of market fluctuations, but also improves the yield and quality of Polygonatum.

The invention provides a soilless lightening and simplifying cultivating method for yam bulbil. The method comprises the steps of material selecting, cultivating groove preparing, seeding, field managing, nutrient and water managing and harvesting. The yam bulbil is planted in a cultivating groove containing with soilless culture substrates, water and nutrient managing is carried out according to different development stages of the yam bulbil, the yam bulbil can rapidly grow, the problems that the yam plating difficulty, the harvesting difficulty and the labor and investment are large are solved, and the restricts of the soil property, the soil microbe damage and the crop rotation are overcome. By utilizing the method, the fresh weight of produced underground tubers ranges from 150 g to 205 g. The produced yams are good in quality, and can be used as potato seeds to be planted in the next year. Fifteen thousand to twenty thousand plants of the yam bulbil are planted in an area of 667 m <2> appropriately, and 30-40 workers needed in the traditional method of harvesting and digging potato seeds are reduced to 4-5 workers.

The invention discloses a tuber breeding method for Bletilla striata. The method comprises the steps of carrying out field selection and preparation, carrying out Bletilla striata planting, carrying out field management, carrying out water and fertilizer management and carrying out harvesting and processing. According to the method, adult seedlings on the market are planted firstly, and then, tubers are applied to breeding. The planting breeding of Bletilla striata is extremely difficult, and the adult seedlings of Bletilla striata are mostly cultured from Bletilla striata seeds in special culture media, so that the adult seedlings of Bletilla striata are relatively expensive. According to the method, the adult seedling breeding of Bletilla striata is changed into tuber breeding, selected tubers are current-growth tubers with tender shoots, and the breeding capability of Bletilla striata is enhanced due to the selection; three tubers are placed in each pit, so that the rate of empty seeds is lowered, land resources are effectively utilized, and the planting cost of Bletilla striata is reduced; and reasonable field selection and preparation, field management and soil fertilizer management also facilitate the growth and breeding of Bletilla striata.

The invention relates to a tissue culture and rapid propagation method of curcuma longa. The method comprises the following steps: taking an underground tuber of the curcuma longa as an explant, cleaning, disinfecting, cleaning again, absorbing the water on the surface of the tuber, and putting the explant into an MS germination culture medium for inducing germination to obtain sterile test-tube plantlets; putting the test-tube plantlets into an MS clustered bud culture medium for breeding cultivation to obtain clustered buds; putting the clustered buds into an MS strong seedling culture medium for cultivation to obtain robust plants; moving the robust plants into a 1 / 2 MS rooting culture medium for rooting culture to obtain complete seedlings with roots; exercising the complete seedlings with roots for 6-10 days, transplanting the seedlings into a sand bed to grow for 27-33 days at first, and then transplanting the seedlings into a big field. The multiplication coefficient of the clustered buds obtained by the culture method is 10-15 times, the rooting percentage of the obtained tissue culture seedlings is 100%, and the survival rate of the transplanting seedling bed is more than 95%; the problem of industrialized seedling production of the curcuma longa can be effectively solved.

The invention discloses a cultivation method for selenium-enriched pueraria lobata. The cultivation method for the selenium-enriched pueraria lobata comprises the following steps: excavating puerarialobata; cutting off from the top to the bottom of the pueraria lobata and keeping the pueraria lobata 8-15cm long to obtain vegetative seedlings; pruning the obtained vegetative seedlings, and keeping1 to 2 vine buds which are strong in roots, then soaking in a soaking solution for 1 to 5 hours and draining to be planted; from June to July during which the pueraria lobata is planted, pruned and subjected to root thinning, spraying 20 to 35ml of a nutrient solution per plant for the first time; spraying 40 to 55ml of the nutrient solution per plant for the second time from August to Septemberduring which tubers of the pueraria lobata are rapidly increased, spraying 15 to 30ml of the nutrient solution per plant for the third time in October. The cultivation method for the selenium-enrichedpueraria lobata disclosed by the invention has the beneficial effects that compared with the traditional seed, cutting and layering cultivation method, the used seedlings further can provide more nutrient supply for pueraria lobata seedlings and can promote the pueraria lobata seedlings to grow healthily even in the case of insufficient fertility, so that the survival rate of the pueraria lobatacultivation is improved.

The present invention discloses a compound preservation method of freshly edible rhizoma gastrodiae. The method comprises the following steps: harvested rhizoma gastrodiae is soaked and washed using acertain concentration of food grade organic acids, after a natural air-drying, the soaked and washed rhizoma gastrodiae is loaded into a perseveration bag at a certain thickness, the bag is tied, then a certain dose of 60 Go-gamma is used to irradiate the rhizoma gastrodiae, the irradiated rhizoma gastrodiae is placed in a cold storage, then the preservation bag is opened, a pre-cooling of the rhizoma gastrodiae at three stages is conducted, after the pre-cooling, the bag is tied immediately, a temperature of the cold storage is kept at 2 plus or minus 0.5 DEG C, a relative humidity is kept at 85%-90%, and during the storage process, ozone is periodically aerated for 4 times a day to enable an ozone concentration in the preservation storage to reach 80 mg / L-120 mg / L each time before stopping. The compound preservation method can effectively prevent diseases of rhizoma gastrodiae rotten diseases, infection of mixed bacteria, etc., maintains nutritional quality of the rhizoma gastrodiae, reduces irradiation doses, and enables an effective storage period of the rhizoma gastrodiae to be 180 days and a commodity rate to reach 85% or more. The method is also suitable for the preservation of digging-damaged rhizoma gastrodiae, and simple, highly efficient, green and safe storage and preservation method of the rhizoma gastrodiae.

The invention provides dendrobium and old hen soup, and relates to the technical field of dendrobium food making. The dendrobium and old hen soup is prepared from, by weight, 20-25 parts of dendrobium, 350-420 parts of old hens, 11-15 parts of Chinese yam, 12-16 parts of fructus lycii, 17-20 parts of pearl barley, 7-10 parts of fresh singer, 7-9 parts of rice wine, 3-5 parts of salt, 8-11 parts of strengthening bags and 1500-2200 parts of water. The dendrobium and old hen soup has nutrient ingredients and health care effects which are not owned by ordinary old hen soup. The dendrobium and old hen soup contains calcium, iron, carotene, thiamine and various vitamins and crude fibers, has a very good nutrition supplementing effect and can not cause malnutrition. The dendrobium and old hen soup has the effects of tonifying deficiency, soothing the nerves, improving eyesight, dispelling the wind, nourishing kidneys, moistening lungs, protecting livers and resisting cancer. The dendrobium and old hen soup can remarkably reduce activity of enzymes which can make human bodies age and has the senescence delaying effect. Meanwhile, Chinese yam tubers are rich in polysaccharide and can stimulate and regulate human immune systems.

The invention discloses a scientific and effective planting method for Paris polyphylla. The scientific and effective planting method comprises steps of land preparation, field planting, management of Paris polyphylla seedlings, and field management. The preparation method concretely comprises the following steps: ploughing the selected planting land block with a ploughing depth of 25 cm or above, carrying out solarization for 0.5 to 1 month, killing pests and bacteria with quick lime, carrying out furrowing according to a width of 100 cm, and after a seedbed is prepared, carrying out disinfection with a disinfectant; cleaning triennial Paris polyphylla seedlings with clear water, carrying out disinfection, carrying out planting according to a row distance of 25 to 35 cm and a plant distance of 15 to 20 cm, carrying out watering, and performing covering with disinfected agricultural and forest wastes with a cover thickness of 5 to 7 cm; after the leaf morphology of the Paris polyphylla seedlings are completely differentiated, carrying out cutting from the position of 3 to 5 cm below the first round of leaves of the Paris polyphylla seedlings; and carrying out tending management, wherein the steps of fertilization, weeding and pest control are adopted. According to the invention, in the period of complete differentiation of the leaf morphology and relatively-high and stable growth of the Paris polyphylla seedlings, reproductive growth and differentiation of the Paris polyphylla are blocked, so the growth of Paris polyphylla tubers is promoted; and by combining a corresponding field management method, the yield of the Paris polyphylla can be greatly improved, and the increase rate of the yield achieves 2.5 times or above.

The invention discloses a tissue-culture quick propagation method of brassica oleracea, and relates to the tissue culture of plants. The tissue-culture quick propagation method comprises the following steps of adopting a basal bud clump of a fascicled leaf at the top end of a corm of the brassica oleracea and a basal axillary bud of a lateral leaf of the corm as explants, preserving part of intumescent tuber, removing a leaf, and washing the basal bud clump, the basal axillary bud and the part of intumescent tuber; moving a test material onto an ultra-clean working platform, and disinfecting a washed small tissue block of the brassica oleracea; cutting the disinfected tuber, with an apical bud or an axillary bud, of the brassica oleracea into the small tissue blocks to serve as the explants, inoculating the small tissue blocks in a culture medium to carry out callus induction; when calluses are formed, moving the small tissue blocks into a proliferation culture medium, making induced buds form, and propagating a clumpy bud; carrying out rooting culture on the obtained induced buds and the clumpy bud; when 3 to 4 root systems, with lengths of 2cm to 3cm, of a seedling grow out and the body of the seedling already shows robustness, taking out the seedling, cleanly cleaning the culture medium attached to a root in clear water, well arranging the cleanly cleaned seedlings, spraying the seedlings to be wet by using the clear water, afterwards, carrying out seedling hardening by using perlite and humus soil as matrixes respectively, and carrying out transplantation and field planting on the seedlings.

The invention discloses a method for relay intercropping Paris polyphylla and shade loving traditional Chinese medicine plants. The method comprises the following step of in the second year of planting the Paris polyphylla, planting 1 to 2 rows of shade loving traditional Chinese medicine plants between the rows of the Paris polyphylla according to a shade loving traditional Chinese medicine plant cultivation season, wherein when the relay intercropped shade loving traditional Chinese medicine plants are recovered, the root parts of the Paris polyphylla cannot be damaged. According to the method provided by the invention, according to the biological characteristics that the Paris polyphylla grows slowly, tubers are smaller from the second year of the planting to the fourth year of the planting, and the Paris polyphylla has smaller plants, the shade loving traditional Chinese medicine plants such as radix notoginseng, rhizoma bletillae or pinellia tenatabreit are replay intercropped during a process from the second year to the fourth year of Paris polyphylla planting, so that the use ratio of a Paris polyphylla planting land is improved, and meanwhile, the income in unit area of a farmer household is also greatly improved. The investment risk of a traditional Chinese medicinal material farmer household is also reduced through reasonably sharing and utilizing a shading shed and pine needles.

The invention discloses a method of adopting candida oliva to promote wound healing of postharvest potato tuber, belongs to the field of agriculture and solves the problem that pesticide residue and environment pollution can be caused by adopting chemical drug to promote wound healing of potato tuber. The method includes following steps: inoculating candida oliva onto a bevel of a nutritional yeast glucose solid culture medium for activation, and using sterile water to prepare yeast suspension liquid; inoculating the yeast suspension liquid into a nutritional yeast glucose liquid culture medium for culture for two times; flushing harvested potato tuber, using sodium hypochloride for disinfection, using deionized water to flush, and drying; uniformly spraying target yeast suspension liquidonto the surface of a wound of the potato tuber, naturally air-drying, and putting the potato tuber into a punched preservation bag for wound healing. Pollution-free candida oliva is adopted to heal the wound of the postharvest potato tuber, has biocontrol effect on postharvest disease and pest and can enhance disease resistance of the potato tuber.

The invention provides a cultivation method for overcoming the continuous cropping obstacle of rhizoma corydalis. The method includes the steps of land selection, soil preparation, planting, fertilization, field management, harvesting and subsequent preservation. Before sowing, selected rhizoma corydalis tubers are soaked in 500 times liquid of 50% carbendazim wettable powder for 1 hour, and the survival rate and germination rate of seeds can be improved significantly. In the fertilization process, a microbial fertilizer, an organic fertilizer and a trace element fertilizer are additionally applied in the field management process, by reducing the release of allelochemicals at the roots of the rhizoma corydalis, the soil environment of the root of the rhizoma corydalis is regulated, high-temperature stress and lesions in the growth process of rhizoma corydalis are effectively prevented, the use of pesticide is reduced, the continuous cropping obstacle is prevented, the safety of rhizoma corydalis is ensured, and the quality and yield of rhizoma corydalis are improved.

The invention discloses a jerusalem artichoke germplasm resource conservation method, and belongs to the technical field of jerusalem artichoke germplasms. The jerusalem artichoke recovery difficulty is large, harvesting cannot be completed, seedling germination of tubers remaining in a field can be achieved directly next year, the germplasm resource conservation purity is not high, the mixing phenomenon is serious, and variety resource conservation and potato seed propagation are directly limited. The method comprises the following steps that seeding pits are selected for germplasm resource conservation; polyvinyl chloride woven bags are laid inside the seeding pits; the polyvinyl chloride woven bags are filled with seed sowing soil; jerusalem artichoke tubers are sown inside the seed sowing soil; the polyvinyl chloride woven bags and the seed sowing soil are taken out for tuber harvesting; according to different varieties, subpackaging is performed, shallow burying and depoting are achieved for seed sowing in the next year. The sufficient soil environment is provided for jerusalem artichoke tuber growth, the tuber growth range is well limited, harvesting and conservation of germplasm resources are promoted, the germplasm resource purity is ensured, and meanwhile the influence of tubers remaining in soil on crops in the next season is avoided.