Tissue-culture quick propagation method of brassica oleracea

Abstract

The invention discloses a tissue-culture quick propagation method of brassica oleracea, and relates to the tissue culture of plants. The tissue-culture quick propagation method comprises the following steps of adopting a basal bud clump of a fascicled leaf at the top end of a corm of the brassica oleracea and a basal axillary bud of a lateral leaf of the corm as explants, preserving part of intumescent tuber, removing a leaf, and washing the basal bud clump, the basal axillary bud and the part of intumescent tuber; moving a test material onto an ultra-clean working platform, and disinfecting a washed small tissue block of the brassica oleracea; cutting the disinfected tuber, with an apical bud or an axillary bud, of the brassica oleracea into the small tissue blocks to serve as the explants, inoculating the small tissue blocks in a culture medium to carry out callus induction; when calluses are formed, moving the small tissue blocks into a proliferation culture medium, making induced buds form, and propagating a clumpy bud; carrying out rooting culture on the obtained induced buds and the clumpy bud; when 3 to 4 root systems, with lengths of 2cm to 3cm, of a seedling grow out and the body of the seedling already shows robustness, taking out the seedling, cleanly cleaning the culture medium attached to a root in clear water, well arranging the cleanly cleaned seedlings, spraying the seedlings to be wet by using the clear water, afterwards, carrying out seedling hardening by using perlite and humus soil as matrixes respectively, and carrying out transplantation and field planting on the seedlings.

Description

technical field [0001] The invention relates to plant tissue culture, in particular to a rapid propagation method of kohlrabi tissue culture. Background technique [0002] Kohlrabi (Brassica oleracea L.var.caulorapa DC.), also known as kohlrabi, kohlrabi and kale head, is a biennial herb of the genus Brassica in the family Brassicaceae. Native to the coasts of the Mediterranean Sea to the North Sea in Europe, it is mutated from leafy cabbage, and the edible part is an enlarged fleshy bulb. Kohlrabi has high nutritional value and medicinal effects. It is not only rich in calcium, phosphorus, iron and other trace elements, but also contains thiocarotene, which can promote the production of anti-cancer enzymes in animal and human cells. Recent studies have shown that kohlrabi bark is used as an adsorbent to treat dye wastewater. After adsorption, it can be used for harmless incineration to avoid secondary pollution, and has broad development prospects. [0003] Under natural ...

AI Technical Summary

Problems solved by technology

[0005] Planting kohlrabi in South China is sensitive to high temperature and high humidity. Soft rot often occurs in the fleshy bulbs of kohlrabi in spring, summer and autumn, and large-scale planting of kohlrabi is also difficult.