Tissue-culture quick propagation method of brassica oleracea

A technology for tissue culture and kohlrabi, applied in the field of plant tissue culture, can solve the problems of soft rot and difficulty in large-scale planting of kohlrabi, achieve high reproduction coefficient, eliminate the phenomenon of plant vitrification, and solve the problem of quality degradation of bottle seedlings. Effect

Inactive Publication Date: 2017-06-13
厦门市园林植物园
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Planting kohlrabi in South China is sensitive to high temperature and high humidity. Soft rot often occur...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Use the clustered bud clusters at the top of kohlrabi bulbs as explants, take some tubers, remove the leaves, rinse with clean water (tap water) for 10 minutes, add detergent in an ultrasonic cleaner for 10 minutes, and wash the detergent with water wash.

[0037] 2. Move the test material to the ultra-clean workbench, immerse the cleaned kohlrabi tissue in 75% ethanol for 30 seconds, rinse with sterile water 3 times, drain the water, and then immerse in 0.1 oz. % mercury liter for 8 minutes, take out sterile water and rinse 5 times, drain the water for later use;

[0038] 3. Cut the sterilized kohlrabi stems with terminal buds into small pieces of 0.5-1.0cm tissue as explants, and inoculate them in the modified MS+6-BA1.0mg / L+NAA0.1mg / L+sucrose 25g / L+ Agar 7g / L, medium pH value 6.0 for callus induction. The culture period is 15 days, the culture temperature is about 25°C, the light intensity is about 1500lux, and the light time is 12h / d.

[0039]Four, after the c...

Embodiment 2

[0043] 1. Use the axillary buds on the kohlrabi bulbs as explants, keep some tubers and petioles, remove the leaves, rinse with clean water (tap water) for 5 minutes, add 50% detergent in an ultrasonic cleaning machine to clean for 10 minutes, and rinse with clean water for 10 minutes. Wash with dish soap.

[0044] 2. Move the test material to the ultra-clean workbench, immerse the cleaned kohlrabi tissue in 75% ethanol for 30 seconds, rinse with sterile water 3 times, drain the water, and then immerse in 0.1 oz. % mercuric chloride for 5 minutes, take out sterile water and rinse 5 times, and drain the water for later use.

[0045] 3. Cut the sterilized kohlrabi axillary bud stem into 1.0-1.5 cm tissue pieces as explants, inoculate them for callus induction and cluster bud culture. The culture period is 15 days, the culture temperature is about 25°C, the light intensity is about 2500lux, and the light time is 12h / d.

[0046] 4. Separate the clustered shoots of kohlrabi plant...

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PUM

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Abstract

The invention discloses a tissue-culture quick propagation method of brassica oleracea, and relates to the tissue culture of plants. The tissue-culture quick propagation method comprises the following steps of adopting a basal bud clump of a fascicled leaf at the top end of a corm of the brassica oleracea and a basal axillary bud of a lateral leaf of the corm as explants, preserving part of intumescent tuber, removing a leaf, and washing the basal bud clump, the basal axillary bud and the part of intumescent tuber; moving a test material onto an ultra-clean working platform, and disinfecting a washed small tissue block of the brassica oleracea; cutting the disinfected tuber, with an apical bud or an axillary bud, of the brassica oleracea into the small tissue blocks to serve as the explants, inoculating the small tissue blocks in a culture medium to carry out callus induction; when calluses are formed, moving the small tissue blocks into a proliferation culture medium, making induced buds form, and propagating a clumpy bud; carrying out rooting culture on the obtained induced buds and the clumpy bud; when 3 to 4 root systems, with lengths of 2cm to 3cm, of a seedling grow out and the body of the seedling already shows robustness, taking out the seedling, cleanly cleaning the culture medium attached to a root in clear water, well arranging the cleanly cleaned seedlings, spraying the seedlings to be wet by using the clear water, afterwards, carrying out seedling hardening by using perlite and humus soil as matrixes respectively, and carrying out transplantation and field planting on the seedlings.

Description

technical field [0001] The invention relates to plant tissue culture, in particular to a rapid propagation method of kohlrabi tissue culture. Background technique [0002] Kohlrabi (Brassica oleracea L.var.caulorapa DC.), also known as kohlrabi, kohlrabi and kale head, is a biennial herb of the genus Brassica in the family Brassicaceae. Native to the coasts of the Mediterranean Sea to the North Sea in Europe, it is mutated from leafy cabbage, and the edible part is an enlarged fleshy bulb. Kohlrabi has high nutritional value and medicinal effects. It is not only rich in calcium, phosphorus, iron and other trace elements, but also contains thiocarotene, which can promote the production of anti-cancer enzymes in animal and human cells. Recent studies have shown that kohlrabi bark is used as an adsorbent to treat dye wastewater. After adsorption, it can be used for harmless incineration to avoid secondary pollution, and has broad development prospects. [0003] Under natural ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 马丽娟蔡邦平张秀英张万旗
Owner 厦门市园林植物园
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