Tissue culture and rapid propagation method of curcuma longa

Abstract

The invention relates to a tissue culture and rapid propagation method of curcuma longa. The method comprises the following steps: taking an underground tuber of the curcuma longa as an explant, cleaning, disinfecting, cleaning again, absorbing the water on the surface of the tuber, and putting the explant into an MS germination culture medium for inducing germination to obtain sterile test-tube plantlets; putting the test-tube plantlets into an MS clustered bud culture medium for breeding cultivation to obtain clustered buds; putting the clustered buds into an MS strong seedling culture medium for cultivation to obtain robust plants; moving the robust plants into a 1/2 MS rooting culture medium for rooting culture to obtain complete seedlings with roots; exercising the complete seedlings with roots for 6-10 days, transplanting the seedlings into a sand bed to grow for 27-33 days at first, and then transplanting the seedlings into a big field. The multiplication coefficient of the clustered buds obtained by the culture method is 10-15 times, the rooting percentage of the obtained tissue culture seedlings is 100%, and the survival rate of the transplanting seedling bed is more than 95%; the problem of industrialized seedling production of the curcuma longa can be effectively solved.

Description

technical field [0001] The invention relates to a plant propagation method, in particular to a rapid propagation method of turmeric tissue culture. Background technique [0002] The medicinal material turmeric, also known as Baodingxiang and turmeric, is a plant in the ginger family. The dry rhizome of turmeric (Curcumalonga L.) has the effects of breaking blood, promoting qi, stimulating menstrual flow, and relieving pain. It is used to treat rheumatism, shoulder and arm pain, and chest pain. , dysmenorrhea and injury bruises and so on. [0003] Modern studies have shown that turmeric rhizomes are mainly curcuminoids, containing sesquiterpenoids, acidic polysaccharides, volatile oils, campesterol and other complex components, which can protect the immune system (anti-inflammatory, wound healing, etc.), digestive system (anti-ulcer, antispasmodic), cardiovascular system (lowering blood pressure, lowering blood fat, anticoagulant) and hepatoprotective and choleretic effects,...

AI Technical Summary

Problems solved by technology

However, turmeric rarely blooms, and most of the seeds are not full. In cultivation, the rhizomes are mainly used for reproduction, which is called "planting ginger", that is, "old mother ginger". However, the old mother ginger cannot be used as a seed. The last mother ginger, that is, the "second mother ginger", therefore, the turmeric and the reproduction speed are slow and the efficiency is low.

So far there is no report on the tissue culture study of curcuma longa