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Polygonatum kingianum tissue culture method

A technology of tissue culture and Polygonatum yunnanensis, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low survival rate, slow reproduction speed of Polygonatum yunnanensis, etc., and achieve the effect of improving the breeding speed.

Active Publication Date: 2016-05-04
PUER YULIN FORESTRY DEV CO LTD
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Problems solved by technology

[0007] The invention provides a method for tissue culture of Polygonatum yunnanensis, which solves the existing problems of slow reproduction speed and low survival rate of Polygonatum yunnanensis

Method used

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  • Polygonatum kingianum tissue culture method

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Embodiment 1

[0032] A method for tissue culture of Polygonatum yunnanensis, comprising the following steps:

[0033] (1) In February and March every year, select excellent tubers for detoxification, cut the tubers after detoxification, and insert them into MS medium without any hormones for cultivation;

[0034] Described detoxification comprises the following steps:

[0035] A. Wash the tubers with buds with clean water first, and then clean the tubers with detergent;

[0036] B. Soak in 0.1% sodium hypochlorite solution for 8-12 minutes, then wash with water;

[0037] C. Go to the ultra-clean workbench, soak in 75% alcohol for 10 seconds, rinse with sterile water once, then soak in 0.1% mercuric solution with a concentration of 0.1% for 12 minutes, rinse with sterile water for 8 times, and absorb with filter paper Dry water, that is to complete the detoxification;

[0038] (2) Cultivate in MS medium for 18-22 days, and transfer non-polluted Polygonatum tuber to value-added culture med...

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Abstract

The invention relates to a polygonatum kingianum tissue culture method. The method comprises the following steps: subjecting high quality tubers to detoxification in February and March, cutting tubers after detoxification, and culturing tuber blocks in an MS culture medium without any hormone; (2) culturing the tuber blocks in the MS culture medium for 18 to 22 days, transferring the non-polluted polygonatum kingianum tubers to a proliferation medium, and culturing the tubers in the proliferation medium; (3) culturing the tubers in the proliferation medium for 35 to 45 days to generate a large amount of callus; (4) culturing the tubers in a bud promoting medium for 23 to 25 days, cutting polygonatum kingianum buds in the bud promoting medium, and grafting the buds to a rooting medium; (5) culturing the buds in the rooting medium for 50 to 60 days to grow roots, and transferring the seedlings to a seedling sunshine greenhouse; (5) after 30 to 35 days in the seedling sunshine greenhouse, taking out the polygonatum kingianum seedling from bottles. The culture time is shortened from two years to a half year, the living rate of polygonatum kingianum seedling can reach 98% or more, and the breeding speed of polygonatum kingianum is effectively improved.

Description

technical field [0001] The invention belongs to the technical field of planting Chinese medicinal materials, in particular to a method for tissue culture of Polygonatum yunnanensis. Background technique [0002] Polygonatum yunnanensis is a herbaceous plant of the genus Polygonatum in the family Liliaceae, and it is one of the three basic plants of Polygonatum commonly used in traditional Chinese medicine. Polygonatum, as a commonly used traditional Chinese medicine, has a history of more than 2,000 years in my country. Its nature and taste are sweet and flat, and it enters the lung, spleen and kidney meridians. Efficacy Buzhongyiqi, moisten the heart and lungs, strengthen the bones and muscles. Control cold and heat due to deficiency, tuberculosis and hemoptysis, lack of food after illness, weakness of muscles and bones, rheumatism pain, wind leprosy and ringworm disease, it is a product for nourishing yin. Pharmacological studies in recent years have shown that, in addit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张朝玉彭刚
Owner PUER YULIN FORESTRY DEV CO LTD
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