10 results about "Dioscorea zingiberensis" patented technology

The invention discloses a method for converting furan steroid saponin in a plant body into spiral steroid saponin and producing saponin, which comprises the following steps of: cleaning and selecting fresh leaves of related plants, cutting into pieces, soaking in cold water twice, and combining solutions; performing suction filtration or centrifugation; obtaining a crude enzyme solution; the method comprises the following steps of: adding the saponin into mixed slurry of dioscorea zingiberensis and the like, converting for 1-72 hours, judging whether conversion is complete or not by using a reagent E and a reagent A in combination with a thin-layer chromatography method, reproducing saponin, and comparing the saponin with saponin produced by a conventional pre-fermentation acid hydrolysis method. The method disclosed by the invention has the advantages that the furan steroid saponin in the dioscorea rhizome group plants is converted into the spiral steroid saponin by utilizing the leaching solution of the fresh leaves of the dioscorea rhizome group plants in China, and the saponin is produced and prepared. Compared with the traditional process, the spiral steroid saponin has the advantages of higher yield, lower acid consumption, lower pollution and shorter process time.

The invention provides a composition with moisturizing and anti-saccharification effects, and the composition comprises the following components: 70-85 parts of water, 8-12 parts of butanediol, 0.2-0.3 part of a tremella sporocarp extract, 1-3 parts of a Japanese yam root extract, 1-3 parts of a lotus root extract, 1-3 parts of an abelmoschus esculentus fruit extract, and 1-3 parts of 1, 2-hexanediol. The composition with the moisturizing and anti-saccharification effects shows remarkable advantages in core performance such as moisturizing, anti-saccharification, stability and transdermal absorption, selection of technological parameters directly influences the final effect, and innovativeness of collaborative optimization of the formula and the technology is verified.

The invention provides an extraction method of diosgenin, which comprises the following steps: step 1, dioscorea zingiberensis rhizome and an extraction solvent are put into a crushing extraction device, the crushing extraction device comprises a feed port, a discharge port, a rotating shaft, crushing blades connected with the rotating shaft and a heater, and the rotating speed of the rotating shaft is 800-900 r / s; step 2, after the rhizome of dioscorea zingiberensis and the extraction solvent are extracted for 1-1.5 h in the crushing extraction device, discharging the mixture from a discharge port, filtering, concentrating the filtrate until no alcohol smell exists, adding distilled water, fully stirring, settling and centrifuging to obtain a precipitate, namely dioscin; 3, dioscin and helicase are put into a reaction container, and an acetic acid-sodium acetate buffer solution is added for hydrolysis; 4, after the hydrolysis reaction is finished, a hydrolysis product is inactivated, suspension is centrifuged, supernate is removed, sediment is washed with distilled water, residues are dried and recrystallized with an organic solvent, and diosgenin is obtained. According to the present invention, the dioscin is extracted from the yam plant by using the crushing extraction device, and the diosgenin is further obtained by using the enzymolysis, such that the extraction method can improve the diosgenin extraction amount, can avoid the use of a large amount of acid, can alleviate the environmental pollution problem, and has good industrial application prospects.

The invention relates to the technical field of wastewater treatment, in particular to a dioscorea zingiberensis saponin wastewater pretreatment system which comprises a data acquisition module used for acquiring wastewater matrix parameters and real-time monitoring parameters; the first processing module is used for calculating a resin saturation index in real time; the second processing module is used for accounting the total adsorption mass of the characteristic components and the COD concentration of the recovered high-value components; the third processing module is used for combining the COD (Chemical Oxygen Demand) concentration of the recycled high-value component and preset sludge accounting parameters to carry out actuarial neutralization on the estimated yield of the sludge; the fourth processing module is used for determining the resource recovery value, the environmental load cost and the total operation cost of the system; the benefit analysis module is used for determining a system net economic benefit index in combination with a preset weight coefficient; the self-adaptive control module is used for reversely adjusting a preset switching threshold in response to the system net economic benefit index; according to the invention, a rigid mode of traditional fixed parameter operation is eliminated, and intelligent operation is realized.

The present invention belongs to the field of biotechnology and discloses a method for green and high-yield production of diosgenin using microorganisms. Specifically, after homogenizing Dioscorea zingiberensis, it is treated with bacterial strain A to release saponins, and then treated with double enzymes to saccharify starch. The filtrate and filter cake are obtained by plate and frame filtration. The filter cake is mixed with water and filtered again, and the filtrates are combined to obtain a Dioscorea zingiberensis sugar solution containing saponins. If the raw material is dry Dioscorea zingiberensis, a complex enzyme is added to the filtrate for treatment, and Dioscorea zingiberensis sugar solution and a saponin-containing precipitate are obtained by centrifugation. If the raw material is fresh ginger, the filtrate is subjected to membrane separation to obtain Dioscorea zingiberensis sugar solution and a saponin concentrate, and the supernatant saponin solution and a saponin-containing precipitate are obtained by centrifugation. The saponin-containing precipitate is extracted with ethanol to obtain a saponin extract. A culture medium is prepared with the supernatant saponin solution and the saponin extract, and treated with bacterial strain B, and a diosgenin-containing precipitate is obtained by centrifugation. The precipitate is dried and refined by solvent extraction to obtain a diosgenin product. The present invention improves the design of the overall process flow, can obtain two types of saponins, has a high saponin extraction rate, and greatly improves the diosgenin yield, which is 1.3 to 1.6 times that of the acid hydrolysis process.

The invention provides a protein from dioscorea zingiberensis as well as a coding gene and application thereof. The protein provided by the invention is selected from at least one of a) and b): a) a protein with an amino acid sequence as shown in SEQ ID NO: 2; and b) a fusion protein obtained by connecting a label to the N terminal and / or C terminal of the protein as shown in SEQ ID NO: 2. The protein provided by the invention has P450 enzyme activity, and experiments verify that co-expression of the protein coding gene and other diosgenin synthesis genes in yeast cells can significantly improve the biosynthesis efficiency of diosgenin, and a key gene resource is provided for biosynthesis of diosgenin.

This invention relates to a method for preparing and applying a fermented product of Bifida Ferment Lysate and Dioscorea Oolong with enhanced moisturizing and anti-aging effects, belonging to the field of microbial fermentation technology. This invention discloses a *Bifidobacterium longum*, the nucleotide sequence of which is shown in SEQ ID NO: 1. This invention utilizes *Bifidobacterium longum* HJ-005 to ferment *Dioscorea opposita* and *Dioscorea opposita* to prepare a *Bifida Ferment Lysate* fermented product of *Dioscorea opposita*, simultaneously increasing the content of absorbable diosgenin, anthocyanins, arginine, and organic selenium, with better effects compared to other lactic acid bacteria. It can significantly reduce the water loss and shrinkage of zebrafish embryos, exhibiting a moisturizing effect, and can significantly promote the expression of zebrafish type I collagen and Elna genes, promoting collagen and elastin regeneration, thus possessing anti-aging effects and can be used as a new raw material for cosmetics.

The present application provides a protein derived from Dioscorea zingiberensis, its encoding gene and application. The protein provided by the present application is selected from at least one of a) and b): a) a protein with an amino acid sequence as shown in SEQ ID NO: 2; b) a fusion protein obtained by connecting a tag to the N terminal and / or C terminal of the protein as shown in SEQ ID NO: 2. The protein provided by the present application has P450 enzyme activity, and experiments have verified that the co-expression of the protein encoding gene and other diosgenin synthesis genes in yeast cells can significantly improve the biosynthesis efficiency of diosgenin, thereby providing a key gene resource for the biosynthesis of diosgenin.

The invention relates to the technical field of functional component microencapsulation, and discloses a dioscorea batatas anthocyanin yeast microcapsule as well as a preparation method and application thereof. The dioscorea batatas is rich in anthocyanin, has remarkable biological activities such as oxidation resistance, inflammation resistance and cardiovascular protection, but is poor in stability and low in bioavailability in a natural state. According to the microcapsule prepared by the invention, the yeast is used as a wall material, and the dioscorea batatas anthocyanin is accurately embedded in the yeast to form microcapsule particles with stable structure and uniform particle size. In the preparation process, key parameters such as the raw material ratio, the reaction temperature and the stirring time are optimized, so that the anthocyanin is efficiently loaded, and the microcapsule has excellent performance. The microcapsule can be used as a natural antioxidant and a coloring agent in the field of food, so that the food quality and safety are improved, and the shelf life is prolonged; in the field of medicine, the compound can be used for developing functional preparations for preventing and treating chronic diseases; in the field of cosmetics, the anti-aging composition can be added into skin care products to exert the anti-oxidation and anti-aging effects, and the market application prospect is extremely wide.

The invention relates to the technical field of biological medicine, and particularly discloses a plant composition and application thereof in preparation of breast enlarging products. The plant composition is prepared from the following raw material components: sunflower seed oil, a dioscorea villosa root extract, a radix puerariae extract, a papaya fruit extract, an acetyl hexapeptide-7 aqueous solution and an African chandelia fruit extract. Researches show that the composition composed of the six raw materials can achieve a synergistic effect and has an excellent breast enlarging effect; therefore, further application value in preparation of a product with a breast enlarging effect by using the compound as an active component is important.