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147 results about "Dioscorea zingiberensis" patented technology

Dioscorea zingiberensis, is a species of yam, a tuberous root vegetable. It has been cultivated in China for the production of diosgenin, an important pharmaceutical intermediate for the synthesis of steroid hormones. The rhizomes also produces steroidal saponins (TSS) as part of a treatment for cardiovascular disease.

Method for fast detecting content of dioscin in dioscorea zingiberensis by utilizing near infrared spectrum technology

The invention relates to a method for fast detecting content of dioscin in dioscorea zingiberensis by utilizing near infrared spectrum technology, which effectively solves the problems that a traditional analysis method is complex, high in production cost, low in efficiency and plenty in raw material waste and comprises utilizing a near infrared spectrometer to scan near infrared absorbing spectrum of a calibration sample set, performing pre-treatment and spectrum area selection to obtain calibration sample set characteristic spectrum, and utilizing high performance liquid chromatography to detect index content of the calibration sample set dioscorea zingiberensis to serve as a reference value; utilizing a partial least squares method to combine the characteristic spectrum of the calibration sample set and the reference value to establish a calibration model, then substituting characteristic spectrum of a validation sample set into the calibration model, and obtaining a night-time ozone profile (NIR) predict value; comparing the NIR predict value with the content of validation sample set dioscorea zingiberensis detected through the high performance liquid chromatography (HPLC), performing validation on the calibration model, crushing a sample to be tested, scanning a near infrared spectrogram of the sample to be tested , and after performing pre-treatment and spectrum area selection, inputting the calibration model. The method for fast detecting the content of the dioscin in the dioscorea zingiberensis by utilizing the near infrared spectrum technology is short in time, fast in speed, accurate and capable of performing detection on line in real time, improving working efficiency and reducing cost.
Owner:HENAN UNIV OF CHINESE MEDICINE

Method for cleaning and producing dioscorea zingiberensis saponin with microbial technology adopted

The invention provides a method for cleaning and producing dioscorea zingiberensis saponin with microbial technology adopted, and belongs to the field of bioengineering. The method solves the problems that in the existing dioscorea zingiberensis industry, wastewater and waste residues are large in drainage amount, and dioscorea zingiberensis starch is not utilized effectively. The method includes the following steps: releasing dioscorea zingiberensis saponin with microorganism adopted firstly, obtaining fermentation liquor containing the dioscorea zingiberensis saponin and the starch; and then processing the starch in the fermentation liquor with a two-enzyme method adopted and obtaining hydrolyzed sugar, and further releasing the dioscorea zingiberensis saponin; conducting solid-liquid separation on dioscorea zingiberensis mash; gathering the hydrolyzed sugar and the dioscorea zingiberensis saponin with microfiltration and nanofiltration membrane separation adopted in sequence, or obtaining intracellular products and dioscorea zingiberensis saponin through specific microbial fermentation separation, and obtaining the dioscorea zingiberensis saponin by hydrolysis of glycosidase and a small amount acid and extraction of a small amount of organic solvent on concentrated dioscorea zingiberensis saponin solution. According to the method, the yield coefficient of the dioscorea zingiberensis saponin is high (90-95%), wastewater quantity is little in the process of production, degree of contamination is low, microbial treatment is low in cost and short in use time, the dioscorea zingiberensis starch is recycled fully, and the method has a great industrial application promotion value in the dioscorea zingiberensis industry.
Owner:HUAZHONG UNIV OF SCI & TECH

Method for remedying heavy metal contaminated soil by combining plants with microorganisms

The invention discloses a method for remedying heavy metal contaminated soil by combining plants with microorganisms. The method comprises the specific steps that (1), the contaminated soil is deep ploughed 40 cm, then meshed pipelines with the diameter of 30-40 mm are buried, and sterilization and disinfection liquid is introduced into the pipelines; (2), a base fertilizer is applied, and a microorganism mixed fungicide and water are mixed according to the mass ratio of 1:5 and then sprayed according to the specification of 20-30 kg per mu; (3), dioscorea zingiberensis is planted, and mixed seeds of vetiver grass and bidens maximowicziana are sown between every two adjacent rows; and (4), the vetiver grass and the bidens maximowicziana which are planted among the dioscorea zingiberensis are rooted up to recycle heavy metal on September to October every year, the seeds are sown again on March to April in the next year, and the dioscorea zingiberensis, the vetiver grass and the bidens maximowicziana are rooted up together after three continuous years to recycle the heavy metal. The soil remediation method is good in remediation effect, low in cost, easy to manage and operate and capable of not generating secondary pollution and has a wide application prospect.
Owner:广州德隆环境检测技术有限公司

Bacillus subtilis SWB8 for producing diosgenin

InactiveCN102061280AAvoid pollutionThe ability to produce diosgenin is stableAntibacterial agentsOrganic active ingredientsBiotechnologyHost plants
The invention discloses a Bacillus subtilis WB8 capable of producing diosgenin, and belongs to the technical field of applied microbiology. The strain is separated from internal issues of subterraneous stems of a medicinal plant of Dioscorea zingiberensis wright, can synthesize and secrete a metabolin of a host plant, namely diosgenin, and has the preservation number of CCTCC (China Center For Type Culture Collection) NO:M2010271; and the strain is rod-like, has the size of 1-1.5*3-5 mu m, forms a single-chain sporocarp, releases endospore, has rough colony with grid bulges, forms pellicle through liquid fermentation, has rich foam, and can grow 16SrDNA core sequence 1542bp with the thermophilic temperature of between 30 and 35 DEG C, the pH of 6.0-9.0 and capacity of tolerating 9.0 percent NaCl, wherein the core sequence has the sequence number of HM210636. The strain liquid is fermented by a common culture medium for 48 to 60 hours, the chloroform extract of the fermentation liquor is the diosgenin, and the diosgenin has the activity of inhibiting pathogenic bacteria and resisting tumor cells. The strain provided by the invention can produce the diosgenin; and compared with the conventional process of extracting the diosgenin from yam plants, the method avoids the pollution of strong acid and yam byproducts on environment, and has potential industrial application prospect.
Owner:SOUTHWEST UNIVERSITY

Biological organic silicon bacterial fertilizer capable of degrading heavy metals with high efficiency, and preparation method thereof

The invention discloses a biological organic silicon bacterial fertilizer capable of degrading heavy metals with high efficiency, and a preparation method thereof. The biological organic silicon bacterial fertilizer is prepared from a fermentation microorganism agent, a functional microorganism agent, an enzyme preparation, a rare earth silicon raw material, straw powder, an amino acid raw powder, abamectin residue, potassium fulvic acid, dioscorea zingiberensis residue, cassava residue, and livestock manure at a certain ratio. The preparation method comprises following steps: 1, cultivation of the activated fermentation microorganism agent; 2, cultivation of the activated functional microorganism agent; 3, fermentation preparation; 4, fermentation; and 5, fermentation postprocessing. The biological organic silicon bacterial fertilizer is capable of reducing heavy metal content of soil, providing plants with abundant trace elements, improving plant biological characters, resisting diseases and insects, and realizing nitrogen fixation, phosphorus dissolving, and potassium releasing. The preparation method is simple; operation is convenient; production cost is low; and the preparation method is suitable for popularization and industrialized large-scale production.
Owner:杨鹏飞

Method for producing diosgenin through microbial fermentation of peltate yam rhizome-yellow ginger

The invention discloses a method for producing diosgenin through microbial conversion of peltate yam rhizome-yellow ginger. Yellow ginger dry powder and tap water are mixed according to a massic volume ratio of 1:5-12, urea which is 4.0% of mass of the yellow ginger dry powder, yeast extract which is 1.0% of mass of the yellow ginger dry powder and inorganic salt which is 0.2% of volume of the tap water are supplemented to prepare conversion slurry, the conversion slurry is introduced in bacillus bacterial strain XBT 2011 seed liquid which is 5% of the volume of the tap water, under the condition that the culture temperature is 40 DEG C, the pH is controlled to be about 6.0, the stirring speed is 300-400rpm and the ventilation condition is 2.5-4.0l / min, the conversion is 56-72hrs, conversion slurry is filtered and dried to obtain hydrolysate, the hydrolysate is extracted for 12-16hrs by using 120# gasoline, the volume of the 120# gasoline is one time of that of the hydrolysate, and diosgenin end products are obtained through repeated crystallization. A preservation number of the bacterial strain XBT 2011 is CGMCCNo.6301. The dry weight of the hydrolysate is 28-33% of that of the added yellow ginger dry powder, total saponins of the peltate yam rhizome is approximately converted into diosgenin completely, handling capacity is big, no sugar contains, the pH is close to neutral, and environmental pollution is reduced.
Owner:西安绿泉科技有限公司

Method for extracting dioscorea zingiberensis pigment by enzymatic method

The invention disclose a method for extracting dioscorea zingiberensis pigment by an enzymatic method, which comprises the following steps of: dissolving pectinase in an acetic acid-sodium acetate solution to prepare a pectinase enzymatic solution; adding dioscorea zingiberensis powder into the pectinase enzymatic solution, then adding the acetic acid-sodium acetate solution for reacting, and adding anhydrous ethyl alcohol; extracting in thermostatic waterbath with the temperature of 65-70 DEG C; repeatedly extracting; merging filtrate; and then concentrating the filtrate to that the concentration of ethyl alcohol is less than 10 percent to obtain the dioscorea zingiberensis pigment. By utilizing the special property of the pectinase, the invention effectively improves pigment extraction amount without environmental pollution during production and lays a foundation for the scale production of the dioscorea zingiberensis pigment. By adopting enzyme to pretreat the dioscorea zingiberensis for damaging cell structures and degrading impurities, the invention has higher pigment obtaining efficiency and pigment quality, and not only alleviates environmental pollution and improves the utilization rate of the dioscorea zingiberensis, but also explores a novel edible natural pigment.
Owner:SHAANXI UNIV OF SCI & TECH

Method for preparing diosgenin from B acid DES (deep eutectic solvent) hydrolysis

The invention belongs to the technical field of plant ingredient extraction, and discloses a method for preparing diosgenin from B acid DES (deep eutectic solvent) hydrolysis. Choline chloride and B acid are heated to a molten state so as to obtain a DES; dioscorea zingiberensis rhizome raw material powder, the DES and petroleum ether are added into a reactor; the temperature is raised to 100 to 130 DEG C; stirring and hydrolysis reaction are preformed; reaction liquid is subjected to filtering or centrifugal separation; a liquid petroleum ether phase is taken; petroleum ether recovered through evaporation; the diosgenin in the rest solid phase is extracted by using water saturation normal butanol solution; the normal butanol and the water are recovered through evaporation; the diosgenin is obtained. The characteristic that the B acid DES and the diosgenin can form strong hydrogen bonds is utilized, so that the DES can realize the selective extraction of the diosgenin from the raw materials for hydrolysis. The method has the advantages that the reaction selectivity is good; the pollution is low; the reaction conditions are mild; the problem of high pollution caused by simultaneoushydrolysis of cellulose and starch in the raw materials into sugar in a conventional hydrolysis process can be solved.
Owner:SOUTH CHINA UNIV OF TECH

Method for preparing diosgenin through microwave-assisted acidic ionic liquid catalysis

The present invention relates to a new method for preparing diosgenin through microwave-assisted acidic ionic liquid catalysis, and belongs to the technical field of agricultural product deep processing and medicine. The method comprises: extracting the total saponin in the rhizome of dioscorea zingiberensis or other dioscoreaceae plants with an ethanol aqueous solution, carrying out catalytic hydrolysis on the saponin in a microwave reaction tank through the co-action of microwaves and an acidic ionic liquid to obtain diosgenin, cooling the reaction liquid, filtering, drying the filtration residue, extracting the diosgenin with petroleum ether, and removing water from the filtrate to obtain the ionic liquid, wherein the recovered ionic liquid can be recycled. According to the present invention, the acidic ionic liquid is used to replace the inorganic acid in the traditional hydrolysis method, such that the problem that a large amount of high-acidity chlorine-containing sewage is discharged is solved; the problems of long time consumption and complex production process of the biological conversion method and the enzyme method are overcome; with the microwave-assisted technology, the reaction time can be significantly shortened; the ionic liquid can be recycled so as to reduce the production cost; and the method is the efficient and green environmental protection diosgenin preparation method.
Owner:JIANGNAN UNIV

Cultivation method of dioscorea zingiberensis C.H.Wright

ActiveCN103703973AIncrease productionIncrease net production valueHorticultureSeeds sourceEconomic benefits
The invention relates to a cultivation method of dioscorea zingiberensis C.H.Wright. According to the cultivation method of the dioscorea zingiberensis C.H.Wright, the yield is high, and the sowing quantity is small. The cultivation method of the dioscorea zingiberensis C.H.Wright comprises the steps of selecting land, carrying out land preparation, selecting seeds, carrying out seeds soaking, carrying out primary planting, carrying out field planting, carrying out water and fertilizer management, carrying out rod insertion and frame erection, carrying out harvesting, and carrying out storage. According to the cultivation method of the dioscorea zingiberensis C.H.Wright, due to the fact that the scientific and reasonable steps of land selection, land preparation, seed selection, seed soaking, primary planting, field planting, water and fertilizer management, rod insertion, frame erection, harvesting and storage are adopted, the yield of the planted dioscorea zingiberensis C.H.Wright can be increased to 3000 kilograms per mu from 2000 kilograms per mu; in addition, the input quantity of a seed source can be reduced to 100 kilograms per mu from 300 kilograms per mu in the prior art, the net production value per mu of the dioscorea zingiberensis C.H.Wright can be greatly increased, the net production value per mu can be increased by 2700 yuan RMB every year, and the economic benefit of farmers for planting the dioscorea zingiberensis C.H.Wright can be greatly improved.
Owner:CHONGQING HOLLEY PHARMA

Batch production method of medicinal dioscorea zingiberensis

The invention discloses an effective and low-energy-consumption rapid propagation method of dioscorea zingiberensis. The method comprises steps as follows: (1) stems of an excellent medicinal dioscorea zingiberensis plant are selected as explants, the explants are disinfected and then cultured in an agar solid culture medium containing MS, 6-BA (0.01-0.05 mg/L), paclobutrazol (3.0-10.0 mg/L) and chlormequat chloride (3.0-10.0 mg/L) and having the content of components excluding inositol unchanged and the content of inositol doubled, and a large quantity of small bud clusters are induced; (2) the cluster buds obtained in the step (1) are cut off and divided into clusters, each cluster comprises five buds, an agar solid culture medium containing MS, NAA (0.02-0.6 mg/L), IAA (0.02-0.6 mg/L), paclobutrazol (0.5-5.0 mg/L) and chlormequat chloride (0.5-5.0 mg/L) and having the content of components excluding inositol unchanged and the content of inositol doubled is inoculated with the buds for proliferation and rooting. The method is good in cultivation effect, high in proliferation coefficient, good in stability and low in production cost and is a rapid propagation method for obtaining a large quantity of test-tube plantlets within the shortest time.
Owner:INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI +1
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