Method for segmenting bletilla striata tubers to perform seedling propagation and raising

A technology of bletilla striata and relative humidity, which is applied in the field of medicinal plant seedling breeding, can solve the problems of low reproduction coefficient of pseudobulbs, low cultivation environment, and short cultivation time, so as to ensure genetic stability, high transplanting survival rate, and application high value effect

Active Publication Date: 2015-12-16
张家界欣农农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] Aiming at the problems that the seedling rate of bletilla striata is extremely low, the reproductive coefficient of conventional pseudobulbs is too low, and the tissue culture seedlings are cultivated, the input is large and the output is not high. The hig

Method used

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  • Method for segmenting bletilla striata tubers to perform seedling propagation and raising

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Select the fresh tubers of Bletilla striata just excavated, remove the fibrous roots, separate each tuber to form a single tuber, and wash it with 5% carbendazim solution. Continuously irradiate for 24 hours with illuminance of 1000-2000LX, keep the relative humidity at 80%, maintain the temperature at about 24°C, stir 6 times halfway, and treat for 8 days to obtain a single tuber of bletilla striata that turns green. Each of the bletilla striata tubers that turn green is divided into 2cm thick fritters 10kg with a knife crosscut (press figure 1 Carry out division bletilla striata tuber), then mix with granular activated carbon 9kg of about 5 orders after sterilization, maintain about 85% at 3 ℃ of relative humidity, carry out low-temperature treatment 12 days. Spray the germ growth-promoting solution once every other day after the low-temperature treatment is taken out. The germ-promoting solution is an aqueous solution containing 2g / L of 6-BA, 0.3g / LNAA, and 3% carben...

Embodiment 2

[0040] Select the fresh tubers of Bletilla striata excavated in mid-September, remove the fibrous roots, separate each tuber to form a single tuber, and wash it with 8% carbendazim solution. Continuously irradiate for 24 hours with illuminance of 1000-2000LX, maintain the relative humidity at about 80%, maintain the temperature at about 22°C, stir 8 times in the middle, and process for 10 days to obtain a single tuber of bletilla striata that turns green. Each of the bletilla striata tubers that turn green is divided into 1.5cm thick fritters 20kg with a knife crosscut (according to figure 1 Carry out as shown), then mix with granular activated carbon 18kg of about 5 meshes after sterilization, maintain the relative humidity of the environment at 75% at 4°C, and carry out low-temperature treatment for 10 days. After the low-temperature treatment is taken out, spray the germ-promoting solution (the aqueous solution of 6-BA, 0.2g / LNAA, 4% carbendazim containing 2g / L) once every ...

Embodiment 3

[0042] Select the fresh tubers of Bletilla striata excavated on August 20, remove the fibrous roots, separate each tuber to form a single tuber, and wash it with 5% to 10% carbendazim solution. Continuously irradiate for 24 hours with an illumination of 1000-2000LX, maintain the relative humidity at 75%, maintain the temperature at about 20°C, turn it 7 times halfway, and treat it for 12 days to obtain a single tuber of bletilla striata that turns green. Each green bletilla striata tuber is divided into 1.5cm thick fritters 10kg with a knife figure 1Carry out as shown, and then mix with 12 kg of granular activated carbon of about 5 mesh after sterilization, and at 0° C., the relative humidity is maintained at 75%, and low-temperature treatment is carried out for 7 days. After the low-temperature treatment is taken out, the aqueous solution (bud growth promoting solution) containing 6-BA, 0.4g / LNAA, and 2% carbendazim of 3g / L is sprayed once every other day, and every 100kg ble...

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Abstract

The invention provides a method for segmenting bletilla striata tubers to perform seedling propagation and raising. The method comprises the steps of fresh bletilla striata tuber preprocessing, light treatment, low-temperature processing, shoot-inducing processing, root-inducing processing, bletilla striata seedling transplantation, later-period management and the like. The method directly uses the bletilla striata tubers as a culture material, enables explants to be thicker and solider than seed seedlings, better in disease resistance ability and higher in growing speed, also fully ensures the hereditary stability of the bletilla striata seedlings and avoids the phenomenon that the seedlings are variable. The inhabiting effect on other lateral buds of main buds is eliminated through light treatment and segmenting low-temperature processing, production of the bletilla striata buds can be quickly promoted, and the seedling raising cost is greatly reduced. By means of the method, the budding speed is high, and increase rate is high. The method is simple and does not need additional tissue culture devices, the production cost is low, and bletilla striata seedlings can be produced in a large-scale mode and are high in application value.

Description

technical field [0001] The invention relates to the field of medicinal plant seedling breeding engineering, in particular to a breeding method for rapidly inducing bletilla striata seedlings by dividing bletilla striata tubers. Background technique [0002] Bletilla striata, also known as Baiji and Ruolan, is a kind of Orchidaceae Baiji, a perennial herb with pseudobulbs, rhizomes, white, plump, and finger-like divergences. Bletilla striata tubers are mainly used for hemoptysis and hematemesis, traumatic bleeding, sores and swelling, and chapped skin. Tuberculosis hemoptysis, ulcer disease bleeding. Its corm contains white and gum, starch, volatile oil and so on. Medicinal, it has the effects of effect, nourishing the lungs, hemostasis, detumescence, etc. It can be applied externally to treat traumatic bleeding, carbuncle, burns, boils, etc. The flowers are beautiful and cultivated for ornamental use. The stem is stout, erect, 30-60 cm high. 3-6 leaves, lanceolate or br...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01C1/00
CPCA01C1/00A01G22/00
Inventor 向华唐克华唐忠海
Owner 张家界欣农农业科技有限公司
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