4results about How to "Maintain genetic stability" patented technology

This invention discloses a drug-resistant Ewing sarcoma cell line, named human Ewing sarcoma cell ES 003, which was deposited at the China Center for Type Culture Collection on March 10, 2026, with accession number CCTCC NO: C202642. The cell line exhibits doxorubicin resistance; specifically, doxorubicin has an IC50 of [missing information - likely referring to an IC50 value] against the human Ewing sarcoma cell ES 003. 50 Above 5μM.

The application discloses a novel polyhydroxyalkanoate (PHA) polymerase (PhaC) which can efficiently polymerize the activated monomer 6-hydroxyhexanoate-CoA (6HHx-CoA) of 6-hydroxyhexanoate (6HHx). The PhaC is co-expressed with a CoA ligase AlkK, and can synthesize poly-epsilon-caprolactone (PCL) from the direct precursor sodium 6-hydroxyhexanoate in a halomonas sp. Halomonas which has deleted endogenous beta-ketothiolase (PhaA), acetoacetyl-CoA reductase (PhaB), PhaC and enoyl-CoA hydratase (FadB).

The application belongs to the technical field of plant cultivation and particularly relates to a Blumea riparia growth medium, a tissue culture medium, a tissue culture method and application. The Blumea riparia growth medium takes MS medium as a basic medium and further comprises 0.2-0.3 mg / L NAA, 0.08-0.16 mg / L DA-6, 10 mg / L vitamin C, 20 mg / L myo-inositol, 30 g / L sucrose and 6 g / L agar. DA-6 and NAA are jointly applied to improve the rooting efficiency, root length and plant height of Blumea riparia. The Blumea riparia germplasm with high content of levorotatory borneol is used as an explant to carry out tissue culture and seedling raising, the Blumea riparia tissue culture seedling has high content of levorotatory borneol, and Blumea riparia stable genetic good seedlings can be produced.

The application provides a recombinant bacillus subtilis strain capable of efficiently degrading ethanol in response to ethanol and anaerobic environment and a construction method thereof. The strain takes bacillus subtilis as a chassis, and a cascade amplification system is constructed. An induction control box is driven by an ethanol or anaerobic response promoter to express T7 RNA polymerase; and a functional expression box is driven by a T7 promoter to express ethanol dehydrogenase and acetaldehyde dehydrogenase genes in series. The application takes flagellum synthesis genes flM as integration sites, realizes chromosomal integration and flM knockout of double expression boxes through homologous recombination, and obtains the recombinant strain bacillus subtilis DE-02. The strain can intelligently respond to ethanol or anaerobic signals in the intestinal tract, efficiently express ethanol degradation enzymes through a T7 cascade system, completely oxidize ethanol into non-toxic acetic acid, and has the advantages of motion loss, no antibiotic dependence, genetic stability and the like, and is suitable for preparing oral alcoholism-relieving probiotic preparations or functional foods.