The invention discloses a tissue culture quick propagation method of Polygonatum kingianum. The method comprises the following steps: explant selection and treatment, induced culture, successive transfer culture, enrichment culture, rooting culture, and acclimatization and transplant. The induced culture medium is composed of MS, 1.0-3.0mg/L6-BA, 0.5-1.0mg/L 2,4-D, 1.0-2.0mg/L gibberellin, 5.0-10.0g/L agar and 20.0-30.0g/L sucrose. The rooting culture medium is composed of 1/2MS, 0.2-1.0mg/L NAA, 0.2-0.6mg/L IBA, 0.2-0.5% of activated carbon, 5-20% of banana, 6.0-8.0g/L agar and 20.0-25.0g/L sucrose. The method effectively reduces the seed/seedling culture time and cost, increases the quick propagation speed, effectively enhances the Polygonatum sibiricum propagation coefficient, and can well keep the hereditary stability of Polygonatum sibiricum; and the survival percent after transplant can reach 98% or above, thereby creating favorable social benefits and economic benefits.