This invention discloses a drug-resistant Ewing sarcomacell line, named human Ewing sarcomacell ES 003, which was deposited at the China Center for Type Culture Collection on March 10, 2026, with accession number CCTCC NO: C202642. The cell line exhibits doxorubicin resistance; specifically, doxorubicin has an IC50 of [missing information - likely referring to an IC50 value] against the human Ewing sarcoma cell ES 003. 50 Above 5μM.