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33results about How to "Prevent variation" patented technology

Rapid propagation method of triarrhena sacchariflora

The invention discloses a rapid propagation method of triarrhena sacchariflora. The rapid propagation method comprises the following steps of: selecting an excellent single plant of the triarrhena sacchariflora; cutting to take a stem section with 1-2 axillary buds as an explant; after sterilization, inoculating the explant in MS+6-BA0-2.0 mg/L culture medium to culture for 3-5 days, wherein the axillary buds start to sprout and a plantlet is formed around 7-9 days; cutting the plantlet and inoculating the plantlet in MS+6-BA 2.0-6.0 mg/L +IAA0.1-0.5 mg/L+NAA0-0.5 mg/L enrichment culture medium, and keeping high-speed increasing by degrees, wherein enrichment coefficient is above 4; synchronously inducing buds and roots on the enrichment culture medium which is added with NAA, wherein rootage rate is above 85 percent, and a complete plant can be formed only within about 35 days; and transplanting a test tube plantlet to natural soil, wherein transplanting survival rate reaches above 90 percent; thus a set of high-frequency and stable regeneration system is established. The method greatly simplifies the operation process, shortens the culturing time, saves the production cost, and provides a seedling growing method with short cycle, high propagation rate and low cost for large scale planting of the triarrhena sacchariflora.
Owner:HUNAN AGRICULTURAL UNIV

Rapid propagation method of nothapodytes pittosporoides

The invention relates to a rapid propagation method of nothapodytes pittosporoides, and the method is characterized in that tissues such as tender shoot sections are cultivated on a murashige and skoog (MS) culture medium, a tender shoot stem section with one to two sprouts is cut as explants, the explants are grafted in MS+6-butyl acrylate (BA) 1.2mg/L+ indole butyric acid (IBA)0.2mg/L culture medium after being sterilized, the bud is germinated in 10 to 15 days after the grafting, seedlings with leaves are formed in 30 days, the seedlings are cut off and transferred onto the MS+6-BA1.2mg/L+IBA0.4mg/L culture medium to keep the high-speed increase, and the propagation coefficient is more than 5. The MS+6-BA0.5mg/L+ naphthyl acetic acid (NAA)1.5mg/L is selected as rooting culture medium, the rooting rate reaches more than 95 percent, and a complete plant can be formed in 30 days. Test-tube plantlets are transplanted in sandy soil, attention is paid on the moisture preservation, the transplanting survival rate can reach up to 93 percent after the test-tube plantlets are transplanted for 45 days, and a high-frequency stable renewable system is established. The method has the advantages of good stability, simplicity and convenience in operation, fast propagation speed, low production cost, industrialization and the like.
Owner:向华
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