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Rapid Artemisia vestita Wall.ex DC breeding method

A rapid, medium technology, applied in the field of plant tissue culture

Active Publication Date: 2014-11-19
西藏坎巴嘎布卫生用品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002]Kambagabu, scientific name "Artemisia vestita Wall.ex DC" belongs to Asteraceae, Artemisia, semi-shrub or shrub; rhizome is thick, woody, The plant has a volatile aroma and is mainly produced in Dingqing, Luozha, Lhasa, Nanmulin, Shigatse and other hillsides, foothills, grasslands, shrubs, and gravel beaches at an altitude of 2600 to 4300 meters. In terms of value, it is recorded in "Yu Tuo Materia Medica" that Kamba Gabu has the effect of reducing swelling, treating sarcoma and intestinal disease; It is recorded that Kamba Gabu is good for kidney disease and tonic; Kamba Gabu is also used as a hemostatic, swelling, and heat-clearing drug in Tibetan medicine, and it is used to treat bleeding, limb swelling, trauma and other diseases caused by various diseases. With the further development of Tibetan medicine research, the current output of wild Kamba Gabu is difficult to meet the increasing demand for medicine. However, due to the high altitude of Kamba Gabu and strict requirements on the production environment, it is difficult to grow in general environments, and artificial introduction, cultivation and breeding have not yet see the report

Method used

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  • Rapid Artemisia vestita Wall.ex DC breeding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Screening of germination medium

[0021] According to the ratio described in Table 1, different concentrations of germination medium were prepared, respectively numbered 1-4.

[0022] Select an excellent single plant of Kambagabu, cut out the stem segment with 2-3 axillary buds as explants for cultivation, and sterilize the explants, inoculate them in No. 1-4 rooting medium respectively, and the seedlings after inoculation The growth conditions are shown in Table 1.

[0023] Table 1 Effects of germination medium with different hormone ratios on Kambagabu differentiated buds

[0024] culture medium 6-BAmg / L IAA mg / L Number of inoculations Multiplication coefficient growth performance 1 0.3 20 5.1 normal, healthy 2 0.6 0.1 20 5.7 normal, healthy 3 0.9 0.2 20 6.0 normal, healthy 4 1.2 0.3 20 5.2 slightly curled leaves,

[0025] The results showed that as the concentration of 6-BA and IAA...

Embodiment 2

[0026] Example 2 Screening medium for rooting

[0027] According to the ratio shown in Table 2, the rooting medium of different concentrations was prepared, numbered 1-5, and the robust seedlings obtained in Example 1 were transplanted into No. 1-5 rooting medium respectively, and the growth status of the seedlings was as shown in Table 2 :

[0028] Table 2 Effects of rooting media with different hormone ratios on the rooting of Kambagabu

[0029]

[0030] The results showed that in MS rooting medium with 0.05mg / LIBA and 0.05mg / L NAA, the rooting time and uniformity were optimal, and the rooting rate could reach 98%.

Embodiment 3

[0032] The medium formula that embodiment relates to:

[0033] Germination medium: add 1.0 mg / L 6-BA to MS medium;

[0034] Germination medium: Add 0.6-1.2 mg / L 6-BA and 0.1-0.2 mg / L IAA to MS medium;

[0035] Rooting medium: add MS medium, 0.05mg / L IBA, 0.05mg / L NAA.

[0036] Materials: The explants are the stems with axillary buds of Kambagabu (Artemisia vestita Wall.ex DC).

[0037] 1. Selection and sterilization of explants: Select an excellent single plant of Kambagabu, cut out the stem segment with 2-3 axillary buds as explants for cultivation, and sterilize the explants: first, use soapy water to sterilize the explants. Clean, then rinse repeatedly with running water, disinfect in 75% alcohol for 30 seconds, rinse with sterile water for 3 times, then disinfect with 0.1% mercury liter for 5-12 minutes, rinse with sterile water for 3-5 times for later use ;

[0038] 2. Germination of axillary buds: Add germination medium to the culture bottle, inoculate the sterilized...

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Abstract

The invention discloses a rapid Artemisia vestita Wall.ex DC breeding method. The rapid Artemisia vestita Wall.ex DC breeding method comprises the following steps: (A) taking stem segments of an Artemisia vestita Wall.ex DC plant with axillary buds as explants, and sterilizing the explants; (B) inoculating the explants into a germinating culture medium, and culturing for 10-12 days to form leaved seedlings of the explants; (C) moving the leaved seedlings to a sprouting culture medium, inducing the leaved seedlings to form adventitious buds, with 20d as a breeding cycle and after at least one breeding cycle, inoculating 2-2.5cm buds in a rooting culture medium, and transplanting after the seedlings grow to complete plants; (D) transplanting the plants with good roots into a field for normal planting. By the rapid Artemisia vestita Wall.ex DC breeding method, operation is simple and convenient, sprouting is fast, the culture time is short, the survival rate of the plants is high, the plant growth is vigorous and industrial production is easy.

Description

technical field [0001] The invention relates to plant tissue culture technology, in particular to a rapid propagation method of kanbagabu. Background technique [0002] Kambagabu, scientific name "Artemisia vestita Wall.ex DC" belongs to Asteraceae, Artemisia, semi-shrub or shrub; the rhizome is thick, woody, and the plant has volatile aroma. It mainly produces Dingqing, Luoza, Lhasa In the hillsides, foothills, grasslands, shrubs, and gravel beaches at an altitude of 2,600 to 4,300 meters, such as Nanmulin, Shigatse, etc., Kanbagabu has extremely high medicinal value. It is recorded in "Yutuo Materia Medica" that Kanbagabu has The effect of reducing swelling, treating sarcoma, and intestinal disease; it is recorded in "Medicine Names" that Kamba Gabu has the effect of stopping bleeding and reducing swelling of the limbs; In Tibetan medicine, cloth is also used as a medicine for hemostasis, detumescence, and heat-clearing. It is used to treat bleeding, swelling of limbs, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 泽仁旺姆尼珍杜运建
Owner 西藏坎巴嘎布卫生用品有限公司
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