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Rapid salvia chinensis reproduction method

A fast-moving technology, applied in the field of bioengineering, to achieve the effect of small footprint, short production cycle and high application value

Inactive Publication Date: 2018-05-15
长沙湘资生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there have been no reports on the success of tissue culture and rapid propagation of Ishimi Chuan at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Material: Iwami perforata axillary bud stems as explants

[0024] 1. Selection and sterilization of explants: Young tissues such as the fresh shoots and stems of Ishimi Chuan, collected from Cili, Hunan, are used as culture materials, washed with detergent and tap water, and rinsed under running water for 10-30 minutes Finally, place it in 75% alcohol for 10-30 seconds, then disinfect it with 0.1% mercuric chloride for 10 minutes, rinse it with sterile water, cut off the discolored part of the explant and set it aside.

[0025] 2. Germination of buds: inoculate the sterilized buds on N6+6-BA1.2mg / L+NAA0.2mg / L medium, the pH value is 5.8, the culture temperature is 23-27°C, and the light is on every day 12 hours, light intensity 1000LX; 6-8 days after inoculation, buds germinate, and leafy seedlings form in about 10 days.

[0026] 3. Cluster bud induction and proliferation culture: Cut off the seedlings and transfer them to N6+6-BA0.5mg / L+IAA0.4mg / L medium until cluster...

Embodiment 2

[0030] Material: Iwami perforata axillary bud stems as explants

[0031]1. Selection and sterilization of explants: Young tissues such as the fresh shoots and stems of Ishimi Chuan, collected from Cili, Hunan, are used as culture materials, washed with detergent and tap water, and rinsed under running water for 10-30 minutes Finally, place it in 75% alcohol for 10-30 seconds, then disinfect it with 0.1% mercuric chloride for 10 minutes, rinse it with sterile water, cut off the discolored part of the explant and set it aside.

[0032] 2. Germination of buds: inoculate the sterilized buds on N6+6-BA1.2mg / L+NAA0.3mg / L medium, the pH value is 5.8, the culture temperature is 23-27°C, and the light is on every day 16 hours, light intensity 1000LX; 7-8 days after inoculation, buds germinate, and leafy seedlings form in about 12 days.

[0033] 3. Cluster bud induction and proliferation culture: Cut off the seedlings and transfer them to N6+6-BA0.5mg / L+IAA0.4mg / L medium until cluster ...

Embodiment 3

[0037] Material: Iwami perforata axillary bud stems as explants

[0038] 1. Selection and sterilization of explants: Young tissues such as the fresh shoots and stems of Ishimi Chuan collected from Sangzhi in Hunan are used as culture materials, washed with detergent and tap water, and rinsed under running water for 10-30 minutes Finally, place it in 75% alcohol for 10-30 seconds, then disinfect it with 0.1% mercuric chloride for 10 minutes, rinse it with sterile water, cut off the discolored part of the explant and set it aside.

[0039] 2. Germination of buds: inoculate the sterilized buds on N6+6-BA1.2mg / L+NAA0.2mg / L medium, the pH value is 5.8, the culture temperature is 23-27°C, and the light is on every day 16 hours, light intensity 1000LX; about 6-8 days after inoculation, the buds germinate, and the seedlings with leaves form about 10 days.

[0040] 3. Cluster bud induction and proliferation culture: Cut off the seedlings and transfer them to N6+6-BA0.5mg / L+IAA0.4mg / L ...

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PUM

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Abstract

The invention provides a rapid salvia chinensis reproduction method which comprises the following steps: selecting explants, sterilizing the explants, germinating buds, inducing cluster buds, performing reproduction culture, performing rooting culture, transplanting test tube seedlings, and the like. As salvia chinensis stems with buds are adopted as the explants, the explants can be relatively easily taken, the explants can be taken in a whole year, and in addition, a great amount of explants can be taken; due to adoption of root buds as the explants, genetic stability is ensured, meanwhile the defects that calluses gradually stop growing along with bud differentiation on a differentiation culture medium and have no reproduction capability in tissue culture, are overcome, and the phenomenon that differentiated seedling variants are caused along with increase of culture generations in the subculture process of calluses, is avoided.

Description

technical field [0001] The invention relates to the field of bioengineering, and relates to plant tissue culture technology, in particular to a tissue culture method of Ishimi Chuan. Background technique [0002] Plants are a natural treasure house of medicines. People have a long history of using medicinal plants. About 75% of the world's population uses plants as a source of medicine for treating and preventing diseases (Xing Jianmin, 2001). Humans have discovered many drugs with high physiological activity from plants, and the drugs derived from plants account for more than 25% of the total amount of drugs (Zheng Guangzhi, 1987). Our country's traditional Chinese herbal medicine has a history of thousands of years, and it is still widely used in our country and many countries and regions. However, because the traditional method of obtaining Chinese herbal medicine is at the cost of collecting and consuming a large amount of wild plant resources, when the amount of collec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 向华
Owner 长沙湘资生物科技有限公司
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