The invention discloses an application of a
Smad6
gene in promoting
reprogramming of fibroblasts into stem cells. According to the application,
Smad2 / 3 activation is inhibited through overexpression of the
Smad6
gene, and up-regulation of important transcription factors C-Myc, Nanog and Oct4 in the
stem cell state is promoted to be maintained and regulated, so that fibroblasts are promoted to be reprogrammed into the stem cells. According to the method, the roe mother
cell extracting solution is adopted as an
inducer to induce fibroblasts to be reprogrammed into the stem cells, and the materials are low in cost, wide in source and safe. According to the application disclosed by the invention, by means of overexpression of the Smad6
gene, fibroblasts induced by a
fish egg mother
cell extracting solution are promoted to be reprogrammed into stem cells, expression of
stem cell related genes C-Myc, Nanog and Oct4 is promoted, activation of a TGF-beta
signal channel is inhibited, expression of TGF-beta
signal channel related genes Smad2, Smad3 and Smad4 is regulated and controlled, and the stem cells are obtained. The efficiency of
reprogramming the somatic cells into the stem cells is improved, and the application of
reprogramming is greatly promoted.