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Rapid Propagation Method of Kambagabu

一种快速、外植体的技术，应用在植物组织培养领域，能够解决难以生长等问题，达到出芽快、应用价值高、成活率高的效果

Active Publication Date: 2016-02-24
TIBET KAMBA GABU SANITARY PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002]Kambagabu, scientific name "ArtemisiavestitaWall.exDC" belongs to Asteraceae, Artemisia, semi-shrub or shrub; the rhizome is thick, woody, and the plant has volatilization Sexual aroma, mainly produced in Dingqing, Luozha, Lhasa, Nanmulin, Shigatse and other hillsides, foothills, grasslands, shrubs, and gravel beaches at an altitude of 2600 to 4300 meters. Kambagabu has extremely high medicinal value. It is recorded in "Yu Tuo Materia Medica" that Kamba Gabu has the effect of reducing swelling, treating sarcoma and intestinal disease; "Medicine Names" records that Kamba Gabu has the effect of stopping bleeding and reducing swelling of limbs; "Blue Glass" records that Kamba Gabu Gabu is good for kidney disease and tonic; in Tibetan medicine, Kamba Gabu is also used as a medicine to stop bleeding, reduce swelling, and clear away heat. It is used to treat bleeding, swelling of limbs, trauma and other diseases caused by various diseases. With the research of Tibetan medicine However, due to the high altitude and strict requirements on the production environment, it is difficult to grow in the general environment, and artificial introduction and breeding have not been reported yet.

Method used

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  • Rapid Propagation Method of Kambagabu

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] According to the ratio described in Table 1, different concentrations of germination medium were prepared, respectively numbered 1-4.

[0021] Select an excellent single plant of Kambagabu, cut out the stem segment with 2-3 axillary buds as explants for cultivation, and sterilize the explants, inoculate them in No. 1-4 rooting medium respectively, and the seedlings after inoculation The growth conditions are shown in Table 1.

[0022] Table 1 Effect of germination medium with different hormone ratios on Kambagabu differentiation buds

[0023] culture medium 6-BAmg / L IAA mg / L Number of inoculations Multiplication coefficient growth performance 1 0.3

20 5.1 normal, healthy 2 0.6 0.1 20 5.7 normal, healthy 3 0.9 0.2 20 6.0 normal, healthy 4 1.2 0.3 20 5.2 slightly curled leaves,

[0024] The results showed that with the gradual increase of the concentration of 6-BA and IAA, the differentiated shoots of Kam...

Embodiment 2

[0026] According to the ratio shown in Table 2, the rooting medium of different concentrations was prepared, numbered 1-5, and the robust seedlings obtained in Example 1 were transplanted into No. 1-5 rooting medium respectively, and the growth status of the seedlings was as shown in Table 2 :

[0027] Table 2 Effects of rooting media with different hormone ratios on the rooting of Kambagabu

[0028]

[0029] The results showed that in the MS rooting medium with 0.05mg / LIBA and 0.05mg / LNAA added, the rooting time and uniformity were optimal, and the rooting rate could reach 98%.

[0030] Example 3

Embodiment 3

[0032] Germination medium: add 1.0mg / L 6-BA to MS medium;

[0033] Germination medium: Add 0.6-1.2mg / L 6-BA and 0.1-0.2mg / L IAA to MS medium;

[0034] Rooting medium: MS medium, 0.05mg / L IBA, 0.05mg / L NAA.

[0035] Material: Explants from the stems with axillary buds of Kampagabu (ArtemisiavestitaWall.exDC).

[0036] 1. Selection and sterilization of explants: Select an excellent single plant of Kambagabu, cut out the stem segment with 2-3 axillary buds as explants for cultivation, and sterilize the explants: first, use soapy water to sterilize the explants. Clean, then rinse repeatedly with running water, disinfect in 75% alcohol for 30 seconds, rinse with sterile water for 3 times, then disinfect with 0.1% mercury liter for 5-12 minutes, rinse with sterile water for 3-5 times for later use ;

[0037] 2. Germination of axillary buds: Add germination medium to the culture bottle, inoculate the sterilized explants in the germination medium, 1 plant / bottle, under the conditio...

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Abstract

The invention discloses a rapid propagation method of kanbagabu, which comprises the following steps: (A) taking the stem section with axillary buds of a kanbagabu plant as an explant, and sterilizing the explant; (B) taking The explants are inoculated to the germination medium and cultured for 10-12 days, and the explants form leafy seedlings; (C) they will be transferred to the germination medium to induce the formation of clustered buds, with 20 days as a multiplication cycle, and at least one multiplication cycle ends Finally, get the buds of 2-2.5 cm and inoculate them in the rooting medium, and transplant after the seedlings form complete plants; (D) get the plants with good root growth and transplant them to the field for conventional cultivation; the method of the present invention is easy to operate, The method has the advantages of fast germination, short cultivation time, high plant survival rate, vigorous plant growth and easy industrial production.

Description

technical field [0001] The invention relates to plant tissue culture technology, in particular to a rapid propagation method of kanbagabu. Background technique [0002] Kambagabu, the scientific name "ArtemisiavestitaWall.exDC" is Compositae, Artemisia, semi-shrub or shrub; the rhizome is thick, woody, and the plant has a volatile aroma. It mainly produces Dingqing, Luozha, Lhasa, Nanmulin In the hillsides, foothills, grasslands, shrubs, and gravel beaches at an altitude of 2,600 to 4,300 meters such as Shigatse, Kambagabu has extremely high medicinal value. It has the effect of treating sarcoma and intestinal disease; it is recorded in "Medicinal Names of Drugs" that Kambagabu has the effect of stopping bleeding and reducing swelling of the limbs; In medicine, it is also used as a medicine for hemostasis, detumescence, and clearing away heat. It is used to treat bleeding, limb swelling, trauma and other diseases caused by various diseases. With the further development of T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 泽仁旺姆尼珍杜运建
Owner TIBET KAMBA GABU SANITARY PROD CO LTD
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