The invention discloses a far-field optical ultrathin lamella imaging
system and method, and relates to the technical field of optical and
biomedical equipment. The imaging
system comprises a
laser, a
light beam modulation module, an objective lens, a micro-fluidic
chip control box, a micro-fluidic
chip, a narrow-band filter, an
imaging lens and an imaging device; the
light beam modulation module modulates a single
laser beam emitted by the
laser, then the modulated single laser beam enters a rear
aperture plane of the objective lens, and then the modulated single laser beam converges into a channel of the micro-fluidic
chip to generate an ultrathin light sheet; the micro-fluidic chip controls the flow of a
cell sample stained with
fluorescence through the micro-fluidic chip control box;
fluorescent light emitted by the cells is collected to the imaging device through the narrow-band filter and the
imaging lens. Each Bessel
light spot in the focusing area of the objective lens can be independently controlled, when the focusing light spots are close to each other, the interference influence is small, and continuous, high-resolution and high-flux lamellar imaging can be carried out on the
fluorescence sample.