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Quick propagation method for humulus scandens

A five-clawed, fast technology, applied in the field of bioengineering, to achieve the effects of less floor space, manpower saving, and small individual differences

Inactive Publication Date: 2012-07-25
董爱文
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there have been no reports on the success of tissue culture and rapid propagation of Penticodactylus at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Material: explants from axillary bud stems of Pentaclaw

[0025] 1. Selection and sterilization of explants: Young tissues such as the fresh shoots and stems of Pentadactylum collected from Zhangjiajie, Hunan, as culture materials, washed with detergent and tap water, rinsed under running water for 10-30 minutes , placed in 75% alcohol for 10-30 seconds, then sterilized with 0.1% mercuric chloride for 10 minutes, rinsed with sterile water, and cut off the discolored part of the explant for later use.

[0026] 2. Germination of buds: Inoculate the sterilized buds on LS+6-BA1.2mg / L+NAA0.2mg / L medium, the pH value is 5.8, the culture temperature is 23-27°C, and the light is on every day 12 hours, light intensity 1000LX; 6-8 days after inoculation, buds germinate, and leafy seedlings form in about 10 days.

[0027] 3. Cluster bud induction and proliferation culture: Cut off the seedlings and transfer to LS+6-BA0.5mg / L+IAA0.4mg / L medium until cluster shoots are formed, the ...

Embodiment 2

[0032] Material: explants from the rhizome of Pentaclaw with buds

[0033] 1. Selection and sterilization of explants: Young tissues such as the fresh shoots and stems of Pentadactylum collected from Zhangjiajie, Hunan, as culture materials, washed with detergent and tap water, rinsed under running water for 10-30 minutes , placed in 75% alcohol for 10-30 seconds, then sterilized with 0.1% mercuric chloride for 10 minutes, rinsed with sterile water, and cut off the discolored part of the explant for later use.

[0034] 2. Germination of buds: Inoculate the sterilized buds on LS+6-BA1.2mg / L+NAA0.3mg / L medium, the pH value is 5.8, the culture temperature is 23-27°C, and the light is on every day 16 hours, light intensity 1000LX; 7-8 days after inoculation, buds germinate, and leafy seedlings form in about 12 days.

[0035] 3. Cluster bud induction and proliferation culture: cut the seedlings and transfer them to LS+6-BA0.5mg / L+IAA0.4mg / L medium until cluster shoots are formed, th...

Embodiment 3

[0040] Material: explants from the rhizome of Pentaclaw with buds

[0041] 1. Selection and sterilization of explants: Young tissues such as the fresh shoots and stems of Pentadactylum collected from Zhangjiajie, Hunan, as culture materials, washed with detergent and tap water, rinsed under running water for 10-30 minutes , placed in 75% alcohol for 10-30 seconds, then sterilized with 0.1% mercuric chloride for 10 minutes, rinsed with sterile water, and cut off the discolored part of the explant for later use.

[0042] 2. Germination of buds: Inoculate the sterilized buds on LS+6-BA1.2mg / L+NAA0.2mg / L medium, the pH value is 5.8, the culture temperature is 23-27°C, and the light is on every day 16 hours, light intensity 1000LX; about 6-8 days after inoculation, the buds germinate, and the seedlings with leaves form about 10 days.

[0043] 3. Cluster bud induction and proliferation culture: Cut off the seedlings and transfer to LS+6-BA0.5mg / L+IAA0.4mg / L medium until cluster sho...

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Abstract

The invention discloses a quick propagation method for humulus scandens. The method comprises the following steps of: culturing tissues, such as stem section of the humulus scandens, on an LS culture medium; accurately cutting and taking the stem section with 1-2 axillary buds as an explant; disinfecting and then inoculating in an LS+6-BA 1.2mg / L+NAA 0.2mg / L culture medium for culturing; wherein the explant buds after being inoculated for about 6-8 days and grows into a plantlet with leaves after being inoculated for about 10 days; cutting off the plantlet and transferring to an LS+6-BA 0.5mg / L+IAA 0.4mg / L culture medium, so as to keep high-speed increasing and a propagation coefficient above 5; selecting LS+6-BA 0.5mg / L+IAA 0.4mg / L+NAA 0.5mg / L as a rooting culture medium, wherein the rooting rate is above 90% and the complete plant can be obtained only after about 25 days; and transplanting a test-tube plantlet into sandy soil, carefully keeping water and humidity, and establishing a set of high-frequency stable regeneration system after the transplanting survival rate is above 90% after transplanting for 1 month. The method provided by the invention has the advantages of excellent stability, convenience in operation, high propagation speed and low production cost, achieves industrial level, and the like.

Description

technical field [0001] The invention relates to the field of bioengineering, and relates to a plant tissue culture technology, in particular to a method for tissue culture of C. pentonychus. Background technique [0002] Plants are a natural treasure house of medicines. People have a long history of using medicinal plants. About 75% of the world's population uses plants as a source of medicine for treating and preventing diseases (Xing Jianmin, 2001). Humans have discovered many drugs with high physiological activity from plants, and the drugs derived from plants account for more than 25% of the total amount of drugs (Zheng Guangzhi, 1987). Our country's traditional Chinese herbal medicine has a history of thousands of years, and it is still widely used in our country and many countries and regions. However, because the traditional method of obtaining Chinese herbal medicine is at the cost of collecting and consuming a large amount of wild plant resources, when the amount o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 覃静萍
Owner 董爱文
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