Rapid propagation method of triarrhena sacchariflora

Abstract

The invention discloses a rapid propagation method of triarrhena sacchariflora. The rapid propagation method comprises the following steps of: selecting an excellent single plant of the triarrhena sacchariflora; cutting to take a stem section with 1-2 axillary buds as an explant; after sterilization, inoculating the explant in MS+6-BA0-2.0 mg / L culture medium to culture for 3-5 days, wherein the axillary buds start to sprout and a plantlet is formed around 7-9 days; cutting the plantlet and inoculating the plantlet in MS+6-BA 2.0-6.0 mg / L +IAA0.1-0.5 mg / L+NAA0-0.5 mg / L enrichment culture medium, and keeping high-speed increasing by degrees, wherein enrichment coefficient is above 4; synchronously inducing buds and roots on the enrichment culture medium which is added with NAA, wherein rootage rate is above 85 percent, and a complete plant can be formed only within about 35 days; and transplanting a test tube plantlet to natural soil, wherein transplanting survival rate reaches above 90 percent; thus a set of high-frequency and stable regeneration system is established. The method greatly simplifies the operation process, shortens the culturing time, saves the production cost, and provides a seedling growing method with short cycle, high propagation rate and low cost for large scale planting of the triarrhena sacchariflora.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to plant tissue culture technology, in particular to a tissue culture method of Digi and Nandi. Background technique [0002] Obtained [Miscamhus sacchariflorus (Maxim) Benthet Hook. ] Belongs to Poaceae, Gramineae, Panicum, Sorghum, Sugarcane, Miscanthus. Grass is a perennial amphibious plant in the flood storage lake area of ​​the Yangtze River. It is an important fiber raw material for papermaking in my country. Its fiber quality and output are better than those of reed (Phragmites australis L.). Can be used as medicine. At present, the "new economic crops" that have been included in the "new economic crops" that have the potential for utilization and development have been researched by the state. China is the distribution center of harvested plant resources in the world. Through years of research, it has been proved that the genus Graggio It is a kind of plant resource with both ec...

AI Technical Summary

Problems solved by technology

The new species of Nanhuo which is a special product in my country, but for a long time, only a small number of scientific researchers have carried out the research on grass. At present, there are a small number of reports on the tissue culture of grass. , 1988(4)), the shoot tip needs to be stripped under a solid microscope, the operation is complicated, and the explant donors are limited

People such as He Lizhen once used the callus induction and plant regeneration of different explants such as aseptic seedling, hypocotyl, root, sprout coleoptile and young ear to do with report (Northwest Botanical Journal, 1995, 15 (4)), have A process of first cultivating sterile seedlings, then taking the hypocotyls, roots, and coleoptiles of the seedlings to induce callus, and then using the callus to induce regenerated seedlings. The cycle is long and regeneration plants cannot be obtained quickly; As an explant, the panicle is limited by the time of plant booting, and the donor of the explant is limited. Similarly, it also needs to induce the callus, and then induce the regeneration of the seedling with the callus. It also has the disadvantage of a long culture period.

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