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Mass differentiation and rooting method of protocorms of dendrobium candidum

A technology of Dendrobium candidum and protocorm, which is applied in the biological field, can solve the problems of low reproduction coefficient, long growth cycle of Dendrobium candidum, and industrialization restrictions of Dendrobium candidum, and achieve genetic stability, suitable for large-scale production, and reliable technical support. Effect

Inactive Publication Date: 2018-03-16
DALIAN JINGUIYUAN TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low reproduction coefficient of Dendrobium candidum under natural conditions, coupled with the long growth cycle of Dendrobium candidum and the excessive harvesting and destruction of wild resources, wild Dendrobium candidum is on the verge of extinction.
These drawbacks have greatly restricted the industrialization of Dendrobium candidum, which cannot meet the growing market demand.

Method used

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  • Mass differentiation and rooting method of protocorms of dendrobium candidum
  • Mass differentiation and rooting method of protocorms of dendrobium candidum
  • Mass differentiation and rooting method of protocorms of dendrobium candidum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Cultivation of Dendrobium candidum seedlings

[0024] The method of mass differentiation and rooting of Dendrobium officinale protocorm, the specific steps include:

[0025] (1) Obtaining sterile explants: soak the capsules of Dendrobium candidum with cleansing powder for 3 minutes, then remove them and rinse them with tap water, disinfect the surface with 70vt% alcohol under aseptic conditions for 1.5 minutes, and rinse with sterile water for 1 minute, then Soaked in 2wt% sodium hypochlorite solution for 10 minutes, and finally washed 4 times with sterile water, 1 minute each time. After washing, use sterile filter paper to absorb the water on the surface of Dendrobium officinale capsules, and then inoculate it in a proliferation medium for 30 days. Light green with yellowish protocorm; culture conditions are culture temperature 22℃; light time 10h / d, light intensity 2000lx; the formula of the proliferation medium is as follows: 1 / 2MS medium, 0.2mg 1-naphthaleneac...

Embodiment 2

[0028] Example 2 Cultivation of Dendrobium candidum seedlings

[0029] The method of mass differentiation and rooting of Dendrobium officinale protocorm, the specific steps include:

[0030] (1) Obtaining sterile explants: soak the capsules of Dendrobium candidum with cleansing powder for 3 minutes, then remove them and rinse them with tap water, disinfect the surface with 70vt% alcohol under aseptic conditions for 1.5 minutes, and rinse with sterile water for 1 minute, then Soaked in 2wt% sodium hypochlorite solution for 15min, and finally washed 5 times with sterile water, 1min each time, after washing, use sterile filter paper to absorb the water on the surface of Dendrobium officinale capsules, and then inoculate it in the proliferation medium and cultivate for 30 days. Light green with yellowish protocorm; culture conditions are culture temperature 22℃; light time 12h / d, light intensity 2000lx; the formula of the proliferation medium is as follows: 1 / 2MS medium, 0.2mg 1-naphth...

Embodiment 3

[0033] Example 3 Cultivation of seedlings of Dendrobium candidum

[0034] The method of mass differentiation and rooting of Dendrobium officinale protocorm, the specific steps include:

[0035] (1) Obtaining sterile explants: soak the capsules of Dendrobium officinale with cleaning powder for 3 minutes, then remove them and rinse them with tap water, disinfect the surface with 70vt% alcohol under aseptic conditions for 1.5 minutes, and rinse with sterile water for 1 minute. Soaked in 2wt% sodium hypochlorite solution for 15min, and finally washed 5 times with sterile water, 1min each time. After washing, use sterile filter paper to absorb water on the surface of Dendrobium officinale capsules, and then inoculate it in a proliferation medium for 30 days after growing Light green with yellowish protocorm; culture conditions are culture temperature 22℃; light time 12h / d, light intensity 2000lx; the formula of the proliferation medium is as follows: 1 / 2MS medium, 0.2mg 1-naphthaleneace...

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Abstract

The invention relates to the biologically technical field, in particular to a mass differentiation and rooting method of protocorms of dendrobium candidum. The invention adopts the main technical scheme that the mass differentiation and rooting method comprises the following steps: sterile explant obtaining: soaking dendrobium candidum capsules with a cleaning powder for 3 minutes, then fishing out, washing with tap water, sterilizing the surface with 70 weight percent of alcohol for 1.5 minutes under aseptic conditions, cleaning with sterile water for 1 minute, then soaking with 2 weight percent of sodium hypochlorite solution for 10-15 minutes, finally cleaning with sterile water for 4-5 times for 1 minute at a time and after the end of washing, absorbing moisture on the surfaces of thedendrobium candidum capsules with sterile filter paper. The mass differentiation and rooting method provided by the invention has the benefits that based on the study on rapid propagation of mass differentiation and rooting of the protocorms of the dendrobium candidum, the rapid test tube propagation can be performed, so that a reliable technical support is provided for the oriented large-scale industrial production of the dendrobium candidum; the method is simple and practical, efficient and fast, and suitable for large-scale production.

Description

Technical field [0001] The invention belongs to the field of biotechnology and relates to a method for mass differentiation and rooting of Dendrobium officinale protocorm. Background technique [0002] Dendrobium officinale is a perennial herb of the orchid family Dendrobium genus, and it is a traditional precious Chinese medicinal material. Dendrobium officinale contains a variety of effective ingredients, which can be roughly divided into polysaccharides, stillnoids, alkaloids, amino acids and trace elements. Among them, polysaccharides, alkaloids and amino acids have the effects of improving human immune function, anti-oxidation, inhibiting tumors and lowering blood sugar. Dendrobium officinale polysaccharide, its monosaccharide component is composed of D-xylose, L-arabinose and D-glucose, and the polysaccharide content in plants is as high as 22.7%. There are relatively many types of stilbene compounds. At present, the compounds that have been isolated and identified from D...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李春斌李小莹张欣马洪丰张忠军
Owner DALIAN JINGUIYUAN TECH DEV
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