Method for rapidly propagating wild ginseng

A wild ginseng, rapid technology, applied in the field of rapid propagation of wild ginseng, can solve the problems of poor plant quality, time-consuming, complicated steps, etc., and achieve the effect of quality and quality improvement, fast speed, and low mutation rate

Inactive Publication Date: 2012-08-01
CHANGSHU JIASHENG AGRI SCI & TECH DEV
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Problems solved by technology

[0003] At present, the research on ginseng culture mainly focuses on cell culture. Although the existing technology can obtain ginseng regenerated plants by means of callus induction and adventitious embryogenesis, the steps mentioned above are complicated, time-consuming, and the quality of the obtained plants is poor.

Method used

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Embodiment Construction

[0018] The preferred embodiments of the present invention are described in detail below, so that the advantages and features of the present invention can be more easily understood by those skilled in the art, so as to define the protection scope of the present invention more clearly.

[0019] A method for rapid propagation of wild ginseng, comprising:

[0020] (1) Selection and disinfection of explants: cut off the petiole of wild ginseng that grows vigorously from June to July; rinse with running water for 20 minutes, irradiate with ultraviolet light for 5 minutes, transfer to ultra-clean workbench, and work in ultra-clean Soak the table with 70% ethanol for 20-30 seconds, then rinse with sterile water, and then soak in 0.1% mercuric chloride solution with 2 to 3 drops of earth temperature 80 for 3-4 minutes, and use sterile Rinse with water for 3 to 4 times, and finally divide the material under sterile conditions, and cut the petiole into 0.3 to 0.4 cm long;

[0021] (2) C...

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Abstract

The invention discloses a method for rapidly propagating wild ginseng. The method mainly comprises the following steps of: inducing callus, differentiating buds, performing successive transfer culture, performing multiplication culture, performing rooting culture, and hardening seedlings and transplanting. Good effects of high propagation speed, stable hereditary character, high propagation coefficient and eugenic test-tube seedlings are achieved; and through culture, the germination rate of the callus of the wild ginseng is 95 percent, the proliferation times is 4.8 times, the rooting percentage is 96 percent, the final survival rate is 98 percent and the quality is well ensured.

Description

technical field [0001] The invention relates to a method for rapid propagation of wild ginseng. Background technique [0002] Ginseng is a traditional precious Chinese medicinal material in my country. Its active ingredient is ginsenoside, which has the functions of invigorating qi, benefiting lungs, strengthening, nourishing, and anti-fatigue. With the development of society, the market demand for ginseng is increasing day by day. At present, the contradiction between supply and demand of ginseng can be alleviated by artificially adjusting the growth of ginseng cell culture and the production of saponin through cell engineering technology. Wild ginseng grows naturally in the old forests of the original forests, some of which are as long as a hundred years old. They have not been artificially cultivated and have no chemical fertilizers. The collection is very laborious and has high medicinal value. Green rare treasures, valuable. After years of excavation, it has been red...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈勇明
Owner CHANGSHU JIASHENG AGRI SCI & TECH DEV
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