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A method for rapid evoking adventive bud panax ginseng stem segment

A fast technology for ginseng stems, applied in the field of fast obtaining adventitious buds of ginseng, can solve the problems of time-consuming and complicated steps, and achieve the effects of fast speed, small variation of somaclonal lines, and maintenance of genetic stability

Inactive Publication Date: 2010-09-08
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Research on ginseng tissue culture is still rare. Chinese patents 86106832 and 89106227.0 disclose the method of using ginseng roots as explants to obtain ginseng regeneration plants through callus induction and adventitious embryogenesis. However, the regeneration of plants through callus culture The steps are complicated and time-consuming

Method used

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  • A method for rapid evoking adventive bud panax ginseng stem segment
  • A method for rapid evoking adventive bud panax ginseng stem segment
  • A method for rapid evoking adventive bud panax ginseng stem segment

Examples

Experimental program
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Effect test

Embodiment 1

[0017] a. Select 10 ginseng seeds, soak them with 75% ethanol for 30s on the ultra-clean workbench, wash them with sterile water for 4 times, and use 0.1% HgCl 2 Soak in the solution for 8 minutes, then rinse with sterile water for 4 times, inoculate on 1 / 2 MS medium and cultivate for 35 days to germinate the seeds and form ginseng aseptic seedlings;

[0018] b. Remove the leaves and roots of ginseng aseptic seedlings, cut the stem into 1cm segments, insert them into the adventitious bud induction medium to induce adventitious buds, and the adventitious bud induction medium is MS medium + 3% sucrose + 2,4- D 0.2mg / L+NAA 0.5mg / L+6-BA 1.0mg / L+agar 0.7%, the pH value is 5.9, the culture temperature is 20°C, the humidity is 40%, the light intensity is 2000lux, and the light time is 14 hours / After 15 days of culturing, the stem segment began to appear bud point protrusions, and adventitious buds grew after 25 days, and each stem segment explant could induce 1-3 adventitious buds; ...

Embodiment 2

[0021] a. Select 10 ginseng seeds, soak them with 75% ethanol for 25s on the ultra-clean workbench, wash them with sterile water for 3 times, and use 0.2% HgCl 2 Soak in the solution for 6 minutes, rinse 5 times with sterile water, inoculate on 1 / 2 MS medium and cultivate for 30 days to germinate the seeds and form ginseng sterile seedlings;

[0022] b. Remove the leaves and roots of the ginseng sterile seedlings, cut the stems into 1.5cm segments, insert them into the adventitious bud induction medium to induce adventitious buds, and the adventitious bud induction medium is MS medium + 3% sucrose + 2,4 -D 0.1mg / L+NAA 1.0mg / L+6-BA 0.5mg / L+agar 0.8%, the pH value is 5.8, the culture temperature is 22°C, the humidity is 50%, the light intensity is 2300lux, and the light time is 14 hours / day, after culturing for 20 days, the stem segment begins to appear bud point protrusions, and after 30 days, adventitious buds grow, and each stem segment explant can induce 1-3 adventitious bu...

Embodiment 3

[0025] a. Select 10 ginseng seeds, soak them with 75% ethanol for 35s on the ultra-clean workbench, wash them with sterile water for 5 times, and use 0.1% HgCl 2 Soak in the solution for 10 minutes, then rinse with sterile water for 3 times, inoculate on 1 / 2 MS medium and cultivate for 45 days to germinate the seeds and form ginseng aseptic seedlings;

[0026] b. Remove the leaves and roots of ginseng aseptic seedlings, cut the stem into 2cm segments, and insert them into the adventitious bud induction medium to induce adventitious buds. The adventitious bud induction medium is MS medium+sucrose 3%+2,4- D 0.3mg / L+NAA 0.1mg / L+6-BA 2.0mg / L+agar 0.6%, the pH value is 6.0, the culture temperature is 25°C, the humidity is 60%, the light intensity is 2500lux, and the light time is 14 hours / After 15 days of culturing, the stem segment began to appear bud point protrusions, and adventitious buds grew after 25 days, and each stem segment explant could induce 1-3 adventitious buds;

...

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Abstract

The invention relates to a quick adventitious buds inducing method for the panax tuber section. The method uses the panax tuber section as the explants, and induces the adventitious buds on the MS substrate of the additional hormone, and to proceed with the multiplication of the adventitious buds. The invention can directly induce the buds through the path produced by the direct organs, the speedis high, the sprouting rate is high, the cost is low, and the inducing process of the adventitious buds can be completed after 25 days about, the reproducing frequency is high, the variation rate is low, the period is relatively short, and the un-differentiated cells can directly differentiate into buds, so that the clone variation of the body cells is small. The invention can better keep the genetic stability of the host plant, and can ensure the transformed exogenous gene to transmit stably, the limit from the genotype is low, the adventitious buds obtaining rate is relatively high, and thereby is an effective measurement for obtaining the artificially cultivated panax seedlings in large scale.

Description

technical field [0001] The invention relates to a method for rapidly inducing adventitious buds in ginseng tissue culture, in particular to a method for rapidly obtaining ginseng adventitious buds by using the stem section of aseptic ginseng seedlings as explants and culturing them on a culture medium. Background technique [0002] Ginseng (Panax ginseng C.A.Mey.) is a perennial herb of Araliaceae. Treatment of physical weakness, cold limbs, weak pulse, spleen deficiency, lack of food, lung deficiency, panting and coughing, thirsty wounds, internal heat and thirst, chronic illness and weakness, palpitations and insomnia, impotence, cold uterus, heart failure, cardiogenic shock, etc. At the same time, the application of ginseng in health food and health cosmetics is more and more extensive. [0003] Ginseng likes a cool and humid climate, and it mostly grows in coniferous and broad-leaved mixed forests or miscellaneous forests on gentle slopes or slopes of mountains at an al...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 赵民安曹玉锦王晓军
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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