Method for inducing regeneration of adventitious bud of western balsam poplar by histone deacetylase inhibitor

A technology of acetylase inhibitors and sirtuins, which is applied in the field of histone deacetylase inhibitors to induce adventitious bud regeneration of Populus trichocarpa, can solve the problems of long time for adventitious buds and low induction rate of adventitious buds, and achieve The effect of shortening the induction period and simplifying the tissue culture system

Inactive Publication Date: 2013-12-25
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the current young leaves and stems of Populus trichocarpa are used as explants to induce adventitious buds, and the time-consuming problem of using callus to induce adventitious buds is long.

Method used

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  • Method for inducing regeneration of adventitious bud of western balsam poplar by histone deacetylase inhibitor
  • Method for inducing regeneration of adventitious bud of western balsam poplar by histone deacetylase inhibitor
  • Method for inducing regeneration of adventitious bud of western balsam poplar by histone deacetylase inhibitor

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specific Embodiment approach 1

[0011] Specific embodiment one: the method that a kind of histone deacetylase inhibitor of the present embodiment induces the adventitious bud regeneration of Populus trichocarpa is carried out according to the following steps: one, get the stem section of Populus trichocarpa containing axillary bud in WPM medium First, induce axillary buds to germinate until seedlings grow; second, culture the Populus trichocarpa seedlings in step 1 in WPM medium until adventitious roots grow; third, put the seedlings that grow adventitious roots in step 2 into a medium containing 0-5 μM histone The aqueous solution of the sirtuin inhibitor is continuously cultivated until the regeneration of adventitious buds is induced, that is, the regeneration of the adventitious buds induced by the histone sirtuin inhibitor is completed.

[0012] This embodiment includes the following beneficial effects:

[0013] At present, the research on plant HDAC mainly involves the growth rate of roots, the formati...

specific Embodiment approach 2

[0014] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the induction conditions for inducing the germination of axillary buds described in step 1 are: the temperature is 23-25°C, and the light time is 16 hours / day. The seedlings are induced until the seedlings grow to a height of 2-3 cm. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0015] Specific embodiment 3: The difference between this embodiment and specific embodiment 1 or 2 is that the culture conditions described in step 2 are: cultivating at a temperature of 23-25°C and a light time of 16 hours / day until long Adventitious root length is 0.5 ~ 1.5cm. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses a method for inducing the regeneration of an adventitious bud of western balsam poplar by a histone deacetylase inhibitor. The method aims to solve conventional problems that the success rate of inducing the adventitious bud by taking a western balsam poplar tissue cultured seedling as an explant is low, and the period of inducing the adventitious bud by a callus is long. The method is characterized in that 0 to 5 microns of a histone deacetylase inhibitor solution is added into a culture medium for the tissue cultured seedling to induce the regeneration of the adventitious bud of the western balsam poplar. The method has the following advantages that (1) the method is simple, convenient and effective, complex tissue culture systems and culture conditions are not required, and the regeneration of the adventitious bud can be induced by only adding the histone deacetylase inhibitor solution with proper concentration into a common poplar tissue culture medium; (2) the period is short, and the adventitious bud can be induced usually after about 30 days.

Description

technical field [0001] The invention relates to a method for inducing regeneration of adventitious buds of Populus trichocarpa with histone deacetylase inhibitors. Background technique [0002] Epigenetic regulation mainly includes DNA methylation and histone modification, which in turn includes histone acetylation, phosphorylation, and glycosylation. The level of histone acetylation is jointly regulated by histone acetylation mediated by histone acetyltransferase (HAT) and histone deacetylation mediated by histone deacetylases (HDACs) , one is indispensable, and the two work together to ensure that the level of histone acetylation in the cell is in a dynamic balance. Histone acetylation affects the structure of chromatin, gene transcription, and various life activities. HDAC catalyzes the removal of acetyl groups bound to lysine on histones. After removal of acetyl groups, the chromatin structure becomes tighter, which is not conducive to the binding of transcription fact...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 马旭俊李淑娟杨传平
Owner NORTHEAST FORESTRY UNIVERSITY
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