92results about How to "High regeneration frequency" patented technology

The invention discloses a relay-intercropping cultivation method of polygonatum sibiricum. The relay-intercropping cultivation method is characterized in that an uncaria relay-intercropping mode is adopted and imitated wild plantation is performed at borderlands of mountain forests. The cultivation method comprises following steps of: firstly, selecting moisture and completely-shaded land parcels; secondly, performing fixed planting operation on uncaria seedlings in accordance with 1.0m*1.5m distance between plants; thirdly, making a compartment surface, 50cm wide and 30cm high, between lines of uncarias; fourthly, planting two rows of polygonatum sibiricum on compartment surfaces in the shapes of regular triangles or inverted triangles; fifthly, managing uncaria and polygonatum sibiricum daily and preventing and controlling plant diseases and insect pests; and lastly, timely harvesting and processing polygonatum sibiricum.The relay-intercropping cultivation method of polygonatum sibiricum has following beneficial effects: a shed may not be required for shading since a growth condition is provided for imitated wild growth of polygonatum sibiricum, thereby solving the problem that manually-planted medicinal materials have poor quality; and high yield of polygonatum sibiricum is realized while an additional amount of uncaria is harvested such that land resources are fully utilized and comprehensive income is increased.

The invention mainly relates to a method for regenerating a plant from camellia callus. The method has the following steps: stripping off the inner and outer seed coats of a camellia fruit seed, and inoculating the seed to a 1/2MS culture medium; when a sterile seedling grows above 3 cm, inoculating the soft leaf of the young plant to a callus induction culture medium which is MS + 0.5mg.L 6-BA+1.0mg.L 2,4-D; when the callus grows to get a diameter about 1 cm, shifting the callus to a callus differentiation culture medium which is MS+mg.L 6-BA20+0.1mg.L I BA+ mg.L KT0.1; when an indefinite bud grows to 0.5 cm, carrying out the separation and inoculating to a strong bud culture medium which is MS + 0.2mg.L 6-BA+0.05mg.L NAA; and when a bud stick grows to 4 to 5 cm, cutting off the basal of the bud stick, immersing the basal of the bud stick in 1,000 g.L I BA, and then inoculating to a MW + 0.2mg.L I BA+0.2mg.L NAA culture medium. The method has the advantages that: the method has a simple culture medium recipe, a simple and convenient operating process, short culture time, high regeneration frequency and a high propagation expansion coefficient, and facilitates the large-scale production of rare camellia plants and the realization of the genetic transformation of exogenous genes.

The invention discloses a micropropagation method of fraxinus rhynchophylla and relates to the micropropagation method. The problems of long reproductive cycle, low reproduction efficiency and poor offspring genetic stability in nursery stock propagation of fraxinus rhynchophylla are solved. The method comprises the following steps of: 1, sterilizing fraxinus rhynchophylla seeds, and culturing single cotyledons of zygotic embryos to obtain cotyledonal cell embryos; 2, performing maturation cultivation on the cotyledonal cell embryos; 3, performing sprouting cultivation on the cotyledonal cellembryos subjected to maturation cultivation to obtain regenerated plants; and 4, transplanting the regenerated plants into a culture medium to culture until new leaves are completely unfolded, and removing a covered plastic thin film. The micropropagation method has the advantages of short nursery stock reproductive cycle, high reproductive rate (culturing for 60 to 70 days) and high reproductionefficiency (each explant can generate 15 to 20 somatic embryos); the germination rate of the somatic embryos is up to 87 to 89.55 percent; the transplanting survival rate of the regenerated plants isup to 75 to 80 percent; and the regeneration plants with the same good characteristics as female parents can be generated in mass.

The invention discloses a method for wheat tissue culture, which comprises the following steps: firstly, performing induction culture on a wheat germ to obtain an embryogenic callus; and secondly, performing differentiation culture on the embryogenic callus to obtain a wheat regenerated plantlet, wherein the method of the induction culture comprises the steps of successively performing induction cultures on the wheat germ with a culture medium containing auxin, a culture medium without the auxin, and the culture medium containing the auxin again to obtain the embryogenic callus. The method for the wheat tissue culture further improves the regeneration frequency of immature embryos and mature embryos of wheat, weakens the barriers caused by the conditions such as genotypes, physiological states and the like to the regeneration, provides a technical support for the cell engineering breeding, the genetic engineering breeding and the functional genomics research of the wheat, and has great significance for the biotechnology breeding and the functional genomics research of the wheat.

The invention discloses a plant binary expression vector for inducing embryoid body formation and plant regeneration, which comprises BBM genes and an FLP-FRT site specific recombination system. The FLP-FRT site specific recombination system comprises FLP genes and two identical orientation FRT sites. The BBM genes and the FLP genes are positioned between the two identical orientation FRT sites, the BBM genes are controlled by a constitutive promoter, and the FLP genes are controlled by an inducible promoter. The invention further discloses a method for constructing the plant binary expression vector and a method for inducing embryoid body formation and plant regeneration by using the plant binary expression vector. The invention can not only promote embryoid body formation of a plant callus, induce formation of a transgenic plant, and remarkably improve the transformation frequency and the regeneration frequency of the plant; but also can eliminate the negative impacts of exogenous genes on the transgenic plant. The invention provides a novel genetic transformation strategy for plant species which are difficult for genetic transformation, and has wide application prospect.

The present invention discloses the in vitro propagation process of switchgrass. The in vitro propagation process includes the following steps: cutting 3-4 middle stem nodes after switchgrass grows to possess 5-6 stem nodes, and artificially inducing regeneration bud in MBP culture medium; excising the growing point of cespitose bud repeatedly for 4 or 5 times until obtaining cespitose bud tuber with diameter of about 0.5 cm, and inducing in new MBP culture medium to obtain large cespitose bud; culturing the regenerated plant in rooting culture medium SMO to obtain seedling; and transplanting to greenhouse or field. The in vitro propagation process of switchgrass has expanded tissue culture and genetic transformation range of switchgrass, shorted culture period, raised regeneration frequency and lowered experiment cost. The present invention provides effective measure for the fast propagation of transgenic switchgrass.

The invention relates to the technical field of plant tissue culture, in particular relates to a potato stem fragment tissue culture medium, and a one-step seedling culture method for potato test-tube plantlet stem fragments. An MS traditional culture medium is selected to provide inorganic nutritional elements and partial organic nutritional elements required by tissue growth and development. The culture medium provided by the invention has a broad spectrum, and is suitable for one-step seedling culture for stem tissues of most potato varieties. Through the reasonable control of the use and compounding ratio of hormones and the addition of other auxiliary materials, the stem fragment explant does not need to be transferred to a differential culture medium after forming callus tissues, instead, is subjected to direct differentiation and seedling in an original culture medium, so as to shorten the culture time by about two months and reduce the economic cost. In addition, the culture medium has a broad spectrum, and is suitable for most varieties.

The invention relates to a quick adventitious buds inducing method for the panax tuber section. The method uses the panax tuber section as the explants, and induces the adventitious buds on the MS substrate of the additional hormone, and to proceed with the multiplication of the adventitious buds. The invention can directly induce the buds through the path produced by the direct organs, the speed is high, the sprouting rate is high, the cost is low, and the inducing process of the adventitious buds can be completed after 25 days about, the reproducing frequency is high, the variation rate is low, the period is relatively short, and the un-differentiated cells can directly differentiate into buds, so that the clone variation of the body cells is small. The invention can better keep the genetic stability of the host plant, and can ensure the transformed exogenous gene to transmit stably, the limit from the genotype is low, the adventitious buds obtaining rate is relatively high, and thereby is an effective measurement for obtaining the artificially cultivated panax seedlings in large scale.

Methods for transforming plants, particularly commercially important elite maize inbreds, are provided. The methods involve transformation of meristematic organogenic tissue or immature embryos, and include the use of defined plant growth media. The methods disclosed provide more stable transgenic plants, and permit the transformation of varieties of cereals that are not amenable to transformation by conventional approaches.

The invention discloses a method for rapidly propagating wild ginseng. The method mainly comprises the following steps of: inducing callus, differentiating buds, performing successive transfer culture, performing multiplication culture, performing rooting culture, and hardening seedlings and transplanting. Good effects of high propagation speed, stable hereditary character, high propagation coefficient and eugenic test-tube seedlings are achieved; and through culture, the germination rate of the callus of the wild ginseng is 95 percent, the proliferation times is 4.8 times, the rooting percentage is 96 percent, the final survival rate is 98 percent and the quality is well ensured.

The invention discloses a method for improving a tissue culture regeneration rate of a wheat genotype immature embryo with low regeneration capacity, which comprises the following steps that 1) an immature embryo of a wheat variety with high regeneration capacity and an immature embryo of a wheat variety with low regeneration capacity are inoculated inside the same container with a callus induction culture medium at line intervals to be cultured in an induction way under a darkness condition, so that a callus of the wheat variety with the high regeneration capacity and a callus of the wheat variety with the low regeneration capacity are obtained; and 2) the callus of the wheat variety with the high regeneration capacity and the callus of the wheat variety with the low regeneration capacity are transferred into the same culture container with a differential culture medium at line intervals to be differentially cultured under the illumination condition, so that a regeneration plant of the wheat variety with the low regeneration capacity and a regeneration plant of the wheat variety with the high regeneration capacity are obtained. Due to the adoption of the method, a regeneration rate, an embryo callus induction rate and a callus differentiation rate of the plants cultured by the immature embryos of the wheat variety with the low regeneration capacity can be remarkably improved.

The invention discloses a method for inducing the regeneration of an adventitious bud of western balsam poplar by a histone deacetylase inhibitor. The method aims to solve conventional problems that the success rate of inducing the adventitious bud by taking a western balsam poplar tissue cultured seedling as an explant is low, and the period of inducing the adventitious bud by a callus is long. The method is characterized in that 0 to 5 microns of a histone deacetylase inhibitor solution is added into a culture medium for the tissue cultured seedling to induce the regeneration of the adventitious bud of the western balsam poplar. The method has the following advantages that (1) the method is simple, convenient and effective, complex tissue culture systems and culture conditions are not required, and the regeneration of the adventitious bud can be induced by only adding the histone deacetylase inhibitor solution with proper concentration into a common poplar tissue culture medium; (2) the period is short, and the adventitious bud can be induced usually after about 30 days.

An application of the plant growth regulator TDZ (N-phenyl-N'-1,2,3-thiadiazine- 5-urea) in fast reproduction of Tianshan mountain snow lotus and control its morphogenesis is disclosed.

The invention discloses a rapid propagation method for dysosma versipellis. The rapid propagation method includes the following steps that 1, sterile dysosma versipellis explants are manufactured; 2, the sterile explants are cultured through an induction medium to obtain embryonic callus; 3, the embryonic callus is cultured through a first multiplication medium, and the embryonic callus is subcultured for 2-3 generations to obtain subcultured embryonic callus; 4, the subcultured embryonic callus is cultured through a differentiation medium to obtain somatic embryos; 5, the somatic embryos are cultured through a second multiplication medium to obtain subcultured somatic embryos; 6, the subcultured somatic embryos are cultured through a plant regeneration medium to obtain dysosma versipellis plants. According to the rapid propagation method, the obtained dysosma versipellis embryogenic cell can induce regeneration of the dysosma versipellis plants, and the dysosma versipellis plants have the advantages of being high in growth rate and plant regeneration frequency, stable in character and the like.

A method for the tissue culture and transplanting of cucumber includes such steps as taking the aseptic seedling of cucumber, choosing and inoculating explant, inducing regeneration bud, differentiating, growing, rooting culture, pratisizing seedlings and transplanting.

The invention provides a method for culturing a male sterile tobacco haplont via an unfertilized ovule. According to the method for culturing the male sterile tobacco haplont, an ovule of which the development of an unpollinated male sterile tobacco bud is between a megasporocyte stage and a binucleate stage is used as a culture medium, after sterilization treatment, the ovule is sequentially subjected to embryoid induced culture, regeneration bud rooting culture and regenerated plant culture in a 28-DEG C illumination culture chamber, so that the male sterile tobacco haplont is finally cultured, and during the period of the culture, the illumination continuously lasts for 10 hours every day under a 1,500 Lux illumination condition. The method for culturing the male sterile tobacco haplont via the unfertilized ovule is adopted to culture male sterile tobaccos, so that the frequency of tobacco ovule embryoid induction and plant regeneration are remarkably improved.

The invention discloses a regenerating method of a catalytic conversion catalyst. The regenerating method comprises the following steps: a catalyst which is transferred from a reactor firstly enters a first generator to be blown and regenerated by first regenerated gas; and a primary regenerant at the outlet of the first generator is conveyed to a catalyst flow distributor and is divided into two material flows which respectively enters a second regenerator and the reactor, wherein the flow of the primary regenerant flow entering the reactor accounts for 1-100% of the total flow of the primary regenerant in the flow and a part of primary regenerant enters a second regenerator to be secondarily regenerated by virtue of second regenerated gas to obtain a secondary regenerant which is combined with the primary regenerant flow and enters the reactor together. According to the regeneration method disclosed by the invention, the capacity of the existing reactor can be effectively improved, frequent charking regeneration of the catalyst is avoided and the regenerating temperature and temperature rise are reduced, and the total service life of the catalyst is prolonged. Moreover, the flow rate of the catalyst in different mobile bed reactors is independently regulated, and the regenerating method can be used in industrial production of methanol to propylene.

The invention provides a Y-shaped SCR system catalyst online continuous regenerating device. The Y-shaped SCR system catalyst online continuous regenerating device comprises one first SCR reactor and one second SCR reactor, one smoke inlet pipeline, one flue switching structure provided with one first station and one second station, a first catalyst regeneration system connected with the first SCR reactor and a second catalyst regeneration system connected with the second SCR reactor, wherein the smoke inlet pipeline is communicated with the first SCR reactor when the flue switching structure is at the first station, and the smoke inlet pipeline is communicated with the second SCR reactor when the flue switching structure is at the second station. The Y-shaped SCR system catalyst online continuous regenerating device provided by the invention has the advantages that online regeneration of a denitration catalyst can be realized without halting a machine set, forced halt of the machine set due to SCR catalyst activity decrease is reduced, and economic security and operation level of the machine set are improved, so that operation cost of an SCR system is saved.

The invention discloses a cucumber high frequency plant generative method, composed of exophytic preparation of Flamingo-bill, inducement and increasement of indefinite buds, radication and transplantation and the like. According to the invention, only one phytohormone is used during the process of tissue culture, density is lower, clone generation of somatic cells are not easy to be induced, technical cost is saved; technical link is few, operation is simple, producing efficiency is increased; only 45 d are required from inoculability to formation of the full plant, thereby greatly shortening generative time of the plant.

The invention discloses a method for inducing cluster bud multiplication and plant regeneration of asparagus cochinchinensis. Stems, with axillary buds, of aseptic germinated seedlings of seeds are used as explants for axillary bud induction, cluster bud multiplication, rooting culture and acclimatization and transplantation to obtain plant regeneration of the asparagus cochinchinensis. By the adoption of the method, the axillary bud induction rate can reach 85.05%, the cluster bud multiplication multiple reaches 3.65, the rooting rate reaches 78.27%, the transplanting survival rate reaches 87.10%, the production cost can be effectively reduced, the tissue culture and rapid propagation period can be effectively shortened, and the method can be applied to large-scale seedling propagation and resource protection of the asparagus cochinchinensis.