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Method for obtaining haploid regenerated plant by chilli anther culturing

A technology for regenerating plants and pepper anthers, applied in the agricultural field, can solve the problems of low pepper anther culture efficiency and the like, and achieve the effects of reducing callus browning, high seedling emergence rate, and increased frequency

Inactive Publication Date: 2014-09-17
NANJING INST OF VEGETABLE SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for obtaining haploid regenerated plants by culturing pepper anthers for the low efficiency of pepper anther culture proposed in the background technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0040] In this example, the first-generation hybrid capsicum combinations P1302 and P1212 with comprehensive and excellent traits were used as test materials, and haploid plants were obtained by the method of the present invention. The implementation process is as follows:

[0041] 1. Materials

[0042] In early May, when the test materials are in full bloom, flower buds were collected at 10:00 am on a sunny day. Carmine acetic acid dyed and pressed slices were used to examine the anther development period under the microscope, and the flower buds in the middle and late stages of mononuclei, with a bud length of 5.5-6.5 mm and a corolla slightly longer than the calyx were selected; under suitable growth environment conditions, flower buds were differentiated 23-25 ​​days after flowering, that is, 6 days before flowering. ~8d flower buds.

[0043] 2. Disinfection

[0044] (1) Sterilization and packaging of medium: sterilize 60mm Petri dishes, Erlenmeyer flasks, solid medium,...

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Abstract

The invention relates to a method for obtaining a haploid plant by chilli anther culturing, which belongs to the technical field of agriculture. The method comprises the steps of sterilizing selected flower bud, grafting anther, pretreating, culturing anther, and inducing callus tissue to be subjected to redifferentiation to form the haploid plant. According to the method, a microspore and an anther wall are co-cultured by virtue of a solid culturing medium, low temperature and heat shock pretreatment is performed, and callus induction, culturing medium component differentiation and culturing conditions are adjusted, so that the callus rate and the differentiating capability of the callus tissue are enhanced. When the method is used for culturing chilli anther, the callus inductivity reaches over 60%, the emergence rate reaches over 80% and the problem of low inductivity in chilli anther culturing is solved. By virtue of the method, the haploid regenerated plant can be obtained rapidly and the emergence rate is high; a powerful approach is provided for new variety breeding.

Description

Technical field: [0001] The invention belongs to the field of agriculture, and in particular relates to a method for obtaining haploid regenerated plants by culturing capsicum anthers. technical background: [0002] Capsicum is one of the important vegetable crops, and its cultivation is common and extensive. The heterosis of pepper is very significant. At present, conventional breeding methods are mostly used to select new varieties. The breeding cycle is long and the progress is slow. The unused pepper germplasm materials are decreasing day by day, and the germplasm resources are scarce. [0003] Anther culture is an effective method to obtain pure lines at the single-cell level and is an important auxiliary means of breeding. Anther culture can quickly fix and preserve favorable genes, which can be used for the cultivation and stabilization of new types of distant hybrids, as well as selection of specific traits, as the basic material for breeding; anther culture can qui...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张燕燕黄忠阳魏猷刚唐懋华缪其松刘叶琼胡静孙雪花
Owner NANJING INST OF VEGETABLE SCI
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