Micropropagation method of fraxinus rhynchophylla

A micropropagation technology of Astragalus chinensis, applied in the field of micropropagation, can solve the problems of long reproduction cycle, low reproduction efficiency, and poor genetic stability of offspring, etc., and achieve the effects of short reproduction cycle, high reproduction efficiency and fast reproduction speed

Inactive Publication Date: 2011-11-02
NORTHEAST FORESTRY UNIVERSITY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a micropropagation method for Astragalus in order to solve the problems of long propagation period, low multiplication efficiency and poor genetic stability of offspring in the propagation of Astragalus seedlings

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  • Micropropagation method of fraxinus rhynchophylla

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specific Embodiment approach 1

[0016] Specific embodiment one: the micropropagation method of the present embodiment is realized by the following steps: 1. Get the seeds of Astragalus and carry out disinfection treatment, strip off the zygotic embryo, cut the single cotyledon of the zygotic embryo and inoculate it into a seedling containing 0.25~0.75 mg / L cytokinin, 0.5-2.0 mg / L auxin, 100-700 mg / L acid hydrolyzed casein, 30-100 g / L sucrose and 5-7 g / L agar induction medium, at a temperature of 20 Cultivate for 2-4 weeks at ~30°C, humidity 40%-70%, and no light to obtain cotyledon-shaped somatic embryos;

[0017] 2. Inoculate the cotyledon-shaped somatic embryos on the mature medium containing 30-100g / L sucrose and 5-7g / L agar, at a temperature of 20-30°C, a humidity of 40%-70%, and a light intensity of 20-30%. 60μmol·m -2 ·s -1 , Cultivate under the condition of 16-20 hours of light every day for 3-5 weeks;

[0018] 3. Inoculate the mature cultured cotyledon-shaped somatic embryos on the germination med...

specific Embodiment approach 2

[0034] Specific embodiment two: The difference between this embodiment and specific embodiment one is that the process of disinfection treatment in step one is: peel off the pericarp coated on the outside of the seeds, rinse the seeds with running water for 20-40min, and use a volume concentration of 70%-80%. Soak the seeds in alcohol for 30-120s, then soak the seeds in NaClO with a volume concentration of 2%-6% for 10-20min to sterilize the body surface, and rinse the soaked seeds with sterile water for 3-7 times. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0035] Embodiment 3: The difference between this embodiment and Embodiment 1 is that in step 1, the temperature is 25° C., the humidity is 50%, and the condition of no light is cultivated for 2 weeks to obtain cotyledon-shaped somatic embryos. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention discloses a micropropagation method of fraxinus rhynchophylla and relates to the micropropagation method. The problems of long reproductive cycle, low reproduction efficiency and poor offspring genetic stability in nursery stock propagation of fraxinus rhynchophylla are solved. The method comprises the following steps of: 1, sterilizing fraxinus rhynchophylla seeds, and culturing single cotyledons of zygotic embryos to obtain cotyledonal cell embryos; 2, performing maturation cultivation on the cotyledonal cell embryos; 3, performing sprouting cultivation on the cotyledonal cellembryos subjected to maturation cultivation to obtain regenerated plants; and 4, transplanting the regenerated plants into a culture medium to culture until new leaves are completely unfolded, and removing a covered plastic thin film. The micropropagation method has the advantages of short nursery stock reproductive cycle, high reproductive rate (culturing for 60 to 70 days) and high reproductionefficiency (each explant can generate 15 to 20 somatic embryos); the germination rate of the somatic embryos is up to 87 to 89.55 percent; the transplanting survival rate of the regenerated plants isup to 75 to 80 percent; and the regeneration plants with the same good characteristics as female parents can be generated in mass.

Description

technical field [0001] The invention relates to a micropropagation method. Background technique [0002] Fraxinus rhynchophylla is a large deciduous tree of Oleaceae Oleaceae, and it is an important economic hard broad tree species in the eastern mountains of Northeast my country. Astragalus has strong stress resistance and strong ornamental value. The wood and various products of Aspergillus japonica are in short supply in the international and domestic markets, the price is expensive, and they have high economic value. [0003] Willows are propagated by seeds. At present, there is no mother forest and seed orchard in our country, and the existing mother trees of Astragalus are all wild, and the number is not large. In production and scientific research, the seedlings of Aspergillus fruticosa are cultivated by sowing and propagating. There are multiple disadvantages in the mode of sowing and reproduction: 1, the growth cycle of seedlings is long, and the molding is slow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨玲沈海龙单琳
Owner NORTHEAST FORESTRY UNIVERSITY
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