Method for quickly propagating seedlings of Dendrobium spp. by tissue culture
A tissue culture and dendrobium technology, applied in the field of plant tissue culture, can solve problems such as unsatisfactory proliferation efficiency and transplanting survival rate, unfavorable high-quality autumn dendrobium orchid seedlings, complicated operation process, etc. The effect of reducing culture cost and high induction rate
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Embodiment 1
[0044] Selection and disinfection of explants: Select the healthy mother plant of Dendrobium orchid 'Sanya Sunshine' (introduced in Hainan) as the object of explants, and when the new buds germinate 1.0-2.0cm, use sterile fresh-keeping bags for bagging and isolation Take samples when the buds have 3 to 5 nodes, remove the leaves, rinse with tap water, then soak in 75% alcohol on the ultra-clean workbench for 30s, and then transfer to 0.1% mercuric chloride solution for shaking Disinfect for 5 minutes, then replace the 0.1% mercuric chloride solution once, then sterilize with shaking table for 5 minutes, the speed of shaking table oscillation is 90r / min; finally take it out on the ultra-clean workbench and rinse it with sterile water for 4 times, and then use sterile Dry the water with bacterial paper towels, cut off the segmented stems and stem tips, and set aside;
[0045] Induction culture of cluster buds: get the resulting jointed stem segment and shoot apex, and inoculate ...
Embodiment 2
[0050] Selection and disinfection of explants: select the healthy mother plant of Dendrobium orchid 'Crystal' (introduced in Hainan) as the object of explants, and when the new buds germinate 1.0-2.0 cm, use sterile fresh-keeping bags to carry out bagging and isolation; Take samples when the sprouts have 3 to 5 nodes, remove the leaves, rinse them with tap water, then soak them in 75% alcohol on the ultra-clean workbench for 50 seconds, and then transfer them to 0.1% mercuric chloride solution for shaking disinfection 6min, then replace the 0.1% mercuric chloride solution for 1 time, and then shake the table for 5 minutes to sterilize. Blot the water with a paper towel, cut off the section of the stem with nodes and the tip of the stem, and set aside;
[0051] Induction culture of cluster buds: get the resulting jointed stem segment and shoot apex, and inoculate them into the cluster bud induction medium for cluster bud induction culture. The formula of the cluster bud inducti...
Embodiment 3
[0056]Selection and disinfection of explants: Select the healthy mother plant of Dendrobium orchid 'Pink Lady' (introduced in Hainan) as the object of explants, and when the new buds germinate 1.0-2.0cm, use sterile fresh-keeping bags for bagging and isolation ; Take samples when the sprouts have 3 to 5 nodes, remove the leaves, rinse with tap water, then soak in 75% alcohol for 42s on the ultra-clean workbench, and then transfer to 0.1% mercuric chloride solution for shaking Disinfect for 6 minutes, then replace the 0.1% mercuric chloride solution once, then sterilize with shaking table for 5 minutes, the speed of shaking table shaking is 90r / min; finally take it out on the ultra-clean workbench and rinse it with sterile water for 5 times, and then use sterile Dry the water with bacterial paper towels, cut off the segmented stems and stem tips, and set aside;
[0057] Induction culture of cluster buds: get the resulting jointed stem segment and shoot apex, and inoculate them ...
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