Method for quickly propagating seedlings of Dendrobium spp. by tissue culture

A tissue culture and dendrobium technology, applied in the field of plant tissue culture, can solve problems such as unsatisfactory proliferation efficiency and transplanting survival rate, unfavorable high-quality autumn dendrobium orchid seedlings, complicated operation process, etc. The effect of reducing culture cost and high induction rate

Active Publication Date: 2017-08-18
CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The above documents all provide the protocorm induction pathway propagation technology of autumn Dendrobium orchid, but the protocorm induction pathway has to go through the process of dedifferentiation and re-differentiation culture, which takes a long time and has a low seedling rate, which is very important for the acquisition of high-quality seedlings of Dendrobium orchid. unfavorable
[0008] In order to solve the problems existing in the protocorm induction pathway, the application number is CN200410077729.5, and in the Chinese patent titled "Method for Tissue Culture and Rapid Propagation of Autumn Dendrobium High-quality Seedlings", it is proposed to use the autumn Dendrobium germplasm sprouts as explants , using culture medium to proliferate clustered buds and obtain seedlings; although it has established the tissue culture propagation technology of Dendrobium orchid, the proliferation efficiency and transplanting survival rate are still not ideal, and there is a defect that the operation process is relatively complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Selection and disinfection of explants: Select the healthy mother plant of Dendrobium orchid 'Sanya Sunshine' (introduced in Hainan) as the object of explants, and when the new buds germinate 1.0-2.0cm, use sterile fresh-keeping bags for bagging and isolation Take samples when the buds have 3 to 5 nodes, remove the leaves, rinse with tap water, then soak in 75% alcohol on the ultra-clean workbench for 30s, and then transfer to 0.1% mercuric chloride solution for shaking Disinfect for 5 minutes, then replace the 0.1% mercuric chloride solution once, then sterilize with shaking table for 5 minutes, the speed of shaking table oscillation is 90r / min; finally take it out on the ultra-clean workbench and rinse it with sterile water for 4 times, and then use sterile Dry the water with bacterial paper towels, cut off the segmented stems and stem tips, and set aside;

[0045] Induction culture of cluster buds: get the resulting jointed stem segment and shoot apex, and inoculate ...

Embodiment 2

[0050] Selection and disinfection of explants: select the healthy mother plant of Dendrobium orchid 'Crystal' (introduced in Hainan) as the object of explants, and when the new buds germinate 1.0-2.0 cm, use sterile fresh-keeping bags to carry out bagging and isolation; Take samples when the sprouts have 3 to 5 nodes, remove the leaves, rinse them with tap water, then soak them in 75% alcohol on the ultra-clean workbench for 50 seconds, and then transfer them to 0.1% mercuric chloride solution for shaking disinfection 6min, then replace the 0.1% mercuric chloride solution for 1 time, and then shake the table for 5 minutes to sterilize. Blot the water with a paper towel, cut off the section of the stem with nodes and the tip of the stem, and set aside;

[0051] Induction culture of cluster buds: get the resulting jointed stem segment and shoot apex, and inoculate them into the cluster bud induction medium for cluster bud induction culture. The formula of the cluster bud inducti...

Embodiment 3

[0056]Selection and disinfection of explants: Select the healthy mother plant of Dendrobium orchid 'Pink Lady' (introduced in Hainan) as the object of explants, and when the new buds germinate 1.0-2.0cm, use sterile fresh-keeping bags for bagging and isolation ; Take samples when the sprouts have 3 to 5 nodes, remove the leaves, rinse with tap water, then soak in 75% alcohol for 42s on the ultra-clean workbench, and then transfer to 0.1% mercuric chloride solution for shaking Disinfect for 6 minutes, then replace the 0.1% mercuric chloride solution once, then sterilize with shaking table for 5 minutes, the speed of shaking table shaking is 90r / min; finally take it out on the ultra-clean workbench and rinse it with sterile water for 5 times, and then use sterile Dry the water with bacterial paper towels, cut off the segmented stems and stem tips, and set aside;

[0057] Induction culture of cluster buds: get the resulting jointed stem segment and shoot apex, and inoculate them ...

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PUM

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Abstract

The invention provides a method for quickly propagating seedlings of Dendrobium spp. by tissue culture. The method uses new buds of Dendrobium spp. as explants to arrive at quick propagation by means of induction of cluster buds and growth of buds from buds. The method comprises the steps of selecting explants, sterilizing the explants, subjecting cluster buds to induction culture, subjecting the cluster buds to enrichment culture, performing rooting culture, and transplanting test tube seedlings. The seedlings of Dendrobium spp. can be acquired by using the method just in 5-6 months; induction rate in the propagation process is high, subsequent rooting culture after the propagation can be performed without strong seedling culture, the culture links are simplified, culture cost is reduced, multiplication efficiency is high, and the efficiency of the whole seedling culture is improved; in addition, the test tube seedlings cultured herein grow robust with developed roots, and may gain high environmental adaptability through acclimatization, so that seedling transplanting survival rate is increased.

Description

[0001] 【Technical field】 [0002] The invention belongs to the technical field of plant tissue culture, and in particular relates to a tissue culture rapid propagation method for dendrobium orchid seedlings. [0003] 【Background technique】 [0004] Autumn Dendrobium orchid (Dendrobium spp.) is an evergreen species of Dendrobium orchidaceae, also known as butterfly Dendrobium, Duran, Dendrobium, Dendrobium, etc. Most of them are hybrid varieties bred from the parent of the tropical native species Dendrobium Phalaenopsis native to New Guinea. Autumn Dendrobium is a popular flower in the Chinese flower arrangement market in recent years. It has a wide variety, beautiful flower shape, bright colors, long flowering period, and high ornamental value. The domestic market demand for cut flowers and potted flowers is increasing year by year. There is an urgent need for high-quality seedlings. [0005] In recent years, many varieties of autumn Dendrobium orchids have been introduced in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 叶秀仙黄敏玲钟淮钦樊荣辉林榕燕
Owner CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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