Culture medium for culturing urine-derived cells
A culture medium and basal medium technology, applied in the direction of cell culture medium, culture process, tissue culture, etc., can solve the problems of death, prone to apoptosis, difficult logarithmic growth phase, etc., and achieve the effect of promoting cell culture
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Embodiment 1
[0093] According to Table 1, the different groups of culture media were mixed, and the components other than the basic medium were added first, and then the basic medium was added to make up. Among them, the basic medium included DMEM, DMEM / F12, αMEM, RPMI 1640 , CMRL-1066, Ham's F12, IMDM, 199, MCDB.
[0094] Table 1 Cell culture medium formula without xenogeneic urine source
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[0098] Digest primary urine-derived cells and count them. Plate 1500 cells per well (96-well plate). Day0 uses primary urine cell culture medium (REGM medium) for culture, and Day1, Day3, and Day5 are replaced with corresponding groups The medium was cultured, 150 μl of medium per well, and MTT detection was performed on Day1, Day4, and Day7.
[0099] After MTT test, the MTT results of groups 1-25 are as follows: figure 1 shown.
[0100] figure 1 It is the result picture of MTT. The principle of MTT detection is that succinate dehydrogenase in the mitochondria of...
Embodiment 2
[0103] In this example, on the basis of Group 1 in Example 1, the medium was formulated according to Table 2, wherein the volume ratio of platelet lysate to the medium was added according to Table 3 to obtain different groups of medium. When configuring, first add the components other than the basal medium, and then add the basal medium to supplement, wherein the basal medium is DMEM / F12:DMEM=1:1.
[0104] Table 2 Components of culture medium containing platelet lysates in different volume ratios
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[0106] Table 3 Volume ratio of platelet lysate
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[0109] Digest primary urine-derived cells and count them. Plate 1500 cells per well (96-well plate). Day0 uses primary urine cell culture medium (REGM medium) for culture, and Day1, Day3, and Day5 are replaced with corresponding groups The culture medium was cultured, and the medium volume of each well was 150 μl, and MTT detection was performed on Day1, Day4, and Day7.
[0110] After the MTT test...
Embodiment 3
[0113] According to Table 4, different groups of culture media were mixed, and the components other than the basal medium were added first, and then the basal medium was added for supplementation, wherein the basal medium was DMEM / F12:DMEM=1:1.
[0114] Platelet lysate and various vitamin Cs were added to the medium formula in Table 4, vitamin C including L-ascorbic acid, vitamin C phosphate magnesium.
[0115] Table 4 Formula of cell culture medium without xenogeneic urine source
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[0118] Digest primary urine-derived cells and count them. Plate 1500 cells per well (96-well plate). Day0 uses primary urine cell culture medium (REGM medium) for culture, and Day1, Day3, and Day5 are replaced with corresponding groups The medium was cultured, 150 μl of medium per well; MTT detection was carried out on Day1, Day4, and Day7.
[0119] After the MTT test, the MTT results of groups 1-7 are as follows image 3 shown. From image 3 It can be seen that on Day7,...
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