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Rapid propagation method for dysosma versipellis

A fast technology for star anise lotus, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems that there are no reports of somatic embryos of star anise lotus plants, and achieve improved disease resistance, high plant regeneration frequency, and traits stable effect

Active Publication Date: 2015-11-04
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, except for the research results of our laboratory, there is no report about the somatic embryogenesis of the star anise plant

Method used

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  • Rapid propagation method for dysosma versipellis
  • Rapid propagation method for dysosma versipellis

Examples

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Effect test

Embodiment 1

[0039] A kind of rapid propagation method of star anise lotus, comprises the following steps:

[0040] Step 1: making sterile explants of star anise lotus, selecting leaves, roots or stems of star anise lotus as explants, placing the explants in a beaker, soaking them with amphoteric surfactants, and placing them in ultrasonic waves Vibrate for 10min, rinse with deionized water for 40min, gently scrub the dirt on the surface of the explant with a soft brush in the rinse process, then blot the moisture outside the explant with sterile filter paper and cut into 5mm 2 To obtain the aseptic explants, the explants of Star Anise are first soaked in an amphoteric surfactant for disinfection. The alkaline amphoteric surfactant of beetroot has good antibacterial properties and can inhibit the growth of some bacteria and fungi. Growth and reproduction, at the same time, the alkyl dimethyl betaine in the amphoteric surfactant has a good chelating ability for divalent copper, and the diva...

Embodiment 2

[0057] A kind of rapid propagation method of star anise lotus, comprises the following steps:

[0058] Step 1: making sterile explants of star anise lotus, selecting leaves, roots or stems of star anise lotus as explants, placing the explants in a beaker, soaking them with amphoteric surfactants, and placing them in ultrasonic waves Vibrate for 20min, rinse with deionized water for 50min, gently scrub the dirt on the surface of the explant with a soft brush during the rinse, then blot the moisture outside the explant with sterile filter paper and cut into 5mm 2 To obtain the aseptic explants, the explants of Star Anise are first soaked in an amphoteric surfactant for disinfection. The alkaline amphoteric surfactant of beetroot has good antibacterial properties and can inhibit the growth of some bacteria and fungi. Growth and reproduction, at the same time, the alkyl dimethyl betaine in the amphoteric surfactant has a good chelating ability for divalent copper, and the divalent...

Embodiment 3

[0075] A kind of rapid propagation method of star anise lotus, comprises the following steps:

[0076] Step 1: making sterile explants of star anise lotus, selecting leaves, roots or stems of star anise lotus as explants, placing the explants in a beaker, soaking them with amphoteric surfactants, and placing them in ultrasonic waves Vibrate for 15min, rinse with deionized water for 45min, gently scrub the dirt on the surface of the explant with a soft brush during the rinse, then blot the moisture outside the explant with sterile filter paper and cut into 5mm 2 To obtain the aseptic explants, the explants of Star Anise are first soaked in an amphoteric surfactant for disinfection. The alkaline amphoteric surfactant of beetroot has good antibacterial properties and can inhibit the growth of some bacteria and fungi. Growth and reproduction, at the same time, the alkyl dimethyl betaine in the amphoteric surfactant has a good chelating ability for divalent copper, and the divalent...

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Abstract

The invention discloses a rapid propagation method for dysosma versipellis. The rapid propagation method includes the following steps that 1, sterile dysosma versipellis explants are manufactured; 2, the sterile explants are cultured through an induction medium to obtain embryonic callus; 3, the embryonic callus is cultured through a first multiplication medium, and the embryonic callus is subcultured for 2-3 generations to obtain subcultured embryonic callus; 4, the subcultured embryonic callus is cultured through a differentiation medium to obtain somatic embryos; 5, the somatic embryos are cultured through a second multiplication medium to obtain subcultured somatic embryos; 6, the subcultured somatic embryos are cultured through a plant regeneration medium to obtain dysosma versipellis plants. According to the rapid propagation method, the obtained dysosma versipellis embryogenic cell can induce regeneration of the dysosma versipellis plants, and the dysosma versipellis plants have the advantages of being high in growth rate and plant regeneration frequency, stable in character and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for rapid propagation of star anise lotus. Background technique [0002] Podophyllotoxin (PTOX) is a cyclolignan lactone in lignans. It is a natural product with anti-tumor activity extracted from podophyllum plants. It is a synthetic etoposide ( The precursor of drugs such as etoposide (VP-16) and teniposide (teniposide, VM-26). Studies have shown that podophyllotoxin has special effects in the treatment of chronic diseases such as esophageal cancer and uterine cancer, and the market demand is huge. With the continuous development of new functions of podophyllotoxin and the increase of human cancer rate in recent years, its market demand will also increase rapidly. Podophyllotoxin is mainly extracted from perennial herbaceous plants of the Berberidaceae Berberidaceae, such as the genus Illicium genus, the genus Prunus genus, the genus Heliconia genus and the genus Podophy...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 谭小明周雅琴余丽莹韦莹周小雷
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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