Rapid propagation method for dysosma versipellis
A fast technology for star anise lotus, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems that there are no reports of somatic embryos of star anise lotus plants, and achieve improved disease resistance, high plant regeneration frequency, and traits stable effect
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[0038]
[0039] A kind of fast propagation method of star anise, comprising the following steps:
[0040] Step 1: make the aseptic explant of Illicium lobata, choose the leaf or root or stem of Illicium lobata as the explant, place the explant in a beaker, soak with amphoteric surfactant, and place in an ultrasonic wave Shake for 10min, rinse with deionized water for 40min, gently scrub the dirt on the surface of the explant with a soft brush during the rinsing process, then use sterile filter paper to absorb the moisture outside the explant and cut it into 5mm 2 to obtain the aseptic explants, the explants of the star anise are firstly soaked in amphoteric surfactants, and the amphoteric surfactants of beta-alkaline have good antibacterial properties and can inhibit some bacteria and fungi. Growth and reproduction, at the same time, the alkyl dimethyl betaine in the amphoteric surfactant has a good chelating ability to divalent copper, chelating the divalent copper from the...
Example Embodiment
[0056]
[0057] A kind of fast propagation method of star anise, comprising the following steps:
[0058] Step 1: make the aseptic explant of Illicium lobata, choose the leaf or root or stem of Illicium lobata as the explant, place the explant in a beaker, soak with amphoteric surfactant, and place in an ultrasonic wave Shake for 20min, rinse with deionized water for 50min, gently scrub the dirt on the surface of the explant with a soft brush during the rinsing process, then use sterile filter paper to absorb the moisture outside the explant and cut it into 5mm 2 to obtain the aseptic explants, the explants of the star anise are firstly soaked in amphoteric surfactants, and the amphoteric surfactants of beta-alkaline have good antibacterial properties and can inhibit some bacteria and fungi. Growth and reproduction, at the same time, the alkyl dimethyl betaine in the amphoteric surfactant has a good chelating ability to divalent copper, chelating the divalent copper from the...
Example Embodiment
[0074]
[0075] A kind of fast propagation method of star anise, comprising the following steps:
[0076] Step 1: make the aseptic explant of Illicium lobata, choose the leaf or root or stem of Illicium lobata as the explant, place the explant in a beaker, soak with amphoteric surfactant, and place in an ultrasonic wave Shake for 15min, rinse with deionized water for 45min, gently scrub the dirt on the surface of the explant with a soft brush during the rinsing process, and then use sterile filter paper to absorb the moisture outside the explant and cut it into 5mm 2 to obtain the aseptic explants, the explants of the star anise are firstly soaked in amphoteric surfactants, and the amphoteric surfactants of beta-alkaline have good antibacterial properties and can inhibit some bacteria and fungi. Growth and reproduction, at the same time, the alkyl dimethyl betaine in the amphoteric surfactant has a good chelating ability to divalent copper, chelating the divalent copper from...
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