Method for regenerating plant from camellia callus

A technology for callus and regenerated plants, applied in the biological field, can solve the problems of high callus induction frequency, failure to obtain regenerated plants, no patent application, etc., to achieve genetic transformation, high regeneration frequency, and simple operation technology. Effect

Inactive Publication Date: 2009-10-21
RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Long Zhenxiong (1981) used the pollen of Camellia oleifera as explants, and obtained a higher callus induction frequency on a suitable medium, and obtained green shoots and roots, but failed to obtain complete regeneration due to pollution plant
[0005] At present, there is no report of obtaining adventitious buds from the camellia callus, and no patent application

Method used

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Embodiment Construction

[0017] The present invention is further described in detail through the following examples, but the content of the present invention is not limited thereto.

[0018] The method for regenerating plant from this camellia callus of the present invention, the material is the mature fruit of Zhejiang red camellia in September. Rinse the collected fruit with tap water, soak it in washing powder for 10 minutes, and rinse with tap water. Then transfer to the aseptic ultra-clean table to remove the peel, then disinfect the surface with 75% ethanol by volume for 30 seconds, rinse 2 times with sterile water, and use 0.1% HgCl by weight 2 Soak in water for 15-20 minutes, rinse with sterile water 5-6 times. Peel off the inner and outer seed coats and inoculate on 1 / 2MS medium. The culture temperature is 25±2°C, the light intensity is 2500-3000Lx, and the light time is 12h / d. The following culture conditions are the same.

[0019] After 2 weeks of cultivation, the seedlings grow to a heig...

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PUM

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Abstract

The invention mainly relates to a method for regenerating a plant from camellia callus. The method has the following steps: stripping off the inner and outer seed coats of a camellia fruit seed, and inoculating the seed to a 1/2MS culture medium; when a sterile seedling grows above 3 cm, inoculating the soft leaf of the young plant to a callus induction culture medium which is MS + 0.5mg.L 6-BA+1.0mg.L 2,4-D; when the callus grows to get a diameter about 1 cm, shifting the callus to a callus differentiation culture medium which is MS+mg.L 6-BA20+0.1mg.L I BA+ mg.L KT0.1; when an indefinite bud grows to 0.5 cm, carrying out the separation and inoculating to a strong bud culture medium which is MS + 0.2mg.L 6-BA+0.05mg.L NAA; and when a bud stick grows to 4 to 5 cm, cutting off the basal of the bud stick, immersing the basal of the bud stick in 1,000 g.L I BA, and then inoculating to a MW + 0.2mg.L I BA+0.2mg.L NAA culture medium. The method has the advantages that: the method has a simple culture medium recipe, a simple and convenient operating process, short culture time, high regeneration frequency and a high propagation expansion coefficient, and facilitates the large-scale production of rare camellia plants and the realization of the genetic transformation of exogenous genes.

Description

technical field [0001] The invention relates to a camellia tissue culture method, belongs to the field of biotechnology, and is a method for regenerating plants from camellia callus. Background technique [0002] Camellia is a characteristic precious flower in my country and a world-renowned ornamental woody plant. Camellia is an important material for landscaping. It has beautiful flower colors, long flowering period, bright green leaves, colorful tree crowns, and the habit of growing well under tall tree crowns. It is widely used in park green spaces, natural scenic spots and scenic spots. Camellia is also a good potted plant. Certain dwarf shrub varieties are often used as potted plants. In addition to ornamental value, camellia also has high economic value. Its flowers and roots can be used as medicine, and its fruit can be used to extract oil. The yellow extract can be used as food coloring. [0003] In order to maintain the excellent traits of the parents of the cam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李纪元范正琪李辛雷田敏
Owner RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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