The invention relates to the technical field of
plant tissue culture, in particular to a production method of
adiantum, which comprises the following steps: A taking
adiantum sporophyl as the explant; B preparing an M1 culture medium for the
spore germination and the proliferation and subculture of prothallium; C germinating the
spore, transferring the explant onto the M1 culture medium after sterilizing the surface of the explant and putting the M1 culture medium in a culturing room for culture; D proliferating and subculturing the prothallium, dividing the prothallium group generated after the
spore is germinated into small blocks, and transferring the small blocks to a new M1 culture medium for proliferation and subculture; and E inducing the
sporophyte, taking the prothallium blocks, mixing the prothallium blocks with a blending solution,
grinding the mixture by a soymilk grinder, spraying the ground mixed liquid to a
sieve tray provided with matrix, and putting the
sieve tray in a
greenhouse for cultivating to obtain the
adiantum sporophyte plant. The invention utilizes incomplete
tissue culture to produce the adiantum, thereby reducing the generation of subculture, reducing the production cost, reducing the
mutation rate, increasing the production efficiency and saving the manpower.