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94results about How to "High multiplication factor" patented technology

Method for regenerating plant from camellia callus

The invention mainly relates to a method for regenerating a plant from camellia callus. The method has the following steps: stripping off the inner and outer seed coats of a camellia fruit seed, and inoculating the seed to a 1 / 2MS culture medium; when a sterile seedling grows above 3 cm, inoculating the soft leaf of the young plant to a callus induction culture medium which is MS + 0.5mg.L 6-BA+1.0mg.L 2,4-D; when the callus grows to get a diameter about 1 cm, shifting the callus to a callus differentiation culture medium which is MS+mg.L 6-BA20+0.1mg.L I BA+ mg.L KT0.1; when an indefinite bud grows to 0.5 cm, carrying out the separation and inoculating to a strong bud culture medium which is MS + 0.2mg.L 6-BA+0.05mg.L NAA; and when a bud stick grows to 4 to 5 cm, cutting off the basal of the bud stick, immersing the basal of the bud stick in 1,000 g.L I BA, and then inoculating to a MW + 0.2mg.L I BA+0.2mg.L NAA culture medium. The method has the advantages that: the method has a simple culture medium recipe, a simple and convenient operating process, short culture time, high regeneration frequency and a high propagation expansion coefficient, and facilitates the large-scale production of rare camellia plants and the realization of the genetic transformation of exogenous genes.
Owner:RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY

Method for culturing teak seedlings by spike culture and rapid propagation in container

The invention discloses a method for culturing teak seedlings by spike culture and rapid propagation in containers. High-quality single teak seeds are used as the parent plants; when growing to 4 to 5 centimeters high, the seedlings sown on a sand bed are transplanted into a parent plant containing cup filled with nutrient substrate, and placed on a lifting seedling culturing bed to be cultured; when the diameter of each parent plant is larger than 0.3 centimeter, the parent plant is cut off from the part 10 to 15 centimeters far from the basic part to promote side branches to germinate; when the seedlings are semi-lignified, side branches are cut off, used as cuttings, inserted into a cutting containing cup filled with substrate and placed on the lifting seedling culturing bed to be cultured; therefore, a large amount of high-quality teak seedlings maintaining the excellent hereditary characters of the parent plants can be propagated rapidly in a short time, and the success rate of cottage reaches 85.1 to 96.6 percent. The method for culturing teak seedlings by spike culture and rapid propagation in the containers is not limited by season or region, and can be used for obtaining excellent propagants in a poor land condition; the moisture and nutrition of the parent plants are easier to control; the method is beneficial to culture of desired cutting spikes; the coefficient of propagation is high; the method is simple; the operation is easy; the production cost is low; and the method for culturing teak seedlings can meet the needs in modern forestry development of China.
Owner:GUANGXI FORESTRY RES INST

Licorice rapid propagation seedling culture method

The invention relates to a licorice rapid propagation seedling tissue culture method. The method comprises steps of licorice aseptic seed germination culture, licorice aseptic seedling culture, licorice rapid propagation seedling induction and unrooted rapid propagation seedling rooting culture, the steps are respectively carried out in an MS solid medium containing 20-30g / L of sucrose under aseptic 24-26DEG C conditions, mediums for the rapid propagation seedling induction and the rapid propagation seedling rooting are added with a plant growth conditioning agent, and the pH of the MS solid medium before disinfection is adjusted to 5.9-6.0. The tissue culture method which is utilized in the invention to culture the licorice rapid propagation seedling has more practical meanings than cell culture and hairy root culture, and allows the growth rate to be rapid and the propagation multiple to be high to 6 times. The method of the invention, which allows the nursery stock growth to be stable and the culture to be uninfluenced by natural environments of natural disasters and the like, has the characteristics of simple process, high inductivity, good repeatability, high rapid propagation seedling increment multiple and the like.
Owner:TIANJIN UNIV

4H-SiC avalanche photodetector and its preparing method

This invention provides a 4H-SiC snowslide photo-electric detector having high internal gain under low breakdown voltage, not sensitive to visible light and infrared light and testing UV light, weak signals and single-photon signals and its preparation method, in which, the photo-electric detector includes a n+type 4H-SiC substrate, an n-type epitaxial absorption layer, an n-type epitaxial multiplying layer and a p+ epitaxial layer orderly grow from the bottom up on the substrate, a passive film is set on the surface of the device, the epitaxial layer has a p electrode with a weld set on it and an n electrode is set on the back of the substrate, in preparation, the epitaxial plate is cleaned normally to prepare all table-boards then oxidation layer as the passive layer of the chip, the p electrode region is etched to erode the oxidation layer at the electrode pattern place, sputtering Ti / Al / Au as the contact metal of the p electrode to form n-type ohm contact on the back of the substrate.
Owner:XIAMEN UNIV

Large-scale breeding method of Borneol Cinnamomum camphora

The invention relates to a large- scale breeding method of Borneol Cinnamomum camphora. The method comprises the following steps of: breeding a plantlet obtained through unique cuttage treatment by utilizing a current-growth lignified branch of strongly disease-resistant, no pest and disease damage and grown-up seed tree for about one year, acclimatizing, subculturing and propagating a sterile material after obtaining the sterile material with the current germinal sprout as an explant, secondly carrying out strong seeding rootage cultivation, and finally cultivating seeding in a greenhouse. According to the method disclosed by the invention, induced, propagated, strengthened and rooting culture mediums are optimized and improved, the stable growth of the sterile material can be effectively ensured, the sizes of sprouts are uniform, vitrification or defoliation of a tissue culture seedling can be avoided, the strong tissue culture seedlings can be obtained for sure, the repeatability is strong, an acclimation period and a subculture period are stable, a browning situation is reduced, a propagation coefficient is improved, the survival rate of planting in a greenhouse is improved, asubculture block mass cutting method determines the growth and the propagation coefficient of later-stage materials and can meet requirements of large-scale production.
Owner:SUNSHINE HORTICULTURE

Apple stock MM116 tissue culture rapid propagation method

The invention discloses an apple stock MM116 tissue culture rapid propagation method. The method comprises the steps as follows: firstly, conducting primary culture through adopting a culture medium containing MS+6-BA 0.5 mg / L+IBA 0.1 mg+PVP 500mg / L; secondly, conducting subculture by using MS+6-BA 0.6 mg / L+IBA 0.1 mg / L; thirdly, adopting a rooting culture medium of 1 / 2 MS+IBA 0.9 mg / L for rooting culture; fourthly, adopting pure saw dust for acclimatization and transplant. Through the method, the apple stock MM116 tissue culture rapid propagation is realized, a higher survival rate is guaranteed, the excellent characteristics of a stock is maintained, and a convenient method and approach are brought to the development and further expansion of the apple industry.
Owner:NORTHWEST A & F UNIV

Optical generation device and method for phase coding signal with tunable multiplication factor

The invention relates to an optical generation device and method for a phase coding signal with a tunable multiplication factor. The device comprises a laser, a DP-QPSK modulator, a microwave signal generator, an electric amplifier, a coding signal generator, a direct current power, a polarization controller, a polarizer, an optical amplifier, and a photo detector. The method comprises the step ofgenerating binary phase coding signals with different multiplication factors according to needs by using a non-linear modulation characteristic of the DP-QPSK modulator, wherein the multiplication factors can be any one of one to four, and the frequencies of the generated signals are tuned by adjusting the frequency of an input microwave signal. The device is based on an integrated modulator structure, has a simple system, has stable performance, overcomes defects that a traditional manner of generating a pulse compression signal in the frequency domain is limited by the rate and bandwidth ofan electronic device, has difficulty in generating a high-frequency signal or cannot generate the high-frequency signal and the generated signal cannot be tuned flexibly in frequency, greatly improves the frequency of generating the phase coding signal, and can produce phase coding signals with very large frequency tuning range.
Owner:EAST CHINA NORMAL UNIV

Production method of adiantum

The invention relates to the technical field of plant tissue culture, in particular to a production method of adiantum, which comprises the following steps: A taking adiantum sporophyl as the explant; B preparing an M1 culture medium for the spore germination and the proliferation and subculture of prothallium; C germinating the spore, transferring the explant onto the M1 culture medium after sterilizing the surface of the explant and putting the M1 culture medium in a culturing room for culture; D proliferating and subculturing the prothallium, dividing the prothallium group generated after the spore is germinated into small blocks, and transferring the small blocks to a new M1 culture medium for proliferation and subculture; and E inducing the sporophyte, taking the prothallium blocks, mixing the prothallium blocks with a blending solution, grinding the mixture by a soymilk grinder, spraying the ground mixed liquid to a sieve tray provided with matrix, and putting the sieve tray in a greenhouse for cultivating to obtain the adiantum sporophyte plant. The invention utilizes incomplete tissue culture to produce the adiantum, thereby reducing the generation of subculture, reducing the production cost, reducing the mutation rate, increasing the production efficiency and saving the manpower.
Owner:东莞市生物技术研究所

Method for culture and plant regeneration of ormosia microphylla in-vitro embryo

The invention belongs to the method for cultivating economic forests and particularly relates to a method for culture and plant regeneration of an ormosia microphylla in-vitro embryo. According to the method disclosed by the invention, the culture and plant regeneration of the ormosia microphylla in-vitro embryo is completed by adopting embryo induced culture, primary bud inducted culture, bud multiplication culture, strong seedling culture, and root induction culture. The method for culture and plant regeneration of the ormosia microphylla in-vitro embryo is higher in expanding propagation coefficient, short in germination time and high in propagation speed, and has an important theoretical significance and higher economic value as well as development utilization prospect for efficiently nursing rare and endangered ormosia microphylla resources, through the strong seedling culture, a grown-up seedling is high in survival rate after being transplanted, and the seedling is healthy and strong as well as tall and straight with good growth vigor.
Owner:FUJIAN AGRI & FORESTRY UNIV

Method for cultivating polygala fallax hemsl tissue culture seedlings based on multiple internodal stem segments integration

The invention provides a method for cultivating polygala fallax hemsl tissue culture seedlings based on multiple internodal stem segments integration. The method comprises the following steps: using of polygala fallax hemsl plants as explants, surface sterilization, primary culture, integrated cultivation, seedling hardening and transplantation. Thus, the polygala fallax hemsl tissue culture seedlings are finally obtained. According to the invention, multiple internodal stem segments are used as explants, the multiple internodal stem segments are put in a primary culture medium according to an independent formula from special studies to finish proliferation, rooting and other processes, and the polygala fallax hemsl tissue culture seedlings are cultivated by an integration method. By the method, propagation coefficient is raised, and high quality of nursery stock is maintained to meet introduction and popularization requirements. By the polygala fallax hemsl tissue culture technology, a lot of polygala fallax hemsl plants with excellent quality of female parents can be propagated within a short period of 30-50 d.
Owner:SANMING UNIV

Method for brewing alcohol-free primary pulp beer

ActiveCN105238607ARich flavorLow raw material cost and power consumptionMicroorganism based processesBeer brewingMarket potentialChemistry
The invention relates to a method for brewing alcohol-free primary pulp beer. Barley malt, burnt malt, special barley malt and special wheat malt are taken as raw materials, and the steps of smashing, saccharifying, filtering, adding hops, boiling, cooling, inoculating wheat beer yeast, temperature-controlled fermentation, storage, aftertreatment and the like are carried out, so that the alcohol-free primary pulp beer is obtained. The method for brewing the alcohol-free primary pulp beer has the advantages that burnt malt, common malt, special barley malt and special wheat malt are initiatively matched and used in raw materials for brewing the alcohol-free primary pulp beer; the alcohol-free beer brewed from malt juice by adopting the method provided by the invention is strong in flavour, content of residual fermentable sugar is extremely low, extra equipment is not needed for removing alcohol, and the raw material cost and the power consumption are low; the produced alcohol-free primary pulp beer meets the requirement of consumers on alcohol-free or low-alcohol drink and can be used for improving the current consumption status that kinds of alcohol-free beer are drab and taste is single, so that the alcohol-free primary pulp beer has great market potential.
Owner:SHANDONG AGRICULTURAL UNIVERSITY

Sugarcane rotation cultivation method of three harvests within two years

The invention discloses a sugarcane rotation cultivation method of three harvests within two years. The method comprises the steps of planting short-term crops such as soybean, corn, watermelon and the like in a sugarcane field in spring of the first year, transplanting sugarcane seedlings after the soybean, corn, watermelon and the like are harvested, harvesting sugarcane seeds and keeping perennial roots in February and March of the following year, and harvesting perennial root raw material sugarcane in December of the second year. By adopting the method, it is possible to harvest crops suchas the soybean, corn, watermelon and the like one time in two years in the sugarcane field and harvest the sugarcane twice, which not only utilizes lands reasonably, but also alleviates the obstacleof continuous sugarcane cropping, the combination of land use and land maintaining is achieved, the speed of sugarcane breeding is improved, and the upgrading of varieties is accelerated.
Owner:SUGARCANE RES INST GUANGXI ACADEMY OF AGRI SCI

Method for quickly propagating plukenetia volubilis linneo

The invention discloses a method for quickly propagating plukenetia volubilis linneo. The method includes steps of disinfecting the surfaces of explants which are current-year non-lignified branches of the plukenetia volubilis linneo; cultivating and inducing callus tissues of the explants; inducing differentiation and elongation of buds by the aid of the callus tissues; strengthening and rooting seedlings; exercising and transplanting the seedlings and the like to obtain regenerated plants. The method has the advantages that integral procedures can be carried out under controllable conditions without seasonal constraints, the propagation cycle can be shortened to a great extent, the propagation multiples can be increased, the method has important practical significance on meeting requirements on high-quality seedlings of the plukenetia volubilis linneo and accelerating popularization of improved varieties of the plukenetia volubilis linneo, and an efficient and feasible technical means can be provided for future research on genetic modification and the like on the plukenetia volubilis linneo.
Owner:HAINAN UNIVERSITY

Earthworm breeding method

The invention relates to an earthworm breeding method. The method comprises the following steps: A, preparing breeding materials; B, constructing an earthworm bed; C, throwing seedlings; D, supplementing materials; E, harvesting. By adopting the method, the survival rate of earthworms is increased remarkably. Compared with the prior art, the earthworm breeding method has the advantages that the propagation rate of the earthworms bred with the method is increased by 29.89 percent, and the death rate is lowered by 51.92 percent. Meanwhile, the quality of the earthworms is improved through the method. Compared with the prior art, the earthworm breeding method also has the advantages that the lengths of the earthworms bred with the method are increased by 20 percent, the average body weight is increased by 17.09 percent, and the protein content is increased by 15.91 percent.
Owner:CHONGQING CAIDIEGU ECOLOGICAL AGRI TECH CO LTD

Malus crabapples root grafting breeding method

The invention belongs to the seedling breeding field, particularly to a malus crabapples root grafting breeding method. The malus crabapples root grafting breeding method comprises the processes of stock selection, scion strip collection, grafting, grafting seedling planting and grafting seedling management. Main foots and side foots of Hubei crabapples seedlings are selected to serve as the stocks, the root grafting seedling growing is performed by a cleft grafting method, the specification management is performed in the late graft period, and the malus crabapples root grafting high-quality seedlings are cultivated. According to the malus crabapples root grafting breeding method, the grafting can be achieved in the indoor space and the operation is convenient, the efficiency is high, and the labor intensity is greatly reduced in comparison with the field grafting; the ordinary Hubei crabapples roots serve as the stocks and accordingly the resources are rich and the material selection is easy; the main foots and the side foots of the Hubei crabapples serve as the stocks, 6 to 7 root grafting stocks can be cut from a one-year Hubei crabapples seedling generally, and accordingly the propagation coefficient is improved.
Owner:HEBEI ACAD OF FORESTRY SCI

Tissue culture rapid propagation method of fast-growing Ulmus pumila

The invention relates to the technical field of plant tissue culture, in particular to a tissue culture rapid propagation method of fast-growing Ulmus pumila. The tissue culture rapid propagation method includes steps of aseptic explant obtaining, multiple-shoot inducement, tissue culture seeding rejuvenation, rooting culture and seedling hardening. The steps do not include primary culture, and obtained aseptic seedlings are directly subjected to rapid propagation, seedling hardening and rooting sequentially, so that tissue culture seedling rooting quality and seedling hardening survival rate are improved; retrieval in currently disclosed available documents about rapid propagation method shows that rapid propagation seedling emergence rate is highest, in other words, propagation coefficient of fast-growing Ulmus pumila can reach 4.5, rooting rate reaches 96.3%, and seedling hardening survival rate reaches 95%. A culture medium is improved by adding hormones different in concentration and type on the basis of a DKW culture medium to improve propagation efficiency, so that the rapid propagation problem of fast-growing Ulmus pumila is solved; compared with conventional steps, the tissue culture rapid propagation method is high in propagation coefficient and seedling emergence quality, and a good foundation is laid for fast-growing industrialized seedling cultivation or large-scale production and popularization.
Owner:SHANDONG FOREST GERMPLASM RESOURCES CENT

Quick propagation method of Tectona grandis

The invention discloses a fast propagation method of black ungold teak; the black ungold teak seedlings which are more than 30 centimeters in height can be obtained by the processes of collecting and treating branch, sterilization, induced culture, multiplication culture, radication culture, seedling hardening and seedbed management. The propagation materials can be easily obtained by the method and the propagation multiple is large; the method for producing seedlings in industrialization has the advantages that the operation is convenient, the cost for seedling production is low and a large number of seedlings can be cultured fast; therefore, the invention has a bright market application prospect.
Owner:XISHUANGBANNA TROPICAL BOTANICAL GARDEN CHINESE ACAD OF SCI

Tissue culture and rapid propagation method of hemionitis arifolia seedlings by virtue of green spherical body manner

The invention discloses a tissue culture and rapid propagation method of hemionitis arifolia seedlings by virtue of a green spherical body manner. Mediums used in the tissue culture and rapid propagation method comprise an induction medium, a propagation medium and a differential medium, wherein the induction medium is prepared from 0.4-1.0mg / L 1 / 6MS-1 / 4MS and TDZ, 0.1-0.3mg / L NAA and 400-800mg / L casein hydrolysate; the propagation medium is prepared from 0.1-0.3mg / L 1 / 6MS-1 / 4MS and TDZ, 0.5-1.0mg / L 6-BA, 0.1-0.3mg / L NAA and 400-800mg / L casein hydrolysate; the differential medium is prepared from 1 / 4MS-1 / 2MS and 3-6g / L active carbon; cane sugar and agar are added into all the mediums; the pH value of each medium is 5.80. The induction rate of the green spherical body is greater than 88%; the proliferation time is 11-12.5; the differentiation rate is greater than 98%; 1.5 million to 2 million of seedlings can be propagated from one green spherical body in one year; the survival rate s greater than 95%.
Owner:FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI

Independent and incoherent double-laser low-phase-noise 16-multiplying-frequency signal generation device

The invention relates to an independent and incoherent double-laser low-phase-noise 16-multiplying-frequency signal generation device, solving the problems that frequency doubling factors are low and high phase noise is introduced when an incoherent dual-light-source structure is adopted to generate millimeter wave signals. According to the device, the independent dual-light-source structure is adopted, an 8-multiplying-frequency signal is generated by utilizing two IQ (in-phase / quadrature) modulators, and a forward modulation technique involving a phase modulator, a photoelectric detector and a DC (direct current) break is combined to generate a 16-multiplying-frequency signal by utilizing three comb filters, so that the millimeter wave signals with high frequency doubling factors and low phase noise are easily obtained under the incoherent dual-light-source structure, and the device is especially suitable for fields such as optical fiber communication, optical fiber sensing, microwave photons and Radio over Fiber (RoF).
Owner:BEIJING JIAOTONG UNIV

Ultra-thin interstock grafting method

InactiveCN103782809AExpand the field of graftingHigh multiplication factorHorticultureSeedlingPlant cultivation
The invention relates to a grafting method, and belongs to the technical field of cultivation of plants. According to the ultra-thin interstock grafting method, the interstocks are needed for survival or the economical plants can be achieved through the interstocks. The ultra-thin interstock grafting method includes the following steps that firstly, rootstocks are selected; second, the interstocks are selected and processed; third, scions are selected; fourth, grating is performed. According to the technical scheme, the grafting field can be expanded, the expanding propogation coefficient is increased, the growth vigor of plants is adjusted, the stress resistance is enhanced, the seedling using cost is effectively reduced, the grating survival rate is high, the method is easy and convenient to perform, and high popularization and application value is achieved.
Owner:澄江县经济作物工作站

Capacitance multiplier with high multiplication constant

ActiveCN107565928AHigh multiplication factorLarge multiplication factorActive element networkCapacitanceCMOS
The invention discloses a capacitance multiplier with a high multiplication constant; the capacitance multiplier comprises a first cascode circuit module, a second cascode circuit module, a trsanscondutance amplifier circuit module, an on-chip capacitor, a first bias current source, a second bias current source and a third bias current source; the trsanscondutance amplifier circuit module is provided with a first input end and a second input end, wherein the first input end is connected with the first cascode circuit module output end, the second input end is connected with the second cascodecircuit module output end, and the trsanscondutance amplifier circuit module output end is connected with the on-chip capacitor; the high output impedance adjusting type cascode circuit structure is employed, and a current mirror structure is added on the trsanscondutance amplifier circuit module output end, thus greatly improving the capacitance multiplier multiplication constant, and forming a greater equivalent on-chip capacitor. The whole capacitance multiplier circuit structure only uses a CMOS transistor and the capacitor, is small in area, and high in multiplication constant.
Owner:NINGBO UNIV

Special culture medium for potato virus-free seedling transplanting and preparation method thereof

The invention discloses a special culture medium for potato virus-free seedling transplanting and a preparation method thereof. The culture medium is characterized by being prepared from the following components: (1) mother liquid, (2) konjac flour and carrageenan, (3) white granulated sugar and (4) tap water. The culture medium provided by the invention has strong universality, is simple and convenient to operate, reduces the amount of labor used and can effectively improve the production efficiency of miniature seed potatoes.
Owner:XINJIANG PRODION & CONSTR CORPS NO 6 DIV AGRI SCI INST +1

Manufacturing method for self-protection silicon-based APD array device with temperature compensation

The invention relates to a manufacturing method for a self-protection silicon-based APD array device with temperature compensation. The structure of the self-protection silicon-based APD array device comprises an avalanche photodiode (APD) array, a temperature-sensitive diode and a quenching resistor, wherein the avalanche photodiode (APD) array is composed of micro-unit APDs, the quenching resistor is connected with each micro-unit APD in series to form a unit, all the units are connected in parallel to form a linear array, and the linear array device is connected with the temperature-sensitive diode in a surface mounting manner. The APD array of the structure has the advantages of good temperature compensatory, high uniformity, fast response speed, low noise and the like.
Owner:哈尔滨伽马光电科技有限公司

Large-scale vegetative propagation method of Gaya seedlings

The present invention discloses a large-scale vegetative propagation method of Gaya seedlings. Through step (1) Gaya explant cultivation; (2) Gaya tissue culture aseptic material induction; (3) domestication and subsequent proliferation and propagation; (4) strong seedlings rooting; (5) greenhouse planting of seedlings, industrial cultivation of Gaya tissue culture seedlings can be realized, and finally, batched high-quality seedlings can be obtained in a short period of time.
Owner:SUNSHINE HORTICULTURE

Method for culturing in vitro embryos and regenerating plants of ormosia hosiei in western Hubei province

The invention provides a key technology for culturing in vitro embryos and regenerating plants of ormosia hosiei in western Hubei province, belonging to the technologies of preserving and massively propagating an ormosia hosiei seed resource. The problems that the ormosia hosiei seeds are difficult to obtain and easily eaten by rats, since the seeds are hard, the dried seed husks can not absorb water easily due to poor water permeability, the natural propagation capability and the spreading capability are not strong, the fructification age is late, natural regeneration is difficult, blooming and fructification require 3-5 years, and good seedlings are scarce in the prior art are solved. According to the key technology, full seeds fruiting in the current year are selected as a material; after 20-35 days of embryo induction cultivation, robustly growing embryo plants can sprout up; after 25-45 days of multiplication cultivation, 20-35 days of strong seedling cultivation and 25-35 days of rooting cultivation, seedlings can be formed and then transplanted. The survival rate can achieve over 95%, the propagation coefficient is high, the propagation time is short, the survival rate of seedling transplanting is high, and the seedlings which are robust, tall and straight have bright green leaves and are excellent and uniform in growth.
Owner:何碧珠

Method for producing regenerated plantlet of Valeriana amurensis P.Smirn calluses

The invention provides a method for producing a regenerated plantlet of Valeriana amurensis P.Smirn calluses and relates to a method for producing a regenerated plantlet of Valeriana amurensis P.Smirn. The method provided by the invention solves the problems that the number of the Valeriana amurensis P.Smirn is less, the breeding cycle is long and the proliferation efficiency is low. The method comprises the following steps of: (1), processing seeds of the Valeriana amurensis P.Smirn and then culturing the seeds to obtain seedlings; (2), culturing the seedlings to obtain sterile seedlings; (3) cutting off new tender leaves of the sterile seedlings and culturing the leaves to obtain calluses; then carrying out a successive transfer culture to obtain adventitious buds; (4), cutting off the adventitious buds with the calluses on the base part and culturing to obtain plantlets; and (5), cutting off the plantlets from the base part and immersing the plantlets in kinetin; and then culturingto form complete plants. The method provided by the invention has the advantages of simple formulation, simple operation process, short culture cycle, great proliferation coefficient (7.5-9.5 times are propagated every one month), high regeneration frequency and low cost; and the method is good for production in a large scale.
Owner:HARBIN INST OF TECH

Optical generation method and device of 32-frequency-multiplication millimeter wave signal

The invention relates to the technical field of radio frequency communication, in particular to an optical generation method and device for 32-frequency multiplication millimeter wave signals. Comprising the following steps: dividing a light wave into two orthogonal polarization directions, dividing an upper path of light into two paths of light for polarization modulation respectively, dividing a radio frequency signal into two paths for driving corresponding polarization modulators respectively, converging the output, dividing the light wave into two paths again, injecting the two paths of light into the corresponding polarization modulators respectively, converging the output again, and multiplexing a lower path of light. And after the optical carrier is suppressed, a 32-frequency-doubled millimeter wave signal is finally obtained through an optical amplifier. The method designed by the invention has the advantages of high frequency multiplication factor, no need of a filter, simple structure and the like, the quality of the generated signal is higher, the defect of low frequency multiplication factor of the existing millimeter wave generation method can be effectively made up, and the method is suitable for current and future wireless and optical fiber communication systems; the device can reduce the requirement on equipment, thereby realizing the generation of high frequency multiplication millimeter wave signals with lower cost and simple structure.
Owner:BEIJING INSTITUTE OF GRAPHIC COMMUNICATION +1

Rapid propagation method for novel apple varieties Ruiyang and Ruixue

The invention belongs to the technical field of fruit tree seedling cultivation, and discloses a rapid propagation method for novel apple varieties Ruiyang and Ruixue; a culture medium is adopted forMS+PVP 2.0 g.L primary culture; another culture medium is adopted for MS+6-BA 0.5 mg.L<-1> to 0.75 mg.L<-1>+IBA 0.02 mg.L<-1> to 0.05 mg.L<-1> subculture treatment; a rooting medium is adopted for 1 / 2MS+IBA 0.75 mg<-1> to 1.1 mg.L<-1> rooting culture; rapid propagation of apple varieties is achieved by hardening and transplanting seedlings with seedling substrate moss. The method disclosed by theinvention has important significance for producing high-quality late-maturing upgraded apple seedlings.
Owner:NORTHWEST A & F UNIV

Formula of tissue culture medium for production of American red maple saplings and culture method

InactiveCN107372124AHigh multiplication factorIncreased annual production capacityHorticulture methodsPlant tissue cultureSucroseSaccharum
The invention discloses a formula of a tissue culture medium for production of American red maple saplings and a culture method. The formula has the advantages that by improving on the basis of an MS culture medium, a red maple culture medium (HF) is invented; the American red maple is subjected to tissue culture by 25g / L of saccharose, 7g / L of agar and 0.010mg / L to 0.003mg / L of phenyl urea derivative (TDZ), so that the propagation speed of the American red maple is greatly accelerated; the propagation expanding coefficient of the American red maple is improved; after culturing for six weeks, the propagation expanding coefficient can increased by two times and reaches 6.5; the yearly output of the American red maple is increased, and the output and economic benefits are large.
Owner:山东大丰园农业有限公司
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