94results about How to "High multiplication factor" patented technology

The invention mainly relates to a method for regenerating a plant from camellia callus. The method has the following steps: stripping off the inner and outer seed coats of a camellia fruit seed, and inoculating the seed to a 1/2MS culture medium; when a sterile seedling grows above 3 cm, inoculating the soft leaf of the young plant to a callus induction culture medium which is MS + 0.5mg.L 6-BA+1.0mg.L 2,4-D; when the callus grows to get a diameter about 1 cm, shifting the callus to a callus differentiation culture medium which is MS+mg.L 6-BA20+0.1mg.L I BA+ mg.L KT0.1; when an indefinite bud grows to 0.5 cm, carrying out the separation and inoculating to a strong bud culture medium which is MS + 0.2mg.L 6-BA+0.05mg.L NAA; and when a bud stick grows to 4 to 5 cm, cutting off the basal of the bud stick, immersing the basal of the bud stick in 1,000 g.L I BA, and then inoculating to a MW + 0.2mg.L I BA+0.2mg.L NAA culture medium. The method has the advantages that: the method has a simple culture medium recipe, a simple and convenient operating process, short culture time, high regeneration frequency and a high propagation expansion coefficient, and facilitates the large-scale production of rare camellia plants and the realization of the genetic transformation of exogenous genes.

The invention discloses a method for culturing teak seedlings by spike culture and rapid propagation in containers. High-quality single teak seeds are used as the parent plants; when growing to 4 to 5 centimeters high, the seedlings sown on a sand bed are transplanted into a parent plant containing cup filled with nutrient substrate, and placed on a lifting seedling culturing bed to be cultured; when the diameter of each parent plant is larger than 0.3 centimeter, the parent plant is cut off from the part 10 to 15 centimeters far from the basic part to promote side branches to germinate; when the seedlings are semi-lignified, side branches are cut off, used as cuttings, inserted into a cutting containing cup filled with substrate and placed on the lifting seedling culturing bed to be cultured; therefore, a large amount of high-quality teak seedlings maintaining the excellent hereditary characters of the parent plants can be propagated rapidly in a short time, and the success rate of cottage reaches 85.1 to 96.6 percent. The method for culturing teak seedlings by spike culture and rapid propagation in the containers is not limited by season or region, and can be used for obtaining excellent propagants in a poor land condition; the moisture and nutrition of the parent plants are easier to control; the method is beneficial to culture of desired cutting spikes; the coefficient of propagation is high; the method is simple; the operation is easy; the production cost is low; and the method for culturing teak seedlings can meet the needs in modern forestry development of China.

The invention relates to a licorice rapid propagation seedling tissue culture method. The method comprises steps of licorice aseptic seed germination culture, licorice aseptic seedling culture, licorice rapid propagation seedling induction and unrooted rapid propagation seedling rooting culture, the steps are respectively carried out in an MS solid medium containing 20-30g/L of sucrose under aseptic 24-26DEG C conditions, mediums for the rapid propagation seedling induction and the rapid propagation seedling rooting are added with a plant growth conditioning agent, and the pH of the MS solid medium before disinfection is adjusted to 5.9-6.0. The tissue culture method which is utilized in the invention to culture the licorice rapid propagation seedling has more practical meanings than cell culture and hairy root culture, and allows the growth rate to be rapid and the propagation multiple to be high to 6 times. The method of the invention, which allows the nursery stock growth to be stable and the culture to be uninfluenced by natural environments of natural disasters and the like, has the characteristics of simple process, high inductivity, good repeatability, high rapid propagation seedling increment multiple and the like.

The invention discloses an apple stock MM116 tissue culture rapid propagation method. The method comprises the steps as follows: firstly, conducting primary culture through adopting a culture medium containing MS+6-BA 0.5 mg/L+IBA 0.1 mg+PVP 500mg/L; secondly, conducting subculture by using MS+6-BA 0.6 mg/L+IBA 0.1 mg/L; thirdly, adopting a rooting culture medium of 1/2 MS+IBA 0.9 mg/L for rooting culture; fourthly, adopting pure saw dust for acclimatization and transplant. Through the method, the apple stock MM116 tissue culture rapid propagation is realized, a higher survival rate is guaranteed, the excellent characteristics of a stock is maintained, and a convenient method and approach are brought to the development and further expansion of the apple industry.

The invention relates to the technical field of plant tissue culture, in particular to a production method of adiantum, which comprises the following steps: A taking adiantum sporophyl as the explant; B preparing an M1 culture medium for the spore germination and the proliferation and subculture of prothallium; C germinating the spore, transferring the explant onto the M1 culture medium after sterilizing the surface of the explant and putting the M1 culture medium in a culturing room for culture; D proliferating and subculturing the prothallium, dividing the prothallium group generated after the spore is germinated into small blocks, and transferring the small blocks to a new M1 culture medium for proliferation and subculture; and E inducing the sporophyte, taking the prothallium blocks, mixing the prothallium blocks with a blending solution, grinding the mixture by a soymilk grinder, spraying the ground mixed liquid to a sieve tray provided with matrix, and putting the sieve tray in a greenhouse for cultivating to obtain the adiantum sporophyte plant. The invention utilizes incomplete tissue culture to produce the adiantum, thereby reducing the generation of subculture, reducing the production cost, reducing the mutation rate, increasing the production efficiency and saving the manpower.

The invention belongs to the method for cultivating economic forests and particularly relates to a method for culture and plant regeneration of an ormosia microphylla in-vitro embryo. According to the method disclosed by the invention, the culture and plant regeneration of the ormosia microphylla in-vitro embryo is completed by adopting embryo induced culture, primary bud inducted culture, bud multiplication culture, strong seedling culture, and root induction culture. The method for culture and plant regeneration of the ormosia microphylla in-vitro embryo is higher in expanding propagation coefficient, short in germination time and high in propagation speed, and has an important theoretical significance and higher economic value as well as development utilization prospect for efficiently nursing rare and endangered ormosia microphylla resources, through the strong seedling culture, a grown-up seedling is high in survival rate after being transplanted, and the seedling is healthy and strong as well as tall and straight with good growth vigor.

The invention provides a method for cultivating polygala fallax hemsl tissue culture seedlings based on multiple internodal stem segments integration. The method comprises the following steps: using of polygala fallax hemsl plants as explants, surface sterilization, primary culture, integrated cultivation, seedling hardening and transplantation. Thus, the polygala fallax hemsl tissue culture seedlings are finally obtained. According to the invention, multiple internodal stem segments are used as explants, the multiple internodal stem segments are put in a primary culture medium according to an independent formula from special studies to finish proliferation, rooting and other processes, and the polygala fallax hemsl tissue culture seedlings are cultivated by an integration method. By the method, propagation coefficient is raised, and high quality of nursery stock is maintained to meet introduction and popularization requirements. By the polygala fallax hemsl tissue culture technology, a lot of polygala fallax hemsl plants with excellent quality of female parents can be propagated within a short period of 30-50 d.

The invention relates to a method for brewing alcohol-free primary pulp beer. Barley malt, burnt malt, special barley malt and special wheat malt are taken as raw materials, and the steps of smashing, saccharifying, filtering, adding hops, boiling, cooling, inoculating wheat beer yeast, temperature-controlled fermentation, storage, aftertreatment and the like are carried out, so that the alcohol-free primary pulp beer is obtained. The method for brewing the alcohol-free primary pulp beer has the advantages that burnt malt, common malt, special barley malt and special wheat malt are initiatively matched and used in raw materials for brewing the alcohol-free primary pulp beer; the alcohol-free beer brewed from malt juice by adopting the method provided by the invention is strong in flavour, content of residual fermentable sugar is extremely low, extra equipment is not needed for removing alcohol, and the raw material cost and the power consumption are low; the produced alcohol-free primary pulp beer meets the requirement of consumers on alcohol-free or low-alcohol drink and can be used for improving the current consumption status that kinds of alcohol-free beer are drab and taste is single, so that the alcohol-free primary pulp beer has great market potential.

The invention relates to an earthworm breeding method. The method comprises the following steps: A, preparing breeding materials; B, constructing an earthworm bed; C, throwing seedlings; D, supplementing materials; E, harvesting. By adopting the method, the survival rate of earthworms is increased remarkably. Compared with the prior art, the earthworm breeding method has the advantages that the propagation rate of the earthworms bred with the method is increased by 29.89 percent, and the death rate is lowered by 51.92 percent. Meanwhile, the quality of the earthworms is improved through the method. Compared with the prior art, the earthworm breeding method also has the advantages that the lengths of the earthworms bred with the method are increased by 20 percent, the average body weight is increased by 17.09 percent, and the protein content is increased by 15.91 percent.

The invention relates to a grafting method, and belongs to the technical field of cultivation of plants. According to the ultra-thin interstock grafting method, the interstocks are needed for survival or the economical plants can be achieved through the interstocks. The ultra-thin interstock grafting method includes the following steps that firstly, rootstocks are selected; second, the interstocks are selected and processed; third, scions are selected; fourth, grating is performed. According to the technical scheme, the grafting field can be expanded, the expanding propogation coefficient is increased, the growth vigor of plants is adjusted, the stress resistance is enhanced, the seedling using cost is effectively reduced, the grating survival rate is high, the method is easy and convenient to perform, and high popularization and application value is achieved.

The invention relates to a method for cultivating chrysanthemum potato, wherein it comprises inserting branches, shearing graft, and immerging, potato and managing seed bed. The invention can grow 100thousand chrysanthemum potato; after three year transplantation, each unit can grow 6000kg, the content of saponin can reach 3-4%.

The invention discloses a capacitance multiplier with a high multiplication constant; the capacitance multiplier comprises a first cascode circuit module, a second cascode circuit module, a trsanscondutance amplifier circuit module, an on-chip capacitor, a first bias current source, a second bias current source and a third bias current source; the trsanscondutance amplifier circuit module is provided with a first input end and a second input end, wherein the first input end is connected with the first cascode circuit module output end, the second input end is connected with the second cascodecircuit module output end, and the trsanscondutance amplifier circuit module output end is connected with the on-chip capacitor; the high output impedance adjusting type cascode circuit structure is employed, and a current mirror structure is added on the trsanscondutance amplifier circuit module output end, thus greatly improving the capacitance multiplier multiplication constant, and forming a greater equivalent on-chip capacitor. The whole capacitance multiplier circuit structure only uses a CMOS transistor and the capacitor, is small in area, and high in multiplication constant.

The invention discloses a special culture medium for potato virus-free seedling transplanting and a preparation method thereof. The culture medium is characterized by being prepared from the following components: (1) mother liquid, (2) konjac flour and carrageenan, (3) white granulated sugar and (4) tap water. The culture medium provided by the invention has strong universality, is simple and convenient to operate, reduces the amount of labor used and can effectively improve the production efficiency of miniature seed potatoes.

The invention relates to a manufacturing method for a self-protection silicon-based APD array device with temperature compensation. The structure of the self-protection silicon-based APD array device comprises an avalanche photodiode (APD) array, a temperature-sensitive diode and a quenching resistor, wherein the avalanche photodiode (APD) array is composed of micro-unit APDs, the quenching resistor is connected with each micro-unit APD in series to form a unit, all the units are connected in parallel to form a linear array, and the linear array device is connected with the temperature-sensitive diode in a surface mounting manner. The APD array of the structure has the advantages of good temperature compensatory, high uniformity, fast response speed, low noise and the like.

The present invention discloses a large-scale vegetative propagation method of Gaya seedlings. Through step (1) Gaya explant cultivation; (2) Gaya tissue culture aseptic material induction; (3) domestication and subsequent proliferation and propagation; (4) strong seedlings rooting; (5) greenhouse planting of seedlings, industrial cultivation of Gaya tissue culture seedlings can be realized, and finally, batched high-quality seedlings can be obtained in a short period of time.

The invention discloses a formula of a tissue culture medium for production of American red maple saplings and a culture method. The formula has the advantages that by improving on the basis of an MS culture medium, a red maple culture medium (HF) is invented; the American red maple is subjected to tissue culture by 25g/L of saccharose, 7g/L of agar and 0.010mg/L to 0.003mg/L of phenyl urea derivative (TDZ), so that the propagation speed of the American red maple is greatly accelerated; the propagation expanding coefficient of the American red maple is improved; after culturing for six weeks, the propagation expanding coefficient can increased by two times and reaches 6.5; the yearly output of the American red maple is increased, and the output and economic benefits are large.