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Special culture medium for potato virus-free seedling transplanting and preparation method thereof

A technology of culture medium and virus-free seedlings, applied in the field of culture medium, can solve the problems of easily injured seedlings, affecting the survival rate of bottle seedlings, and slow and weak growth of bottle seedlings

Inactive Publication Date: 2012-09-19
XINJIANG PRODION & CONSTR CORPS NO 6 DIV AGRI SCI INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The general-purpose medium is hard and difficult to clean, and it is easy to damage the seedlings if it is not washed thoroughly. If a certain amount of medium is transplanted to the ground, it is easy to be polluted and affect the survival rate of the bottle seedlings;
[0005] 2. The growth of bottle seedlings is relatively slow and thin
For the bottle seedlings to be put into the ground, it is required that the softer the better as long as they grow vigorously, but liquid culture requires more basic seedlings in production, the operation standard is high, and the seedling rate is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The special medium for potato virus-free seedlings to go to the ground is prepared from the following ingredients:

[0037] (1) Mother liquor;

[0038] The mother liquor is the following five mother liquors:

[0039] Mother liquor 1: potassium nitrate 18.5g / L, ammonium nitrate 16.2g / L, potassium dihydrogen phosphate 1.58g / L, magnesium sulfate 3.43 g / L, calcium chloride 4.38 / L, distilled water;

[0040] Mother liquor 2: manganese sulfate 3.32g / L, potassium iodide 0.156g / L, boric acid 1.235g / L, zinc sulfate 1.617 g / L, sodium molybdate 0.043g / L, copper sulfate 0.004g / L, cobalt chloride 0.0045g / L L, distilled water;

[0041] Mother liquor 3: disodium edetate 3.68g / L, ferrous sulfate 2.76g / L, distilled water;

[0042] Mother liquor 4: glycine 0.39g / L, thiamine hydrochloride 0.075g / L, pyridoxine 0.096g / L, niacin 0.095 g / L, distilled water;

[0043] Mother liquor 5: inositol 19.6g / L, distilled water;

[0044] The amount of mother solution 1-mother solution 5 added in each...

Embodiment 2

[0052] The special medium for potato virus-free seedlings to go to the ground is prepared from the following ingredients:

[0053] (1) Mother liquor;

[0054] The mother liquor is the following five mother liquors:

[0055] Mother liquor 1: potassium nitrate 19.2g / L, ammonium nitrate 16.8g / L, potassium dihydrogen phosphate 1.64 g / L, magnesium sulfate 3.51 g / L, calcium chloride 4.43g / L, distilled water;

[0056] Mother liquor 2: manganese sulfate 3.39 g / L, potassium iodide 0.166 g / L, boric acid 1.241 g / L, zinc sulfate 1.622 g / L, sodium molybdate 1.051 g / L, copper sulfate 0.005 g / L, cobalt chloride 0.0052 g / L L, distilled water;

[0057] Mother liquor 3: disodium edetate 3.75g / L, ferrous sulfate 2.80g / L, distilled water;

[0058] Mother liquor 4: glycine 0.45g / L, thiamine hydrochloride 0.083g / L, pyridoxine 0.12 g / L, niacin 0.13 g / L, distilled water;

[0059] Mother liquor 5: inositol 20.3g / L, distilled water;

[0060] The amount of mother solution 1-mother solution 5 added in...

Embodiment 3

[0068] The special medium for potato virus-free seedlings to go to the ground is prepared from the following ingredients:

[0069] (1) Mother liquor;

[0070] The mother liquor is the following five mother liquors:

[0071] Mother liquor 1: potassium nitrate 19g / L, ammonium nitrate 16.6g / L, potassium dihydrogen phosphate 1.61g / L, magnesium sulfate 3.48g / L, calcium chloride 4.4g / L, distilled water;

[0072] Mother liquor 2: manganese sulfate 3.36 g / L, potassium iodide 0.16 g / L, boric acid 1.238 g / L, zinc sulfate 1.62 g / L, sodium molybdate 0.54 g / L, copper sulfate 0.004 g / L, cobalt chloride 0.0052 g / L L, distilled water;

[0073] Mother liquor 3: disodium edetate 3.7g / L, ferrous sulfate 2.80 g / L, distilled water;

[0074] Mother liquor 4: glycine 0.42g / L, thiamine hydrochloride 0.08g / L, pyridoxine 0.1 g / L, niacin 0.1 g / L, distilled water;

[0075] Mother liquor 5: Inositol 20g / L, distilled water;

[0076] The amount of mother solution 1-mother solution 5 added in each liter...

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Abstract

The invention discloses a special culture medium for potato virus-free seedling transplanting and a preparation method thereof. The culture medium is characterized by being prepared from the following components: (1) mother liquid, (2) konjac flour and carrageenan, (3) white granulated sugar and (4) tap water. The culture medium provided by the invention has strong universality, is simple and convenient to operate, reduces the amount of labor used and can effectively improve the production efficiency of miniature seed potatoes.

Description

technical field [0001] The invention relates to a culture medium, in particular to a special culture medium for virus-free potato seedlings and a preparation method thereof. Background technique [0002] Virus disease is one of the main diseases that affect the yield and quality of crops, especially for crops that are propagated by asexual reproduction, such as potatoes, sweet potatoes, strawberries, fruit trees and flowers, etc., after multiple generations of asexual reproduction, a large number of viruses in plants will accumulate, resulting in Seed degradation, growth inhibition, yield decline and quality deterioration, and the use of shoot tip culture supplemented by chemical or high temperature treatment can better play the role of plant virus-free and obtain virus-free seedlings. At present, my country has established a rapid propagation system for different organs and tissues of various plants, which can not only detoxify and rapidly propagate, but also preserve endan...

Claims

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Application Information

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IPC IPC(8): C05G3/00
Inventor 李东方张爱萍赵亮陈英刘江娜王航杨林栋
Owner XINJIANG PRODION & CONSTR CORPS NO 6 DIV AGRI SCI INST
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