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Tissue culture rapid propagation method of fast-growing Ulmus pumila

A technique for tissue culture, rapid propagation and white elm, applied in the field of plant tissue culture, can solve problems such as inability to guide production well, unsystematic method of white elm tissue culture, etc. Complex problems and the effect of improving reproductive efficiency

Inactive Publication Date: 2016-04-20
SHANDONG FOREST GERMPLASM RESOURCES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem that the tissue culture method of Ulmus undatus in the prior art is not systematic and cannot guide production well, the present invention provides a complete fast-growing method for tissue culture and rapid propagation of Ulmus undatus

Method used

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  • Tissue culture rapid propagation method of fast-growing Ulmus pumila
  • Tissue culture rapid propagation method of fast-growing Ulmus pumila
  • Tissue culture rapid propagation method of fast-growing Ulmus pumila

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Embodiment

[0025] From May to June, new shoots and shoots were taken as explants, soaked in saturated clean water for 5-10 minutes, then lightly brushed the surface with a brush, and then rinsed with running water for 30-60 minutes; Disinfect with ~75% alcohol for 30 seconds, rinse with sterile water for 2 to 3 times, then disinfect with 1% sodium hypochlorite solution for 8 minutes, and then rinse with sterile water for 5 to 8 times, each time not less than 3 minutes, and finally put in a small amount of sterile water Water, to prevent the explants from being inactivated due to lack of water caused by too long inoculation time. When inoculating, use sterile filter paper to absorb excess water on the surface of the explants, and inoculate them on rapid propagation media containing different hormone ratios (see Table 1). Cultivate at 25 (±2)°C, light for 14~16h, and light intensity 3000lx. After 1 week, the buds began to differentiate, and the clustered buds grew steadily in 30-40 days. ...

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Abstract

The invention relates to the technical field of plant tissue culture, in particular to a tissue culture rapid propagation method of fast-growing Ulmus pumila. The tissue culture rapid propagation method includes steps of aseptic explant obtaining, multiple-shoot inducement, tissue culture seeding rejuvenation, rooting culture and seedling hardening. The steps do not include primary culture, and obtained aseptic seedlings are directly subjected to rapid propagation, seedling hardening and rooting sequentially, so that tissue culture seedling rooting quality and seedling hardening survival rate are improved; retrieval in currently disclosed available documents about rapid propagation method shows that rapid propagation seedling emergence rate is highest, in other words, propagation coefficient of fast-growing Ulmus pumila can reach 4.5, rooting rate reaches 96.3%, and seedling hardening survival rate reaches 95%. A culture medium is improved by adding hormones different in concentration and type on the basis of a DKW culture medium to improve propagation efficiency, so that the rapid propagation problem of fast-growing Ulmus pumila is solved; compared with conventional steps, the tissue culture rapid propagation method is high in propagation coefficient and seedling emergence quality, and a good foundation is laid for fast-growing industrialized seedling cultivation or large-scale production and popularization.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of fast-growing elm elm. Background technique [0002] White elm ( Ulmus pumila ) is a plant of the genus Ulmaceae, with well-developed root system, strong adaptability, cold and drought tolerance, and moderate saline-alkali tolerance. It can be used as afforestation and greening tree species in coastal saline-alkali land. The survival rate of the method is low and the propagation period is long, which affects its propagation and promotion. [0003] At present, domestic research on Ulmus chinensis mainly focuses on resource conservation, hybrid breeding, seedling selection, and unconventional breeding. Cell and tissue culture are rarely used in the genetic improvement of Ulmus ulmis. For explants, the induction effect of stem segments is better, but the differentiation effect of leaves is not good. Acetic acid ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 韩义张占彪李文清解孝满韩彪鲁仪增杨海平刘鵾王倩
Owner SHANDONG FOREST GERMPLASM RESOURCES CENT
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