Method for quickly propagating plukenetia volubilis linneo

A technology of South American star oil rattan and star oil rattan, which is applied in the field of rapid propagation of South American star oil rattan, can solve the problem of low induction rate of adventitious buds, and achieve the effects of shortening the propagation cycle, good growth and increasing reproduction

Active Publication Date: 2017-01-25
HAINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method has successfully obtained the in vitro regenerated plants of the South American star oil vine, the induction rate of its adventitious buds is not high (92.7%), and the research on the induction and differentiation of callus has not been carried out on the explants.

Method used

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  • Method for quickly propagating plukenetia volubilis linneo
  • Method for quickly propagating plukenetia volubilis linneo
  • Method for quickly propagating plukenetia volubilis linneo

Examples

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Embodiment 1

[0084] Embodiment 1, a kind of method of South American star oil rattan seedling cultivation

[0085] 1. Explant collection and surface disinfection

[0086] Cut the unlignified South American star oil rattan branches that grow vigorously in the current year and bring them back in sealed bags, and carry out surface disinfection on the collected fresh branches. Specific steps are as follows:

[0087] 1. Cut the branches into stems about 1.5cm long, put them in a 200ml beaker, and soak them with an appropriate amount of detergent (Diaopai detergent, purchased from Carrefour supermarket; catalog number GB9985-2000) for 30 minutes. Shake the beaker gently every 3-5 minutes, rinse it under running water for 30 minutes after soaking, and then place it on the ultra-clean workbench to obtain the soaked explants;

[0088] 2. Disinfect the soaked explants with 70% ethanol for 30 seconds, wash them with sterile water for 5 times; then disinfect them with 2% NaCl for 8 minutes, wash the...

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Abstract

The invention discloses a method for quickly propagating plukenetia volubilis linneo. The method includes steps of disinfecting the surfaces of explants which are current-year non-lignified branches of the plukenetia volubilis linneo; cultivating and inducing callus tissues of the explants; inducing differentiation and elongation of buds by the aid of the callus tissues; strengthening and rooting seedlings; exercising and transplanting the seedlings and the like to obtain regenerated plants. The method has the advantages that integral procedures can be carried out under controllable conditions without seasonal constraints, the propagation cycle can be shortened to a great extent, the propagation multiples can be increased, the method has important practical significance on meeting requirements on high-quality seedlings of the plukenetia volubilis linneo and accelerating popularization of improved varieties of the plukenetia volubilis linneo, and an efficient and feasible technical means can be provided for future research on genetic modification and the like on the plukenetia volubilis linneo.

Description

technical field [0001] The invention belongs to the technical field of cell engineering tissue culture, and in particular relates to a method for rapid propagation of vine vine. Background technique [0002] Plukenetia volubilis L., also known as Plukenetia volubilis L., is also known as Plukenetia volubilis L., Sacha Inchi in Inca language, etc. It is a perennial evergreen woody vine oil crop of Euphorbiaceae, native to the tropical regions of the Andes in South America. rainforest area. The plant can bloom and bear fruit in the same year of planting, and enters the high-yield period in 2-3 years, and the high-yield period lasts for more than 10 years. The oil extracted from the seeds of the South American star oil vine contains high unsaturated fatty acids (especially linolenic acid and linoleic acid), V A and V E It has obvious effects on adjusting blood lipids, preventing cardiovascular diseases, maintaining skin and anti-aging, etc. It is widely used in food, pharmac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 宋希强于旭东王榜琴马香李霖明余文刚钟云芳杨泽秀张哲
Owner HAINAN UNIVERSITY
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