The invention provides a key technology for culturing
in vitro embryos and regenerating plants of
ormosia hosiei in western Hubei province, belonging to the technologies of preserving and massively propagating an
ormosia hosiei seed resource. The problems that the
ormosia hosiei seeds are difficult to obtain and easily eaten by rats, since the seeds are hard, the dried seed husks can not absorb water easily due to poor water permeability, the natural propagation capability and the spreading capability are not strong, the
fructification age is late,
natural regeneration is difficult, blooming and
fructification require 3-5 years, and good seedlings are scarce in the prior art are solved. According to the key technology, full seeds fruiting in the current year are selected as a material; after 20-35 days of
embryo induction cultivation, robustly growing
embryo plants can sprout up; after 25-45 days of multiplication cultivation, 20-35 days of strong
seedling cultivation and 25-35 days of rooting cultivation, seedlings can be formed and then transplanted. The
survival rate can achieve over 95%, the propagation coefficient is high, the
propagation time is short, the
survival rate of
seedling transplanting is high, and the seedlings which are robust, tall and straight have bright green leaves and are excellent and uniform in growth.