Method for artificially promoting natural regeneration of silver fir

Abstract

The application discloses a method for artificially promoting natural regeneration of Tsuga chinensis, and comprises the following steps: forest thinning: removing broad-leaved trees in a Tsuga chinensis community, forming a forest gap around Tsuga chinensis mother trees and undergrowth seedlings, and retaining a canopy density of 0.5-0.6 after felling; artificial land preparation: cleaning and piling up litter on the ground in the forest gap to the periphery of the Tsuga chinensis seedling, and loosening the soil in the forest gap to a depth of 2cm-3cm; tending management: cleaning up weeds and grasses and harmful organisms around the Tsuga chinensis seedling in the forest gap, and performing seedling cultivation and topdressing. The method forms a forest gap through forest thinning, increases the forest understory light, promotes the growth of Tsuga chinensis seedlings, increases the contact probability of Tsuga chinensis seeds and soil through artificial soil loosening, improves the germination rate of Tsuga chinensis seeds, and promotes the growth of Tsuga chinensis seedlings through tending management, so that the purpose of promoting the regeneration of Tsuga chinensis forest is achieved.

Description

Technical Field

[0001] This invention belongs to the field of Cathaya argyrophylla planting technology, specifically relating to a method for artificially promoting the natural regeneration of Cathaya argyrophylla. Background Technology

[0002] Cathaya argyrophylla is a monotypic species of the genus Cathaya in the family Pinaceae. It is a relict plant endemic to my country and has been listed as a Class I protected wild plant in China. Historical climate change has led to a significant reduction in the distribution area and a drastic decline in population size, resulting in low genetic diversity and hindered gene flow. This has caused significant reproductive difficulties, leading to poor population regeneration and ecological adaptability. Even minor human-induced damage and logging pose a serious threat to the survival of the population. These factors, in turn, cause further population decline and shrinkage, creating a vicious cycle. Cathaya argyrophylla is classified as EN (Critically Endangered) by the IUCN, belonging to a very small population of wild plants.

[0003] Currently, in their natural state, Cathaya argyrophylla mostly grows on narrow mountain ridges or the tops of cap-shaped rocky hills, or even in the crevices of cliffs and precipices, where the soil is infertile and natural regeneration is extremely poor. Therefore, artificial propagation has become an important method for the protection and population restoration of Cathaya argyrophylla. While seed propagation is simpler and easier than other methods, it primarily relies on artificial seedling cultivation. After the seeds germinate and emerge, the seedlings are transplanted into the natural environment, which involves a large workload and high technical difficulty, hindering large-scale regeneration of Cathaya argyrophylla forests. Summary of the Invention

[0004] The purpose of this invention is to provide a method for artificially promoting the natural regeneration of Cathaya argyrophylla, which can promote the regeneration of Cathaya argyrophylla forests and is beneficial to expanding and protecting the Cathaya argyrophylla population.

[0005] To achieve the above objectives, a specific embodiment of the present invention provides the following technical solution:

[0006] A method for artificially promoting the natural regeneration of Cathaya argyrophylla includes:

[0007] Forest thinning: Appropriately remove broad-leaved trees from the Cathaya argyrophylla community to create forest gaps around the mother trees and understory saplings and seedlings. After thinning, the canopy closure of the forest stand should be maintained at 0.5-0.6.

[0008] Manual land preparation: Clear and pile the fallen leaves in the open space inside the forest gap around the saplings of Cathaya argyrophylla, and loosen the soil in the open space to a depth of about 2-3 cm.

[0009] Nurturing and management: Clear away weeds, shrubs, and harmful organisms around the young Cathaya argyrophylla seedlings in the forest gaps that affect their growth, and cultivate the base of the trees and apply fertilizer.

[0010] In one or more embodiments of the present invention, bamboo fiber is mixed into the soil during tilling and loosening, wherein the bamboo fiber is first loaded with ammonium sulfate and then wrapped with a carboxymethyl cellulose membrane containing humic acid.

[0011] In one or more embodiments of the present invention, the mass ratio of humic acid to carboxymethyl cellulose in the carboxymethyl cellulose membrane is 1:(1-2).

[0012] In one or more embodiments of the present invention, the mulching and fertilization operation involves deep tilling of the soil around the outside of the Cathaya argyrophylla tree to a depth of 10-20 cm, during which perlite and vermiculite are mixed into the soil.

[0013] The perlite is prepared by first loading a growth-promoting substance and then coating it with an alkylated starch film; the vermiculite is prepared by first loading a growth-promoting substance and then coating it with a dextrin film.

[0014] In one or more embodiments of the present invention, the mass ratio of the perlite to the soil is (3-5):20, and the mass ratio of the vermiculite to the perlite is 1:(1-3).

[0015] In one or more embodiments of the present invention, the perlite has a mesh size of 60-80 mesh; and / or, the vermiculite has a mesh size of 60-80 mesh.

[0016] In one or more embodiments of the present invention, the perlite is prepared by: making a growth-promoting substance into a nutrient solution, mixing the nutrient solution and perlite, soaking and filtering to obtain perlite loaded with the growth-promoting substance.

[0017] Perlite loaded with growth-promoting substances and alkylated starch slurry are mixed, filtered, and dried to obtain perlite coated with an alkylated starch film.

[0018] In one or more embodiments of the present invention, the alkylated starch slurry is mixed with humic acid.

[0019] In one or more embodiments of the present invention, the vermiculite is prepared by: making a growth-promoting substance into a nutrient solution, mixing the nutrient solution and vermiculite, soaking and filtering to obtain vermiculite loaded with the growth-promoting substance;

[0020] Vermiculite loaded with growth-promoting substances and dextrin solution were mixed, filtered, and dried to obtain vermiculite coated with an alkylated starch film.

[0021] In one or more embodiments of the present invention, the dextrin membrane on the vermiculite is further coated with an alkylated starch membrane.

[0022] Compared with existing technologies, this invention improves understory light by thinning the forest stand, thereby promoting the growth of Cathaya argyrophylla seedlings; it increases the contact between Cathaya argyrophylla seeds and soil by artificial tilling and loosening the soil, thereby promoting seed germination and growth; and it promotes the growth of Cathaya argyrophylla seedlings through tending and management, thus achieving the goal of promoting the regeneration of Cathaya argyrophylla forests. Detailed Implementation

[0023] To enable those skilled in the art to better understand the technical solutions in this disclosure, the technical solutions in the embodiments of this disclosure will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of this disclosure, and not all embodiments. Based on the embodiments in this disclosure, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of this disclosure.

[0024] A specific embodiment of the present invention provides a method for artificially promoting the natural regeneration of Cathaya argyrophylla, comprising:

[0025] Forest thinning: Appropriately remove broad-leaved trees from the Cathaya argyrophylla community to create forest gaps around the mother trees and understory saplings and seedlings. After thinning, the canopy closure of the forest stand should be maintained at 0.5-0.6.

[0026] Manual land preparation: Clear and pile the fallen leaves in the open space inside the forest gap around the saplings of Cathaya argyrophylla, and loosen the soil in the open space to a depth of about 2-3 cm.

[0027] Nurturing and management: Clear away weeds, shrubs, and harmful organisms around the young Cathaya argyrophylla seedlings in the forest gaps that affect their growth, and cultivate the base of the trees and apply fertilizer.

[0028] Specifically, firstly, thinning creates forest gaps, adjusting the canopy density and increasing light penetration under the trees, thus promoting the growth of Cathaya argyrophylla seedlings. Secondly, loosening the soil improves soil aeration, promotes root growth, and facilitates the reproduction of soil microorganisms, enhancing the decomposition of soil organic matter, which in turn further promotes root growth.

[0029] Secondly, after the seeds of Cathaya argyrophylla mature, they will naturally fall to the ground. By tilling and loosening the soil, the seeds can be buried in the soil, increasing their contact with the soil and promoting germination and growth, thus promoting the regeneration of Cathaya argyrophylla forests. In addition, by planting mulch and applying fertilizer, sufficient nutrients are provided for the growth of Cathaya argyrophylla seedlings, promoting their rapid growth.

[0030] Furthermore, during tilling and loosening of the soil, bamboo fiber is mixed into the soil. Ammonium sulfate is first loaded onto the bamboo fiber, and then a carboxymethyl cellulose membrane containing humic acid is wrapped around it.

[0031] Specifically, the bamboo fiber mesh size is 50-60 mesh, and the mixing mass ratio of bamboo fiber to soil is (3-5):20. Carboxymethyl cellulose membranes are degradable in soil. Upon degradation, they first release fulvic acid, followed by the release of ammonium sulfate. The initially released fulvic acid not only enhances enzyme activity and promotes nutrient absorption by roots, but also, through the complexation of its active groups with the released ammonium sulfate, prevents nitrogen loss, thus better facilitating nitrogen absorption by the roots.

[0032] In addition, bamboo fiber has strong water absorption. After the carboxymethyl cellulose membrane wrapped around it degrades, the bamboo fiber can absorb water more effectively in the soil. Ammonium sulfate is easily soluble in water. Therefore, as the bamboo fiber continues to absorb water and degrades in the soil, it can accelerate the release of ammonium sulfate and promote the absorption of nitrogen by the silver fir.

[0033] Furthermore, in the carboxymethyl cellulose membrane, the mass ratio of humic acid to carboxymethyl cellulose is 1:(1-2).

[0034] Specifically, by controlling the amount of fulvic acid used, fulvic acid can better promote the growth of Cathaya argyrophylla.

[0035] Furthermore, the fertilization and top dressing operation involves deep tilling of the soil around the outside of the Cathaya argyrophylla tree to a depth of 10-20 cm, during which perlite and vermiculite are mixed into the soil. The perlite is prepared by first loading growth-promoting substances and then coating them with an alkylated starch film; the vermiculite is prepared by first loading growth-promoting substances and then coating them with a dextrin film.

[0036] Specifically, when applying topdressing, perlite and vermiculite are mixed into the soil simultaneously. On one hand, perlite and vermiculite increase soil looseness and aeration, promoting the growth of Cathaya argyrophylla. On the other hand, after being coated, the perlite and vermiculite can slowly release growth-promoting substances through the slow degradation of the coating layer, continuously providing the nutrients needed for Cathaya argyrophylla growth. Moreover, in soil, dextrin degrades faster than starch. After alkylation treatment, starch becomes more hydrophobic, further slowing its degradation rate in the soil. This allows the growth-promoting substances loaded with vermiculite to be released first, followed by those loaded with perlite, ensuring a continuous supply of nutrients for Cathaya argyrophylla absorption over a longer period.

[0037] Furthermore, the mass ratio of perlite to soil is (3-5):20, and the mass ratio of vermiculite to perlite is 1:(1-3). The mesh size of perlite is 60-80 mesh, and the mesh size of vermiculite is 60-80 mesh. By controlling the mixing ratio of perlite and vermiculite in the soil, a continuous supply of nutrients for the silver fir can be provided. Simultaneously, by controlling the mesh size of perlite and vermiculite, the looseness and permeability of the soil can be better regulated.

[0038] Furthermore, growth-promoting substances include one or more of the following elements: phosphorus, potassium, magnesium, sulfur, nitrogen, and calcium. These elements can promote root development and seedling growth. Specifically, potassium phosphate, magnesium sulfate, ammonium chloride, and calcium chloride can be selected.

[0039] Furthermore, the preparation of perlite is as follows: the growth-promoting substance is made into a nutrient solution, the nutrient solution is mixed with perlite, and after soaking and filtration, perlite loaded with the growth-promoting substance is obtained; the perlite loaded with the growth-promoting substance is mixed with alkylated starch slurry, filtered and dried to obtain perlite coated with an alkylated starch film.

[0040] Specifically, a nutrient solution is prepared by dissolving potassium phosphate, magnesium sulfate, ammonium chloride, and calcium chloride in water. This solution is then used to soak perlite, allowing it to absorb and load these substances. Next, an alkylated starch slurry is used to coat the perlite, and after drying, a film is formed, effectively encapsulating these substances within the perlite. Alternatively, these substances can be added to the alkylated starch slurry to increase their loading within the perlite.

[0041] Furthermore, humic acid is mixed into the alkylated starch slurry.

[0042] Specifically, fulvic acid can promote the activity of various enzymes in the soil, such as promoting the decomposition of starch by amylase for root absorption. By adding fulvic acid to alkylated starch slurry, when the alkylated starch film covering perlite degrades, the exposed fulvic acid can promote the accelerated decomposition of alkylated starch by amylase. This promotes root absorption and further accelerates the degradation of the alkylated starch film on the outside of the perlite, thereby accelerating the release of growth-promoting substances from the perlite.

[0043] Furthermore, the preparation of vermiculite is as follows: the growth-promoting substance is made into a nutrient solution, the nutrient solution and vermiculite are mixed, soaked and filtered to obtain vermiculite loaded with the growth-promoting substance; the vermiculite loaded with the growth-promoting substance is mixed with a dextrin solution, filtered and dried to obtain vermiculite coated with an alkylated starch film.

[0044] Specifically, potassium phosphate, magnesium sulfate, ammonium chloride, and calcium chloride are dissolved in water to prepare a nutrient solution. The vermiculite is then soaked in this solution to adsorb and load these substances. Finally, a dextrin solution is used to encapsulate these substances within the vermiculite. To increase the loading of growth-promoting substances on the vermiculite, these substances can be added to the dextrin solution to load them onto a film layer outside the vermiculite.

[0045] Furthermore, the dextrin membrane on the vermiculite is also coated with an alkylated starch membrane.

[0046] Specifically, alkylated starch degrades at a slower rate than dextrin. The alkylated starch membrane slows down the release of growth-promoting substances from vermiculite, while the rapid degradation of the dextrin membrane after the alkylated starch membrane degrades can accelerate the release of growth-promoting substances.

[0047] The present invention will be further described in detail below with reference to specific embodiments.

[0048] In this invention, the fertilizer application rate during deep tillage and topdressing is 100g / plant. The fertilizer used is a commercially available common fertilizer. For example, Stanley fertilizer is selected. The fertilizer contains 15% total nitrogen (N), water-soluble phosphorus (P2O5), and soluble potassium (K2O), and the total nutrient content is ≥45%. The white dextrin used in this invention was purchased from Aladdin.

[0049] This invention selects a Cathaya argyrophylla forest located in a certain area of ​​Hunan Province for the experiment, in order to reduce the impact of differences in geographical environment and climate between different experimental sites. Experimental sites No. 1, No. 2, No. 3, No. 4, No. 5, No. 6, No. 7 and No. 8 are set up.

[0050] Preparation Example 1

[0051] Prepare deionized water, corn starch, and ammonium persulfate in a mass ratio of 100:17:1.5. Mix the deionized water, corn starch, and ammonium persulfate, and heat while stirring to gelatinize the starch at 95°C. After gelatinization, cool to 50°C and maintain this temperature. Adjust the pH of the gelatinized starch to 10 using a 10% sodium hydroxide solution. Then add dodecane bromo, with a mass ratio of dodecane bromo to corn starch of 1:1, and continue stirring for 4 hours. After the reaction, adjust the pH to neutral using a 1 mol / L hydrochloric acid solution to obtain the reaction slurry.

[0052] The reaction slurry was placed in 3 times its volume of anhydrous ethanol and allowed to stand for 1 hour. Then, it was filtered to obtain a filter cake. The filter cake was washed with ethanol and deionized water, dried at 70°C for 10 hours, and then pulverized to obtain dodecyl starch.

[0053] Preparation Example 2

[0054] Potassium dihydrogen phosphate, magnesium sulfate, ammonium chloride, and calcium chloride were dissolved in deionized water to prepare a treatment solution. The mass ratio of potassium dihydrogen phosphate, magnesium sulfate, ammonium chloride, calcium chloride, and deionized water was 3:1:2:2:10. 60-mesh perlite and the treatment solution were mixed at a mass ratio of 1:5, soaked for 1 hour, filtered, and dried to obtain pretreated perlite.

[0055] Dodecyl starch and deionized water were mixed and heated to 70°C with stirring to prepare a starch slurry with a mass fraction of 10%. The starch slurry was sprayed onto pretreated perlite with a mass ratio of starch slurry to pretreated perlite of 1:3. After drying, perlite coated with an alkylated starch film was obtained.

[0056] Preparation Example 3

[0057] Potassium dihydrogen phosphate, magnesium sulfate, ammonium chloride, and calcium chloride were dissolved in deionized water to prepare a treatment solution. The mass ratio of potassium dihydrogen phosphate, magnesium sulfate, ammonium chloride, calcium chloride, and deionized water was 3:1:2:2:10. 60-mesh perlite and the treatment solution were mixed at a mass ratio of 1:5, soaked for 1 hour, filtered, and dried to obtain pretreated perlite.

[0058] Dodecyl starch and deionized water were mixed and heated to 70°C with stirring to prepare a starch slurry with a mass fraction of 10%. Using a mass ratio of dodecyl starch to fulvic acid of 3:1, fulvic acid was added to the starch slurry. The starch slurry was then sprayed onto pretreated perlite at a mass ratio of 1:3. After drying, perlite coated with an alkylated starch film was obtained.

[0059] Preparation Example 4

[0060] Potassium dihydrogen phosphate, magnesium sulfate, ammonium chloride, and calcium chloride were dissolved in deionized water to prepare a treatment solution. The mass ratio of potassium dihydrogen phosphate, magnesium sulfate, ammonium chloride, calcium chloride, and deionized water was 3:1:2:2:10. 60-mesh vermiculite and the treatment solution were mixed at a mass ratio of 1:5, soaked for 1 hour, filtered, and dried to obtain pretreated vermiculite.

[0061] White dextrin and deionized water were mixed and heated to 50°C with stirring to prepare a 10% dextrin solution. The dextrin solution was sprayed onto vermiculite at a mass ratio of 1:3. After drying, vermiculite coated with a dextrin film was obtained.

[0062] Preparation Example 5

[0063] The difference between this preparation example and Preparation Example 4 is that dodecylated starch and deionized water were mixed, heated to 70°C, and stirred until homogeneous to prepare a starch slurry with a mass fraction of 10%. A starch slurry with a mass ratio of 1:2 to vermiculite coated with a dextrin membrane was sprayed onto the vermiculite coated with the dextrin membrane, and after drying, the treated vermiculite was obtained.

[0064] Preparation Example 6

[0065] Ammonium sulfate was dissolved in water to prepare an 8% ammonium sulfate solution. 50-mesh bamboo fiber was mixed with the ammonium sulfate solution at a mass ratio of 1:5, soaked for 1 hour, and then filtered and dried.

[0066] Preparation Example 7

[0067] Ammonium sulfate was dissolved in water to prepare an 8% ammonium sulfate solution. 50-mesh bamboo fiber was mixed with the ammonium sulfate solution at a mass ratio of 1:5, soaked for 1 hour, and then filtered and dried to obtain pretreated bamboo fiber.

[0068] Prepare fulvic acid by mixing ammonium sulfate and fulvic acid in a mass ratio of 1:1. Simultaneously prepare carboxymethyl cellulose (CMC) with a mass ratio of 1:1 to fulvic acid. Mix CMC, fulvic acid, and deionized water to prepare a coating solution with a CMC concentration of 3%. Spray the coating solution onto bamboo fibers with a mass ratio of 1:3 to the mass of the coating solution and bamboo fibers. After drying, bamboo fibers coated with a CMC membrane are obtained.

[0069] Example 1

[0070] Experimental site No. 1 was selected. The canopy closure of the Cathaya argyrophylla forest in Experimental Site No. 1 was 0.85. In March, thinning was carried out on the Cathaya argyrophylla forest to remove poorly growing trees and suppressed trees. After thinning, the canopy closure was 0.6.

[0071] After thinning, forest gaps are formed in the Cathaya argyrophylla forest. In March, the fallen leaves in the open spaces within the forest gaps are removed, and the soil is loosened manually to a depth of 2-3 cm. At the same time, bamboo fiber, as in Example 6, is mixed into the soil during the loosening process, with a mixing ratio of 3:20 between the bamboo fiber and the soil.

[0072] In August, weeding and soil preparation were carried out on the seedlings of Cathaya argyrophylla grown from seeds. The soil was deeply tilled to a depth of 10-20 cm, covering an area of ​​4 square meters. 2 Fertilizer was applied per plant during deep tilling, at a depth of 15 cm and an application rate of 100 g per plant. During deep tilling, perlite from Preparation Example 2 and vermiculite from Preparation Example 4 were mixed into the soil, with a perlite-to-soil mass ratio of 3:20 and a vermiculite-to-perlite mass ratio of 1:1.

[0073] Subsequently, the same deep tilling and topdressing operation should be carried out on the Cathaya argyrophylla seedlings once a year in August.

[0074] Example 2

[0075] Experimental site No. 2 was selected. The canopy closure of the Cathaya argyrophylla forest in Experimental site No. 2 was greater than 0.8. In March, thinning was carried out on the Cathaya argyrophylla forest to remove poorly growing trees and suppressed trees. After thinning, the canopy closure was 0.6.

[0076] After thinning, forest gaps are formed in the Cathaya argyrophylla forest. In March, the fallen leaves in the open spaces within the forest gaps are removed, and the soil is loosened manually to a depth of 2-3 cm. At the same time, bamboo fiber prepared in step 6 is mixed into the soil during the loosening process, with a mixing ratio of 3:20 between the bamboo fiber and the soil.

[0077] In August, weeding and soil preparation were carried out on the seedlings of Cathaya argyrophylla grown from seeds. The soil was deeply tilled to a depth of 10-20 cm, covering an area of ​​4 square meters. 2 Fertilizer was applied per plant during deep tilling, at a depth of 15 cm and an application rate of 100 g per plant. During deep tilling, perlite from Preparation Example 3 and vermiculite from Preparation Example 4 were mixed into the soil, with a perlite-to-soil mass ratio of 3:20 and a vermiculite-to-perlite mass ratio of 1:1.

[0078] Subsequently, the same deep tilling and topdressing operation should be carried out on the Cathaya argyrophylla seedlings once a year in August.

[0079] Example 3

[0080] Experimental site No. 3 was selected. The canopy closure of the Cathaya argyrophylla forest in Experimental site No. 3 exceeded 0.8. In March, thinning was carried out on the Cathaya argyrophylla forest to remove poorly growing trees and suppressed trees. After thinning, the canopy closure was 0.6.

[0081] After thinning, forest gaps are formed in the Cathaya argyrophylla forest. In March, the fallen leaves in the open spaces within the forest gaps are removed, and the soil is loosened manually to a depth of 2-3 cm. At the same time, bamboo fiber prepared in step 6 is mixed into the soil during the loosening process, with a mixing ratio of 3:20 between the bamboo fiber and the soil.

[0082] In August, weeding and soil preparation were carried out on the seedlings of Cathaya argyrophylla grown from seeds. The soil was deeply tilled to a depth of 10-20 cm, covering an area of ​​4 square meters. 2 Fertilizer was applied per plant during deep tilling, at a depth of 15 cm and an application rate of 100 g per plant. During deep tilling, perlite from Preparation Example 2 and vermiculite from Preparation Example 5 were mixed into the soil, with a perlite-to-soil mass ratio of 3:20 and a vermiculite-to-perlite mass ratio of 1:1.

[0083] Subsequently, the same deep tilling and topdressing operation should be carried out on the Cathaya argyrophylla seedlings once a year in August.

[0084] Example 4

[0085] Experimental site No. 4 was selected. The canopy closure of the Cathaya argyrophylla forest in Experimental site No. 4 exceeded 0.8. In March, thinning was carried out on the Cathaya argyrophylla forest to remove poorly growing trees and suppressed trees. After thinning, the canopy closure was 0.6.

[0086] After thinning, forest gaps are formed in the Cathaya argyrophylla forest. In March, the fallen leaves in the open spaces within the forest gaps are removed, and the soil is loosened manually to a depth of 2-3 cm. At the same time, bamboo fiber prepared in step 7 is mixed into the soil during the loosening process, with a mixing ratio of 3:20 between the bamboo fiber and the soil.

[0087] In August, weeding and soil preparation were carried out on the seedlings of Cathaya argyrophylla grown from seeds. The soil was deeply tilled to a depth of 10-20 cm, covering an area of ​​4 square meters. 2 Fertilizer was applied per plant during deep tilling, at a depth of 15 cm and an application rate of 100 g per plant. During deep tilling, perlite from Preparation Example 2 and vermiculite from Preparation Example 4 were mixed into the soil, with a perlite-to-soil mass ratio of 3:20 and a vermiculite-to-perlite mass ratio of 1:1.

[0088] Subsequently, the same deep tilling and topdressing operation should be carried out on the Cathaya argyrophylla seedlings once a year in August.

[0089] Comparative Example 1

[0090] Experimental site No. 5 was selected. The canopy closure of the Cathaya argyrophylla forest in Experimental site No. 5 exceeded 0.8. In March, thinning was carried out on the Cathaya argyrophylla forest to remove poorly growing trees and suppressed trees. After thinning, the canopy closure was 0.6.

[0091] After thinning, gaps are formed in the Cathaya argyrophylla forest. In March, the fallen leaves in the open spaces within the gaps are removed, and the soil is loosened manually to a depth of 2-3 cm.

[0092] In August, weeding and soil preparation were carried out on the seedlings of Cathaya argyrophylla grown from seeds. The soil was deeply tilled to a depth of 10-20 cm, covering an area of ​​4 square meters. 2 Fertilize each plant during deep tilling, at a depth of 15cm and a dosage of 100g per plant.

[0093] Subsequently, the same deep tilling and topdressing operation should be carried out on the Cathaya argyrophylla seedlings once a year in August.

[0094] Comparative Example 2

[0095] Experimental site No. 6 was selected. The canopy closure of the Cathaya argyrophylla forest at Experimental site No. 6 was 0.85. In March, thinning was carried out on the Cathaya argyrophylla forest to remove poorly growing trees and suppressed trees. After thinning, the canopy closure was 0.6.

[0096] After thinning, gaps are formed in the Cathaya argyrophylla forest. In March, the fallen leaves in the open spaces within the gaps are removed, and the soil is loosened manually to a depth of 2-3 cm.

[0097] In August, weeding and soil preparation were carried out on the seedlings of Cathaya argyrophylla grown from seeds. The soil was deeply tilled to a depth of 10-20 cm, covering an area of ​​4 square meters. 2 Fertilizer was applied per plant during deep tilling, at a depth of 15 cm and an application rate of 100 g per plant. During deep tilling, perlite from Preparation Example 2 and vermiculite from Preparation Example 4 were mixed into the soil, with a perlite-to-soil mass ratio of 3:20 and a vermiculite-to-perlite mass ratio of 1:1.

[0098] Subsequently, the same deep tilling and topdressing operation should be carried out on the Cathaya argyrophylla seedlings once a year in August.

[0099] Comparative Example 3

[0100] Experimental site No. 7 was selected. The canopy closure of the Cathaya argyrophylla forest at Experimental site No. 7 was 0.85. In March, thinning was carried out on the Cathaya argyrophylla forest to remove poorly growing trees and trees that were suppressed. After thinning, the canopy closure was 0.6.

[0101] After thinning, forest gaps are formed in the Cathaya argyrophylla forest. In March, the fallen leaves in the open spaces within the forest gaps are removed, and the soil is loosened manually to a depth of 2-3 cm. At the same time, bamboo fiber prepared in step 6 is mixed into the soil during the loosening process, with a mixing ratio of 3:20 between the bamboo fiber and the soil.

[0102] In August, weeding and soil preparation were carried out on the seedlings of Cathaya argyrophylla grown from seeds. The soil was deeply tilled to a depth of 10-20 cm, covering an area of ​​4 square meters. 2 Fertilizer is applied per plant during deep tilling, at a depth of 15 cm and an application rate of 100 g per plant. During deep tilling, perlite, as in Example 2, is mixed into the soil at a mass ratio of 3:20.

[0103] Subsequently, the same deep tilling and topdressing operation should be carried out on the Cathaya argyrophylla seedlings once a year in August.

[0104] Comparative Example 4

[0105] Experimental site No. 8 was selected. The canopy closure of the Cathaya argyrophylla forest at Experimental site No. 8 was 0.85. In March, thinning was carried out on the Cathaya argyrophylla forest to remove poorly growing trees and trees that were suppressed. After thinning, the canopy closure was 0.6.

[0106] After thinning, forest gaps are formed in the Cathaya argyrophylla forest. In March, the fallen leaves in the open spaces within the forest gaps are removed, and the soil is loosened manually to a depth of 2-3 cm. At the same time, bamboo fiber prepared in step 6 is mixed into the soil during the loosening process, with a mixing ratio of 3:20 between the bamboo fiber and the soil.

[0107] In August, weeding and soil preparation were carried out on the seedlings of Cathaya argyrophylla grown from seeds. The soil was deeply tilled to a depth of 10-20 cm, covering an area of ​​4 square meters. 2 Fertilizer was applied per plant during deep tilling, at a depth of 15 cm and an application rate of 100 g per plant. During deep tilling, vermiculite as in Example 4 was prepared into the soil, with a mixing mass ratio of vermiculite to soil of 3:20.

[0108] Subsequently, the same deep tilling and topdressing operation should be carried out on the Cathaya argyrophylla seedlings once a year in August.

[0109] For each embodiment and comparative example, the growth of Cathaya argyrophylla seedlings generated from seed germination was recorded for three consecutive years (October 2022, October 2023, and October 2024). The results are shown in Table 1, where h is the average height of the Cathaya argyrophylla seedlings and d is the average diameter at ground level of the Cathaya argyrophylla seedlings.

[0110] Table 1. Growth of Cathaya argyrophylla seedlings over three years

[0111]

[0112] As can be seen from Table 1, compared with the comparative example, the Cathaya argyrophylla seedlings in the embodiments of the present invention have better growth, indicating that by carrying out appropriate thinning of the Cathaya argyrophylla forest, and combining it with artificial land preparation and tending management, the mature seeds that fall naturally in the Cathaya argyrophylla forest can be promoted to germinate and grow, thereby achieving the purpose of renewing the Cathaya argyrophylla forest.

[0113] It will be apparent to those skilled in the art that this disclosure is not limited to the details of the exemplary embodiments described above, and that this disclosure can be implemented in other specific forms without departing from the spirit or essential characteristics of this disclosure. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of this disclosure is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this disclosure.

[0114] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A method for artificially promoting the natural regeneration of Cathaya argyrophylla, characterized in that, include: Forest thinning: Appropriately remove broad-leaved trees from the Cathaya argyrophylla community to create forest gaps around the mother trees and understory saplings and seedlings. After thinning, the canopy closure of the forest stand should be maintained at 0.5-0.

6. Manual land preparation: Clear and pile the fallen leaves in the open space inside the forest gap around the saplings of Cathaya argyrophylla, and loosen the soil in the open space to a depth of about 2-3 cm. Nurturing and management: Clear away weeds, shrubs, and harmful organisms around the saplings of Cathaya argyrophylla in the forest gaps that affect their growth, and cultivate the base of the trees and apply fertilizer. When tilling and loosening the soil, bamboo fiber is mixed into the soil. The bamboo fiber is first loaded with ammonium sulfate and then wrapped with a carboxymethyl cellulose membrane containing humic acid. The fertilization and top dressing operation involves deep tilling of the soil around the outside of the Cathaya argyrophylla tree to a depth of 10-20 cm, mixing perlite and vermiculite into the soil during the deep tilling process. The perlite is prepared by first loading a growth-promoting substance and then coating it with an alkylated starch film; the vermiculite is prepared by first loading a growth-promoting substance and then coating it with a dextrin film. The mass ratio of the perlite to the soil is (3-5):20, and the mass ratio of the vermiculite to the perlite is 1:(1-3).

2. The method for artificially promoting the natural regeneration of Cathaya argyrophylla according to claim 1, characterized in that, In the carboxymethyl cellulose membrane, the mass ratio of humic acid to carboxymethyl cellulose is 1:(1-2).

3. The method for artificially promoting the natural regeneration of Cathaya argyrophylla according to claim 1, characterized in that, The perlite has a mesh size of 60-80 mesh; and / or, the vermiculite has a mesh size of 60-80 mesh.

4. The method for artificially promoting the natural regeneration of Cathaya argyrophylla according to claim 1, characterized in that, The perlite is prepared by: making a nutrient solution from a growth-promoting substance, mixing the nutrient solution with perlite, soaking and filtering to obtain perlite loaded with the growth-promoting substance. Perlite loaded with growth-promoting substances and alkylated starch slurry are mixed, filtered, and dried to obtain perlite coated with an alkylated starch film.

5. The method for artificially promoting the natural regeneration of Cathaya argyrophylla according to claim 4, characterized in that, The alkylated starch slurry contains humic acid.

6. The method for artificially promoting the natural regeneration of Cathaya argyrophylla according to claim 1, characterized in that, The vermiculite is prepared by: making a nutrient solution from a growth-promoting substance, mixing the nutrient solution with vermiculite, soaking and filtering to obtain vermiculite loaded with the growth-promoting substance; Vermiculite loaded with growth-promoting substances and dextrin solution were mixed, filtered, and dried to obtain vermiculite coated with an alkylated starch film.

7. The method for artificially promoting the natural regeneration of Cathaya argyrophylla according to claim 6, characterized in that, The vermiculite is further coated with an alkylated starch film on the outside of the dextrin film.

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