Method for culturing in vitro embryos and regenerating plants of ormosia hosiei in western Hubei province

Abstract

The invention provides a key technology for culturing in vitro embryos and regenerating plants of ormosia hosiei in western Hubei province, belonging to the technologies of preserving and massively propagating an ormosia hosiei seed resource. The problems that the ormosia hosiei seeds are difficult to obtain and easily eaten by rats, since the seeds are hard, the dried seed husks can not absorb water easily due to poor water permeability, the natural propagation capability and the spreading capability are not strong, the fructification age is late, natural regeneration is difficult, blooming and fructification require 3-5 years, and good seedlings are scarce in the prior art are solved. According to the key technology, full seeds fruiting in the current year are selected as a material; after 20-35 days of embryo induction cultivation, robustly growing embryo plants can sprout up; after 25-45 days of multiplication cultivation, 20-35 days of strong seedling cultivation and 25-35 days of rooting cultivation, seedlings can be formed and then transplanted. The survival rate can achieve over 95%, the propagation coefficient is high, the propagation time is short, the survival rate of seedling transplanting is high, and the seedlings which are robust, tall and straight have bright green leaves and are excellent and uniform in growth.

Description

technical field [0001] The invention belongs to a cultivation method of an economic forest, in particular relates to a unique local tree species of red bean in western Hubei, and belongs to a tissue culture method for the preservation and mass propagation of the seed resources of the woody plant red bean. Background technique [0002] Ormosia hosiei.et Wils belongs to Papilionaceae family. It is a unique native species in my country and a national second-class rare and endangered plant. Red bean wood is currently the most important wood raw material in Chinese mahogany furniture. Red bean tree integrates precious materials, medicinal value, landscape utilization, and forest culture. It has extremely high economic value and development and utilization prospects. It is immortal and is known as "plant ruby". Its profound cultural background can be used as a "feng shui positioning" for courtyards. In Tang poetry, "red beans grow in the southern country, and a few branches wil...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a tissue culture method for isolated embryo culture and plant regeneration of red bean tree in western Hubei, which solves the problem that the seeds of red bean tree in the prior art are easily eaten by rats; the seeds are hard, and the seed coat is not easy to absorb water after drying; The water permeability of the skin is poor, and it must undergo special germination treatment before it can germinate. It is difficult to regenerate naturally, and the ability of natural reproduction and spread is not strong; and the age of fruiting is late (generally it takes about 35 years to flower and set, and the peak period of fruiting is after 50 years) and It takes 3 to 5 years to bloom and bear fruit, the source of seeds is difficult, and the problem of scarcity of good seedlings

Images