Quick propagation method of Tectona grandis
A golden wire and fast technology, applied in the field of plant cultivation, can solve the problems of limited mother tree resources, unsuitable seeds, tall trees, etc., and achieve the effects of low production cost of seedlings, good market application prospects, and convenient operation.
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Embodiment 1
[0024] Choose a good single plant with fast growth and straight trunk as the mother tree for collecting explants. In March of each year, collect the shoots of that year with a diameter of 0.5 to 1.0 cm and a length of about 10 cm. The branches must be free of pests and diseases. After that, it was transported to the tissue culture laboratory immediately, and then rinsed with tap water for 30 minutes, the leaves were removed, and the branches were cut into 5 cm long shoot tip explants. On the ultra-clean workbench in the inoculation room, rinse the explants with sterile water for 2 to 3 times, then disinfect the surface with 70% alcohol for 30 seconds, rinse with sterile water twice, and then place it in 0.2% Soak it in the mercury solution for 15-20 minutes (add 1 to 2 drops of Tween-80 to the solution). After taking it out, wash it with sterile water for 6-8 times. Then place the explant in a sterile petri dish, and use a sharp scalpel to cut the explant into a block with a lengt...
Embodiment 2
[0026] Repeat Example 1, with the following difference: the first stem segment below the shoot tip of the current year was collected as an explant. It is required to have obvious bud eyes, 1.5~2.0cm long and 1.0~1.5cm diameter.
Embodiment 3
[0028] Repeat Example 1, with the following differences: when the seedlings are tempered, the seedlings are directly transplanted into the nutrient soil to cultivate bare-rooted seedlings.
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