Licorice rapid propagation seedling culture method

A technology of licorice and seedling propagation, applied in horticultural methods, botany equipment and methods, horticulture, etc., to achieve the effects of fast growth rate, stable growth of seedlings, and high induction rate

Inactive Publication Date: 2012-06-20
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still few studies on the rapid propagation of licorice seedlings at present. This method also pro

Method used

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  • Licorice rapid propagation seedling culture method

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example 1

[0012] 1. With 1mol / L HCl and NaOH solution, adjust 100mL MS solid medium with 20g / L sucrose to pH 5.9, sterilize at 121°C and 0.1MPa pressure for 15 minutes for later use; licorice seeds (provided by Beijing Times) Provided by Zhen Institute of Traditional Chinese Medicine) Rinse with tap water and soak for 3 hours, soak in 75% ethanol for 10s in an ultra-clean workbench, rinse with sterile water for 2 to 3 times, then soak in 4% NaClO solution for 15min, and finally wash with sterile water Rinse with water 3 to 4 times and set aside. Then use sterilized tweezers to connect the seeds to the pre-prepared medium, and culture them under sterile, 25±1°C and dark conditions to induce germination. After 2 days of cultivation, the formation of buds can be seen with the naked eye, and after 3 or 4 days The buds are about 0.5cm long. Using 1mol / L HCl and NaOH solution, adjust 70mL MS solid medium with 20g / L sucrose in a 250mL Erlenmeyer flask to pH 5.9, and sterilize at 121°C and 0.1...

example 2

[0017] 1. With 1mol / L HCl and NaOH solution, adjust 100mL MS solid medium with 30g / L sucrose to pH 6.0, and sterilize at 121°C and 0.1MPa pressure for 15 minutes for later use; Glycyrrhizae seeds (purchased from Beijing Shizhen Institute of Traditional Chinese Medicine) rinsed with tap water and soaked for 3 hours, soaked the soaked seeds in 75% ethanol for 10 seconds in a clean bench, rinsed them with sterile water for 2-3 times, and then soaked them in 4% NaClO solution for 15 minutes , and finally rinsed with sterile water 3 to 4 times, set aside. Use sterilized tweezers to connect the seeds to MS solid medium, culture under sterile, 25±1°C and dark conditions, and induce germination. After 2 days of cultivation, the formation of buds can be seen with the naked eye. After 3 or 4 days, the buds grow 0.5 about cm. With 1mol / L HCl and NaOH solution, adjust 70mL MS solid medium with 30g / L sucrose in a 250mL Erlenmeyer flask to pH 6.0, and sterilize at 121°C and 0.1MPa pressure...

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Abstract

The invention relates to a licorice rapid propagation seedling tissue culture method. The method comprises steps of licorice aseptic seed germination culture, licorice aseptic seedling culture, licorice rapid propagation seedling induction and unrooted rapid propagation seedling rooting culture, the steps are respectively carried out in an MS solid medium containing 20-30g/L of sucrose under aseptic 24-26DEG C conditions, mediums for the rapid propagation seedling induction and the rapid propagation seedling rooting are added with a plant growth conditioning agent, and the pH of the MS solid medium before disinfection is adjusted to 5.9-6.0. The tissue culture method which is utilized in the invention to culture the licorice rapid propagation seedling has more practical meanings than cell culture and hairy root culture, and allows the growth rate to be rapid and the propagation multiple to be high to 6 times. The method of the invention, which allows the nursery stock growth to be stable and the culture to be uninfluenced by natural environments of natural disasters and the like, has the characteristics of simple process, high inductivity, good repeatability, high rapid propagation seedling increment multiple and the like.

Description

Technical field: [0001] The invention relates to a method for tissue culture of rapid propagation of licorice seedlings. Background technique: [0002] Glycyrrhiza licorice is the dry root and rhizome of Glycyrrhiza uralens Fisch., G. inflata Bat and G. glabra L. of leguminous plants. Modern pharmacological research shows that licorice has pharmacological effects such as anti-ulcer, anti-inflammatory, anti-allergic, antitussive and expectorant, and is one of the most commonly used traditional Chinese medicines. There has always been a saying of "ten prescriptions and nine herbs". Licorice is widely used in food, beverages, cigarettes, cosmetics, etc. In recent years, due to people's destructive digging of wild licorice, the licorice resources in our country have been severely damaged. For this reason, the country has protected wild clumps and vigorously advocated artificial planting of licorice. But due to the hard texture of licorice seeds. The seed coat is thick and har...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 高文远吕连媛张黎明王娟左北梅
Owner TIANJIN UNIV
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