Large-scale breeding method of Borneol Cinnamomum camphora

A production method and a technology of borneol camphor are applied in the field of plant reproduction, and can solve the problems of insufficient lignification degree, weak root system of rooting seedlings, low reproduction coefficient and the like

Active Publication Date: 2012-08-15
SUNSHINE HORTICULTURE
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0004] The invention provides a production method for the large-scale propagation of borneol camphor, which can effectively solve the problems of low reproduction coefficient of borneol camphor in tissue culture, weak root system of rooted seedlings, insufficient lignification degree, low survival rate of field planting, etc.

Method used

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Embodiment Construction

[0025] A kind of production method of borneol camphor camphor scaled propagation of the present invention specifically comprises the steps:

[0026] Step 1, mother plant selection, explant cultivation and pretreatment:

[0027]In spring, select the perennial borneol camphor that is excellent, strong, free from diseases and insect pests, and has stable and high average content (such as more than 82% D-borneol) as the original species, and intercepts the annual borneol camphor from the original species of borneol. The branches of the cuttings are used as the cutting female parent, and semi-lignified or lignified branches are cut from the cutting female parent as cuttings. Each cutting has 1 to 2 nodes. After being treated with 500ppm citric acid for 1 minute, transfer to After soaking in the sterile solution for 20 minutes, the cuttings are inserted into the matrix, which is formed by mixing peat soil with a thickness of 0-10mm and perlite in a ratio of 2:1.

[0028] After the ...

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Abstract

The invention relates to a large- scale breeding method of Borneol Cinnamomum camphora. The method comprises the following steps of: breeding a plantlet obtained through unique cuttage treatment by utilizing a current-growth lignified branch of strongly disease-resistant, no pest and disease damage and grown-up seed tree for about one year, acclimatizing, subculturing and propagating a sterile material after obtaining the sterile material with the current germinal sprout as an explant, secondly carrying out strong seeding rootage cultivation, and finally cultivating seeding in a greenhouse. According to the method disclosed by the invention, induced, propagated, strengthened and rooting culture mediums are optimized and improved, the stable growth of the sterile material can be effectively ensured, the sizes of sprouts are uniform, vitrification or defoliation of a tissue culture seedling can be avoided, the strong tissue culture seedlings can be obtained for sure, the repeatability is strong, an acclimation period and a subculture period are stable, a browning situation is reduced, a propagation coefficient is improved, the survival rate of planting in a greenhouse is improved, asubculture block mass cutting method determines the growth and the propagation coefficient of later-stage materials and can meet requirements of large-scale production.

Description

technical field [0001] The invention relates to the technical field of plant propagation, in particular to a production method for large-scale propagation of Cinnamomum camphora (L.) Sie. Background technique [0002] Borneo camphor is a plant of the genus Lauraceae, an evergreen tree. Originating in the Sumatra Islands of Indonesia, it is a precious medicinal spice tree species and landscaping tree species rich in aromatic oils, and is also an important chemical raw material and natural plant resource of borneol (D-borneol) (natural borneol is a natural plant resource) It is also an effective transdermal accelerator, which has anti-inflammatory, anti-myocardial ischemia, antibacterial, analgesic, and antiseptic effects) (Cheng Biqiang et al., 1997). For a long time, the development and utilization of borneol camphor has been mainly based on the extraction of essential oils. Over-harvesting has caused serious damage to its resources, especially the excellent tree resources ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01H4/00
Inventor 叶清梅李雪张天进黄雨花陈艳红陈柳红杨双霞
Owner SUNSHINE HORTICULTURE
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