67results about How to "Reduce vitrification" patented technology

The invention discloses a method for regenerating a sapium japonicum plant by inducing a sapium japonicum stem rapidly. The method for regenerating the sapium japonicum plant by inducing the sapium japonicum stem rapidly comprises the steps of explant culture, explant treatment, adventitious bud induction, adventitious bud subculture multiplication, adventitious bud rooting, bed arranging and matrix preparing, and domestication and transplantation. According to the method for rapidly regenerating the sapium japonicum plant by inducing the sapium japonicum stem, water planting and seedling regenerating are conducted to a sapium japonicum branch through a self-prepared nutrient solution, a new twig can be obtained to serve as an explant, the contamination rate of the explant can be controlled to be lower than 5% according to the magnetic stirring type sterilization method, and the contamination rate of the explant is lowered by 15% compared with a conventional treatment method. According to the method for regenerating the sapium japonicum plant by inducing the sapium japonicum stem rapidly, the culture cycle is short, the seedlings are germinated rapidly and are uniform, the culture cost is low, the culture process is simple, the contamination rate of sapium japonicum explants is lower than 5%, the adventitious bud induction rate reaches above 85%, the multiplication coefficient reaches 8.0, the vitrification rate is lowered by 5.5%, the adventitious bud rooting rate reaches 95%, and the survival rate of transplanting reaches above 95%, the requirement for large-scale culture of sapium japonicum can be effectively met, and planting of the sapium japonicum is promoted.

The invention discloses an industrial breeding method for the detoxified group cultured seedlings of bamboo root ginger, which comprises four steps of induction of clustered buds at the shoot tip, subculture of the clustered buds, rooting culture and transplanting of test-tube seedlings. The culture medium, rooting medium and transplanting matrix were optimized, and the obtained method had a high induction rate of clustered buds at the shoot tip; the multiplicity of the clustered shoots was high and the vitrification rate was low; the rooting rate of the sprouts reached 100%, and the root system was strong and vitrified. The lateral buds germinate; the transplanting survival rate is high, and the sprouts are strong and grow quickly; the method of the invention has short cycle and low cost, can fully meet the needs of large-scale cultivation of bamboo root ginger, and has good application prospects.

The invention belongs to the field of plant regeneration, and provides a method for overcoming the vitrification of carnation stem tip cultivation by using high-molecular water-absorbing resin, comprising the steps of: 1) selecting a robust carnation plant, and cutting off a 2 cm stem segment with a top; 2) Rinse with sterile water 3-5 times and set aside; 3) Peel off 0.5mm of carnation stem tip and insert it into the culture medium, the culture medium is: iron salt doubled MS+sucrose 30g / L+agar 6g / L+TDZ1g / L +NAA1g / L+8g / L polymer water-absorbent resin, and then cultivated at 20-30°C. The present invention determines the selection of medium additive species through tests, and selects the concentration of the additives through test comparison. According to the test results, the polymer water-absorbing resin with suitable concentration can reduce the incidence of vitrification to about 17% after the detoxification of the stem tip of carnation red variety, and the subsequent vitrification of the non-vitrified plants is also greatly reduced. reduce.