67results about How to "Reduce vitrification" patented technology

The invention discloses a method for regenerating a sapium japonicum plant by inducing a sapium japonicum stem rapidly. The method for regenerating the sapium japonicum plant by inducing the sapium japonicum stem rapidly comprises the steps of explant culture, explant treatment, adventitious bud induction, adventitious bud subculture multiplication, adventitious bud rooting, bed arranging and matrix preparing, and domestication and transplantation. According to the method for rapidly regenerating the sapium japonicum plant by inducing the sapium japonicum stem, water planting and seedling regenerating are conducted to a sapium japonicum branch through a self-prepared nutrient solution, a new twig can be obtained to serve as an explant, the contamination rate of the explant can be controlled to be lower than 5% according to the magnetic stirring type sterilization method, and the contamination rate of the explant is lowered by 15% compared with a conventional treatment method. According to the method for regenerating the sapium japonicum plant by inducing the sapium japonicum stem rapidly, the culture cycle is short, the seedlings are germinated rapidly and are uniform, the culture cost is low, the culture process is simple, the contamination rate of sapium japonicum explants is lower than 5%, the adventitious bud induction rate reaches above 85%, the multiplication coefficient reaches 8.0, the vitrification rate is lowered by 5.5%, the adventitious bud rooting rate reaches 95%, and the survival rate of transplanting reaches above 95%, the requirement for large-scale culture of sapium japonicum can be effectively met, and planting of the sapium japonicum is promoted.

The present invention relates to a process for production of a synthetic quartz glass having a fluorine concentration of 1,000 mass ppm or more, the process comprising: (a) a step of depositing and growing quartz glass fine particles obtained by flame hydrolysis of a glass forming raw material onto a substrate, to thereby form a porous glass body; (b) a step of keeping the porous glass body in a reaction vessel that is filled with elemental fluorine (F2) or a mixed gas comprising elemental fluorine (F2) diluted with an inert gas and contains a solid metal fluoride, to thereby obtain a fluorine-containing porous glass body; and (c) a step of heating the fluorine-containing porous glass body to a transparent vitrification temperature, to thereby obtain a fluorine-containing transparent glass body.

The invention discloses a daphne genkwa stem explant primary tissue culture method which comprises the following steps: (1) selecting a daphne genkwa explant: acquiring a daphne genkwa annual branch, removing all leaves and cutting the branch into axillary bud stems as the explant, (2) treating the daphne genkwa explant: soaking and washing the explant with a washing powder solution, rinsing the explant with running water, performing disinfection treatment and then shifting the explant into a culture medium of daphne genkwa stem explant primary tissue culture, and (3) culturing the daphne genkwa explant: performing irradiation culture on the explant shifted into the primary culture medium in a culture rack of a culture room until inducted clustered buds germinate. According to the method, the stem explant induced clustered buds are adopted, so that a daphne genkwa tissue culture seedling can be simply and quickly obtained, a culture period is obviously shortened, a culture success rate is obviously increased, a germination rate is high, and a vitrification rate is low.

The invention discloses a primary fir wood culture bud induction method. The method comprises the following steps: inoculating an explant as a coppice shoot of a fir wood base part and subjected to sterile explant treatment to a bud induction culture medium subjected to high-quality primary culture, culturing under the conditions of proper temperature, humidity and illumination intensity, and inducing bud germination. The conventional fir wood induction technology is improved, the problems that the explant is subjected to browning and vitrifaction in the fir wood bud induction process and the like are solved, the fir wood bud induction rate, the budding index and the germination and growth conditions are improved, and large-number high-quality buds are obtained, so that the multiplication culture requirement is met, the establishment of a rapid fir wood tissue culture reproductive system is promoted, and the method has high economic benefits and social benefits.

The invention discloses an industrial breeding method for the detoxified group cultured seedlings of bamboo root ginger, which comprises four steps of induction of clustered buds at the shoot tip, subculture of the clustered buds, rooting culture and transplanting of test-tube seedlings. The culture medium, rooting medium and transplanting matrix were optimized, and the obtained method had a high induction rate of clustered buds at the shoot tip; the multiplicity of the clustered shoots was high and the vitrification rate was low; the rooting rate of the sprouts reached 100%, and the root system was strong and vitrified. The lateral buds germinate; the transplanting survival rate is high, and the sprouts are strong and grow quickly; the method of the invention has short cycle and low cost, can fully meet the needs of large-scale cultivation of bamboo root ginger, and has good application prospects.

The invention belongs to the field of plant regeneration, and provides a method for overcoming the vitrification of carnation stem tip cultivation by using high-molecular water-absorbing resin, comprising the steps of: 1) selecting a robust carnation plant, and cutting off a 2 cm stem segment with a top; 2) Rinse with sterile water 3-5 times and set aside; 3) Peel off 0.5mm of carnation stem tip and insert it into the culture medium, the culture medium is: iron salt doubled MS+sucrose 30g / L+agar 6g / L+TDZ1g / L +NAA1g / L+8g / L polymer water-absorbent resin, and then cultivated at 20-30°C. The present invention determines the selection of medium additive species through tests, and selects the concentration of the additives through test comparison. According to the test results, the polymer water-absorbing resin with suitable concentration can reduce the incidence of vitrification to about 17% after the detoxification of the stem tip of carnation red variety, and the subsequent vitrification of the non-vitrified plants is also greatly reduced. reduce.

The invention discloses a method for inducing and multiplying adventitious buds of Cyclocarya paliurus, belonging to the technical field of plant tissue culture. Select the young shoots of Cyclocarya paliurus as explants, before the explants are induced and value-added culture, the explants are first sterilized and disinfected; the sterilized treatment adopts alcohol treatment for 25-30s, sodium hypochlorite treatment for 3-4min; Adventitious bud induction medium formula: WPM+6‑BA+sucrose+agar powder, and adjust pH to 5.75‑5.85; Cyclocarya adventitious bud proliferation medium formula: WPM+IBA+sucrose+agar powder. The present invention obtains a large amount of adventitious bud materials with strong growth, stretched leaves and high growth in a relatively short period of time by optimizing materials, sterilizing treatment and cultivating formula, laying the foundation for establishing an efficient in vitro rapid propagation system of Cyclocarya paliurus, and also It is of great significance to the protection, development and utilization of Cyclocarya paliurus germplasm resources.