67results about How to "Reduce vitrification" patented technology

The invention belongs to the technical field of porcelain glaze of enamel, and particularly relates to a formula of cracking preventing porcelain glaze of enamel and preparation of the cracking preventing porcelain glaze of the enamel. After the cracking preventing porcelain glaze is coated on an enamel steel plate with porcelain about to be cracked and is baked, an enamel product comprising the cracking preventing porcelain glaze cannot be cracked. The cracking preventing porcelain glaze comprises SiO2, B2O3, Al2O3, Na2O, K2O, Li2O, CaO, TiO2, MgO, BaO, Co2O3, NiO, Fe2O3, MnO2 and CuO. After the components are uniformly mixed, uniformity of mixture of the components is larger than or equal to 98%. After the mixture is smelted in a smelting furnace at the temperature of 12200C-12800 DEG C, glass fibers are drawn, are discharged when each mile of the glass wires has 2-3 joints, and then are cooled and crushed. After the cracking preventing porcelain glaze is coated on the enamel steel plate with the porcelain about to be cracked, the enamel product comprising the cracking preventing porcelain glaze cannot be cracked.

The invention discloses a primary fir wood culture bud induction method. The method comprises the following steps: inoculating an explant as a coppice shoot of a fir wood base part and subjected to sterile explant treatment to a bud induction culture medium subjected to high-quality primary culture, culturing under the conditions of proper temperature, humidity and illumination intensity, and inducing bud germination. The conventional fir wood induction technology is improved, the problems that the explant is subjected to browning and vitrifaction in the fir wood bud induction process and the like are solved, the fir wood bud induction rate, the budding index and the germination and growth conditions are improved, and large-number high-quality buds are obtained, so that the multiplication culture requirement is met, the establishment of a rapid fir wood tissue culture reproductive system is promoted, and the method has high economic benefits and social benefits.

The present invention uses the dormand bud to be activated in early spring as explant and adopts the following steps: sterilizing, removing bud scale, cutting off small leaflet in the bud, inoculating residual portion on the different culture media to make culture, then regularly making subculture so as to implement propagation of lots of tissue-cultured seedlings and prevent the production of browning and vitrifiation.

The invention discloses a Zhugen ginger detoxification tissue culture seedling industrialized breeding method, which comprises four steps of induction of shoot-tip clustered shoots, subculture multiplication of the clustered shoots, rooting culture and transplantation of test-tube seedlings and respectively optimizes an induction medium, a multiplication medium, a rooting medium, a transplantation medium and the like. The method has high shoot-tip clustered shoot induction rate, high multiple of subculture multiplication of the clustered shoots and low vitrification ratio; the seedling rooting rate reaches 100 percent; the root system is robust and lateral buds sprout; the transplanting survival rate is high and the seedlings are robust and grow quickly; and the method of the invention has short period and low cost, can completely meet the requirements of large-area scale culture of Zhugen ginger and has good application prospect.

The invention relates to a paraserianthes falcataria clone tissue culture method. According to the method, aseptic seedlings obtained from paraserianthes falcataria excellent mother tree seeds with an aseptic seedling-raising technology are adopted as explants, or paraserianthes falcataria excellent mother tree budding shoots are adopted as explants; the explants are subjected to induction culture, proliferation culture, rooting culture, and seedling hardening, and are transplanted into and cultured in a seedling-raising medium. According to the method, technical links such as the induced proliferation process, culture media and culture conditions, and seedling hardening are optimized. Cytokinin 6-BA is added into an induction medium; during proliferation culture, tissue culture seedlings are subjected to natural scattering light culture for 28-35 days, such that rooting and seedling hardening are combined; plant transplanting is carried out at later than 4:30pm on sunny days or on cloudy days; and the components of various culture media are optimized, such that seedling vitrification phenomenon, yellowing phenomenon and ineffective callus generation are effectively reduced, proliferation rate and seedling emergence rate are improved, a culture period is shortened, and seedling survival rate can be higher than 90%.

The invention discloses a method and device for disposing waste incineration fly ash in a low-energy-consumption, recycling and environment-friendly manner. The method comprises the following steps that: 1) 1480-1510 DEG C blast furnace slag is added into a melting furnace, fly ash is simultaneously or subsequently added, and an obtained mixture is fully stirred, and the addition amount of the flyash accounts for not more than 25% of the total mass of the blast furnace slag and fly ash; 2) the temperature of the composite slag in the melting furnace is kept at 1400-1450 DEG C, and the reaction time is kept for 20-40 minutes under the temperature; and 3) deslagging, cooling, and water quenching treatment are carried out to obtain powdery water granulated slag, and the powdery water granulated slag is used as cement aggregate after being subjected to fine grinding.

The invention discloses a culture medium box set for facilitating crgotvaunilocularis in-vitro rapid propagation. The culture medium box set comprises a culture medium 1 for primary culture, a culture medium 2 for secondary culture, a culture medium 3 for toughening seedlings and a culture medium 4 for taking roots, wherein the culture medium 1 for primary culture takes an MS (murashige and skoog) culture medium as a basal culture medium, 0.5-2mg/L of BA (butyl acrylate), 0.1mg/L of NAA (naphthalene acetic acid), 0-0.1mg/L of TDZ (thidiazuron), 25-35mg/L of sucrose and 6.5-7.5 mg/L of agar are added into the culture medium, the culture medium 2 for secondary culture takes an MS culture medium as a basal culture medium, 0.5mg/L of BA, 0-0.1mg/L of NAA, 0-0.5mg/L of GA3 (gibberellin), 25-35mg/L of sucrose and 6.5-7.5 mg/L of agar are added into the culture medium, the culture medium 3 for toughening seedlings takes an MS culture medium as a basal culture medium, 25-35mg/L of sucrose and 6.5-7.5 mg/L of agar are added into the culture medium, the culture medium 4 for taking roots takes 1/2 MS culture medium as a basal culture medium, and 0.5-1.5 mg/L of IBA (indole-butyric acid), 25-35mg/L of sucrose and 6.5-7.5 mg/L of agar are added into the culture medium. The invention further discloses a method for facilitating crgotvaunilocularis in-vitro rapid propagation by the culture medium box set. Crgotvaunilocularis is cultured by the method under optimized conditions, and ideal reproduction rate, rooting rate and transplanting survival rate can be achieved.

The invention discloses a daphne genkwa stem explant primary tissue culture method which comprises the following steps: (1) selecting a daphne genkwa explant: acquiring a daphne genkwa annual branch, removing all leaves and cutting the branch into axillary bud stems as the explant, (2) treating the daphne genkwa explant: soaking and washing the explant with a washing powder solution, rinsing the explant with running water, performing disinfection treatment and then shifting the explant into a culture medium of daphne genkwa stem explant primary tissue culture, and (3) culturing the daphne genkwa explant: performing irradiation culture on the explant shifted into the primary culture medium in a culture rack of a culture room until inducted clustered buds germinate. According to the method, the stem explant induced clustered buds are adopted, so that a daphne genkwa tissue culture seedling can be simply and quickly obtained, a culture period is obviously shortened, a culture success rate is obviously increased, a germination rate is high, and a vitrification rate is low.

The invention relates to the technical field of agriculture and discloses a culture medium and sowing method for promoting seed germination of dendrobium huoshanense on the basis of existing problemsthat the seed germination rate of dendrobium huoshanense is low, the cost is high, and the quality needs to be improved. The culture medium is a solid culture medium containing 900-1,100 mg/L of potassium nitrate, 1,100-1,300 mg/L of ammonium nitrate, 225-275 mg/L of magnesium sulfate, 225-275 mg/L of potassium dihydrogen phosphate, 180-220 mg/L of calcium nitrate, 50-80 g/L of potatoes and 20-50g/L of bananas. The method for promoting the seed germination includes the steps of (1) seed treatment, (2) seed disinfection, (3) inoculation and (4) culture. The culture medium and the method have the advantages that the seed germination rate is increased from 50-60% to 90% or above, the time required for the seed germination is shortened, and meanwhile the lesion probability of seeds during germination is reduced.

The invention provides a Vitis amurensis Rupr. tissue culture medium. In the culture medium, the water potential of the culture medium is reduced by increasing the concentrations of substances including agar, sucrose and the like in the culture medium, and thus a Vitis amurensis Rupr. tissue culture seedling vitrification phenomenon, particularly a vitrification phenomenon at an induction phase, is alleviated to a certain extent. Meanwhile, active carbon is added into the culture medium so that a tissue culture seedling browning phenomenon caused by the fact that the concentration of the sucrose in the culture medium is increased can be avoided, and furthermore, the success rate of Vitis amurensis Rupr. tissue culture is improved. Meanwhile, the invention further provides a Vitis amurensis Rupr. tissue culture method. According to the method provided by the invention, a transition culture step is further added to an existing seedling tissue culture process, and the culture medium provided by the invention is used as an induction culture medium and a multiplication culture medium, so that the vitrification phenomenon in a Vitis amurensis Rupr. induction culture process and a Vitis amurensis Rupr. multiplication culture process is effectively controlled by optimizing a culture flow and the used culture medium.

The invention discloses a melon regeneration in vitro method. The melon regeneration in vitro method provided by the invention comprises the following steps: (1) taking a cotyledon of a melon as an explant, inoculating the explant into a shoot induction medium for culturing to obtain an adventitious bud tussock; (2) placing the adventitious bud tussock obtained in the step (1) to a shoot elongation medium A for culturing to obtain an adventitious bud; (3) placing the adventitious bud obtained in the step (2) into a rooting medium for culturing to obtain a melon regeneration seedling. The melon regeneration in vitro method provided by the invention has the advantages of high adventitious bud inductivity, low vitrification degree, short regeneration period and the like, and by applying the method in the melon genetic transformation, a relatively high transformation rate can be obtained.

The invention discloses a tissue culture breeding method for hippeastrum hippeastrum. The method comprises the following steps: (a) supply of explants: taking scales with bulb discs at the bases as the explants; (b) adventitious bud induction: transferring the disinfected explants into an induction culture medium, and culturing the explants for 25-30 days to obtain adventitious bud seedlings; (c) multiplication culture: c1, carrying out subculture on the adventitious bud seedlings in a solid culture medium; c2, after culturing for 3-4 weeks, cutting seed balls obtained in the step c1, and transferring cut bodies into a semi-solid culture medium for culturing; c3, after culturing for 3-4 weeks, cutting the seed balls obtained in the step c2, and transferring cut bodies into the solid culture medium; wherein the solid culture medium is prepared by adding 2.0 mg/L to 2.5 mg/L of 6-BA, 25 g/L to 35 g/L of cane sugar and 5.5 g/L to 6.5 g/L of carrageenan into an MS (Murashige and Skoog) culture medium, and the semi-solid culture medium is prepared by adding 0.8 mg/L to 1.2 mg/L of 6-BA, 25 g/L to 35 g/L of cane sugar and 2.5 g/L to 3.5 g/L of carrageenan into a 1/2 MS culture medium; and (d) transplanting of seedlings: transplanting the seedlings obtained by multiplication culture into a matrix for culture.

The invention discloses a special dendrobium officinale leaf sheath tissue culture seedling forming method, which comprises the following four steps of 1, selecting explants; 2, processing the explants; 3, preparing a seedling forming culture medium; 4, performing seedling domestication and planting. The method has the advantages that the leaf sheaths of dendrobium officinale are used for tissue culture for obtaining callus tissues, so that the callus rate is improved; the vitrification is reduced; the adventitious bud differentiation rate reaches 62.67 percent; each callus tissue block can form a plurality of adventitious buds; the rooting rate can be effectively ensured; the reproductive speed is greatly improved; the reproductive quantity is greatly increased; the nutrition ingredients of the cultured dendrobium officinale are richer than the nutrition ingredients of the dendrobium officinale in the market; the yield and the quality are improved.

The invention relates to the field of medicinal plant tissue culture and traditional Chinese medicinal materials, in particular to a culture medium for improving the domestication survival rate of euphorbia hirsuta tissue culture seedlings and a domestication method of the euphorbia hirsuta tissue culture seedlings. The culture method comprises the steps of explant disinfection, cluster bud induction, cluster bud proliferation, cluster bud rooting and rooting seedling domestication. According to the method, during rooting, low-concentration 2, 4-D and IBA are matched, rooting of euphorbia pekinensis can be effectively promoted, the rooting rate can be close to 100%, meanwhile, 2.0-2.4 mu g/L of silver nitrate and 0.2-0.3 mg/L of MET are added into a rooting bottle culture medium, the vitrification phenomenon of rooted seedlings can be completely inhibited, and the domestication survival rate can be increased to a great extent.

The invention discloses a primary tissue culture medium for a daphne genkwa stem explant. The primary tissue culture medium contains the following components: MS, 0.1mg.L<-1> of NAA and 1.0mg.L<-1> of ZT, 25-30g/L of saccharose and 6.5-7g/L of agar are added, and the pH is adjusted to be 5.7-5.8. The method for culturing primary tissue of the daphne genkwa stem explant by using the primary tissue culture medium comprises the steps of selection of daphne genkwa explant; treatment of the daphne genkwa explant; and culture of the daphne genkwa explant. Primary tissue culture is carried out on the daphne genkwa stem explant by using the primary tissue culture medium and cluster buds are induced, so that daphne genkwa tissue culture seedlings can be simply and quickly obtained, the culture period is obviously shortened, the culture success rate is obviously increased and the primary tissue culture medium is high in germination rate and low in vitrification rate.

The invention discloses an industrialized seedling culture method for detoxified health sugarcane seedlings. According to the industrialized seedling culture method, a sealing cover with ventilating and bacteria filtering functions is utilized for replacing conventional closed covers to seal a culture container in each indoor seedling culture stage of the detoxified health sugarcane seedlings; and by virtue of the ventilating sealing cover, the culture container has certain permeability, so that culture conditions from the establishing of sterile explants to rooting and acclimatization stages are regulated and controlled, the photoautotrophical capacity of cultures is remarkably improved, and the quality and the seedling culture efficiency of tissue cultured seedlings are improved. Practices show that by virtue of the industrialized seedling culture method, the vitrification rate of explants can be decreased in an induction stage of the explants; in multiplication and expanding propagation stages, the proportion of vitreous buds can be reduced, and the quality of cultured seedlings is improved; in a rooting stage, the induced rooting time can be shortened, and the rooting rate is increased; and in acclimatization and transplant stages, the seedling domestication period is shortened, and the survival rate of transplanted seedlings is increased. Therefore, the industrialized production efficiency of the detoxified health sugarcane seedlings is finally integrally improved, and the production cost of cultured seedlings is lowered.

The invention discloses a preparation method of a peony callus extract with high paeoniflorin content. The preparation method comprises the following steps: S1, preparing a peony explant: a, taking peony seeds, soaking the peony seeds with water, and covering the peony seeds with gauze until cracks appear on the surfaces of the seeds to obtain products A; b, washing the products A, then covering the products A with gauze, and disinfecting and degerming the products A twice after washing to obtain products B; c, transferring the products B into a germination culture medium for culturing until the seeds germinate seedlings to obtain products C; S2, induction of peony callus: putting the products C into an induction culture medium for callus induction culture to obtain products D; S3, culture of subculture peony callus: transferring the products D into a subculture medium for amplification culture, and carrying out subculture on schedule to obtain products E; and S4, preparation of the extract: preparing the products E into the peony callus extract to obtain a finished product. The preparation method has the characteristics of high inductivity, low browning and vitrification rate and high active substance content.