Special dendrobium officinale leaf sheath tissue culture seedling forming method

Abstract

The invention discloses a special dendrobium officinale leaf sheath tissue culture seedling forming method, which comprises the following four steps of 1, selecting explants; 2, processing the explants; 3, preparing a seedling forming culture medium; 4, performing seedling domestication and planting. The method has the advantages that the leaf sheaths of dendrobium officinale are used for tissue culture for obtaining callus tissues, so that the callus rate is improved; the vitrification is reduced; the adventitious bud differentiation rate reaches 62.67 percent; each callus tissue block can form a plurality of adventitious buds; the rooting rate can be effectively ensured; the reproductive speed is greatly improved; the reproductive quantity is greatly increased; the nutrition ingredients of the cultured dendrobium officinale are richer than the nutrition ingredients of the dendrobium officinale in the market; the yield and the quality are improved.

Description

technical field [0001] The invention relates to a method for forming seedlings through tissue culture of special dendrobium candidum leaf sheaths. Background technique [0002] Dendrobium candidum, an endangered plant, is also a good traditional Chinese medicine. It has the effects of benefiting the stomach, nourishing yin and clearing heat, and nourishing health. The development of Dendrobium candidum can well promote the culture of traditional Chinese medicine. It is demanding and likes to grow in a warm, humid, half-shady and half-sun environment, but it cannot be cultivated in ordinary soil. It mostly attaches to large tree trunks and rock crevices, and has a long growth cycle, which takes about five or six years. . Dendrobium has many flowers and few fruits, and the seeds are as fine as dust, each weighing about 0.3-0.4 μg, so it is not easy to collect for large-scale development, while the development of seed embryos cannot exceed the spherical stage, lacks endosperm ...

AI Technical Summary

Problems solved by technology

[0002] Dendrobium candidum, an endangered plant, is also a good traditional Chinese medicine. It has the effects of benefiting the stomach, nourishing yin and clearing heat, and nourishing health. The development of Dendrobium candidum can well promote the culture of traditional Chinese medicine. It is demanding and likes to grow in a warm, humid, half-shady and half-sun environment, but it cannot be cultivated in ordinary soil. It mostly attaches to large tree trunks and rock crevices, and has a long growth cycle, which takes about five or six years.

Dendrobium has many flowers and few fruits, and the seeds are as fine as dust, each weighing about 0.3-0.4 μg, so it is not easy to collect for large-scale development, while the development of seed embryos cannot exceed the spherical stage, lacks endosperm tissue, and requires symbiosis with fungi to germinate. sexual reproductive difficulties