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Primary tissue culture medium for daphne genkwa stem explant

A technique of tissue culture medium and tissue culture, which is applied in plant regeneration, botany equipment and methods, horticulture, etc., can solve the problems that the rapid propagation technology of stem explant tissue culture has not been reported, and achieves convenient and easy test operation and disinfection The effect of low fatality rate and small pollution rate

Inactive Publication Date: 2017-08-15
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research on Daphne genkwa mainly focuses on its chemical components and pharmacological effects, as well as the research on biological pesticides. There are few related researches on reproduction and cultivation techniques, and the rapid propagation technology of its stem explant tissue culture has not been seen at home and abroad. to report

Method used

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  • Primary tissue culture medium for daphne genkwa stem explant
  • Primary tissue culture medium for daphne genkwa stem explant
  • Primary tissue culture medium for daphne genkwa stem explant

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Primary tissue culture medium: MS+0.1mg·L -1 NAA+1.0mg·L -1 ZT, add 30g / L sucrose and 7g / L agar to adjust the pH to pH=5.8.

[0037] Training method:

[0038] (1) Selection of Daphne genkwa explants: collection of robust growth, full axillary buds, no pests and diseases of Daphne genkwa explants in the same year, remove all leaves, cut into 10cm stems with axillary buds as explants;

[0039] (2) Treatment of Daphne genkwa explants: soak the explants in a 0.5% washing powder aqueous solution for 20 minutes with a mass-volume ratio, then rinse them with running water for 12 hours, and place the explants under the aseptic environment of the ultra-clean workbench in the inoculation room. The body was cut into 4cm stem segments, sterilized with 75% ethanol aqueous solution for 30s, and then 1g·L -1Soak in a mixed solution of mercuric chloride and 5 drops of Tween 80 for 12 minutes for disinfection; rinse with sterile water after disinfection, cut the stem section into a l...

Embodiment 2

[0042] Primary tissue culture medium: MS+0.1mg·L -1 NAA+1.5mg·L -1 ZT, add 30g / L sucrose and 7g / L agar to adjust the pH to pH=5.8.

[0043] Training method:

[0044] (1) Selection of Daphne genkwa explants: collection of robust growth, full axillary buds, no pests and diseases of Daphne genkwa explants in the same year, remove all leaves, cut into 10cm stems with axillary buds as explants;

[0045] (2) Treatment of Daphne genkwa explants: soak the explants in 0.5% washing powder aqueous solution for 20 minutes, then rinse them with running water for 12 hours, and place the explants in the aseptic environment of the ultra-clean workbench in the inoculation room. Cut into 4cm stem segments, sterilize with 75% ethanol aqueous solution for 30s, and then use 1g·L -1 Soak in a mixed solution of mercuric chloride and 5 drops of Tween 80 for 12 minutes for disinfection; rinse with sterile water after disinfection, cut the stem section into a length of 2 cm, the upper cut of the ste...

Embodiment 3

[0048] Primary tissue culture medium: MS+0.1mg·L -1 NAA+1.5mg·L -1 ZT, add 25g / L sucrose and 6.5g / L agar to adjust the pH to pH=5.7.

[0049] Training method:

[0050] (1) Selection of explants of Daphne genkwa: collection of strong growth, full axillary buds, no pests and diseases of Daphne genkwa explants in the same year, remove all leaves, cut into 8cm stems with axillary buds as explants;

[0051] (2) Treatment of Daphne genkwa explants: Soak the explants in a 0.2% washing powder aqueous solution for 20 minutes, then rinse them with running water for 12 hours, and inoculate the explants under the aseptic environment of the ultra-clean workbench in the inoculation room. The body was cut into 5cm stem segments, sterilized with 70% ethanol aqueous solution for 45s, and then 1g·L -1 Soak in a mixed solution of mercuric chloride and 3 drops of Tween 80 for 10 minutes for disinfection; rinse with sterile water after disinfection, cut the stem section into a length of 2.5 cm,...

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Abstract

The invention discloses a primary tissue culture medium for a daphne genkwa stem explant. The primary tissue culture medium contains the following components: MS, 0.1mg.L<-1> of NAA and 1.0mg.L<-1> of ZT, 25-30g / L of saccharose and 6.5-7g / L of agar are added, and the pH is adjusted to be 5.7-5.8. The method for culturing primary tissue of the daphne genkwa stem explant by using the primary tissue culture medium comprises the steps of selection of daphne genkwa explant; treatment of the daphne genkwa explant; and culture of the daphne genkwa explant. Primary tissue culture is carried out on the daphne genkwa stem explant by using the primary tissue culture medium and cluster buds are induced, so that daphne genkwa tissue culture seedlings can be simply and quickly obtained, the culture period is obviously shortened, the culture success rate is obviously increased and the primary tissue culture medium is high in germination rate and low in vitrification rate.

Description

technical field [0001] The invention relates to primary plant tissue culture, in particular to a primary tissue culture medium for genkwa stem segment explants. Background technique [0002] Genkwa (Daphne genkwa Sieb.et Zucc.) is a deciduous shrub of Daphneaceae Daphne. Its flowers are clustered and colorful, and have high ornamental value. It is an excellent wild flower resource in my country. It is also an important Chinese herbal medicine and biological pesticide in my country. [0003] Since the 1960s, especially since the 1990s, the habitat of Daphne genkwa has been severely damaged, the distribution range of the population has shrunk, the number of populations and individuals has declined sharply, and its wild resources have gradually decreased. It is an urgent requirement to protect and utilize this plant resource to study the reproduction technology of Daphne genkwa. [0004] Daphne genkwa is generally propagated by sowing, but the traits of the offspring of seed ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张虎许建民潘静霞宋微
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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