The invention discloses a mite-removing cleaning wet tissue and a preparation method thereof, and relates to the technical field of wet wipes. Base cloth raw materials comprise, by weight, 10-15 parts of polylactic acid fibers, 20-35 parts of cotton fibers, 10-15 parts of bamboo pulp fibers and 10-15 parts of wood pulp fibers, immersion liquid raw materials comprise, by weight, 5-10 parts of wormwood leaves, 15-30 parts of distilled water, 20-40 parts of purified water, 10-20 parts of clove, 10-20 parts of fructus xanthii, 10-20 parts of dandelion, 10-20 parts of cortex meliae, 10-20 parts of fructus quisqualis, 10-20 parts of flos genkwa, 10-20 parts of cortex phellodendri, 10-20 parts of radix sophorae flavescentis, 10-20 parts of herba schizonepetae, 10-20 parts of liquorice, 10-30 parts of jojoba oil, 5-15 parts of amino acid and 12-18 parts of chitosan. According to the mite-removing cleaning wet tissue and the preparation method thereof, by adding the wormwood leaves, the cloves, the fructus xanthii, the dandelions, the cortex meliae, the fructus quisqualis and other medicinal materials with the mite-removing effect, the mite-removing effect of the wet tissue can be improved, chemical components are not involved, the wet tissue is processed from pure natural extracts, after being processed, the wet tissue does not contain any chemical component, the cleaning wet tissue has the mite-removing effect, and meanwhile, antibacterial effect is also achieved.