72 results about "Mouse Colon" patented technology

The invention belongs to the field of new application of medicines, and particularly relates to gastrodia elata polysaccharide and application thereof. The gastrodia elata polysaccharide component GEP-2 is separated from crude gastrodia elata polysaccharide, structural characterization detection is conducted on the GEP-2, the result shows that the gastrodia elata polysaccharide GEP-2 is prepared from, by mass, 89.08% of glucose, 2.40% of galactose, 7.12% of galacturonic acid, 0.86% of arabinose and 0.54% of rhamnose, and the animal experiment result shows that the GEP-2 relieves the symptoms of mouse colitis, relieves weight loss and shortens the colon length; the traditional Chinese medicine composition has the advantages of reducing serum TNF-alpha, IL-1beta and IL-6 proinflammatory cytokine levels, improving the intestinal barrier function, reducing inflammatory cell infiltration, regulating the intestinal flora composition structure of mice and relieving ulcerative colitis.

The invention discloses an application of 2, 3-dihydroxybenzoic acid in preparation of an antitumor drug. Researches find that 2, 3-dihydroxybenzoic acid can inhibit proliferation, migration and invasion of colorectal cancer cells in vitro and promote cell cycle arrest and cell apoptosis, and 2, 3-dihydroxybenzoic acid can inhibit growth of mouse transplanted tumors in vivo and promote cell apoptosis; the 2, 3-dihydroxybenzoic acid has a remarkable treatment effect on mouse colon cancer and can improve the immunotherapy effect; moreover, the 2, 3-dihydroxybenzoic acid small molecule substance, as an inhibitor of fatty acid synthase (FASN), plays an important role in inhibiting colorectum.

The invention discloses a novel application of bifidobacterium breve CCFM1078 in relieving atopic dermatitis, and belongs to the technical field of microorganisms. The bifidobacterium breve CCFM1078 provided by the invention can relieve atopic dermatitis, and is specifically reflected in that (1) ear swelling and back skin thickening of mice are relieved, dermatological symptoms are relieved, and mastocyte infiltration is reduced; (2) the relative expression quantity of mRNA (messenger Ribonucleic Acid) of mouse skin barrier function related genes is increased; (3) reducing the total IgE level of mouse serum; (4) reducing the levels of IL-4, IL-13 and TSLP in mouse skin tissues, improving the levels of IL-10 and IFN-gamma, recovering Th1 / Th2 immune balance, and maintaining immune homeostasis; (5) activating the expression of AHR and CYP1A1 in the skin tissue of the mouse; (6) improving IgA and sIgA secretion of mouse colon tissue; and (7) compared with a representative high-yield ILA strain, the ILA yield is higher.

The invention discloses EGCG-myricetin carrier-free nanoparticles based on hydrogen peroxide oxidation crosslinking as well as a preparation method and application of the EGCG-myricetin carrier-free nanoparticles. The preparation method comprises the following steps: mixing amino acid, EGCG and hydrogen peroxide for reaction to prepare a covalent skeleton of an EGCG-amino acid network; and subjecting myricetin and the covalent skeleton of the EGCG-amino acid network to self-assembly so as to obtain the EGCG-myricetin carrier-free nanoparticles based on hydrogen peroxide oxidation crosslinking. According to the EGCG-myricetin carrier-free nano-particles disclosed by the invention, complex molecular design and tedious preparation schemes of a current delivery system and potential harmful effects of excipients are avoided, the prepared carrier-free nano-particles have good loading effect, stability and biological safety, and meanwhile, the EGCG-myricetin carrier-free nano-particles can obviously improve mouse colitis symptoms induced by DSS; technical support is provided for developing carrier-free nano-particles and relieving inflammatory bowel diseases, and the carrier-free nano-particles have huge clinical development prospects.

The application discloses a hybridoma cell strain 6F92E10, a monoclonal antibody thereof and application thereof, and belongs to the technical field of biotechnology.The application provides a hybridoma cell strain secreting an anti-mucinophilic Akkermansia monoclonal antibody.The antibody secreted by the hybridoma cell strain only combines with AKKermansia and does not cross-react with other common intestinal bacteria.In addition, the application solves how to detect the adhesion and colonization of mucinophilic Akkermansia in the colon of a mouse by using the prepared monoclonal antibody through an indirect immunofluorescence localization experiment, and expands the application range of the antibody.

The invention discloses a spleen-targeted nano platform construction method suitable for tumor vaccination, and particularly relates to the field of vaccines.The spleen-targeted nano platform construction method comprises the steps that S1, CL15H6-DOPS LNPs, spherical polymer vesicles and erythrocyte membrane coated nano-particles are selected as spleen-targeted nano-carriers; s2, integrating an immunologic adjuvant; mn < 2 + > doped LNPs, ultrasonic response lipidosome and a CD3 antibody are selected to be coupled to serve as an integration material; s3, delivering in vivo; animal models are selected for in-vivo delivery and curative effect verification, and B16F10 melanoma mice, MC38 colon cancer mice and non-human primates are selected as the animal models respectively. By optimizing the chain length and the surface topological structure (such as spherical polymer vesicles) of the liposome, the spleen enrichment rate is remarkably increased, and the red marrow myeloid cell uptake efficiency is enhanced. By combining with common delivery of a manganese adjuvant, an STING channel is activated, secretion of type I interferon is promoted, and the proportion of antigen-specific CD8 + T cells and the tumor inhibition rate are increased.

The invention discloses a composition for synergistically regulating intestinal flora and application thereof. The composition comprises the following components in parts by weight: 10-80 parts of a cordyceps militaris extract, 2-40 parts of quercetin, 5-50 parts of prebiotics and 5-80 parts of nucleotide. The compositions provided by the invention supplement each other, have a synergistic effect, are compounded according to a scientific proportion, can improve the diversity of intestinal microorganisms of mice and the abundance of species, and can significantly improve intestinal inflammation symptoms in a mouse colitis model.

The invention discloses preparation of a methoxyl derivative and application of the methoxyl derivative in pharmacy. The methoxy derivative as shown in the formula I has good anti-tumor activity, and the anti-tumor effect is effectively achieved through the growth inhibition and killing effect on tumor cells. The methoxyl derivative not only can inhibit the growth of CT26 transplanted tumors, but also can promote the activation and infiltration of lymphocytes in an in-vivo tumor microenvironment. Meanwhile, the compound also has a remarkable inhibiting effect on the growth of mouse colon cancer CT26 cell strains in vitro.

The invention discloses a manganese-based nano-enzyme composite material as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) preparing Mn3O4-PVP from Mn3O4 NPs and PVP10, and then loading the Mn3O4-PVP into a ZIF-8 structure in the process that Zn < 2 + > and 2-MI react to form ZIF-8, so as to obtain Mn3O4-coated ZIF-8 NPs; (2) covering the surface of the Mn3O4 ZIF-8 with HA through ion coordination, and synthesizing MZH; and (3) carrying out clathration on MZH through ES100, and synthesizing MZH coated ES100. By inhibiting a TLR4 / NF-kappa B pathway, activating an Nfr2 / Keap1 pathway and increasing expression of intestinal tight junction protein, mouse colitis induced by DSS is resisted, and symptoms of the mouse colitis are relieved.

The invention belongs to the technical field of microorganisms, and discloses clostridium butyricum ML5358 and application thereof.The clostridium butyricum ML5358 provided by the invention has the preservation number of CGMCC No.37000. The strain can effectively relieve mouse colitis induced by DSS and remarkably reduce expression of colon tissue inflammatory factors Il-6 and Il-1beta, and the preservation number of the clostridium butyricum ML5358 is CGMCC No.37000. The clostridium butyricum ML5358 is CGMCC No.37000. The clostridium butyricum ML5358 is CGMCC No.37000. In addition, skeletal muscle lipid metabolism can be regulated and controlled through the intestine-muscle axis, expression of fat synthesis and uptake related genes Srebf1, Acc1 and Fabp4 is specifically up-regulated, the content of intramuscular triglyceride is remarkably increased, and intramuscular fat deposition is promoted. According to the clostridium butyricum ML5358 and the application thereof, the functional limitation of traditional clostridium butyricum is broken through, and a new strain resource is provided for developing a novel microbial preparation or a feed additive capable of improving intestinal health and improving livestock and poultry meat quality.

The invention provides an application of a heat shock protein 90 inhibitor in preparation of a medicine for treating intestinal injury and complicated intestinal inflammation thereof. The HSP90 inhibitor is suitable for treating colon tissue injury caused by a medicine for inducing Gasdermin E (GSDME) mediated pyroptosis. The HSP90 inhibitor specifically inhibits the expression of GSDME protein in normal intestinal epithelial cells and reduces the sensitivity of the normal intestinal epithelial cells to drug-induced GSDME-mediated pyroptosis, so that the damage to colon tissues is relieved, and the induced inflammatory response of the colon tissues is effectively inhibited. TAS-116 and BIIB021 are used as representative drugs of the HSP90 inhibitor, 4 mg / kg of TAS-116 and BIIB021 are used for a mouse colon injury model constructed by 5-FU and CDDP, and the result shows that the HSP90 inhibitor can obviously improve weight loss and colon tissue injury of mice caused by the drugs, and has a good application prospect. Therefore, the HSP90 inhibitor has remarkable curative effect and application value in the aspect of relieving or preventing and treating intestinal injury caused by drugs.

The invention provides a medicine for preventing or treating enteritis. Hamp is adopted; and E, dyeing, determination of IL-6 factors in serum of each group of animals and the like are used for researching the influence of the vocyclosporine on intestinal tissues, mucosal barriers and immune systems of DSS or TNBS-induced mouse colitis models. Experimental basis is provided for clinical application of the vocyclosporine in preparation of the colitis treatment medicine, and the vocyclosporine has good application prospects and huge potential value and social significance.

A polypeptide specifically blocking the binding of ACVR1C to GREM1 is provided. The small-molecule polypeptide interferes with or blocks the binding of GREM1 to ACVR1C in vitro and in vivo. The liver metastasis of colon cancer in mice in a treatment group is significantly lowered, which indicates that the small-molecular polypeptide achieves the blocking of the liver metastasis of colon cancer.

The invention discloses an antigen expression vector, a combination of the antigen expression vector and an antibody and application of the combination in cancer treatment. The antigen expression vector comprises one or more DMP-antigen coding gene units; the DMP-antigen coding gene is composed of two functional elements DMP and an antigen coding gene, the DMP is an NF-kappa B specific promoter, and the antigen coding gene is an antigen molecule coding sequence. The antigen expression vector prepared by the invention can selectively express antigen molecules in cancer cells, an in-vivo delivery vector taking adeno-associated virus as the antigen expression vector, and a combination of an antigen CD20 expressed by the antigen expression vector and an anti-CD20 antibody rituximab. The compound has a good treatment effect on mouse colon cancer on mice and human colon cancer on humanized mice, and has good tumor targeting property and safety in the treatment of the cancer mice. The invention is expected to provide a new technology and a new reagent for the treatment of various cancer diseases.

The present invention belongs to the field of microorganisms, and specifically relates to the use of Lactobacillus rhamnosus AFY01 and a product thereof in inflammatory colon cancer. Provided is a strain of Lactobacillus rhamnosus AFY01 with the deposit number of CGMCC No. 27362. The AFY01 can effectively alleviate weight loss in mice with colon cancer and colon shortening caused by intestinal inflammation and edema, reduce the colon coefficient of mice, reduce the visceral index and reduce the occurrence of intestinal tumors in mice, alleviate intestinal inflammation, and promote intestinal tumor cell apoptosis to slow down the development of colon cancer. By means of the present invention, the strain resources are expanded, thus providing technical support for the prevention and treatment of intestinal cancers.

This invention discloses the application of chestnut total burr ethanol extract in the preparation of anti-colitis drugs. In this invention, the chestnut total burr ethanol extract with anti-colitis properties is obtained by extracting chestnut total burrs with 70% ethanol. This chestnut total burr ethanol extract inhibits the production of LPS-induced pro-inflammatory mediators (NO) and pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β) without cytotoxicity; it has a significant inhibitory effect on dextran sulfate sodium-induced colitis in mice, including slowing down weight loss, reducing mouse DAI scores, alleviating colonic tissue damage, downregulating the levels of IL-6, IL-1β, TNF-α, and MDA in mouse colonic tissue and serum, and enhancing the activities of CAT and SOD in mouse colonic tissue and serum. Therefore, the chestnut total burr ethanol extract has significant anti-inflammatory effects both in vivo and in vitro, and can be used to treat colitis.

The present application relates to the technical field of microorganism, and particularly relates to a luohanguo flavor dairy product and a preparation method and application thereof, the present application adopts buffalo milk, luohanguo powder, paracasei and rhamnosus to prepare the flavor dairy product, it is found during the preparation process research that the luohanguo powder, paracasei and rhamnosus have good synergies, the luohanguo powder fermented dairy product prepared by the combination has good antioxidant function, through animal experiment, the product can effectively relieve the inflammation of DSS-induced mouse colon, and the effect of the luohanguo flavor fermented milk is close to that of positive drug, so the product is a good diet therapy product.

The application discloses a method for relieving the adverse effects of chronic restraint stress on animals by adding sodium isovalerate into feed, which comprises taking C57BL / 6J mice as research objects, studying the effects of sodium isovalerate on growth performance, depression behavior and intestinal barrier and inflammation indexes of the mice with chronic restraint stress, recording daily weight gain and feed intake, and performing tail suspension test and Elisa test and other test methods, and proving that sodium isovalerate can relieve the decrease of mouse weight growth rate, the increase of feed to weight ratio and the decrease of organ index caused by CRS; through fluorescence quantitative PCR and Western Blot and other methods, it is proved that sodium isovalerate can relieve the intestinal injury of the CRS mice, reduce the expression of colon pro-inflammatory factors and improve the expression of tight junction proteins, and can also reduce the phosphorylation level of NF-kappa B in the colon of the CRS mice. The application explores the relieving effect of sodium isovalerate on chronic restraint stress of animals, so as to provide some theoretical basis for relieving stress in actual production and the application of sodium isovalerate in feed additives.

The invention provides a construction method of engineered lactic acid bacteria for constitutive expression of SodA protein and application of the engineered lactic acid bacteria in treatment of IBD. The invention provides a construction method of engineered lactic acid bacteria (KI (at) lSodA) for relieving IBD (Infectious Bronchitis Disease) constitutive expression SodA protein. A verification experiment result shows that the KI (at) lSodA highly expresses the SodA protein, and the oxidative stress management capability of the strain is improved; meanwhile, the KI (at) lSodA also has long-term use safety, the treatment of the KI (at) lSodA remarkably relieves the colitis symptoms and in-vivo inflammation conditions of mice in an acute stage, a recovery stage and a chronic state, and the KI (at) lSodA shows a strong intestinal microbial diversity recovery capability in all three experimental models, and particularly shows a remarkable repair effect in a recovery stage model. Therefore, the invention provides a new basis for IBD treatment.

The invention discloses application of a waxberry seed alcohol extract in preparation of a medicine for treating colitis. The waxberry seed alcohol extract inhibits the generation of LPS-induced proinflammatory mediators (NO) and proinflammatory cytokines (TNF-alpha, IL-6 and IL-1beta) under the condition of no cytotoxicity; the waxberry seed alcohol extract inhibits the expression of the protein of LPS-induced pro-inflammatory mediator enzymes (iNOS and COX-2); the waxberry seed alcohol extract not only inhibits nuclear transfer of NF-kappa B by reducing phosphorylation and degradation of I kappa B alpha, but also inhibits phosphorylation of MPAKs (ERK, p38 and JNK); animal experiments prove that the waxberry seed alcohol extract shortens mouse colon, reduces mouse DAI score, alleviates mouse colon tissue damage, reduces the content of IL-6, IL-1beta, TNF-alpha and MDA in mouse colon tissue and serum, and enhances the activity of CAT and SOD in mouse colon tissue and serum. Therefore, the waxberry seed alcohol extract has a remarkable anti-inflammatory effect in vivo and in vitro, and can be used for treating colitis.

The application belongs to the technical field of microorganisms, and discloses Lactobacillus fermentum (L.f) LFSJ001 and application thereof in preparation of products for preventing or treating ulcerative colitis. The Lactobacillus fermentum LFSJ001 is independently isolated and identified. It is found through the use of DSS to construct a mouse colitis model that the Lactobacillus fermentum LFSJ001 combined with 5-Aminosalicylic Acid (5-ASA) can enhance the improvement of 5-ASA on the symptoms of colitis, including multiple indexes such as disease activity index, colon injury, and spleen index. It can be seen that the Lactobacillus fermentum LFSJ001 can be used as a combined drug of 5-ASA, enhance the efficacy of 5-ASA, inhibit the occurrence and development of ulcerative colitis, and improve the intestinal barrier. The application provides a new diagnosis and treatment direction and strategy for the prevention and treatment of ulcerative colitis.

This invention discloses a composition comprising probiotics and a fusion polypeptide and its application in the treatment of inflammatory bowel diseases. The composition consists of *Lactobacillus plantarum* CCFM8661 and a functional fusion polypeptide, wherein the amino acid sequence of the fusion polypeptide is shown in SEQ ID NO.1. This fusion polypeptide achieves a triple synergistic effect of "antibacterial inhibition-repair-immunomodulation" by enhancing the intestinal colonization ability of probiotics, directly inhibiting the growth of pathogenic bacteria, and neutralizing toxins. Experiments show that the composition significantly reduces the disease activity index, restores colon length, and inhibits serum inflammatory factors IL-6 and TNF-α in a DSS-induced mouse colitis model. Furthermore, the lyophilized microcapsule formulation exhibits a survival rate of >90% in simulated gastric juice and possesses colon-targeted release characteristics. This invention provides a highly efficient and stable new treatment strategy for inflammatory bowel diseases.

The present invention provides a kind of Chinese medicine preparation for treating ulcerative colitis and its preparation method, relate to the field of Chinese medicine pharmaceutical technology. The Chinese medicine preparation of the present invention is made of 10-30 parts of red cauliflower, 10-30 parts of mulberry leaves, 5-20 parts of sophora japonica flowers, 5-20 parts of lotus seeds, 1-15 parts of chalk, 1-15 parts of Chinese toon root bark, 5-20 parts of lily, 5-20 parts of pseudoginseng, 1-15 parts of white labiatae, 5-20 parts of lindera striata, and 5-20 parts of fructus aurantii, with the effects of clearing away heat and detoxifying, astringing the intestines and stopping diarrhea, regulating the spleen and stomach qi, and regulating qi and yin. The Chinese medicine preparation of the present invention can significantly improve the trend and clinical symptoms of weight loss in UC mice, as well as the situation of shortened colon in UC mice, can repair and improve the integrity of colon tissue structure, significantly inhibit the secretion of inflammatory factors IL-6, IL-1β and TNF-α, improve the inflammatory situation of colon tissue, and has anti-inflammatory and anti-inflammatory effects. The Chinese medicine preparation of the present invention can significantly improve the cure rate and total effective rate of ulcerative colitis, improve and relieve the patient's symptoms such as abdominal pain, diarrhea, bloody stools and tenesmus, and greatly reduce the patient's pain. The Chinese medicine preparation of the present invention can be promoted and applied as a medicine for treating ulcerative colitis.

The invention relates to the technical field of biological medicine, and discloses dual-network hydrogel for treating inflammatory bowel disease as well as a preparation method and application of the dual-network hydrogel. The preparation method of the double-network hydrogel comprises the following steps: dissolving glycyrrhizic acid into deionized water, and then adding beta-lactoglobulin to obtain a solution A; dissolving zinc chloride and 2, 3, 4-trihydroxybenzoic acid in deionized water to obtain a solution B; mixing the solution A and the solution B in proportion, and stirring for reaction to obtain the double-network hydrogel. A main framework of the double-network hydrogel is composed of a first-stage network formed by glycyrrhizic acid and zinc ions through self-crosslinking and a second-stage network formed by beta-lactoglobulin and 2, 3, 4-trihydroxybenzoic acid through Schiff base and hydrogen bond crosslinking. The invention provides the dual-crosslinked hydrogel with the skeleton being a therapeutic effective component and good plasticity and adhesion, and the dual-crosslinked hydrogel has the advantages of resistance to external severe environment and strong anti-inflammatory ability, and can effectively relieve mouse colitis.

Lacticaseibacillus rhamnosus AFY01 and an application of a product prepared from Lacticaseibacillus rhamnosus AFY01 in inflammatory colon cancer are provided, which relates the field of microbes. A preservation number of the Lacticaseibacillus rhamnosus AFY01 is CGMCC No. 27362. The Lacticaseibacillus rhamnosus AFY01 can effectively alleviate weight loss, intestinal inflammation and edema induced colon shortening in mice with colon cancer, reduce colon coefficient, visceral index and incidence of intestinal tumors in mice, improve intestinal inflammation, promote apoptosis of intestinal tumor cells to slow down the development of colon cancer. The Lacticaseibacillus rhamnosus AFY01 expands bacterial resources to provide technical support for the prevention and treatment of colorectal cancer.

The invention discloses siRNA (small interfering Ribonucleic Acid) for inhibiting the expression of a CREPT gene. The nucleotide sequence of a positive-sense strand of the siRNA is shown as any one of SEQ ID No. 1 to SEQ ID No. 3. The siRNA can be used for inhibiting CREPT (Cathode Reactive Part of siRNA can inhibit the growth of mouse colon cancer cells and the growth of NIH3T3 fibroblast cells. And the drug can be delivered through the liposome of the delivery system si-CREPT, so that the mouse liver cancer can be treated, and the tumor load of the mouse liver cancer can be remarkably reduced.

The application discloses a phosphine-containing oxypentacyclic compound and a preparation method thereof, relates to the technical field of organic synthesis, and discloses a high-efficiency radical hydrophosphonylation reaction of 1,6-alkenyl alkyne and a secondary phosphine oxide compound through visible light oxidation-reduction catalysis. Under simple operation and mild conditions without alkali, various pentacyclic compounds containing a secondary phosphine oxide group are obtained. The preparation method has good regioselectivity, is easy to expand, and the prepared phosphine-containing oxypentacyclic compound has a good inhibitory effect on mouse colon cancer cells MC38.

The invention provides application of barley pest larva defatted protein powder in preparation of drugs for treating inflammatory bowel diseases. The invention belongs to the technical field of medicine, and by establishing a dextran sodium sulfate induced mouse IBD model and lavaging barley pest larva defatted protein powder, it is found that the traditional Chinese medicine composition can relieve IBD mouse colitis symptoms, restore colon length, relieve mucosal lesion, retain goblet cell and crypt structures, reduce inflammatory cell infiltration and down-regulate inflammatory cytokine expression; the compound is expected to play an important role in preparing medicines for treating inflammatory enteritis.

Through virtual screening based on molecular docking, molecular dynamics simulation, free energy calculation and experimental verification, it is found for the first time that benfotiamine can efficiently antagonize P2Y14R, and the IC50 value of benfotiamine reaches 0.31 nM. Experimental results show that benfotiamine can significantly relieve inflammatory reactions of a DSS-induced mouse colitis model and an MSU-induced gouty arthritis model, and the action mechanism of benfotiamine is closely related to inhibition of activation of NLRP3 inflammasomes. Benfotiamine is used as a vitamin B1 derivative clinically applied for a long time and has good safety and tolerance. The invention provides a brand new pharmacological action target and indication direction, realizes new use of old medicines, is beneficial to shortening the research and development period of medicines and reducing the development cost, and has important application prospects and economic and social values in the field of treatment of inflammatory diseases.

The application discloses a kind of composite microecological preparation and preparation method and application, belong to microbial technical field.The composite microecological preparation is core-shell structure, the core-shell structure is divided into inside and shell layer, inside includes Bifidobacterium adolescentis, shell layer includes metal polyphenol network and ficoll polysaccharide;The metal polyphenol network includes the complex coating formed by pterostilbene and calcium ion.The core is BA, the shell is made of FCPS, PTE, Ca 2+ Network composition, can protect Bifidobacterium adolescentis to reach intestine successfully and colonize, directly inhibit inflammatory response, and relieve CDI-induced mouse colitis and oxidative stress damage by regulating intestinal flora, indole and the generation of indole derivatives, solve the pain point that traditional microecological preparation probiotic survival rate is low, poor targeting, limited effect.