32 results about "Wood tissue" patented technology

The invention discloses a rooting basal medium (BPM) and a cultivation method thereof, characterized in that the components of the MS basal medium are improved, rooting inducers (NAA, activated carbon) are added, and culture conditions are adjusted. , the rooting rate in the bottle can reach 100% in 14 days, so that the transplanting survival rate can reach more than 90%, which is 12%~34% higher than the current report. It only takes 45 days from inoculation to transplanting to survive, which not only improves the rooting efficiency of the tissue-cultured seedlings of Lime plum, but also has the characteristics of neat and robust seedlings, saving costs and high efficiency, and shortening the cultivation cycle of tissue-cultured seedlings of Lime plum. Chemical production provides the means to rapidly produce high-quality seedlings.

The invention relates to the technical field of wood tissue culture seedling raising in forest science, in particular to a Tamarix chinensis tissue culture and rapid propagation method. The method includes: acquisition of aseptic seedlings, with an involved medium containing 6-BA, IBA, GA, sucrose and agar; subculture: inoculating the aseptic seedlings into a subculture medium to conduct culture so as to obtain clustered shoots, with the medium containing ZT, IBA, sucrose and agar; rooting culture: inoculating the clustered shoots into a rooting medium containing IBA, NAA, GA, sucrose and agar to obtain rooted tissue cultured seedlings; and acclimatization and transplantation. The propagation method has high propagation coefficient and short propagation cycle, the obtained seedlings are strong and have a lot of root systems, and the survival rate of transplantation is over 96%. The method provided by the invention omits the strong seedling culture step in conventional methods, improves propagation efficiency, makes the Tamarix chinensis tissue culture and rapid propagation technology move towards production from laboratories, realizes large-scale production of Tamarix chinensis tissue culture seedlings, and provides technical support for promotion of Tamarix chinensis rapid propagation.

The invention discloses a primary fir wood culture bud induction method. The method comprises the following steps: inoculating an explant as a coppice shoot of a fir wood base part and subjected to sterile explant treatment to a bud induction culture medium subjected to high-quality primary culture, culturing under the conditions of proper temperature, humidity and illumination intensity, and inducing bud germination. The conventional fir wood induction technology is improved, the problems that the explant is subjected to browning and vitrifaction in the fir wood bud induction process and the like are solved, the fir wood bud induction rate, the budding index and the germination and growth conditions are improved, and large-number high-quality buds are obtained, so that the multiplication culture requirement is met, the establishment of a rapid fir wood tissue culture reproductive system is promoted, and the method has high economic benefits and social benefits.

The invention discloses a method for accurately extracting DNA from wood targeted cells; through removal of exogenous pollution on a wood surface, in-situ detection of wood DNA, radial section of woodtissues, precise separation of the wood targeted cells enriched in DNA under an inverted microscope, cracking of wood cell walls, removal of a wood extract, DNA precipitation and adsorption, dissolution, purification, PCR amplification and other steps, DNA is extracted accurately from the wood tissues. The method aims at the wood targeted cells to carry out DNA accurate extraction, the quantity and purity of DNA extracted from the wood tissues are improved, and the interference of other types of cells of wood on DNA extraction is reduced. The method can be directly applied to study of molecular biology and provide a new technical support for identification of wood species.

The invention provides a tissue culture method of acrocarpus fraxinifolius. The tissue culture method comprises the following steps: step 1. selecting an explant; step 2. treating the explant; step 3. inoculating; step 4. producing an induction culture medium; step 5. producing a subculture medium; and step 6. culturing a strong seedling, wherein the production formula of the induction culture medium comprises 1mg / L of MS+6BA, 0.2mg / L of NAA, 30g of white sugar and 6.5g of agar, and the production and selection formula of the subculture medium comprises 0.6mg / L of MS+6BA, 0.2mg / L of NAA, 30g of white sugar and 6.5g of agar. According to the vegetative rapid reproduction method of acrocarpus fraxinifolius, three different culture mediums are used for culturing acrocarpus fraxinifolius, and plants with ordered appearance can be reproduced in a large scale in relatively short time, and the variation of the regenerated plants is low; a regeneration system is very efficient; and the average cost of a single regenerated seedling is extremely low, the large-batch efficient and rapid reproduction of acrocarpus fraxinifolius is facilitated, so that the tissue culture method is used for industrial production.

The invention provides a tissue culture and rapid propagation method of euryodendron excelsum. The method comprises the following steps: selecting and disinfecting explants, inducing clustered buds, proliferating clustered buds, rooting and culturing, culturing strong seedlings and culturing a seedling bed. Compared with the conventional seedling-raising method of euryodendron excelsum, the method does not adopt euryodendron excels seeds; the new sprout seedlings of the euryodendron excelsum are adopted; few plant raw materials are needed; the euryodendron excelsum is subjected to short-time and mass propagation; the heritable variation of the variety is low; the young seedlings is high in quality; the survival rate of the young seedlings reaches up to 90%; the survival rate of transplanted seedlings reaches up to 80%; after transplanting, the seedlings are excellent in growth vigor; the phenomenon of small seedling is avoided; the problems that the euryodendron excelsum wild resources are increasingly reduced and natural reproduction of the population is difficult can be effectively solved; the technical support is provided for effective protection of euryodendron excelsum serving as a rare and threatened plant.

The invention provides a rice paper plant tissue culture rapid breeding method, and belongs to the technical field of agriculture planting. The method includes the steps of conducting surface disinfection, callus inducement, bud differentiation culture and multiplication, rooting induction, plant exercising and transplantation with rice paper plant young leaves or young stems or young buds as explant. The tissue culture rapid breeding technical system for rice paper plants is established for the first time, the operation method is easy, convenient and feasible, the breeding coefficient is high, 20 times to 25 times of multiplication can be achieved every 60 days, the tissue culture seedling rooting rate reaches 98% or above, the plant exercising and transplanting survival rate reaches 92% or above, and the large-scale production can be conducted.

The invention discloses a tissue culture disinfection and sterilization formula of ormosia henryi prain and a tissue culture method of the ormosia henryi prain. Seed seedling stems are disinfected by 70% ethanol for 30 seconds, sterilized by 0.1% mercury bichloride for 8 minutes, disinfected by the 70% ethanol for 30 seconds and treated by 0.5ml / L of a plant tissue culture antibacterial agent to achieve the best disinfection effect; the optimal collection time of seedling explants is April; the stems are immersed by the 70% ethanol for 30 seconds, immersed by a mixed solution (containing 50mg / L rifampicin and 50mg / L chloramphenicol) for 30 minutes and immersed by 0.1% mercury bichloride for 8 minutes to achieve the best disinfection effect; and leaves are immersed by the 70% ethanol for 30 seconds, immersed by the mixed solution (containing 50mg / L rifampicin and 50mg / L chloramphenicol) for 30 minutes and immersed by 0.1% mercury bichloride for 6 minutes to achieve the best disinfection effect. Leaves of grown plants are relatively suitable for collecting in April, and are immersed by the 70% ethanol for 30 seconds, immersed by the mixed solution (containing 50mg / L rifampicin and 50mg / L chloramphenicol) for 30 minutes and immersed by 0.1% mercury bichloride for 12 minutes. According to the tissue culture disinfection and sterilization formula and the tissue culture method, seed seedlings, seedlings and grown plant tissues of ormosia henryi prain explants are disinfected by different disinfection methods, and the relatively good survival rate can be obtained; and sources of ormosia henryi prain tissue culture multiplication materials are greatly expanded, and the tissue culture effect of the ormosia henryi prain can be greatly improved by controlling disinfection time.

The invention discloses a sequoia sempervirens steam section induction plant regeneration tissue culture rapid propagation method, and belongs to the technical field of forest wood tissue culture breeding. The method belongs to a propagation method characterized in that the screened sequoia sempervirens high-quality plants are used as female parents; the annual stems with buds are selected to be used as explants for inducing normal bud germination; then, aseptic seedlings are used for induction to generate adventitious buds; then, the adventitious buds are used for inducing regeneration plantsto root for seedling domestication and transplanting potting. The sequoia sempervirens fast breeding technology is used for mass production; the method is simple and convenient; the regeneration period is short; the propagation coefficient is high; the transplanting survival rate is high; the seedling germination is regular and consistent; the maintenance and management are convenient; the laborand land resources are saved; the production cost is low; the season limitation is avoided; the regeneration plants are continuously obtained; the method can be used for seedling industrialized production.

The invention relates to a method for planting patterns of solid wood or solid wood composite flooring. It comprises the following steps: a. Sanding the wooden floor plain board or light board blank; b. Coating the surface of the wooden floor plain board or light board blank; c. Using a material with a certain shape on the wooden floor plain board Or make texture traces on the surface of the bare board blank material; d. Dry the wooden floor plain board or light board blank material after the texture traces are made and the paint is applied. After adopting the above method, wood tissue, planted texture and paint can be integrated into a unified whole, and when the wood tissue is further hardened and shaped, texture with natural color and luster can be obtained.

The invention discloses a composite material of titanium dioxide and charcoal and a preparation method thereof, and belongs to the field of semiconductor photocatalysts; the microstructure of the carbon in the composite material of titanium dioxide and charcoal maintains the structure of wood tissue, and the titanium dioxide is uniformly dispersed on the surface of the charcoal. Titanium dioxide has both anatase and rutile phases; the method first pretreats the woody biological template; then the woody biological template is impregnated in the precursor solution, and the TiO 2 Particles are deposited in the micropores of the wooden formwork to obtain a woody biological template with titanium dioxide deposited, and finally roasted in a muffle furnace to obtain a titanium dioxide / charcoal composite photocatalyst; the composite material of titanium dioxide and charcoal prepared by the present invention can be used to remove The adsorption removal rate of environmental endocrine disruptors and other organic pollutants in water is 32.3-86.5%, the photocatalytic removal rate is 11-20%, and the total adsorption / photocatalytic synergistic removal rate can reach 50.3-97.5%. Good application prospects.

The invention relates to a method for making an anti-abrasion and fire-retardant panel by quick growing wood. The method comprises the following processes: rotatably cutting a single board, preparing fire-retardant soaking glue, soaking the single board into the fire-retardant soaking glue, laying and densifying the panel and so on. The fire-retardant soaking glue is preparing by mixing urea-modified melamine glue, an intensifier and a flame retardant; the single board is obtained by rotatably cutting the quick growing wood; a vacuum pressurizing and impregnating process is adopted to make the soaking glue infilter wood tissues, so as to improve the performances of fire retardance, smoke abatement and agglutination; then three pieces of single boards are stacked and laid through crisscrossing textures of the single boards; and the thickness of the panel is 4+ / - 0.5 mm, and the density reaches 1.0 to 1.2g / cm3 after the single boards are hotly pressed and densified. The panel has the advantages of fire retardance, smoke abatement, stable abrasion resistance, strong mildew resistance and strong weather resistance.

The invention provides a method for tissue culture and fast propagation of Chongyang wood, which uses Chongyang wood with axillary bud stem segments or Chongyang wood leaves as explants for tissue culture propagation. When the explants are Chongyang wood with axillary bud stem segments, the cultivation method is: sterilize the bud stem segments, then place the bud stem segments in the induction medium to directly obtain clump seedlings, and then cut the clump seedlings into individual seedlings and inoculate them one by one. Rooting culture is carried out on the rooting medium, and finally the seedlings after rooting and culture are refined and transplanted in sequence, so as to realize the rapid propagation of Chongyang tree. When the sterile leaves of Chongyang wood are used as explants, the culture method is as follows: sterilize Chongyang wood seeds, and cultivate the sterile leaves, then use the sterile leaves as the explants, and sequentially induce callus. , Callus differentiation, rooting culture process, the establishment of Chongyang wood tissue culture rapid propagation system. The invention has the advantages of simple operation, low cultivation cost, high reproduction efficiency, little influence by natural environment, and good market and industrial application prospect.

The invention discloses a method for culturing bloodwood tissue. The present invention uses the hypocotyls of the blood tree seeds as explants to successfully carry out tissue culture and rapid propagation of the high-quality seedlings of the blood tree of the excellent strain, and the produced seedlings of the blood tree have high genetic stability, Rapid emergence, strong seedlings, good seedling quality, strong resistance, good growth and other characteristics; it has a good market prospect.