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32 results about "Wood tissue" patented technology

Wood is a porous and fibrous structural tissue found in the stems and roots of trees and other woody plants. It is an organic material, a natural composite of cellulose fibers that are strong in tension and embedded in a matrix of lignin that resists compression.

Flora and method for producing Chinese eaglewood wood on Aquilaria senensis (Lour.) Gilg by bottle interpolation method

The invention belongs to the field of manually planted Aquilaria senensis (Lour.) Gilg and relates to a microbial flora preparation for producing Chinese eaglewood wood and a matched bottle interpolation method. Eight endophytic strains of the Chinese eaglewood wood such as colored Botryodiplodia theobromae Pat are extracted and separated from natural Chinese eaglewood wood tissues, and are respectively disinfected and purified to obtain liquid flora preparations with Chinese eaglewood wood production capacity, and the obtained liquid flora preparations jointly act on the Aquilaria senensis (Lour.) Gilg. Meanwhile, the conventional matched infusion method is improvement on the bottle interpolation method; and by the ingenious bottle interpolation method, the disadvantages that a complete set of infusion apparatus is depended and the dripping speed is required to be constantly observed and adjusted in the conventional method are overcome. By the method, a microbial inoculum can be manually immersed into calluses of healthy Aquilaria senensis (Lour.) Gilg in batches and penetrate and infect protoxylem to promote a micro-circulation system in the protoxylem to secrete resin and produce the Chinese eaglewood wood in an abnormal metabolism mechanism.
Owner:唐显

Titanium-dioxide charcoal composite material and preparation method thereof

The invention discloses a titanium-dioxide charcoal composite material and a preparation method thereof, and belongs to the field of semiconductor photocatalysts. According to the titanium-dioxide charcoal composite material, the microstructure of charcoal keeps the structure of wood tissue, titanium dioxide is uniformly dispersed on charcoal surface, and titanium dioxide has anatase phase and rutile phase at the same time. The method comprises: firstly performing pretreatment on a wood biological template; dipping the wood biological template in a precursor solution, using a sol-gel method or a hydro-thermal method to depositing TiO2 particles in micropores of the wood template, so as to obtain the wood biological template of titanium dioxide; and finally sintering in a muffle furnace to obtain the titanium-dioxide charcoal composite photocatalyst. The prepared titanium-dioxide charcoal composite material is applicable to remove environmental endocrine disruptors in water and other organic pollutants in water, has the adsorption removal rate of 32.3-86.5%, the photocatalysis removal rate of 11-20% and the total adsorption / photocatalysis synergic removal rate of 50.3-97.5%, and has relatively good application prospect.
Owner:KUNMING UNIV OF SCI & TECH

Method for producing wear-resistant flame-retardant panel from fast growing wood

The invention relates to a method for making an anti-abrasion and fire-retardant panel by quick growing wood. The method comprises the following processes: rotatably cutting a single board, preparing fire-retardant soaking glue, soaking the single board into the fire-retardant soaking glue, laying and densifying the panel and so on. The fire-retardant soaking glue is preparing by mixing urea-modified melamine glue, an intensifier and a flame retardant; the single board is obtained by rotatably cutting the quick growing wood; a vacuum pressurizing and impregnating process is adopted to make the soaking glue infilter wood tissues, so as to improve the performances of fire retardance, smoke abatement and agglutination; then three pieces of single boards are stacked and laid through crisscrossing textures of the single boards; and the thickness of the panel is 4+ / - 0.5 mm, and the density reaches 1.0 to 1.2g / cm3 after the single boards are hotly pressed and densified. The panel has the advantages of fire retardance, smoke abatement, stable abrasion resistance, strong mildew resistance and strong weather resistance.
Owner:ZHEJIANG FORESTRY UNIVERSITY

Primary fir wood culture bud induction method

The invention discloses a primary fir wood culture bud induction method. The method comprises the following steps: inoculating an explant as a coppice shoot of a fir wood base part and subjected to sterile explant treatment to a bud induction culture medium subjected to high-quality primary culture, culturing under the conditions of proper temperature, humidity and illumination intensity, and inducing bud germination. The conventional fir wood induction technology is improved, the problems that the explant is subjected to browning and vitrifaction in the fir wood bud induction process and the like are solved, the fir wood bud induction rate, the budding index and the germination and growth conditions are improved, and large-number high-quality buds are obtained, so that the multiplication culture requirement is met, the establishment of a rapid fir wood tissue culture reproductive system is promoted, and the method has high economic benefits and social benefits.
Owner:GUANGXI FORESTRY RES INST

Tamarix chinensis tissue culture and rapid propagation method

The invention relates to the technical field of wood tissue culture seedling raising in forest science, in particular to a Tamarix chinensis tissue culture and rapid propagation method. The method includes: acquisition of aseptic seedlings, with an involved medium containing 6-BA, IBA, GA, sucrose and agar; subculture: inoculating the aseptic seedlings into a subculture medium to conduct culture so as to obtain clustered shoots, with the medium containing ZT, IBA, sucrose and agar; rooting culture: inoculating the clustered shoots into a rooting medium containing IBA, NAA, GA, sucrose and agar to obtain rooted tissue cultured seedlings; and acclimatization and transplantation. The propagation method has high propagation coefficient and short propagation cycle, the obtained seedlings are strong and have a lot of root systems, and the survival rate of transplantation is over 96%. The method provided by the invention omits the strong seedling culture step in conventional methods, improves propagation efficiency, makes the Tamarix chinensis tissue culture and rapid propagation technology move towards production from laboratories, realizes large-scale production of Tamarix chinensis tissue culture seedlings, and provides technical support for promotion of Tamarix chinensis rapid propagation.
Owner:QINGDAO ZHENGJIE INDAL

Outdoor bamboo wood natural mould-proof corrosion-proof agent and preparation and treatment methods thereof

The invention relates to the technical field of bamboo wood mould proofing and corrosion proofing, in particular to an outdoor bamboo wood natural mould-proof corrosion-proof agent and preparation andtreatment methods thereof. The bamboo wood natural mould-proof corrosion-proof agent is prepared from the following components in percentage by weight: 30-40% of industrial wastewater after stevia rebaudiana is processed to prepare stevioside, 5-10% of soapberry fruit, 20-30% of melia azedarach leaf, 10-15% of folium coptis chinensis and 10-20% of folium isatidis. According to the preparation method of the bamboo wood natural mould-proof corrosion-proof agent, conventional decoction or alcohol extraction is adopted; the method for treating bamboo wood by means of the bamboo wood natural mould-proof corrosion-proof agent comprises the steps that after the bamboo wood is placed in hot water for soaking treatment or placed in an ethyl alcohol solution for soaking treatment, the bamboo wood natural mould-proof corrosion-proof agent is used for treatment. The outdoor bamboo wood natural mould-proof corrosion-proof agent and the preparation and treatment methods thereof have the beneficialeffects that firstly, the bamboo wood is placed in the alcohol solution for soaking to make large amounts of starch, sugar, protein and other salt substances in bamboo wood tissues are extracted through alcohol; therefore, the condition that the natural organic mould-proof corrosion-proof agent penetrates through parenchyma cells and vascular bundles of the internal tissues of the bamboo wood is more easy; the mould-proof corrosion-proof agent is prepared from the natural organic extracts and do not have any toxicity or cause pollution problems to the environment.
Owner:涂世红

Method and tool for machining rod-shaped reconstructed wood unit

The invention discloses a method and a tool for machining a rod-shaped reconstructed wood unit. The method comprises the following steps of: cutting wood along the longitudinal direction of the fiber of the wood according to the set depth by using a scratching knife with more than two blades without sawdust; generating lateral separation and generating a scratching knife mark by the wood tissue with the set depth; cutting along the longitudinal direction of the wood by using the scratching knife blade in the direction vertical to the depth of the scratching knife mark; and cutting the wood toform a wood-wool rod. The middle boss of the base of a cutter is provided with dovetail grooves; one side surface of each dovetail groove is provided with a rectangular path; a plane cutter is arranged in the dovetail groove on the side, and the scratching knife is installed in the dovetail groove on the other side; the scratching knife and the plane cutter are fixed on two sides of the dovetail groove by press cakes; the scratching knife and the plane cutter are higher than the top surface of the boss; and a gap is formed between the inclined bottom surface of each press cake and the blade of the plane cutter. The wood-wool rod is easy to realize directional pavement when a wood assembly is reconstructed. The wood is cut longitudinally along the vertical grain without sawdust. The tool has the advantages of small noise, small cutting resistance, no dust pollution and high processing efficiency.
Owner:ZHEJIANG FORESTRY UNIVERSITY

Genes controlling plant cell wall formation

A Eucalyptus EST database was prepared and used for extracting genes specifically expressed in Eucalyptus reaction wood tissues using microarray analysis. As a result, genes were broadly classified into a gene cluster with a predominantly high expression, a gene cluster with a lower expression, and a gene cluster that is virtually unchanged, in Eucalyptus reaction wood as compared to ordinary trunks. It is thought that the gene cluster with predominantly high expression and the gene cluster with low expression can be used to control cell wall biosynthesis and wood fiber cell morphogenesis
Owner:OJI PAPER CO LTD

Dissoluble cleaning tissue wholly made of wood pulp

The invention discloses a dissoluble cleaning tissue wholly made of wood pulp. The dissoluble cleaning tissue is characterized in that a wood pulp tissue body is included and comprises a wood pulp fiber long diameter part and a wood pulp fiber short diameter part, the wood pulp fiber long diameter part is composed of continuous wood pulp fibers, the wood pulp fiber short diameter part is composed of wood pulp fibers with the length within the range of 35-150 mm, the content of the wood pulp fiber long diameter part is 95%, and the content of the wood pulp fiber short diameter part is 5%; the wood pulp tissue body is soaked with blended medicine liquid, and the blended medicine liquid comprises a bacterium remover, a corrosion remover, a thinner, an emulgator and humectant. According to the dissoluble cleaning tissue wholly made of the wood pulp, the proportion of the wood pulp fibers is changed, so that the elasticity of the wood pulp tissue is enhanced, the use comfort level is improved, the dissolving effect of the wood pulp tissue is better, and the wood pulp tissue is not likely to block a pipeline; the blended medicine liquid with the reasonable proportion is added so that the sterilizing and disinfecting effect of the wood pulp tissue can be better, and safety is high; broken lines are additionally arranged on the wood pulp tissue, and thus use is more convenient.
Owner:JIANGSU KANGLONG IND & TRADE CO LTD

Bamboo wood reinforcing and synergistic treatment method

The invention relates to a bamboo wood reinforcing and synergistic treatment method. A nano cellulose dispersion liquid is prepared by adopting a sulfuric acid hydrolysis method, nano-zinc oxide is generated on the surface of nano-cellulose in situ by taking the nano cellulose dispersion solution with a certain concentration as a precursor solution, and a cellulose dispersion liquid is compoundedand improved and is impregnated into pores of bamboo wood in a specific mode to fill, bond and reinforce wood tissues, so that the hardness, strength and size stability of the treated material are enhanced, and the mildew-proof and corrosion-proof performance is obviously improved. The method is suitable for reinforcing and synergistic treatment of loose wood such as fast-growing wood and man-madeforests, and is also suitable for reinforcing and repairing of mildly rotten wood.
Owner:ZHEJIANG FORESTRY ACAD

Method for extracting DNA from wood targeted cells

The invention discloses a method for accurately extracting DNA from wood targeted cells; through removal of exogenous pollution on a wood surface, in-situ detection of wood DNA, radial section of woodtissues, precise separation of the wood targeted cells enriched in DNA under an inverted microscope, cracking of wood cell walls, removal of a wood extract, DNA precipitation and adsorption, dissolution, purification, PCR amplification and other steps, DNA is extracted accurately from the wood tissues. The method aims at the wood targeted cells to carry out DNA accurate extraction, the quantity and purity of DNA extracted from the wood tissues are improved, and the interference of other types of cells of wood on DNA extraction is reduced. The method can be directly applied to study of molecular biology and provide a new technical support for identification of wood species.
Owner:INST OF WOOD INDUDTRY CHINESE ACAD OF FORESTRY

A rapid and efficient method for inducing the rooting of Binmei tissue culture seedlings

The invention discloses a rooting basal medium (BPM) and a cultivation method thereof, characterized in that the components of the MS basal medium are improved, rooting inducers (NAA, activated carbon) are added, and culture conditions are adjusted. , the rooting rate in the bottle can reach 100% in 14 days, so that the transplanting survival rate can reach more than 90%, which is 12%~34% higher than the current report. It only takes 45 days from inoculation to transplanting to survive, which not only improves the rooting efficiency of the tissue-cultured seedlings of Lime plum, but also has the characteristics of neat and robust seedlings, saving costs and high efficiency, and shortening the cultivation cycle of tissue-cultured seedlings of Lime plum. Chemical production provides the means to rapidly produce high-quality seedlings.
Owner:INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI

A kind of method for rapid propagation of tamarisk tissue culture

InactiveCN105052738BOvercoming Disinfection Browning ProblemsRealize large-scale productionHorticulture methodsPlant tissue cultureSucroseShoot
The invention relates to the technical field of wood tissue culture seedling raising in forest science, in particular to a Tamarix chinensis tissue culture and rapid propagation method. The method includes: acquisition of aseptic seedlings, with an involved medium containing 6-BA, IBA, GA, sucrose and agar; subculture: inoculating the aseptic seedlings into a subculture medium to conduct culture so as to obtain clustered shoots, with the medium containing ZT, IBA, sucrose and agar; rooting culture: inoculating the clustered shoots into a rooting medium containing IBA, NAA, GA, sucrose and agar to obtain rooted tissue cultured seedlings; and acclimatization and transplantation. The propagation method has high propagation coefficient and short propagation cycle, the obtained seedlings are strong and have a lot of root systems, and the survival rate of transplantation is over 96%. The method provided by the invention omits the strong seedling culture step in conventional methods, improves propagation efficiency, makes the Tamarix chinensis tissue culture and rapid propagation technology move towards production from laboratories, realizes large-scale production of Tamarix chinensis tissue culture seedlings, and provides technical support for promotion of Tamarix chinensis rapid propagation.
Owner:QINGDAO ZHENGJIE INDAL

Primary fir wood culture bud induction method

The invention discloses a primary fir wood culture bud induction method. The method comprises the following steps: inoculating an explant as a coppice shoot of a fir wood base part and subjected to sterile explant treatment to a bud induction culture medium subjected to high-quality primary culture, culturing under the conditions of proper temperature, humidity and illumination intensity, and inducing bud germination. The conventional fir wood induction technology is improved, the problems that the explant is subjected to browning and vitrifaction in the fir wood bud induction process and the like are solved, the fir wood bud induction rate, the budding index and the germination and growth conditions are improved, and large-number high-quality buds are obtained, so that the multiplication culture requirement is met, the establishment of a rapid fir wood tissue culture reproductive system is promoted, and the method has high economic benefits and social benefits.
Owner:GUANGXI FORESTRY RES INST

Method and tool for machining rod-shaped reconstructed wood unit

The invention discloses a method and a tool for machining a rod-shaped reconstructed wood unit. The method comprises the following steps of: cutting wood along the longitudinal direction of the fiber of the wood according to the set depth by using a scratching knife with more than two blades without sawdust; generating lateral separation and generating a scratching knife mark by the wood tissue with the set depth; cutting along the longitudinal direction of the wood by using the scratching knife blade in the direction vertical to the depth of the scratching knife mark; and cutting the wood toform a wood-wool rod. The middle boss of the base of a cutter is provided with dovetail grooves; one side surface of each dovetail groove is provided with a rectangular path; a plane cutter is arranged in the dovetail groove on the side, and the scratching knife is installed in the dovetail groove on the other side; the scratching knife and the plane cutter are fixed on two sides of the dovetail groove by press cakes; the scratching knife and the plane cutter are higher than the top surface of the boss; and a gap is formed between the inclined bottom surface of each press cake and the blade of the plane cutter. The wood-wool rod is easy to realize directional pavement when a wood assembly is reconstructed. The wood is cut longitudinally along the vertical grain without sawdust. The tool has the advantages of small noise, small cutting resistance, no dust pollution and high processing efficiency.
Owner:ZHEJIANG FORESTRY UNIVERSITY

A method for extracting DNA from wood-targeted cells

The invention discloses a method for accurately extracting DNA from wood targeted cells; through removal of exogenous pollution on a wood surface, in-situ detection of wood DNA, radial section of woodtissues, precise separation of the wood targeted cells enriched in DNA under an inverted microscope, cracking of wood cell walls, removal of a wood extract, DNA precipitation and adsorption, dissolution, purification, PCR amplification and other steps, DNA is extracted accurately from the wood tissues. The method aims at the wood targeted cells to carry out DNA accurate extraction, the quantity and purity of DNA extracted from the wood tissues are improved, and the interference of other types of cells of wood on DNA extraction is reduced. The method can be directly applied to study of molecular biology and provide a new technical support for identification of wood species.
Owner:INST OF WOOD INDUDTRY CHINESE ACAD OF FORESTRY

Tissue culture and rapid propagation culture medium and tissue culture and rapid propagation method for thin-bark wood

The invention belongs to the technical field of thin-bark wood tissue culture and rapid propagation, and particularly relates to a thin-bark wood tissue culture and rapid propagation culture medium and a thin-bark wood tissue culture and rapid propagation method, the thin-bark wood tissue culture and rapid propagation culture medium comprises a starting culture medium, a proliferation culture medium and a rooting culture medium, the proliferation culture medium takes the starting culture medium as a basic culture medium, and 0.5-3.0 mg of ZT is added into each liter of the basic culture medium; a rooting culture medium takes 1 / 2 MS as a basic culture medium, and 20-30 g of cane sugar, 5-7 g of agar, 0.1-0.5 mg of IBA and 0.05-0.1 mg of NAA are added into each liter of 1 / 2 MS. By utilizing the culture medium provided by the invention, the maximum multiplication coefficient can reach 6.03, and the rooting rate can reach 100%.
Owner:HENAN VOCATIONAL COLLEGE OF AGRI

A kind of acrocarp tissue culture method

The invention provides a tissue culture method of acrocarpus fraxinifolius. The tissue culture method comprises the following steps: step 1. selecting an explant; step 2. treating the explant; step 3. inoculating; step 4. producing an induction culture medium; step 5. producing a subculture medium; and step 6. culturing a strong seedling, wherein the production formula of the induction culture medium comprises 1mg / L of MS+6BA, 0.2mg / L of NAA, 30g of white sugar and 6.5g of agar, and the production and selection formula of the subculture medium comprises 0.6mg / L of MS+6BA, 0.2mg / L of NAA, 30g of white sugar and 6.5g of agar. According to the vegetative rapid reproduction method of acrocarpus fraxinifolius, three different culture mediums are used for culturing acrocarpus fraxinifolius, and plants with ordered appearance can be reproduced in a large scale in relatively short time, and the variation of the regenerated plants is low; a regeneration system is very efficient; and the average cost of a single regenerated seedling is extremely low, the large-batch efficient and rapid reproduction of acrocarpus fraxinifolius is facilitated, so that the tissue culture method is used for industrial production.
Owner:JIANGMEN XINHUI FORESTRY SCI RES INST

Tissue Culture and Rapid Breeding Method of Pig Bloodwood

The invention provides a tissue culture and rapid propagation method of euryodendron excelsum. The method comprises the following steps: selecting and disinfecting explants, inducing clustered buds, proliferating clustered buds, rooting and culturing, culturing strong seedlings and culturing a seedling bed. Compared with the conventional seedling-raising method of euryodendron excelsum, the method does not adopt euryodendron excels seeds; the new sprout seedlings of the euryodendron excelsum are adopted; few plant raw materials are needed; the euryodendron excelsum is subjected to short-time and mass propagation; the heritable variation of the variety is low; the young seedlings is high in quality; the survival rate of the young seedlings reaches up to 90%; the survival rate of transplanted seedlings reaches up to 80%; after transplanting, the seedlings are excellent in growth vigor; the phenomenon of small seedling is avoided; the problems that the euryodendron excelsum wild resources are increasingly reduced and natural reproduction of the population is difficult can be effectively solved; the technical support is provided for effective protection of euryodendron excelsum serving as a rare and threatened plant.
Owner:红谷滩建设集团有限公司

A kind of rapid propagation method of Tongdao wood tissue culture

The invention provides a rice paper plant tissue culture rapid breeding method, and belongs to the technical field of agriculture planting. The method includes the steps of conducting surface disinfection, callus inducement, bud differentiation culture and multiplication, rooting induction, plant exercising and transplantation with rice paper plant young leaves or young stems or young buds as explant. The tissue culture rapid breeding technical system for rice paper plants is established for the first time, the operation method is easy, convenient and feasible, the breeding coefficient is high, 20 times to 25 times of multiplication can be achieved every 60 days, the tissue culture seedling rooting rate reaches 98% or above, the plant exercising and transplanting survival rate reaches 92% or above, and the large-scale production can be conducted.
Owner:HUNAN AGRICULTURAL UNIV

Novel excellent flower spring blood wood tissue culture rapid propagation method

The invention relates to the technical field of plant tissue culture, and discloses a novel excellent flower spring blood wood tissue culture rapid propagation method. The method comprises the steps of explant pretreatment, explant surface disinfection, bud induction, acquisition of tufty adventitious buds, rooting culture and seedling hardening and transplanting. The method has the advantages ofrapid axillary bud induction, a high induction rate, a large proliferation multiple, thick and strong tufty buds, rapid elongation and growth of adventitious buds, a high rooting rate, a developed root system, a thick and strong rooting seedling, a high transplanting survival rate, healthy and strong seedling growth, low planting management cost and the like, a stable and efficient spring blood wood branch stem vitro plant regeneration method is also provided, the cultured spring blood wood seedling is suitable for large-scale production, healthy and strong spring blood wood with unfolded leaves can be cultured, the import cost of spring blood wood seeds is reduced, and the significance for improving the ornamental value of the spring blood wood is important.
Owner:海南省林业科学研究所

A kind of tissue culture disinfection and sterilization formula and tissue culture method of flower palm wood

The invention discloses a tissue culture disinfection and sterilization formula of ormosia henryi prain and a tissue culture method of the ormosia henryi prain. Seed seedling stems are disinfected by 70% ethanol for 30 seconds, sterilized by 0.1% mercury bichloride for 8 minutes, disinfected by the 70% ethanol for 30 seconds and treated by 0.5ml / L of a plant tissue culture antibacterial agent to achieve the best disinfection effect; the optimal collection time of seedling explants is April; the stems are immersed by the 70% ethanol for 30 seconds, immersed by a mixed solution (containing 50mg / L rifampicin and 50mg / L chloramphenicol) for 30 minutes and immersed by 0.1% mercury bichloride for 8 minutes to achieve the best disinfection effect; and leaves are immersed by the 70% ethanol for 30 seconds, immersed by the mixed solution (containing 50mg / L rifampicin and 50mg / L chloramphenicol) for 30 minutes and immersed by 0.1% mercury bichloride for 6 minutes to achieve the best disinfection effect. Leaves of grown plants are relatively suitable for collecting in April, and are immersed by the 70% ethanol for 30 seconds, immersed by the mixed solution (containing 50mg / L rifampicin and 50mg / L chloramphenicol) for 30 minutes and immersed by 0.1% mercury bichloride for 12 minutes. According to the tissue culture disinfection and sterilization formula and the tissue culture method, seed seedlings, seedlings and grown plant tissues of ormosia henryi prain explants are disinfected by different disinfection methods, and the relatively good survival rate can be obtained; and sources of ormosia henryi prain tissue culture multiplication materials are greatly expanded, and the tissue culture effect of the ormosia henryi prain can be greatly improved by controlling disinfection time.
Owner:GUIZHOU UNIV

A method for tissue culture and rapid propagation of plant regeneration induced by stem segments of North American Sequoia

The invention discloses a sequoia sempervirens steam section induction plant regeneration tissue culture rapid propagation method, and belongs to the technical field of forest wood tissue culture breeding. The method belongs to a propagation method characterized in that the screened sequoia sempervirens high-quality plants are used as female parents; the annual stems with buds are selected to be used as explants for inducing normal bud germination; then, aseptic seedlings are used for induction to generate adventitious buds; then, the adventitious buds are used for inducing regeneration plantsto root for seedling domestication and transplanting potting. The sequoia sempervirens fast breeding technology is used for mass production; the method is simple and convenient; the regeneration period is short; the propagation coefficient is high; the transplanting survival rate is high; the seedling germination is regular and consistent; the maintenance and management are convenient; the laborand land resources are saved; the production cost is low; the season limitation is avoided; the regeneration plants are continuously obtained; the method can be used for seedling industrialized production.
Owner:CHINA THREE GORGES CORPORATION

A method for detecting microorganisms in wood

The invention discloses a method for detecting microorganisms in wood. The method for detecting microorganisms in wood of the present invention first needs to pretreat the wood in a constant temperature and humidity box to keep the moisture content at about 20%, and then after the surface is sterilized, the sawdust is drilled quantitatively and at a certain depth, and after adding β-mercaptoethanol The dodecyltrimethylammonium bromide (DTAB) solution was cracked to extract the total microbial genetic material in the wood tissue cells, and then the microbial information in the wood was obtained through amplification and sequencing, and the microbial species in the wood were determined. Compared with the traditional separation method, the method of the present invention can obtain more complete wood microbial information, which is beneficial to the effective prevention and control of wood decay or the degradation of wood, and effectively makes up for the fact that the current detection of wood microbes cannot be separated from separation, purification and The shortcomings of the traditional method of tissue culture provide a new test method for the biodegradation and control of wood.
Owner:INST OF WOOD INDUDTRY CHINESE ACAD OF FORESTRY +1

Lines embedding method for wood or wood composite floor board

The invention relates to a method for planting patterns of solid wood or solid wood composite flooring. It comprises the following steps: a. Sanding the wooden floor plain board or light board blank; b. Coating the surface of the wooden floor plain board or light board blank; c. Using a material with a certain shape on the wooden floor plain board Or make texture traces on the surface of the bare board blank material; d. Dry the wooden floor plain board or light board blank material after the texture traces are made and the paint is applied. After adopting the above method, wood tissue, planted texture and paint can be integrated into a unified whole, and when the wood tissue is further hardened and shaped, texture with natural color and luster can be obtained.
Owner:毛伟锋

Extraction method of santalum album high-content essential oil tissue total RNA

The invention discloses a method for extracting santalum album high-content essential oil tissue total RNA. The method for extracting the santalum album high-content essential oil tissue total RNA comprises the following steps: grinding santalum album wood tissue fluid in liquid nitrogen so as to obtain powder; adding the powder into Tris-HCl extraction liquid, carrying out freezing treatment at -80 DEG C, and carrying out centrifuging so as to obtain liquid supernatant; adding sodium acetate and isopropanol into the liquid supernatant so as to have RNA precipitated, carrying out centrifuging,and dissolving the precipitates by using a TE buffer solution; adding cetyltrimethylammonium bromide into the dissolved precipitates, carrying out uniform mixing, carrying out extraction by using chloroform / isoamyl alcohol, and centrifuging the extract so as to obtain liquid supernatant; and then, adding lithium chloride into the liquid supernatant so as to carry out RNA precipitation overnight, centrifuging the precipitated RNA, and washing the centrifuged RNA by using ethanol so as to obtain the total RNA. Being tested by using Nanodrop, the santalum album wood tissue total RNA extractedby the method disclosed by the invention has an OD<260> / OD<280> ratio of 2.0 or above, an OD<260> / OD<230> ratio of 2.3 or above, an RNA concentration in aqueous solution of 800.0 ng / [mu]L or more,and RIN higher than 7.5-1; and 1.0% denaturing agarose gel electrophoresis shows that the RNA strip is clear and good in integrity, so that the santalum album wood tissue total RNA has the advantagesof being high in quality and high in concentration.
Owner:SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI

A kind of composite material of titanium dioxide and charcoal and preparation method thereof

The invention discloses a composite material of titanium dioxide and charcoal and a preparation method thereof, and belongs to the field of semiconductor photocatalysts; the microstructure of the carbon in the composite material of titanium dioxide and charcoal maintains the structure of wood tissue, and the titanium dioxide is uniformly dispersed on the surface of the charcoal. Titanium dioxide has both anatase and rutile phases; the method first pretreats the woody biological template; then the woody biological template is impregnated in the precursor solution, and the TiO 2 Particles are deposited in the micropores of the wooden formwork to obtain a woody biological template with titanium dioxide deposited, and finally roasted in a muffle furnace to obtain a titanium dioxide / charcoal composite photocatalyst; the composite material of titanium dioxide and charcoal prepared by the present invention can be used to remove The adsorption removal rate of environmental endocrine disruptors and other organic pollutants in water is 32.3-86.5%, the photocatalytic removal rate is 11-20%, and the total adsorption / photocatalytic synergistic removal rate can reach 50.3-97.5%. Good application prospects.
Owner:KUNMING UNIV OF SCI & TECH

A kind of tissue culture rapid propagation method of Chongyang wood

The invention provides a method for tissue culture and fast propagation of Chongyang wood, which uses Chongyang wood with axillary bud stem segments or Chongyang wood leaves as explants for tissue culture propagation. When the explants are Chongyang wood with axillary bud stem segments, the cultivation method is: sterilize the bud stem segments, then place the bud stem segments in the induction medium to directly obtain clump seedlings, and then cut the clump seedlings into individual seedlings and inoculate them one by one. Rooting culture is carried out on the rooting medium, and finally the seedlings after rooting and culture are refined and transplanted in sequence, so as to realize the rapid propagation of Chongyang tree. When the sterile leaves of Chongyang wood are used as explants, the culture method is as follows: sterilize Chongyang wood seeds, and cultivate the sterile leaves, then use the sterile leaves as the explants, and sequentially induce callus. , Callus differentiation, rooting culture process, the establishment of Chongyang wood tissue culture rapid propagation system. The invention has the advantages of simple operation, low cultivation cost, high reproduction efficiency, little influence by natural environment, and good market and industrial application prospect.
Owner:ANHUI AGRICULTURAL UNIVERSITY

A kind of blood wood tissue culture method

The invention discloses a method for culturing bloodwood tissue. The present invention uses the hypocotyls of the blood tree seeds as explants to successfully carry out tissue culture and rapid propagation of the high-quality seedlings of the blood tree of the excellent strain, and the produced seedlings of the blood tree have high genetic stability, Rapid emergence, strong seedlings, good seedling quality, strong resistance, good growth and other characteristics; it has a good market prospect.
Owner:SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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