Vitis amurensis Rupr. tissue culture medium and Vitis amurensis Rupr. tissue culture method

A technique of tissue culture medium and tissue culture, applied in the field of plant tissue culture, to achieve the effects of controlling vitrification, facilitating proliferation and culture, and reducing the degree of vitrification

Inactive Publication Date: 2016-05-25
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, there are few reports on how to control the vitrification of Vitis vine tissue culture seedlings

Method used

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  • Vitis amurensis Rupr. tissue culture medium and Vitis amurensis Rupr. tissue culture method
  • Vitis amurensis Rupr. tissue culture medium and Vitis amurensis Rupr. tissue culture method
  • Vitis amurensis Rupr. tissue culture medium and Vitis amurensis Rupr. tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The mountain grape variety Beibinghong was used as the test material, which was obtained from the National Fruit Tree Germplasm Zuojia Mountain Grape Resource Garden of the Institute of Special Products, Chinese Academy of Agricultural Sciences. Take the branches of Vitis vinifera and carry out germination culture in water, cut off the 1-year-old new shoots with 4 to 5 leaves, remove the leaves, cut into single-bud stem sections about 2cm long, and then put these single-bud stem sections into a small amount Shake in washing powder water for 10 minutes, rinse with running water for 20 minutes, disinfect the surface with 0.1% mercuric chloride on the ultra-clean workbench for 3 minutes, rinse with sterile water for 5 times, and cultivate according to the following steps. The specific steps are as follows:

[0054] (1) Induction culture: Inoculate the 2cm-long single-bud stem section of Vitis vinifera after disinfection and washing into the induction medium, and the culture...

Embodiment 2

[0064] The mountain grape variety Zuoyouhong was used as the test material, and the material was obtained from the National Fruit Tree Germplasm Zuojia Mountain Grape Resource Garden of the Institute of Special Products, Chinese Academy of Agricultural Sciences, and the mountain grape seedlings were cultivated according to the method steps described in Example 1.

[0065] Wherein, during the culturing process of Vitis vine seedlings in this example, the conditions for the induction culture are 23°C room temperature, 16h / d light hours, 2500Lx light intensity, and 50% indoor humidity, and the induction culture time is 25 days.

[0066] In the induction medium, the content of each component is 0.5 mg / L of 6-benzylaminopurine, 0.001 mg / L of naphthaleneacetic acid, 7.5 g / L of agar, 30 g / L of sucrose, and 0.8 g / L of activated carbon. L, the pH of the induction medium was 6.0.

[0067] In this example, the culture medium and culture conditions used in the rest of the culture steps ar...

Embodiment 3

[0069] The mountain grape variety Shuanghong was used as the test material, and the material was obtained from the National Fruit Tree Germplasm Zuojia Mountain Grape Resource Garden of the Institute of Special Products, Chinese Academy of Agricultural Sciences, and the mountain grape seedlings were cultivated according to the method steps described in Example 1.

[0070] Wherein, in the cultivation process of Vitis vine seedlings in this embodiment, the conditions for the transition culture are: transfer the transition medium to the bottom of the culture rack, turn off the fluorescent lamp, so that the transition medium does not receive light, and store the transition medium at room temperature 25°C. , and the indoor humidity was controlled at 50% for transitional cultivation for 10 days.

[0071] The transition medium is 1 / 2 MS basic medium, in which 6-benzylaminopurine, naphthaleneacetic acid, agar, sucrose are dissolved, and activated carbon is added. In the transition med...

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Abstract

The invention provides a Vitis amurensis Rupr. tissue culture medium. In the culture medium, the water potential of the culture medium is reduced by increasing the concentrations of substances including agar, sucrose and the like in the culture medium, and thus a Vitis amurensis Rupr. tissue culture seedling vitrification phenomenon, particularly a vitrification phenomenon at an induction phase, is alleviated to a certain extent. Meanwhile, active carbon is added into the culture medium so that a tissue culture seedling browning phenomenon caused by the fact that the concentration of the sucrose in the culture medium is increased can be avoided, and furthermore, the success rate of Vitis amurensis Rupr. tissue culture is improved. Meanwhile, the invention further provides a Vitis amurensis Rupr. tissue culture method. According to the method provided by the invention, a transition culture step is further added to an existing seedling tissue culture process, and the culture medium provided by the invention is used as an induction culture medium and a multiplication culture medium, so that the vitrification phenomenon in a Vitis amurensis Rupr. induction culture process and a Vitis amurensis Rupr. multiplication culture process is effectively controlled by optimizing a culture flow and the used culture medium.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a vine tissue culture medium and a vine tissue culture method. Background technique [0002] Mountain grape (Vitisamurensis Rupr.) is native to Northeast China, North Korea, and the Far East of the former Soviet Union. Mountain grapes are also the main raw materials for the wine-making industry in Northeast my country. The wines produced are bright and transparent, and have unique flavors, so they are very popular among consumers. At the same time, Vitis vinifera is also the most cold-resistant species in the genus Vitis, and it also has strong resistance to white rot, powdery mildew, anthracnose and black bean disease. Therefore, it is an important germplasm resource for cold-resistant and stress-resistant grape breeding, and has high economic value. [0003] The Institute of Specialty Products of the Chinese Academy of Agricultural Sciences has established a national fruit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 赵滢艾军李昌禹秦红艳张宝香杨义明范书田王振兴许培磊刘迎雪孙丹王春伟
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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