Vitis amurensis Rupr. tissue culture medium and Vitis amurensis Rupr. tissue culture method
A technique of tissue culture medium and tissue culture, applied in the field of plant tissue culture, to achieve the effects of controlling vitrification, facilitating proliferation and culture, and reducing the degree of vitrification
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Embodiment 1
[0053] The mountain grape variety Beibinghong was used as the test material, which was obtained from the National Fruit Tree Germplasm Zuojia Mountain Grape Resource Garden of the Institute of Special Products, Chinese Academy of Agricultural Sciences. Take the branches of Vitis vinifera and carry out germination culture in water, cut off the 1-year-old new shoots with 4 to 5 leaves, remove the leaves, cut into single-bud stem sections about 2cm long, and then put these single-bud stem sections into a small amount Shake in washing powder water for 10 minutes, rinse with running water for 20 minutes, disinfect the surface with 0.1% mercuric chloride on the ultra-clean workbench for 3 minutes, rinse with sterile water for 5 times, and cultivate according to the following steps. The specific steps are as follows:
[0054] (1) Induction culture: Inoculate the 2cm-long single-bud stem section of Vitis vinifera after disinfection and washing into the induction medium, and the culture...
Embodiment 2
[0064] The mountain grape variety Zuoyouhong was used as the test material, and the material was obtained from the National Fruit Tree Germplasm Zuojia Mountain Grape Resource Garden of the Institute of Special Products, Chinese Academy of Agricultural Sciences, and the mountain grape seedlings were cultivated according to the method steps described in Example 1.
[0065] Wherein, during the culturing process of Vitis vine seedlings in this example, the conditions for the induction culture are 23°C room temperature, 16h / d light hours, 2500Lx light intensity, and 50% indoor humidity, and the induction culture time is 25 days.
[0066] In the induction medium, the content of each component is 0.5 mg / L of 6-benzylaminopurine, 0.001 mg / L of naphthaleneacetic acid, 7.5 g / L of agar, 30 g / L of sucrose, and 0.8 g / L of activated carbon. L, the pH of the induction medium was 6.0.
[0067] In this example, the culture medium and culture conditions used in the rest of the culture steps ar...
Embodiment 3
[0069] The mountain grape variety Shuanghong was used as the test material, and the material was obtained from the National Fruit Tree Germplasm Zuojia Mountain Grape Resource Garden of the Institute of Special Products, Chinese Academy of Agricultural Sciences, and the mountain grape seedlings were cultivated according to the method steps described in Example 1.
[0070] Wherein, in the cultivation process of Vitis vine seedlings in this embodiment, the conditions for the transition culture are: transfer the transition medium to the bottom of the culture rack, turn off the fluorescent lamp, so that the transition medium does not receive light, and store the transition medium at room temperature 25°C. , and the indoor humidity was controlled at 50% for transitional cultivation for 10 days.
[0071] The transition medium is 1 / 2 MS basic medium, in which 6-benzylaminopurine, naphthaleneacetic acid, agar, sucrose are dissolved, and activated carbon is added. In the transition med...
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