Novel culture medium for rapid propagation of peony

A culture medium and rapid technology, applied in tissue culture, plant cells, microorganisms, etc., can solve the problems of low reproduction coefficient of peony seedlings, small root quality, and difficulty in rooting seedlings, and achieve the advantages of rapid division, strong rooting, strong growth effect

Inactive Publication Date: 2014-06-25
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The technical problem to be solved by the present invention is: to provide a peony rapid propagation medium, which can effectively solve the problems of low reproductive coefficient of peony seedlings, difficult rooting of seedlings, small root quality and difficult transplanting, and can be used for large-scale production of peony. Provide large quantities of peony seedlings

Method used

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Embodiment Construction

[0020] The present invention will be described in further detail below in conjunction with specific embodiment, a kind of peony rapid propagation culture medium, comprises macroelement, microelement, iron salt and organic matter,

[0021] The macroelements consist of 300-1200 mg / L NH 4 NO 3 , 1400 mg / L KNO 3 , 150 mg / L CaCl 2 2H 2 O, 150 mg / L MgSO 4 ·7H 2 O and 400-800 mg / L KH 2 PO 4 Composition, KH 2 PO 4 The concentration is preferably 650 mg / L, NH 4 NO 3 The concentration is preferably 750-1200 mg / L;

[0022] The trace elements consist of 0.83 mg / L KI, 6.2 mg / L H 3 BO 3 , 11.2 mg / L MnSO 4 4H 2 O, 8.6 mg / L ZnSO 4 ·7H 2 O, 0.025 mg / L CuSO 4 ·5H 2 O and 0.025 mg / L CoCl 2 ·6H 2 O composition;

[0023] The iron salt is composed of 27.85 mg / L FeSO 4 ·7H 2 O and 37.25 mg / L Na 2 -EDTA·2H 2 O composition;

[0024] The organic matter consists of 0.5 mg / L niacin, 0.5 mg / L pyridoxine hydrochloride, 1 mg / L thiamine hydrochloride, 2 mg / L glycine, 60-90 g / L sucr...

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Abstract

The invention discloses a novel culture medium for rapid propagation of peony and relates to a new formula of a culture medium. The culture medium comprises macro-elements, trace elements, iron salt, organic matters and distilled water and is formed by a great improvement on the basis of a traditional MS culture medium. The novel culture medium (LPM) for rapid propagation of peony is especially suitable for tissue culture of peony; and rapidly propagated peony seedlings grow strongly, have a high propagation coefficient, and are thick and strong in root, large in quantity and strong in activity, and the transplanting survival rate of the seedlings is up to more than 85%.

Description

technical field [0001] The invention relates to a new formula of a culture medium, in particular to a peony rapid propagation medium (LPM). Background technique [0002] Peony is a traditional famous flower in my country. It is beautiful in color and fragrant, and has high ornamental value. The root bark is a precious Chinese medicinal material, which has the functions of clearing away heat and cooling blood, promoting blood circulation and removing blood stasis, and nourishing blood. The traditional propagation methods of peony include seed propagation in sexual propagation, branch propagation, layering propagation, grafting propagation in asexual propagation, etc. However, these propagation methods take a long time and can only be reproduced in the growing season. The growth of peony seedlings is small, the propagation speed is slow, and the insufficient number of seedlings is an important bottleneck restricting the large-scale expansion of peony production. Tissue cultur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04C12N5/00A01H4/00
Inventor 史国安高双成施江王世华李玉龙
Owner HENAN UNIV OF SCI & TECH
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