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Method for Overcoming Vitrification of Carnation Stem Tip Culture by Using High Molecular Absorbent Resin

A polymer water-absorbing and carnation technology, applied in gardening methods, botanical equipment and methods, applications, etc., can solve the problems of insignificant effect and ineffective effect of carnation red varieties, and achieve the effect of reducing humidity in the bottle

Active Publication Date: 2018-01-16
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But it has little effect on carnation red varieties, and it has no obvious effect on other color varieties, and has no effect on the vitrification phenomenon of carnation virus-free seedlings.

Method used

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  • Method for Overcoming Vitrification of Carnation Stem Tip Culture by Using High Molecular Absorbent Resin
  • Method for Overcoming Vitrification of Carnation Stem Tip Culture by Using High Molecular Absorbent Resin

Examples

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Effect test

Embodiment 1

[0028] 1) Select "Master" carnation plants with strong growth, good quality and no pests and diseases, select strong lateral buds at the base, peel off the unfolded leaves, leave 2-3 pairs of leaves unexpanded, and cut off the 2cm stem section with the top , placed in a clean beaker for later use;

[0029] 2) Sterilize on an ultra-clean workbench, pour 0.1% mercury liter solution, and shake gently to make the plant material contact with the sterilized liquid evenly. After 8 minutes of sterilization, rinse with sterile water 3-5 times. spare;

[0030] 3) Under the dissecting microscope, 0.5mm of carnation stem tip is stripped and inserted into a 100ml bottle with 40ml / bottle of culture medium. L+NAA1g / L+0-24g / L high molecular weight water-absorbing resin, after receiving 4 plants in each bottle, cultivate them at 26℃±0.5℃, light intensity 1000lx-4000lx, from August 10, 2013 to August 30 day, statistical results;

[0031] Specifically, a total of 4 treatments were made with g...

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Abstract

The invention belongs to the field of plant regeneration, and provides a method for overcoming the vitrification of carnation stem tip cultivation by using high-molecular water-absorbing resin, comprising the steps of: 1) selecting a robust carnation plant, and cutting off a 2 cm stem segment with a top; 2) Rinse with sterile water 3-5 times and set aside; 3) Peel off 0.5mm of carnation stem tip and insert it into the culture medium, the culture medium is: iron salt doubled MS+sucrose 30g / L+agar 6g / L+TDZ1g / L +NAA1g / L+8g / L polymer water-absorbent resin, and then cultivated at 20-30°C. The present invention determines the selection of medium additive species through tests, and selects the concentration of the additives through test comparison. According to the test results, the polymer water-absorbing resin with suitable concentration can reduce the incidence of vitrification to about 17% after the detoxification of the stem tip of carnation red variety, and the subsequent vitrification of the non-vitrified plants is also greatly reduced. reduce.

Description

technical field [0001] The invention belongs to the field of plant regeneration, and in particular relates to a tissue culture method for overcoming the vitrification of shoot tip culture. Background technique [0002] Vitrification, another common problem in tissue culture, is a physiological disorder of tissue culture plantlets. When plant materials are continuously propagated in vitro, the tender stems and leaves of some cultures tend to be translucent and water-soaked. This phenomenon is usually called vitrification. It will cause the growth of tissue cultured seedlings to be slow or deformed, the reproduction coefficient will be reduced, and the seedlings cannot be transplanted to survive. It occurs in both herbaceous and woody plants, and it has been reported that more than 70 plants have glass seedlings. The leaves of glass seedlings are crystal transparent or translucent, water-soaked, the plants are short and swollen, the color is green, the leaves are shrunken an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 刘春彭佳佳杨哲李佩愉明军袁素霞
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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