35 results about "Plant reproduction" patented technology

Methods are described herein for producing hybrid seed using male-sterile plants wherein reproductive function is restored by treatment of mutant plants with selected plant hormones. Plants comprising mutations in the allene oxide synthase gene are functionally male-sterile and are useful for the production of hybrid seed, wherein fertility is restored in the plants by treatment with jasmonic acid. Hybrid seed production is simplified and improved by this invention. The present invention also includes plants comprising mutations in genes affecting allene oxide synthase and / or hydroperoxide lyase.

Solanum viarum is an alkaloid producing plant of the family Solanaceae with varied therapeutic uses. In order to grow the plant in large areas; one needs to have an efficient system of vegetative multiplication, which ensures its genetic uniformity, and true to the type nature. In nature largely seeds propagate plant, which could be result of cross-pollination which may result in genetic drift. The present invention provides an efficient micropropagation system, with high level of multiplication at relatively low cost of production. The multiplication ratio was as high as 1:6 and almost 95% the plants were viable and successfully cultivated in field. The present invention provides an ideal way of mass cultivation of the selected elite plant material.

The invention provides an isolated culture of a Neotyphodium endophyte of an Elymus canadensis host plant, wherein the endophyte reproduces asexually and enhances the agronomic characteristics of the host plant. Methods for inoculating the host plant with the endophyte, for propagating the host-endophyte combination, and for detecting the presence of the endophyte and of its metabolites within a host plant are also described.

The invention belongs to the field of plant regeneration, and provides a method for overcoming the vitrification of carnation stem tip cultivation by using high-molecular water-absorbing resin, comprising the steps of: 1) selecting a robust carnation plant, and cutting off a 2 cm stem segment with a top; 2) Rinse with sterile water 3-5 times and set aside; 3) Peel off 0.5mm of carnation stem tip and insert it into the culture medium, the culture medium is: iron salt doubled MS+sucrose 30g / L+agar 6g / L+TDZ1g / L +NAA1g / L+8g / L polymer water-absorbent resin, and then cultivated at 20-30°C. The present invention determines the selection of medium additive species through tests, and selects the concentration of the additives through test comparison. According to the test results, the polymer water-absorbing resin with suitable concentration can reduce the incidence of vitrification to about 17% after the detoxification of the stem tip of carnation red variety, and the subsequent vitrification of the non-vitrified plants is also greatly reduced. reduce.

The invention discloses a culture medium for tissue culture and rapid propagation of heteromorphic Jade leaf golden flower and a culture method, and belongs to the technical field of plant propagation. The present invention adopts the tender stem of the special-shaped Jade leaf golden flower as the explant. After the explant is sterilized, a large amount of callus is produced through the induction medium, and the callus will be differentiated after being transferred to the subculture medium for 3 to 4 times. Buds are produced, and when the plants grow to 2-3 cm, they are divided into individual plants and transferred to the proliferation medium. After 30 days of cultivation, a large number of clustered buds will be induced, and then independent and complete plants will be grown through the rooting medium. The culture medium formula provided by the invention can induce differentiation of the heteromorphic Jade-leaf flower to produce a large number of plants, and solve the dilemma that the heteromorphic Jade-leaf flower is facing endangerment.

The invention discloses a method for acquiring stamen motion track, comprising the following steps: A1: delineate the stamen area from the video image of flower blooming, and determine the type and number of stamens; A2: acquire stamens for the selected stamen area The value of the RGB component and the HIS component of the area; A3: perform statistics according to the RGB component and the HIS component of the acquired stamen area; A4: segment the stamen pixel according to the grayscale statistical peak value; A5: segment the stamen pixel and determine in step A1 Carry out cluster analysis on stamen category and number, and calculate the geometric center of each stamen in the image coordinate system; A6: Repeat steps A2 to A5 for subsequent frames of images; A7: According to each frame obtained in steps A5 and A6 The geometric center of stamens in the image coordinate system, and the trajectory of each stamen in the image coordinate system is obtained. Through the method of the invention, the trajectory of each stamen is tracked, and the pollination process of the stamen is studied, so that information with guiding significance for plant propagation and breeding can be obtained.

The invention discloses a rapid division propagation method of saliva, which comprises the following steps: 1) selecting the saliva and drying it in the sun; 2) cleaning the soil; 3) antiseptic treatment; 4) soaking the rhizome of the antiseptic treatment 2 to 4 hours in the plant growth regulator; 5) cutting and planting in the culture medium; 6) cultivating to obtain the germinated and grown Sagittarius. First of all, the present invention avoids the problem of the lack of large-scale reproduction of S. sliver because of the scarcity of seeds of S. sliver. At the same time, the germination rate of s. Mutation loss of the grass occurs, and the propagation speed is obviously improved, so that the amount of seedlings of the rare Yi medicine Sagittarius can be increased, and the number of germplasm resources of Sagittarius can be expanded. At the same time, the cost can be reduced, the operation is simple, and the economic benefit is high.

The invention discloses a rapid garden establishment method for grafting and field planting of kiwi fruit trees, and discloses a set of systematic garden establishment method comprising the steps of modifying a garden land, selecting and storing seed branches, selecting stocks, preparing to graft, grafting and carrying out field planting. Compared with the prior art, the rapid garden establishment method has the following advantages that (1) a direct grafting and field planting method is adopted, and a management procedure of breeding seedlings in a nursery garden is avoided; a garden is formed one year ahead, and the method is suitable for a newly built kiwi fruit tree garden with soil which is sufficiently improved, so that time and labor are saved; (2) the distance between plants in the field planting process of grafting seedlings is relatively reasonable, and a space in front of the plants is large, so that the development of nursery-grown plants is facilitated; (3) only new kiwi fruit seed branches and wild seedling stocks need to be introduced in winter, so that the propagation of plant diseases and insect pests in the nursery garden in a nursery-grown plant reproduction process is reduced; the occurrence probability of the plant diseases and insect pests is reduced, and the quality of the nursery-grown plants is improved; (4) the management level is relatively high, and fertilizers and water are sufficient; after the nursery-grown plants are germinated, the nursery-grown plants can grow quickly and be sold in the same year, so that rapid ripening of gardens is facilitated.

The present invention provides a method of regenerating a plant, which allows stable regeneration of plants from calli; and a method of reproducing a plant, which allows stable reproduction of plants without being affected by weather, seasons or other factors. The present invention relates to a method of regenerating a plant, including a step of inducing adventitious embryos from calli; and a method of reproducing a plant, including a step of inducing adventitious embryos from calli.

The invention discloses a nurse graft molding method of lythraceae lagerstroemia trunks. The method mainly includes the steps: (1) cultivating nurse graft nursery-grown plants; (2) selecting trunk nurse graft molding ways; (3) carrying out nurse graft on the nursery-grown plants; (4) manufacturing and installing a supporting frame manually; (5) manufacturing a molding supporting template and binding the nurse graft trunks; and (6) planting the molding nurse graft nursery-grown plants. The nurse graft molding method is suitable for nurse graft molding of the lythraceae lagerstroemia trunks, can carry out nurse graft molding of the trunks no matter whether affinity is strong or weak, and healing is fast or slow, overcomes the defect that a nursery-grown plant nurse graft method can only carry out nursery-grown plant reproduction on breeds with grafts and stocks weak in the affinity and slow in healing, can carry out nurse graft on a plurality of connecting ports and connecting ports long in length of the nurse graft trunks, achieves nurse graft molding of the trunks, and overcomes the defects that the nursery-grown plant nurse graft method can only carry out the nurse graft on one connecting port and the connecting ports short in length for finishing head exchanging of single-plant nursery-grown plant breeds, and the purpose of modification of trunk shapes is not achieved.

The invention discloses a method for raising seedlings of plants of the subgenus Azalea by using immature embryos. In the method, immature embryos aged 5 months (and above) after 5 months of pollination are taken, and subjected to GA 3 After pretreatment, the seedlings are sown in the greenhouse, and the specific steps include: (1) Stripping of young embryos, (2) Pretreatment of young embryos, (3) Sowing, (4) Emergence management, (5) Seedling management and transplanting . The invention shortens the time from pollination to seedling formation of subgenus azalea, thereby effectively shortening the breeding and growth cycle of subgenus azalea, and provides a simple and easy new way for breeding and seedlings of subgenus azalea, which is simple to operate, low in cost, and can be It is popularized and applied in the introduction and domestication of plants of the subgenus Azalea, hybrid breeding, and seedling production.