35 results about "Plant reproduction" patented technology

The present invention relates to the field of plant reproduction and seedling propagation technology, and is characterized by using special-made nutrient liquor and spraying it on the foliage of cuttage on the culture medium so as to promote the cutting quick grow. Said invention is low in cost, and high in reproduction efficiency.

The invention discloses a foliage spraying preparation for promoting fruit setting and fruit swelling and enhancing crop stress resistance. The preparation comprises the ingredients including fulvic acid, DA-6, 2,4-D sodium salt, alpha-pimacol, gibberellin, zeatin, dimethyl sulfoxide, butylcellosolve, sodium tetraborate, zinc sulfate, ferrous sulfate, molybdenum sulfate and magnesium sulfate. After the preparation is sprayed, nutrient substances are rapidly transferred from blade surfaces and are gathered at the plant reproduction active part, and the center endogenous hormones level of fruits is improved; the cell division is promoted, the cell size is amplified, the fruit swelling is accelerated, the chloroplast development and the chlorophyll formation are promoted, the photosynthetic efficiency of leaves is improved, and the unit area yield is increased; the flowing of nutrient substances to female flowers is enhanced, the fruit development speed is accelerated, the uniform fruit development is controlled, the deformity is reduced, the fruit surface is kept fresh, bright and smooth, and the commodity rate is obviously improved; and the immunological competence of plants are excited, the resistance is improved, the occurrence of the pest and disease damage is reduced, the senescence is delayed, and the picking period and the fruit shelf period are prolonged.

The invention discloses a method for performing regeneration propagation on sterile stalks of butterfly orchids, relates to the technical field of seedling cultivation, mainly relates to a quick butterfly orchid plant reproduction technology, and in particular relates to a plant regeneration method for the butterfly orchid species. The method comprises the steps of cutting a stalk axillary bud stem with a certain length from a robust mother butterfly orchid plant, taking the stalk axillary bud stem as an explant material, wherein an axillary bud is opened and a leaf is in jade green and glossy after the sterile stalk is subjected to primary culture; after the axillary bud is cut down, culturing the stalk on a subculture medium, then continuously forming bud points at an incision of the axillary bud of the stalk to gradually form another axillary bud, continuing to cut down the axillary bud after the axillary bud is opened, and inoculating the stalk on the subculture medium again for subculture, wherein due to the culture, a large number of cluster buds can be obtained from one stalk, and part of the obtained cluster buds can be continued to be subjected to subculture by a bud-propagation-bud method. The method has the advantages that the later generations can keep a stable inheritable character of a maternal line, so that flowering phase control and industrial uniform management are facilitated, and the survival rate is up to 90 percent.

The invention discloses a nurse graft molding method of lythraceae lagerstroemia trunks. The method mainly includes the steps: (1) cultivating nurse graft nursery-grown plants; (2) selecting trunk nurse graft molding ways; (3) carrying out nurse graft on the nursery-grown plants; (4) manufacturing and installing a supporting frame manually; (5) manufacturing a molding supporting template and binding the nurse graft trunks; and (6) planting the molding nurse graft nursery-grown plants. The nurse graft molding method is suitable for nurse graft molding of the lythraceae lagerstroemia trunks, can carry out nurse graft molding of the trunks no matter whether affinity is strong or weak, and healing is fast or slow, overcomes the defect that a nursery-grown plant nurse graft method can only carry out nursery-grown plant reproduction on breeds with grafts and stocks weak in the affinity and slow in healing, can carry out nurse graft on a plurality of connecting ports and connecting ports long in length of the nurse graft trunks, achieves nurse graft molding of the trunks, and overcomes the defects that the nursery-grown plant nurse graft method can only carry out the nurse graft on one connecting port and the connecting ports short in length for finishing head exchanging of single-plant nursery-grown plant breeds, and the purpose of modification of trunk shapes is not achieved.

The invention belongs to the technical field of plant reproduction and mainly provides a sarracenia asepsis sowing and culture quick reproduction method which comprises the following steps: the collection of seeds, the asepsis sowing, the enrichment culture of cespitose shoots, the culture of sound seedling, the culture of rootage, and the transplanting. The basic culture medium is as follows: selecting 1 / 6MS-1 / 3MS culture medium, and adding 20-30g / L of cane sugar or white sugar and 7-9 g / L of agaragar and having the pH of 5.6-5.8. The invention has quick reproduction speed, uniform production sturdiness and high transplanting survival rate, and is suitable to the massive production because a large number of better culture seedlings are formed in a short period.

The invention relates to a plant foliar fertilizer that is made up from fresh milk 25-33wt%, carbamide 40-45%, and potassium dihydrogen phosphate 25-33%. It contains the critical nutrition, and promotes plant reproduction balance, quickens metastasis, enhances the function of drought defying, against disease ability, etc.

The invention discloses a method for detecting the influence of microbes to plant roots and a special device for implementing the same. The method includes the following steps: (I) adding a microbe to be detected and culture solution to a box body (2); (II), putting a germination plate (1) on the top ends of partition plates inside the box body, and putting a piece of wet germination paper on the germination plate; (III), putting plant breeding materials on the germination plate, wherein each plant breeding material corresponds to one through hole of the germination plate; and (IV), covering the plant reproduction materials with a culture matrix, culturing the plant reproduction materials until the plant reproduction materials root, and observing and detecting the growth of plant roots. The method has a very broad application prospect in screening resistance varieties of plants and evaluating the prevention and control effect of bactericides on disease of plant roots.

The invention discloses a cyrtomium fortunei spore reproduction method, and belongs to the technical field of the plant reproduction. The cyrtomium fortunei spore reproduction method comprises the following steps: cyrtomium fortunei spores are collected from September to December, and impurity removal is carried out on the collected cyrtomium fortunei spores; the cyrtomium fortunei spores are disinfected by using hydrogen peroxide, disinfection is carried out on a ground substance by using a high-temperature autoclaved sterilization method, the spores are spread to a nutrition pot with a spore germination ground substance, and the nutrition pot is placed in a greenhouse; the top of the nutrition pot is covered with preservative film used for sealing so that water evaporation and fungal contamination can be prevented, and a temperature ranging from 25 to 30 DED C and an illumination intensity ranging from 2500 to 4500 lx are maintained; when two leaves grow out of a sporophyte, the sporophyte is transplanted to a seedling cultivation ground substance and is stored in a shading tent, a temperature ranges from 28 to 33 DEG C is maintained, a relative humidity ranges from 80 to 90 percent is maintained, and water is sprayed for every six to eight hours. According to the cyrtomium fortunei spore reproduction method, the operation is simple, an over 85 percent transplanting survival rate of spores seedlings is reached, the output is high with little input, the health and strong seedlings is provided for a cyrtomium fortunei artificial cultivation, and the value of practicality and popularization is high.

The invention belongs to the technical field of planting reproduction, and particularly relates to an oil-tea camellia cutting seedling transplanting seedling culture substrate. The substrate is prepared from the following raw materials of yellow soil, plant ash, chlorella powder, banyan roots, emblic leafflower leaves, pine leaves and lichen. The prepared substrate has rich and comprehensive nutrition, is rich in various trace elements, a great amount of quick-acting nutrition such as N, P and K, rich organic matters, humic acid and bioactive substances, belongs to a good seedling culture substrate, is harmless feed both achieving quick-acting and slow-acting effects, and has the advantages that the growth requirements of transplanting seedlings can be met; the water preservation and fertilizer preservation capability is high; the fertilizer effect is durable; the disease-resistant insect-killing and bacterium-inhibiting effects are achieved; the obvious seedling culture and seedling strengthening effects are achieved; the seedling culture speed is high; the seedling culture resistance is high, and the like. The contained auxins and bioactive substances can supplement the nutrition required by the seedling growth; the sprouting rate is high; the photosynthesis is enhanced; the root system growth, particularly the growth of lateral roots is promoted; the middle and later period growth of the cutting seedlings can be facilitated.

The present invention relates to a process for in vitro micropropagation of plant material comprising the steps of multiplication and elongation of shoots developed from plant propagules. The invention further comprises a process for the production of clonal seedlings plants on a large-scale ready for field development as replacements of traditional clonal gardens by ‘in vitro’ biofactories.

The invention provides a method for rapid reproduction of clerodendron trichopomum through tissue culture, and relates to the technical field of plant reproduction. The method comprises the followingsteps that 1, clerodendron trichopomum which is strong in growth and free of diseases and insect pests is selected from current-year sprouting branches and is cut into stems with the length of 2-3 cmand buds, and the stems are washed with flowing water, sterilized and cultured in an inducing medium till cluster buds are generated; 2, the cluster buds obtained after culture are irradiated with far-infrared light and ultraviolet light for 20 h, and then are cut into stems with the length of 0.6-0.8 cm; 3, the stems are inoculated into a proliferation culture medium, wherein during inoculation,the stems are flatly placed on the surface of the culture medium, and wounds are pressed into the culture medium; 4, seedlings obtained after proliferation culture are transferred into a rooting culture medium; 5, after the seedlings root, the seedlings are transferred into a seedling raising tray and naturally grows. The method is high in reproduction coefficient and survival rate, and is beneficial to achieving large-scale artificial planting.

The invention belongs to the technical field of planting reproduction and in particular relates to a method for culturing a new variety of smilax riparia with tissue mutation of flower stalks. According to the method for culturing the new variety of smilax riparia with tissue mutation of the flower stalks, smilax riparia flower stalks are adopted as a material which is soaked into ethylmethane sulfonate of an appropriate concentration for a certain time, and furthermore, by using a plant tissue culture technology, and methods of flower stalk tissue adventitious bud induction, adventitious budrooting and rejuvenation, and the like, a novel artificial cultivated variety of smilax riparia which has the characteristics of being good in single plant independence, high in single plant genetic information purity, clear in single plant source, and the like, can be obtained. The variety is stable in genetic property and can be cultivated as a strain to construct a smilax riparia mutant library.

The invention discloses a rapid garden establishment method for grafting and field planting of kiwi fruit trees, and discloses a set of systematic garden establishment method comprising the steps of modifying a garden land, selecting and storing seed branches, selecting stocks, preparing to graft, grafting and carrying out field planting. Compared with the prior art, the rapid garden establishment method has the following advantages that (1) a direct grafting and field planting method is adopted, and a management procedure of breeding seedlings in a nursery garden is avoided; a garden is formed one year ahead, and the method is suitable for a newly built kiwi fruit tree garden with soil which is sufficiently improved, so that time and labor are saved; (2) the distance between plants in the field planting process of grafting seedlings is relatively reasonable, and a space in front of the plants is large, so that the development of nursery-grown plants is facilitated; (3) only new kiwi fruit seed branches and wild seedling stocks need to be introduced in winter, so that the propagation of plant diseases and insect pests in the nursery garden in a nursery-grown plant reproduction process is reduced; the occurrence probability of the plant diseases and insect pests is reduced, and the quality of the nursery-grown plants is improved; (4) the management level is relatively high, and fertilizers and water are sufficient; after the nursery-grown plants are germinated, the nursery-grown plants can grow quickly and be sold in the same year, so that rapid ripening of gardens is facilitated.

Solanum viarum is an alkaloid producing plant of the family Solanaceae with varied therapeutic uses. In order to grow the plant in large areas; one needs to have an efficient system of vegetative multiplication, which ensures its genetic uniformity, and true to the type nature. In nature largely seeds propagate plant, which could be result of cross-pollination which may result in genetic drift. The present invention provides an efficient micropropagation system, with high level of multiplication at relatively low cost of production. The multiplication ratio was as high as 1:6 and almost 95% the plants were viable and successfully cultivated in field. The present invention provides an ideal way of mass cultivation of the selected elite plant material.

The invention discloses a foliage spraying preparation for promoting fruit setting and fruit swelling and enhancing crop stress resistance. The preparation comprises the ingredients including fulvic acid, DA-6, 2,4-D sodium salt, alpha-pimacol, gibberellin, zeatin, dimethyl sulfoxide, butylcellosolve, sodium tetraborate, zinc sulfate, ferrous sulfate, molybdenum sulfate and magnesium sulfate. After the preparation is sprayed, nutrient substances are rapidly transferred from blade surfaces and are gathered at the plant reproduction active part, and the center endogenous hormones level of fruits is improved; the cell division is promoted, the cell size is amplified, the fruit swelling is accelerated, the chloroplast development and the chlorophyll formation are promoted, the photosynthetic efficiency of leaves is improved, and the unit area yield is increased; the flowing of nutrient substances to female flowers is enhanced, the fruit development speed is accelerated, the uniform fruit development is controlled, the deformity is reduced, the fruit surface is kept fresh, bright and smooth, and the commodity rate is obviously improved; and the immunological competence of plants are excited, the resistance is improved, the occurrence of the pest and disease damage is reduced, the senescence is delayed, and the picking period and the fruit shelf period are prolonged.

The invention discloses a method for cuttage reproduction of a fresh water mangrove plant barringtonia acutangula. The method specifically comprises the following steps: preparing a cuttage bed, preparing scions, treating the scions, performing hormone treatment, performing cutttage, performing after-cuttage management, performing spraying treatment, and performing transplanting. The invention relates to the technical field of plant reproduction cultivation. According to the method for cuttage reproduction of the fresh water mangrove plant barringtonia acutangula, orthogonal experimental designing of three factors and three levels is adopted to combine different hormone soaking concentrations and different soaking times before cuttage of the scions and different hormone spraying concentrations after cuttage, an optimal barringtonia acutangula cuttage reproduction method combination is finally screened, a technical way for large-scale barringtonia acutangula culture can be obtained, anda basis can be made for providing more tree variety options for recovering sea-land ecological systems. By adopting the method, cuttage reproduction growth is rapid, the reproduction amount is large,the cost is low, excellent properties of the variety can be maintained, and technical references can be provided for improved variety seedling breeding for efficient and large-scale reproduction of the barringtonia acutangula.

The invention belongs to the technical field of plant reproduction and in particular relates to a rapid reproduction method for epimedium koreanum Nakai root segment induced buds. According to the rapid reproduction method for the epimedium koreanum Nakai root segment induced bud, rapid seedling raising of the epimedium koreanum Nakai can be completed in a mode that root segments of epimedium koreanum Nakai are adopted as a material, the material is refrigerated, soaked for 8-10 hours with a disinfectant of an appropriate concentration, put into a container and cultured at an appropriate temperature and humidity, and then complete "dormancy state" rhizomes with conical buds are induced, the whole seedling raising process is low in cost, rapid in speed, good in operability, simple, convenient and easy to popularize, the method belongs to one-step rapid reproduction technologies and can be widely applied to factory seedling raising of the epimedium koreanum Nakai, and the reproduction rate is increased by 3 times or greater and the time is shortened by 2 times or greater when being compared with those of a conventional common reproduction method.

The invention provides an isolated culture of a Neotyphodium endophyte of an Elymus canadensis host plant, wherein the endophyte reproduces asexually and enhances the agronomic characteristics of the host plant. Methods for inoculating the host plant with the endophyte, for propagating the host-endophyte combination, and for detecting the presence of the endophyte and of its metabolites within a host plant are also described.

The invention discloses a liquid composition for treating plant reproduction materials, relating to the technical field of agriculture. The liquid composition comprises the following components in percentage by mass: 10-30% of sodium lignosulfonate, 5-20% of copolymer butanol, 2-6% of dispersing agent, 3-20% of drying agent, 2-15% of starch, 3-15% of auxin and 8-20% of instantizer. The liquid composition enhances the nutrient uniformity, and is convenient to use, simple to operate and high in efficiency.

The invention discloses a culture medium for tissue culture and rapid propagation of heteromorphic Jade leaf golden flower and a culture method, and belongs to the technical field of plant propagation. The present invention adopts the tender stem of the special-shaped Jade leaf golden flower as the explant. After the explant is sterilized, a large amount of callus is produced through the induction medium, and the callus will be differentiated after being transferred to the subculture medium for 3 to 4 times. Buds are produced, and when the plants grow to 2-3 cm, they are divided into individual plants and transferred to the proliferation medium. After 30 days of cultivation, a large number of clustered buds will be induced, and then independent and complete plants will be grown through the rooting medium. The culture medium formula provided by the invention can induce differentiation of the heteromorphic Jade-leaf flower to produce a large number of plants, and solve the dilemma that the heteromorphic Jade-leaf flower is facing endangerment.

The invention discloses a method for acquiring stamen motion track, comprising the following steps: A1: delineate the stamen area from the video image of flower blooming, and determine the type and number of stamens; A2: acquire stamens for the selected stamen area The value of the RGB component and the HIS component of the area; A3: perform statistics according to the RGB component and the HIS component of the acquired stamen area; A4: segment the stamen pixel according to the grayscale statistical peak value; A5: segment the stamen pixel and determine in step A1 Carry out cluster analysis on stamen category and number, and calculate the geometric center of each stamen in the image coordinate system; A6: Repeat steps A2 to A5 for subsequent frames of images; A7: According to each frame obtained in steps A5 and A6 The geometric center of stamens in the image coordinate system, and the trajectory of each stamen in the image coordinate system is obtained. Through the method of the invention, the trajectory of each stamen is tracked, and the pollination process of the stamen is studied, so that information with guiding significance for plant propagation and breeding can be obtained.

The invention discloses a cultivation method for summer squash seedlings planted at one time and harvested for multiple times throughout a year and belongs to the field of plant propagating and breeding. The cultivation method comprises the steps of strictly selecting appropriate species; nourishing and increasing roots by comprehensively taking a plurality of measures; protecting stems and leaves, and strengthening sprouts in the lifetime; figuring out the flowering and fruit bearing rule, and mating next crop of seedlings before the flower withering period; picking and cutting the seedlings in a timely mode, and properly handling the temporal relation among the seedling mating, harvesting, storing and cutting. By means of the cultivation method, three crops of seedlings planted in an exposed mode can be continuously harvested throughout the year, and four crops of seedlings planted in a protected area can be harvested throughout the year, so that the yield of the seedlings and the seed yield per mu in a year are greatly increased, and the yield can be increased by 36%-45%. The production cost is also greatly reduced, and the high application value is achieved.

The invention relates to the technical field of plant propagation, in particular to a method for high-pressure propagation of papaya. It includes three steps: preparation for high-pressure measures, implementation of high-pressure measures, and treatment of high-pressure seedlings. The specific implementation is to select an adult hermaphrodite papaya with good performance in the field as the female parent tree, and perform high-pressure directly on the lower end of the lowest fruiting part of the female parent plant or place the papaya Cut off the upper part of the plant, induce multiple side branches to sprout from the lower part of the cut, and then apply high pressure on the side branches. Coconut bran is used as the rooting substrate. After the adventitious roots grow out after high pressure, the plants that have succeeded in high pressure can be cut off from the mother tree, moved into a nutrition bag for artificial planting, and transplanted to the field when the root system is further developed and new leaves are born. The high-pressure propagation method of papaya of the present invention has simple operation process, short required time, and its rooting rate and survival rate are both over 90%, which is a simple and efficient asexual propagation method of papaya.

The invention discloses a rapid garden establishment method for grafting and field planting of kiwi fruit trees, and discloses a set of systematic garden establishment method comprising the steps of modifying a garden land, selecting and storing seed branches, selecting stocks, preparing to graft, grafting and carrying out field planting. Compared with the prior art, the rapid garden establishment method has the following advantages that (1) a direct grafting and field planting method is adopted, and a management procedure of breeding seedlings in a nursery garden is avoided; a garden is formed one year ahead, and the method is suitable for a newly built kiwi fruit tree garden with soil which is sufficiently improved, so that time and labor are saved; (2) the distance between plants in the field planting process of grafting seedlings is relatively reasonable, and a space in front of the plants is large, so that the development of nursery-grown plants is facilitated; (3) only new kiwi fruit seed branches and wild seedling stocks need to be introduced in winter, so that the propagation of plant diseases and insect pests in the nursery garden in a nursery-grown plant reproduction process is reduced; the occurrence probability of the plant diseases and insect pests is reduced, and the quality of the nursery-grown plants is improved; (4) the management level is relatively high, and fertilizers and water are sufficient; after the nursery-grown plants are germinated, the nursery-grown plants can grow quickly and be sold in the same year, so that rapid ripening of gardens is facilitated.

The present invention provides a method of regenerating a plant, which allows stable regeneration of plants from calli; and a method of reproducing a plant, which allows stable reproduction of plants without being affected by weather, seasons or other factors. The present invention relates to a method of regenerating a plant, including a step of inducing adventitious embryos from calli; and a method of reproducing a plant, including a step of inducing adventitious embryos from calli.

The invention discloses a nurse graft molding method of lythraceae lagerstroemia trunks. The method mainly includes the steps: (1) cultivating nurse graft nursery-grown plants; (2) selecting trunk nurse graft molding ways; (3) carrying out nurse graft on the nursery-grown plants; (4) manufacturing and installing a supporting frame manually; (5) manufacturing a molding supporting template and binding the nurse graft trunks; and (6) planting the molding nurse graft nursery-grown plants. The nurse graft molding method is suitable for nurse graft molding of the lythraceae lagerstroemia trunks, can carry out nurse graft molding of the trunks no matter whether affinity is strong or weak, and healing is fast or slow, overcomes the defect that a nursery-grown plant nurse graft method can only carry out nursery-grown plant reproduction on breeds with grafts and stocks weak in the affinity and slow in healing, can carry out nurse graft on a plurality of connecting ports and connecting ports long in length of the nurse graft trunks, achieves nurse graft molding of the trunks, and overcomes the defects that the nursery-grown plant nurse graft method can only carry out the nurse graft on one connecting port and the connecting ports short in length for finishing head exchanging of single-plant nursery-grown plant breeds, and the purpose of modification of trunk shapes is not achieved.

The invention discloses a method for raising seedlings of plants of the subgenus Azalea by using immature embryos. In the method, immature embryos aged 5 months (and above) after 5 months of pollination are taken, and subjected to GA 3 After pretreatment, the seedlings are sown in the greenhouse, and the specific steps include: (1) Stripping of young embryos, (2) Pretreatment of young embryos, (3) Sowing, (4) Emergence management, (5) Seedling management and transplanting . The invention shortens the time from pollination to seedling formation of subgenus azalea, thereby effectively shortening the breeding and growth cycle of subgenus azalea, and provides a simple and easy new way for breeding and seedlings of subgenus azalea, which is simple to operate, low in cost, and can be It is popularized and applied in the introduction and domestication of plants of the subgenus Azalea, hybrid breeding, and seedling production.