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Method for rapid reproduction of clerodendron trichopomum through tissue culture

A rapid and tissue culture technology, applied in the field of plant propagation, to achieve strong adaptability, high seedling rate, and good seedling quality

Inactive Publication Date: 2018-10-26
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Haizhou Changshan has a large market demand and has broad prospects for development and utilization, but the difficulty of seedling propagation is a restrictive factor for large-scale planting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for rapid propagation of Haizhou Changshan by tissue culture, comprising the following steps:

[0026] (1) Select Changshan, Haizhou, which grows vigorously and has no pests and diseases among the germinated branches of the year, cut it into 2-3cm bud stem segments, trim off excess leaves, leave only a little petiole, rinse it with running water, and place it on an ultra-clean workbench First irradiate with ultraviolet light for 10min, then treat with 75% alcohol, then put in 10% NaClO solution and 0.1% HgCl 2 Soak in the solution for disinfection for 10 minutes, and finally rinse with sterile water for 4-6 times. After absorbing the water with sterile filter paper, insert it into the induction medium for culture. The induction medium is MS, 3mg / L IBA, 1mg / L NAA , 6mg / L 6-BA, 10g / L sucrose, 4g / L agar, first carry out dark culture, the temperature is 20-22 ℃, after the axillary buds germinate, carry out conventional light culture, the light intensity is 2800lx, ...

Embodiment 2

[0032] A method for rapid propagation of Haizhou Changshan by tissue culture, comprising the following steps:

[0033] (1) Select Changshan, Haizhou, which grows vigorously and has no pests and diseases among the germinated branches of the year, cut it into 2-3cm bud stem segments, trim off excess leaves, leave only a little petiole, rinse it with running water, and place it on an ultra-clean workbench First irradiate with ultraviolet light for 10min, then treat with 75% alcohol, then put in 10% NaClO solution and 0.1% HgCl 2 Soak in the solution for disinfection for 10 minutes, and finally rinse with sterile water for 4-6 times. After absorbing the water with sterile filter paper, insert it into the induction medium for culture. The induction medium is MS, 3mg / L IBA, 1mg / L NAA , 6mg / L 6-BA, 10g / L sucrose, 4g / L agar, first carry out dark culture, the temperature is 20-22 ℃, after the axillary buds germinate, carry out conventional light culture, the light intensity is 2600lx, ...

Embodiment 3

[0039] A method for rapid propagation of Haizhou Changshan by tissue culture, comprising the following steps:

[0040] (1) Select Changshan, Haizhou, which grows vigorously and has no pests and diseases among the germinated branches of the year, cut it into 2-3cm bud stem segments, trim off excess leaves, leave only a little petiole, rinse it with running water, and place it on an ultra-clean workbench First irradiate with ultraviolet light for 10min, then treat with 75% alcohol, then put in 10% NaClO solution and 0.1% HgCl 2 Soak in the solution for disinfection for 10 minutes, and finally rinse with sterile water for 4-6 times. After absorbing the water with sterile filter paper, insert it into the induction medium for culture. The induction medium is MS, 3mg / L IBA, 1mg / L NAA , 6mg / L 6-BA, 10g / L sucrose, 4g / L agar, first carry out dark culture, the temperature is 20-22 ℃, after the axillary buds germinate, carry out conventional light culture, the light intensity is 2500lx, th...

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PUM

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Abstract

The invention provides a method for rapid reproduction of clerodendron trichopomum through tissue culture, and relates to the technical field of plant reproduction. The method comprises the followingsteps that 1, clerodendron trichopomum which is strong in growth and free of diseases and insect pests is selected from current-year sprouting branches and is cut into stems with the length of 2-3 cmand buds, and the stems are washed with flowing water, sterilized and cultured in an inducing medium till cluster buds are generated; 2, the cluster buds obtained after culture are irradiated with far-infrared light and ultraviolet light for 20 h, and then are cut into stems with the length of 0.6-0.8 cm; 3, the stems are inoculated into a proliferation culture medium, wherein during inoculation,the stems are flatly placed on the surface of the culture medium, and wounds are pressed into the culture medium; 4, seedlings obtained after proliferation culture are transferred into a rooting culture medium; 5, after the seedlings root, the seedlings are transferred into a seedling raising tray and naturally grows. The method is high in reproduction coefficient and survival rate, and is beneficial to achieving large-scale artificial planting.

Description

technical field [0001] The invention relates to the technical field of plant propagation, in particular to a method for rapidly propagating Haizhou Changshan by tissue culture. Background technique [0002] Haizhou Changshan is also known as dove dish and pickled flower tree. It is also called stinky phoenix tree because of its large leaves. It is a deciduous shrub or small tree of the genus Daqing in the Verbenaceae family. Haizhou Changshan is a woody wild vegetable with a unique flavor, and it is also a widely used medicinal plant. It is used in a large amount in the Chinese herbal medicine market. In addition, a tree in Changshan, Haizhou has flowers and fruits coexisting, and the flowering and fruiting period is long, and the color is bright. It is a good garden plant for viewing flowers and fruits; The arsenic tolerance and enrichment ability are strong, and it is an ideal restoration tree species for arsenic-contaminated areas. Haizhou Changshan has a large market d...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G17/00A01G31/00A01G24/28A01G24/15A01G24/27A01G24/20
CPCA01G17/005A01G31/00A01H4/001A01H4/005A01G24/15A01G24/20A01G24/27A01G24/28
Inventor 张应华宋婷郭晋木霖许彬许俊强毕保良吴兴恩吕霞董玉梅张国平
Owner YUNNAN AGRICULTURAL UNIVERSITY
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