Method for performing regeneration propagation on sterile stalks of butterfly orchids

A technology of phalaenopsis and pedicel, which is applied in the field of seedling cultivation, can solve the problems of poor ornamental value of native species, achieve the effect of reducing production cost and huge sales volume

Inactive Publication Date: 2014-10-08
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are about 70 kinds of native species in the world, and there are more than 530 varieties through hybridization, but the ornamental value of native species is poor, and most of the varieties used in commercial mass production are hybrids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Cut the Phalaenopsis pedicels with side buds without pests and diseases into 2-3cm segments, remove the bracts on the outer layer of the buds with tweezers and other tools, add 4-6ml Tween 20 and stir well, then pour in tap water Rinse under water for more than 30 minutes, transfer to the ultra-clean workbench and disinfect with 70% to 75% alcohol for 30 seconds, then treat with sterile water for 2 to 3 times, add 2% NaClO solution for 5 to 6 minutes, and then rinse with sterile water for 4 ~5 times, use sterile filter paper to blot the surface moisture, cut off the surface of the flower stalk that is in contact with the disinfectant, and inoculate it into the primary culture medium for cultivation. The primary medium is: the above-mentioned step (1) The primary medium is: 1 / 2MS +5.0mg / L6-BA+0.5mg / LNAA+20g / L sucrose+6 / L agar+400mg / L citric acid+40g / L banana puree, pH value is 5.8, culture condition The method is: temperature 25±2°C, dark culture for 7-10 days in ...

Embodiment 2

[0019] The difference with embodiment 1 is:

[0020] The primary medium in the step (1) is: 1MS +5.0mg / L6-BA+0.5mg / LNAA+20g / L sucrose+6 / L agar+400mg / L vitamin C+40g / L potato juice, The pH value is 5.8.

Embodiment 3

[0022] The difference with embodiment 1 is:

[0023] The primary medium in the step (1) is: 1 / 2MS + 3mg / L6-BA + 0.3mg / LNAA + 25-30g / L sucrose 6.5g / L agar + 350mg / L citric acid + 35g / L Banana puree; the subculture medium in the step (2) is: 1 / 2MS +5mg / L6-BA+0.4mg / LNAA+25~30g / L sucrose+6.5g / L agar+400mg / L activated carbon +35g / L coconut milk; the rooting medium in the step (3) is: 1 / 2MS+1.5mg / L NAA+30g / L sucrose+6 g / L agar+3g / L activated carbon+40g / L Mashed bananas; pH 5.6.

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PUM

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Abstract

The invention discloses a method for performing regeneration propagation on sterile stalks of butterfly orchids, relates to the technical field of seedling cultivation, mainly relates to a quick butterfly orchid plant reproduction technology, and in particular relates to a plant regeneration method for the butterfly orchid species. The method comprises the steps of cutting a stalk axillary bud stem with a certain length from a robust mother butterfly orchid plant, taking the stalk axillary bud stem as an explant material, wherein an axillary bud is opened and a leaf is in jade green and glossy after the sterile stalk is subjected to primary culture; after the axillary bud is cut down, culturing the stalk on a subculture medium, then continuously forming bud points at an incision of the axillary bud of the stalk to gradually form another axillary bud, continuing to cut down the axillary bud after the axillary bud is opened, and inoculating the stalk on the subculture medium again for subculture, wherein due to the culture, a large number of cluster buds can be obtained from one stalk, and part of the obtained cluster buds can be continued to be subjected to subculture by a bud-propagation-bud method. The method has the advantages that the later generations can keep a stable inheritable character of a maternal line, so that flowering phase control and industrial uniform management are facilitated, and the survival rate is up to 90 percent.

Description

technical field [0001] The invention relates to the technical field of seedling cultivation, mainly relates to the rapid propagation technology of Phalaenopsis plants, and in particular relates to a plant regeneration method of Phalaenopsis varieties. Background technique [0002] Phalaenopsis, also known as Phalaenopsis, belongs to tropical aerial orchids, and the Orchidaceae Phalaenopsis belongs to perennial herbaceous plants. The origin is in Assam, Myanmar, Philippines, Taiwan and other Asian tropical regions. Its reproduction is slow, the growth cycle is long, the plant type is beautiful, the flowers are large and colorful, the flower shape is unique, and the flowering period is long-lasting. It is deeply loved by people from all over the world. One of the four most valuable ornamental tropical orchids. There are about 70 kinds of native species in the world, and there are more than 530 varieties through hybridization, but the ornamental value of native species is poor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 何炎红白玉娥叶冬梅
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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