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Cyrtomium fortunei spore reproduction method

A technology of spores and spores, applied in the field of plant propagation, can solve the problems of low spore germination rate and survival rate, unsatisfied market demand, lack of wild spore resources, etc., to achieve high germination rate and survival rate, high practical promotion value, the effect of preventing pollution

Inactive Publication Date: 2015-05-13
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the indiscriminate harvesting and excavation of wild crocus resources in recent years, the current wild crocus resources are lacking and the market demand cannot be met. Therefore, it is urgent to carry out artificial planting of crocuses to meet the growing market demand
[0004] In the natural state, the reproduction of spores is generally through spore reproduction. Under such conditions, due to the influence of various external biological or abiotic factors, the germination rate and survival rate of the spores are very low. However, although tissue culture and cuttings It can also be propagated, but it requires relatively high technical content, and the survival rate of cuttings is low, so it is inconvenient to promote

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] In mid-October, when the sporophyll spores are six mature, collect the spores, put the spores on a piece of hard and smooth paper, then tilt the paper to 15-20°, shake the paper gently, and remove the upper layer of spores Impurities such as capsule shells, cyst group lids, and scales were removed, and then the pure spores were stored in a refrigerator at 4°C for later use.

[0021] The spores were sterilized by 10% hydrogen peroxide for 5 minutes, and the spore germination substrate and the seedling cultivation substrate were sterilized for 30 minutes with a pressure of 103-137KPa at the same time, and then the spores were spread in the spore germination substrate. The composition of the spore germination substrate is river sand: peat: pastoral soil (2:1:1, volume ratio), and the composition of the seedling cultivation substrate is perlite: peat (1:2, volume ratio).

[0022] Place the spore-spread nutrient bowl in the greenhouse, cover the nutrient bowl with plastic wr...

Embodiment 2

[0025] In mid-November, when the sporophyll spores are six mature, collect the spores, put the spores on a piece of slightly hard and smooth paper, then tilt the paper to 15-20°, shake the paper gently, and remove the upper layer of the spores. Lightly remove impurities such as sporangia, capsules, scales, etc., and then store the pure spores in a refrigerator at 4°C for later use.

[0026] The spores were sterilized with 10% hydrogen peroxide for 5 minutes, and the spore germination substrate and the seedling cultivation substrate were not sterilized by autoclaving, and the spores were sown in the spore germination substrate. The composition of the spore germination substrate is river sand: peat: pastoral soil (2:1:1, volume ratio), and the composition of the seedling cultivation substrate is perlite: peat (1:2, volume ratio).

[0027] Place the spore-spread nutrient bowl in the greenhouse, cover the nutrient bowl with plastic wrap to seal it to prevent water loss and fungal ...

Embodiment 3

[0030] In early November, when the sporophyll spores were six mature, the spores were collected without impurity removal, and the spores were stored in a refrigerator at 4°C for later use.

[0031] The spores are sterilized by 10% hydrogen peroxide for 30 minutes, and the spore germination substrate and the seedling cultivation substrate are sterilized for 30 minutes by an autoclaving method with a pressure of 103-137 KPa, and then the spores are spread in the spore germination substrate. The composition of the spore germination substrate is river sand: peat: pastoral soil (2:1:1, volume ratio), and the composition of the seedling cultivation substrate is perlite: peat (1:2, volume ratio).

[0032] Place the spore-spread nutrient bowl in the greenhouse, cover the nutrient bowl with plastic wrap to seal it to prevent water loss and fungal contamination, the temperature for spore germination and cultivation is 25-30°C, and the light intensity is 2500-4500lx.

[0033] Generally, ...

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Abstract

The invention discloses a cyrtomium fortunei spore reproduction method, and belongs to the technical field of the plant reproduction. The cyrtomium fortunei spore reproduction method comprises the following steps: cyrtomium fortunei spores are collected from September to December, and impurity removal is carried out on the collected cyrtomium fortunei spores; the cyrtomium fortunei spores are disinfected by using hydrogen peroxide, disinfection is carried out on a ground substance by using a high-temperature autoclaved sterilization method, the spores are spread to a nutrition pot with a spore germination ground substance, and the nutrition pot is placed in a greenhouse; the top of the nutrition pot is covered with preservative film used for sealing so that water evaporation and fungal contamination can be prevented, and a temperature ranging from 25 to 30 DED C and an illumination intensity ranging from 2500 to 4500 lx are maintained; when two leaves grow out of a sporophyte, the sporophyte is transplanted to a seedling cultivation ground substance and is stored in a shading tent, a temperature ranges from 28 to 33 DEG C is maintained, a relative humidity ranges from 80 to 90 percent is maintained, and water is sprayed for every six to eight hours. According to the cyrtomium fortunei spore reproduction method, the operation is simple, an over 85 percent transplanting survival rate of spores seedlings is reached, the output is high with little input, the health and strong seedlings is provided for a cyrtomium fortunei artificial cultivation, and the value of practicality and popularization is high.

Description

technical field [0001] The invention relates to the technical field of plant propagation, in particular to a method for propagating spores. Background technique [0002] Guanzhong (Dryopteriscrassirhizoma Nakai) is a perennial herb belonging to the family Pteridaceae, with a height of 50-100cm. The rhizome is thick and oblique, with more hard petiole residues and black fine roots, densely covered with dark brown, long lanceolate large scales. Leaves clustered at the top of the rhizome; petiole 10-25cm long, densely brown strips to subulate narrow scales above the base to the rachis, herbaceous leaves, oblanceolate, 60-100cm long, 20-25cm wide at the middle part, Bipinnatisect or deeply lobed; pinna sessile, lobes closely connected, oblong, rounded or truncated, nearly entire or apex with obtuse serrations; upper part dark green, lower part light green, lateral veins pinnate fork. The sporophyll is the same shape as the vegetative leaf. The sporangium is born on the pinna ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00
CPCA01G22/00
Inventor 李勇超周修任杨靖代磊简在友
Owner HENAN INST OF SCI & TECH
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