A kind of special-shaped jade leaf golden flower tissue culture rapid propagation medium and culture method

A technology of Jade Leaf Golden Flower and tissue culture rapid propagation, which is applied in the field of plant propagation, can solve the problems of low differentiation rate and survival rate, and achieve the goal of promoting bud germination, avoiding excessive growth or excessive growth, and increasing the induction rate and the number of buds Effect

Active Publication Date: 2022-06-07
黔东南苗族侗族自治州林业科学研究所
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Problems solved by technology

[0004] Although a lot of methods for the tissue culture of Heterophylla heteromorpha have been disclosed, the differentiation rate and survival rate are often low in the process of tissue culture using these methods, and how to effectively and rapidly improve the induction differentiation rate and survival rate, and then improve the number of reproduction of the heteromorphic Jade leaf golden flower, solve its dilemma of extinction and achieve the purpose of mass production and application, which has become a difficult problem to be solved urgently by those skilled in the art

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  • A kind of special-shaped jade leaf golden flower tissue culture rapid propagation medium and culture method

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Experimental program
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Effect test

Embodiment 1

[0034] (1) Preparation of culture medium:

[0035] Induction medium: 0.05 mg of 6-benzylaminopurine and 0.01 mg of naphthaleneacetic acid were added per 1 liter of MS medium to adjust the pH to 5.7.

[0036] Subsequent medium: 3 mg of 6-benzylaminopurine, 0.15 mg of kinetin and 0.01 mg of naphthaleneacetic acid were added per 1 liter of MS medium to adjust the pH to 5.7.

[0037] Proliferation medium: 3 mg of 6-benzylaminopurine, 0.15 mg of kinetin and 0.01 mg of indoleacetic acid were added to each 1 liter of MS medium to adjust the pH to 5.7.

[0038] Rooting medium: 0.1 mg of indole butyric acid and 0.5 g of activated carbon were added to each 1 liter of 1 / 2 MS medium to adjust the pH to 5.7.

[0039] (2) Disinfection of explants: collect the tender stems of Heteromorpha japonica as explants, rinse them with running water for 4 hours, put them into an ultra-clean workbench for ultraviolet sterilization for 30 minutes, and then soak the stem segments in 75% ethanol for 30 s...

Embodiment 2

[0045] (1) Culture medium configuration:

[0046] Induction medium: 0.1 mg of 6-benzylaminopurine and 0.05 mg of naphthaleneacetic acid were added to each 1 liter of MS medium to adjust the pH to 5.7.

[0047] Subsequent medium: 4 mg of 6-benzylaminopurine, 0.25 mg of kinetin and 0.02 mg of naphthalene acetic acid were added to each 1 liter of MS medium to adjust the pH to 5.7.

[0048] Proliferation medium: 4 mg of 6-benzylaminopurine, 0.25 mg of kinetin and 0.02 mg of indoleacetic acid were added per 1 liter of MS medium to adjust the pH to 5.7.

[0049] Rooting medium: add 0.15 mg of indole butyric acid and 1 g of activated carbon to each 1 liter of 1 / 2 MS medium to adjust the pH to 5.7.

[0050] (2) Disinfection of explants: collect the tender stems of Heteromorpha japonica as explants, rinse them with running water for 4 hours, put them into an ultra-clean workbench for ultraviolet sterilization for 30 minutes, and then soak the stem segments in 75% ethanol for 30 second...

Embodiment 3

[0056] (1) Culture medium configuration:

[0057] Induction medium: 0.15 mg of 6-benzylaminopurine and 0.1 mg of naphthaleneacetic acid were added to each 1 liter of MS medium to adjust the pH to 5.7.

[0058] Subsequent medium: 5 mg of 6-benzylaminopurine, 0.35 mg of kinetin and 0.03 mg of naphthaleneacetic acid were added to each 1 liter of MS medium to adjust the pH to 5.7.

[0059] Proliferation medium: 5 mg of 6-benzylaminopurine, 0.35 mg of kinetin, and 0.03 mg of indoleacetic acid were added per 1 liter of MS medium to adjust the pH to 5.7.

[0060] Rooting medium: 0.2 mg of indole butyric acid and 1.5 g of activated carbon were added to each 1 liter of 1 / 2 MS medium to adjust the pH to 5.7.

[0061] (2) Disinfection of explants: collect the tender stems of Heteromorpha japonica as explants, rinse them with running water for 4 hours, put them into an ultra-clean workbench for ultraviolet sterilization for 30 minutes, and then soak the stem segments in 75% ethanol for 3...

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Abstract

The invention discloses a culture medium for tissue culture and rapid propagation of heteromorphic Jade leaf golden flower and a culture method, and belongs to the technical field of plant propagation. The present invention adopts the tender stem of the special-shaped Jade leaf golden flower as the explant. After the explant is sterilized, a large amount of callus is produced through the induction medium, and the callus will be differentiated after being transferred to the subculture medium for 3 to 4 times. Buds are produced, and when the plants grow to 2-3 cm, they are divided into individual plants and transferred to the proliferation medium. After 30 days of cultivation, a large number of clustered buds will be induced, and then independent and complete plants will be grown through the rooting medium. The culture medium formula provided by the invention can induce differentiation of the heteromorphic Jade-leaf flower to produce a large number of plants, and solve the dilemma that the heteromorphic Jade-leaf flower is facing endangerment.

Description

technical field [0001] The invention relates to the technical field of plant reproduction, in particular to a tissue culture fast-propagation medium and a culturing method for heteromorphic jade leaf golden flower. Background technique [0002] Heteromorpha is a climbing shrub of the Rubiaceae family. The 5 lobes of the calyx of this species are all expanded into white petals, and the structure is very peculiar. The special-shaped jade leaf golden flower also has unique medicinal value, its clearing heat and detoxification, anti-inflammatory and analgesic effects are obvious, and its economic and scientific research value potential is huge. This species is evergreen in all seasons, elegant in posture, beautiful in flower shape and strong in decoration. It is very suitable for garden landscaping, flower bed decoration, garden beautification and home pot planting. [0003] Heteromorpha japonica has been listed as a national class I key protected plant and an endangered specie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陶光林姚祖娟胡岑龙杨东红石春发李彬唐绍平杨秀益
Owner 黔东南苗族侗族自治州林业科学研究所
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