A kind of special-shaped jade leaf golden flower tissue culture rapid propagation medium and culture method
A technology of Jade Leaf Golden Flower and tissue culture rapid propagation, which is applied in the field of plant propagation, can solve the problems of low differentiation rate and survival rate, and achieve the goal of promoting bud germination, avoiding excessive growth or excessive growth, and increasing the induction rate and the number of buds Effect
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Embodiment 1
[0034] (1) Preparation of culture medium:
[0035] Induction medium: 0.05 mg of 6-benzylaminopurine and 0.01 mg of naphthaleneacetic acid were added per 1 liter of MS medium to adjust the pH to 5.7.
[0036] Subsequent medium: 3 mg of 6-benzylaminopurine, 0.15 mg of kinetin and 0.01 mg of naphthaleneacetic acid were added per 1 liter of MS medium to adjust the pH to 5.7.
[0037] Proliferation medium: 3 mg of 6-benzylaminopurine, 0.15 mg of kinetin and 0.01 mg of indoleacetic acid were added to each 1 liter of MS medium to adjust the pH to 5.7.
[0038] Rooting medium: 0.1 mg of indole butyric acid and 0.5 g of activated carbon were added to each 1 liter of 1 / 2 MS medium to adjust the pH to 5.7.
[0039] (2) Disinfection of explants: collect the tender stems of Heteromorpha japonica as explants, rinse them with running water for 4 hours, put them into an ultra-clean workbench for ultraviolet sterilization for 30 minutes, and then soak the stem segments in 75% ethanol for 30 s...
Embodiment 2
[0045] (1) Culture medium configuration:
[0046] Induction medium: 0.1 mg of 6-benzylaminopurine and 0.05 mg of naphthaleneacetic acid were added to each 1 liter of MS medium to adjust the pH to 5.7.
[0047] Subsequent medium: 4 mg of 6-benzylaminopurine, 0.25 mg of kinetin and 0.02 mg of naphthalene acetic acid were added to each 1 liter of MS medium to adjust the pH to 5.7.
[0048] Proliferation medium: 4 mg of 6-benzylaminopurine, 0.25 mg of kinetin and 0.02 mg of indoleacetic acid were added per 1 liter of MS medium to adjust the pH to 5.7.
[0049] Rooting medium: add 0.15 mg of indole butyric acid and 1 g of activated carbon to each 1 liter of 1 / 2 MS medium to adjust the pH to 5.7.
[0050] (2) Disinfection of explants: collect the tender stems of Heteromorpha japonica as explants, rinse them with running water for 4 hours, put them into an ultra-clean workbench for ultraviolet sterilization for 30 minutes, and then soak the stem segments in 75% ethanol for 30 second...
Embodiment 3
[0056] (1) Culture medium configuration:
[0057] Induction medium: 0.15 mg of 6-benzylaminopurine and 0.1 mg of naphthaleneacetic acid were added to each 1 liter of MS medium to adjust the pH to 5.7.
[0058] Subsequent medium: 5 mg of 6-benzylaminopurine, 0.35 mg of kinetin and 0.03 mg of naphthaleneacetic acid were added to each 1 liter of MS medium to adjust the pH to 5.7.
[0059] Proliferation medium: 5 mg of 6-benzylaminopurine, 0.35 mg of kinetin, and 0.03 mg of indoleacetic acid were added per 1 liter of MS medium to adjust the pH to 5.7.
[0060] Rooting medium: 0.2 mg of indole butyric acid and 1.5 g of activated carbon were added to each 1 liter of 1 / 2 MS medium to adjust the pH to 5.7.
[0061] (2) Disinfection of explants: collect the tender stems of Heteromorpha japonica as explants, rinse them with running water for 4 hours, put them into an ultra-clean workbench for ultraviolet sterilization for 30 minutes, and then soak the stem segments in 75% ethanol for 3...
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